enzymatic digestion  (Lonza)


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  • 99

    Structured Review

    Lonza enzymatic digestion
    Enzymatic Digestion, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymatic digestion/product/Lonza
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    enzymatic digestion - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Antigen Loading (e.g., Glutamic Acid Decarboxylase 65) of Tolerogenic DCs (tolDCs) Reduces Their Capacity to Prevent Diabetes in the Non-Obese Diabetes (NOD)-Severe Combined Immunodeficiency Model of Adoptive Cotransfer of Diabetes As Well As in NOD Mice
    Article Snippet: .. The fresh isolated cells were subsequently cultured (37°C, 5% CO2 ) in Petri cell-culture dishes (90 mm in diameter) in complete prewarmed l -glutamine containing RPMI-1640 (Lonza, Verviers, Belgium) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco-Life Technologies, Paisley, UK), 100× diluted NEM-Non-essential amino acid (Sigma-Aldrich), 1 µM sodium pyruvate (Sigma-Aldrich), 50 IU/mL penicillin, 50 µg/mL streptomycin, and 50 µM 2-β-mercaptoethanol. .. In other experiments, cells were cultured in serum-free (SF) CellGro medium (CellGenix, Freiburg, Germany) with the same supplements as for RPMI-1640 except FBS.

    Incubation:

    Article Title: Complement C3 is a novel modulator of the anti-factor VIII immune response
    Article Snippet: .. Co-localization of FVIII and C3b by immunofluorescence FVIII (50 nM, Helixate®) or buffer alone were incubated in the presence of 20% HIS or NS for 1 h at 37°C in RPMI-1640. ..

    Cell Culture:

    Article Title: A novel pathway of LPS uptake through syndecan-1 leading to pyroptotic cell death
    Article Snippet: .. LLC and B16F10 cells were cultured in RPMI 1640 Medium (LONZA) with heat-inactivated fetal bovine serum (FBS), supplemented with penicillin/streptomycin (1:100) at 37 ˚C, 5% CO2 . ..

    Article Title: Antigen Loading (e.g., Glutamic Acid Decarboxylase 65) of Tolerogenic DCs (tolDCs) Reduces Their Capacity to Prevent Diabetes in the Non-Obese Diabetes (NOD)-Severe Combined Immunodeficiency Model of Adoptive Cotransfer of Diabetes As Well As in NOD Mice
    Article Snippet: .. The fresh isolated cells were subsequently cultured (37°C, 5% CO2 ) in Petri cell-culture dishes (90 mm in diameter) in complete prewarmed l -glutamine containing RPMI-1640 (Lonza, Verviers, Belgium) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco-Life Technologies, Paisley, UK), 100× diluted NEM-Non-essential amino acid (Sigma-Aldrich), 1 µM sodium pyruvate (Sigma-Aldrich), 50 IU/mL penicillin, 50 µg/mL streptomycin, and 50 µM 2-β-mercaptoethanol. .. In other experiments, cells were cultured in serum-free (SF) CellGro medium (CellGenix, Freiburg, Germany) with the same supplements as for RPMI-1640 except FBS.

    Generated:

    Article Title: CCR5 antagonism by maraviroc inhibits Hodgkin lymphoma microenvironment interactions and xenograft growth
    Article Snippet: .. To prepare conditioned medium (CM) from cHL cell lines, cells were seeded at 2.0×105 /ml in RPMI-1640 plus 10% FCS, and the medium was collected after 72 h. Human bone marrow-derived and adipose tissue-derived mesenchymal stromal cells (MSCs) (BM-MSCs and AT-MSC, respectively) were purchased from Lonza (Verviers, Belgium). cHL-MSCs from frozen lymph nodes were generated as described in Online Supplementary Methods . .. BM-MSCs, AT-MSC and cHL-MSCs were maintained in Mesenchymal Stem Cell Growth Medium Bulletkit (Lonza) to avoid differentiation.

    other:

    Article Title: mTOR ATP-competitive inhibitor INK128 inhibits neuroblastoma growth via blocking mTORC signaling
    Article Snippet: Briefly, cells were seeded in RPMI-1640 containing 10% FBS and 25 mM of glucose overnight.

    Article Title: Dipeptidyl Peptidase IV Is a Human and Murine Neutrophil Chemorepellent
    Article Snippet: RPMI 1640 does not contain proteins, and BSA and human albumin do not have a proline or an alanine as the second amino acid.

    Article Title: The invasion process of bovine erythrocyte by Babesia divergens: knowledge from an in vitro assay
    Article Snippet: Of the three media tested, RPMI 1640 + 20% FCS and RPMI 1640 exhibited the same invasion efficiency.

    Immunofluorescence:

    Article Title: Complement C3 is a novel modulator of the anti-factor VIII immune response
    Article Snippet: .. Co-localization of FVIII and C3b by immunofluorescence FVIII (50 nM, Helixate®) or buffer alone were incubated in the presence of 20% HIS or NS for 1 h at 37°C in RPMI-1640. ..

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  • 92
    Lonza enzymatic digestion
    Enzymatic Digestion, supplied by Lonza, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymatic digestion/product/Lonza
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    enzymatic digestion - by Bioz Stars, 2020-08
    92/100 stars
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