Structured Review

Miltenyi Biotec enzymatic digestion
Enzymatic Digestion, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/enzymatic digestion/product/Miltenyi Biotec
Average 92 stars, based on 22 article reviews
Price from $9.99 to $1999.99
enzymatic digestion - by Bioz Stars, 2020-08
92/100 stars

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Selection:

Article Title: Definitive activation of endogenous antitumor immunity by repetitive cycles of cyclophosphamide with interspersed Toll-like receptor agonists
Article Snippet: .. CD45 cell enrichment for tumor digests Tumor-infiltrating leukocytes released by enzymatic digestion from resected 4T1 tumors of TB mice were subjected to CD45 enrichment by positive selection (130-052-301, CD45 MicroBeads mouse kit, Miltenyi Biotec), following manufacture's instruction. .. In brief, total tumor-infiltrating leukocytes were adjusted to 1×107 cells in 90 ul isolation buffer (0.5% BSA and 2 mM EDTA in PBS, pH 7.2), and 10 ul CD45 MicroBeads were added.

Homogenization:

Article Title: Inflammaging increases susceptibility to cigarette smoke-induced COPD
Article Snippet: .. Lung homogenization was performed via enzymatic digestion and mechanical dissociation steps using a lung dissociation buffer and the gentleMACS Dissociator (both from Miltenyi Biotec, Bergisch Gladbach, Germany). ..

Isolation:

Article Title: Cerebral cavernous malformations arise from endothelial gain of MEKK3-KLF2/4 signaling
Article Snippet: .. Isolation of cerebellar endothelial cells Cerebellar endothelial cells were isolated through enzymatic digestion followed by separation using magnetic-activated cell sorting (MACS MS system, Miltenyi Biotec). .. Mice were first anesthetized with Avertin (Sigma Aldrich, T48402) and perfused with sterile phosphate buffered saline (PBS).

Mouse Assay:

Article Title: Definitive activation of endogenous antitumor immunity by repetitive cycles of cyclophosphamide with interspersed Toll-like receptor agonists
Article Snippet: .. CD45 cell enrichment for tumor digests Tumor-infiltrating leukocytes released by enzymatic digestion from resected 4T1 tumors of TB mice were subjected to CD45 enrichment by positive selection (130-052-301, CD45 MicroBeads mouse kit, Miltenyi Biotec), following manufacture's instruction. .. In brief, total tumor-infiltrating leukocytes were adjusted to 1×107 cells in 90 ul isolation buffer (0.5% BSA and 2 mM EDTA in PBS, pH 7.2), and 10 ul CD45 MicroBeads were added.

Mass Spectrometry:

Article Title: Cerebral cavernous malformations arise from endothelial gain of MEKK3-KLF2/4 signaling
Article Snippet: .. Isolation of cerebellar endothelial cells Cerebellar endothelial cells were isolated through enzymatic digestion followed by separation using magnetic-activated cell sorting (MACS MS system, Miltenyi Biotec). .. Mice were first anesthetized with Avertin (Sigma Aldrich, T48402) and perfused with sterile phosphate buffered saline (PBS).

FACS:

Article Title: Cerebral cavernous malformations arise from endothelial gain of MEKK3-KLF2/4 signaling
Article Snippet: .. Isolation of cerebellar endothelial cells Cerebellar endothelial cells were isolated through enzymatic digestion followed by separation using magnetic-activated cell sorting (MACS MS system, Miltenyi Biotec). .. Mice were first anesthetized with Avertin (Sigma Aldrich, T48402) and perfused with sterile phosphate buffered saline (PBS).

Magnetic Cell Separation:

Article Title: Cerebral cavernous malformations arise from endothelial gain of MEKK3-KLF2/4 signaling
Article Snippet: .. Isolation of cerebellar endothelial cells Cerebellar endothelial cells were isolated through enzymatic digestion followed by separation using magnetic-activated cell sorting (MACS MS system, Miltenyi Biotec). .. Mice were first anesthetized with Avertin (Sigma Aldrich, T48402) and perfused with sterile phosphate buffered saline (PBS).

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    Miltenyi Biotec enzymatic digestion
    Enzymatic Digestion, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymatic digestion/product/Miltenyi Biotec
    Average 92 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    enzymatic digestion - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

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