Structured Review

Active Motif enzymatic digestion
Enzymatic Digestion, supplied by Active Motif, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/enzymatic digestion/product/Active Motif
Average 92 stars, based on 7 article reviews
Price from $9.99 to $1999.99
enzymatic digestion - by Bioz Stars, 2020-08
92/100 stars

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Related Articles

Sonication:

Article Title: Allele-specific quantitative proteomics unravels molecular mechanisms modulated by cis-regulatory PPARG locus variation
Article Snippet: .. Briefly, after enzymatic digestion for 15 min, 10 mM EDTA was added and chromatin was sheared using the EpiShear™ Probe Sonicator (Active Motif, La Hulpe, Belgium; 20 pulses consisting of 20 s sonication followed by 30 s rest at 25% amplitude) in the same buffer. .. Chromatin was then incubated for 30 min with protein G magnetic beads (Active Motif, La Hulpe, Belgium) and 2 μg of rabbit polyclonal αYY1 antibody (sc-281x, Santa Cruz Biotechnology, Heidelberg, Germany).

Article Title: The transcriptional coactivator Bob1 promotes the development of follicular T helper cells via Bcl6
Article Snippet: .. Briefly, after enzymatic digestion for 15 min, the chromatin was sheared using the EpiShearTM Probe Sonicator (Active Motif; 30 pulses consisting of 20 s sonication followed by 30 s rest at 25% amplitude) in the same buffer. .. The chromatin was precleared for 2 h with protein G microbeads (Invitrogen) and then incubated overnight with 2 μg of either rabbit polyclonal Oct1 (sc‐232), Oct2 (sc‐233), and Bob1 (sc‐955) antibodies (Santa Cruz Biotechnology).

Chromatin Immunoprecipitation:

Article Title: Transcriptional regulation of SP-B gene expression by nitric oxide in H441 lung epithelial cells
Article Snippet: .. Cell fixation, enzymatic digestion of DNA, and immunoprecipitation reactions were performed using the Chip-IT express enzymatic kit (Active Motif) according to the manufacturer's instructions. .. Normal IgG and TTF-1 (mouse monoclonal antibody, Thermo Scientific), Sp1 (Active Motif), and HNF-3α (FoxA1; Santa Cruz Biotechnology) antibodies were used at 2 μg/reaction in immunoprecipitation reactions.

Immunoprecipitation:

Article Title: Transcriptional regulation of SP-B gene expression by nitric oxide in H441 lung epithelial cells
Article Snippet: .. Cell fixation, enzymatic digestion of DNA, and immunoprecipitation reactions were performed using the Chip-IT express enzymatic kit (Active Motif) according to the manufacturer's instructions. .. Normal IgG and TTF-1 (mouse monoclonal antibody, Thermo Scientific), Sp1 (Active Motif), and HNF-3α (FoxA1; Santa Cruz Biotechnology) antibodies were used at 2 μg/reaction in immunoprecipitation reactions.

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    Active Motif enzymatic digestion
    Enzymatic Digestion, supplied by Active Motif, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymatic digestion/product/Active Motif
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    enzymatic digestion - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

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