enzymatic digestion  (New England Biolabs)


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  • 94
    Name:
    PNGase F native
    Description:
    PNGase F native 75 000 units
    Catalog Number:
    p0704l
    Price:
    754
    Size:
    75 000 units
    Category:
    Glycosidases
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    Structured Review

    New England Biolabs enzymatic digestion
    PNGase F native
    PNGase F native 75 000 units
    https://www.bioz.com/result/enzymatic digestion/product/New England Biolabs
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    enzymatic digestion - by Bioz Stars, 2020-08
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Overexpression of Ebola virus envelope GP1 protein
    Article Snippet: .. For N-linked glycan removal, GP1 protein in denaturing buffer was first denatured by incubation at 100 °C for 10 min, PNGase F (New England Biolabs, Beverly, MA) was then added and digestion was performed at 37 °C for 2 h. Treated or untreated samples were then analyzed by Western blot under reducing conditions. ..

    Incubation:

    Article Title: Overexpression of Ebola virus envelope GP1 protein
    Article Snippet: .. For N-linked glycan removal, GP1 protein in denaturing buffer was first denatured by incubation at 100 °C for 10 min, PNGase F (New England Biolabs, Beverly, MA) was then added and digestion was performed at 37 °C for 2 h. Treated or untreated samples were then analyzed by Western blot under reducing conditions. ..

    Article Title: Impaired Autoproteolytic Cleavage of mCLCA6, a Murine Integral Membrane Protein Expressed in Enterocytes, Leads to Cleavage at the Plasma Membrane Instead of the Endoplasmic Reticulum
    Article Snippet: .. Cell lysates were incubated with Endo H or PNGase F (New England Biolabs) prior to SDS-PAGE as described previously ( ). .. Transfected cells were surface biotinylated as described by with minor modifications.

    other:

    Article Title: Identification of the N-linked glycosylation sites of vitamin K-dependent carboxylase and the effect of glycosylation on carboxylase function
    Article Snippet: Removal of the oligosaccharides from asparagines by PNGase F converts the asparagine (114 Da) to aspartate (115 Da).

    Article Title: Full-length core sequence dependent complex-type glycosylation of hepatitis C virus E2 glycoprotein
    Article Snippet: The glycan type on different E2 species expressed from pCEH-2 (1-730) was then analyzed by testing their sensitivity to PNGase F and Endo H. PNGase F hydrolyzes all types of N-glycan chains from glycopeptides and glycoproteins unless they carry α-1-3 linked core fucose residues present in insect and plant glycoproteins[ ], while Endo H cleaves only high mannose structures and hybrid structures on N-linked oligosaccharides of glycoproteins[ ].

    Article Title: A Redundant Role of Human Thyroid Peroxidase Propeptide for Cellular, Enzymatic, and Immunological Activity
    Article Snippet: Subsequently, the intracellular and surface fractions were subjected to deglycosylation with Endo H or PNGase F ( ).

    SDS Page:

    Article Title: Impaired Autoproteolytic Cleavage of mCLCA6, a Murine Integral Membrane Protein Expressed in Enterocytes, Leads to Cleavage at the Plasma Membrane Instead of the Endoplasmic Reticulum
    Article Snippet: .. Cell lysates were incubated with Endo H or PNGase F (New England Biolabs) prior to SDS-PAGE as described previously ( ). .. Transfected cells were surface biotinylated as described by with minor modifications.

    Centrifugation:

    Article Title: CEACAM1 regulates TIM–3–mediated tolerance and exhaustion
    Article Snippet: .. Supernatants were collected by centrifugation, and half of each was digested with PNGase F (New England Biolabs). .. Samples were separated by SDS–PAGE and the TIM-3–Ig fusion protein-specific binding proteins were detected by immunoblot or sliver staining.

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  • 92
    New England Biolabs enzymatic digestion
    Enzymatic Digestion, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymatic digestion/product/New England Biolabs
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    enzymatic digestion - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

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