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Becton Dickinson enzymatic digestion
Enzymatic Digestion, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/enzymatic digestion/product/Becton Dickinson
Average 93 stars, based on 52 article reviews
Price from $9.99 to $1999.99
enzymatic digestion - by Bioz Stars, 2020-08
93/100 stars

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In Vivo:

Article Title: Stearoyl-CoA desaturase-1 is a key factor for lung cancer-initiating cells
Article Snippet: .. In vivo studies For xenograft assay, single-cell suspension was obtained by enzymatic digestion of the spheroids using different cell numbers in a volume of 100 μ l of a 50% GFR Matrigel (BD Biosciences, Franklin Lakes, NJ, USA). .. In particular, Pe o/11 and NCI-H460 spheroids generated for 48 h in the absence or in the presence of SCD1 inhibitor (500 nM) were injected subcutaneously into the right flank of ten CD1 nude mice for each group.

Filtration:

Article Title: Effect of red light and near infrared laser on the generation of reactive oxygen species in primary dermal fibroblasts
Article Snippet: .. Briefly, donated tissue was subjected to enzymatic digestion with collagenase solution, followed by high-speed centrifugation and filtration of the infranatant through a 40 μm filter (BD Biosciences, 352,340) to obtain the stromal vascular fraction (SVF) containing high numbers of blood cells with traces of endothelial cells, fibroblasts, pericytes and stem cells. .. Cell pellet containing the SVF was re-suspended in Erythrocyte Lysis Buffer to remove blood cells and the solution was then spun at 0.5 × g for 5 min.

Xenograft Assay:

Article Title: Stearoyl-CoA desaturase-1 is a key factor for lung cancer-initiating cells
Article Snippet: .. In vivo studies For xenograft assay, single-cell suspension was obtained by enzymatic digestion of the spheroids using different cell numbers in a volume of 100 μ l of a 50% GFR Matrigel (BD Biosciences, Franklin Lakes, NJ, USA). .. In particular, Pe o/11 and NCI-H460 spheroids generated for 48 h in the absence or in the presence of SCD1 inhibitor (500 nM) were injected subcutaneously into the right flank of ten CD1 nude mice for each group.

Isolation:

Article Title: Tumor Necrosis Factor Disrupts Claudin-5 Endothelial Tight Junction Barriers in Two Distinct NF-κB-Dependent Phases
Article Snippet: .. HDMECs resident in the papillary dermis were isolated by dermatome slicing the superficial most 0.5 to 0.7 mm of skin, followed by fine mincing and enzymatic digestion (Dispase, 50 U/ml; BD Biosciences) at 37°C until the epidermis removed easily from the underlying dermis. .. Released dermal cells were cultured on 10 μg/ml human plasma fibronectin-coated (Millipore) tissue culture plastic (BD Biosciences) in EGM2-MV growth medium (Lonza) that was replaced at 48 hour intervals.

Centrifugation:

Article Title: Effect of red light and near infrared laser on the generation of reactive oxygen species in primary dermal fibroblasts
Article Snippet: .. Briefly, donated tissue was subjected to enzymatic digestion with collagenase solution, followed by high-speed centrifugation and filtration of the infranatant through a 40 μm filter (BD Biosciences, 352,340) to obtain the stromal vascular fraction (SVF) containing high numbers of blood cells with traces of endothelial cells, fibroblasts, pericytes and stem cells. .. Cell pellet containing the SVF was re-suspended in Erythrocyte Lysis Buffer to remove blood cells and the solution was then spun at 0.5 × g for 5 min.

Mouse Assay:

Article Title: Neurofibromin (Nf1) is required for skeletal muscle development
Article Snippet: .. Bulk muscle tissue from muscle above the abdomen, including lattisimus and diaprhagm, was excised from adult mice and subjected to enzymatic digestion with dispase (BD) and collagenase (Worthington) for 1h. .. The resulting suspension was consecutively filtered over 100 and 40 µm cell strainers and subsequently subjected to fractionation using a discontinuous Percoll density gradient.

Staining:

Article Title: Binding of HMGN proteins to cell specific enhancers stabilizes cell identity
Article Snippet: .. Cells were removed with enzymatic digestion and single cell suspension were stained with antibodies specific for SSEA1 conjugated to PerCP-Cy (BD PharmingenTM, Cat# 561560) and EpCam conjugated to PE (Biolegend, Cat# 118206). .. Oct4-GFP-expressing MEFs were directly analyzed for GFP fluorescence.

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    Becton Dickinson enzymatic digestion
    Enzymatic Digestion, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymatic digestion/product/Becton Dickinson
    Average 93 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    enzymatic digestion - by Bioz Stars, 2020-08
    93/100 stars
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