Structured Review

Accumax enzymatic digestion
Enzymatic Digestion, supplied by Accumax, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/enzymatic digestion/product/Accumax
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
enzymatic digestion - by Bioz Stars, 2020-08
92/100 stars

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Related Articles

Flow Cytometry:

Article Title: Single-Cell RNA Sequencing-Based Computational Analysis to Describe Disease Heterogeneity
Article Snippet: .. For GSE81547 , the experimental models and human pancreas or islet samples were conducted in accordance with guidelines; during flow cytometry, isolated human islets were dissociated into single cells by enzymatic digestion using Accumax (Invitrogen). .. Next, single-cell RNA-seq libraries were generated as described in the literature, and barcoded libraries were pooled and subjected to 75 bp paired-end sequencing on the Illumina NextSeq instrument.

Isolation:

Article Title: Single-Cell RNA Sequencing-Based Computational Analysis to Describe Disease Heterogeneity
Article Snippet: .. For GSE81547 , the experimental models and human pancreas or islet samples were conducted in accordance with guidelines; during flow cytometry, isolated human islets were dissociated into single cells by enzymatic digestion using Accumax (Invitrogen). .. Next, single-cell RNA-seq libraries were generated as described in the literature, and barcoded libraries were pooled and subjected to 75 bp paired-end sequencing on the Illumina NextSeq instrument.

Transferring:

Article Title: Pten Deletion in Adult Neural Stem/Progenitor Cells Enhances Constitutive Neurogenesis
Article Snippet: .. Briefly, 8 week old SEZs were dissected, minced with a razor blade, dissociated by enzymatic digestion with Accumax followed by light trituration with a fire-polished glass pipette, and passed through a 70 µM nylon mesh. .. For moderate density cultures, cells were re-suspended at 10,000 cells/ml (10K) in Neurobasal medium (Gibco), supplemented with B-27 (Gibco), Heparin (Sigma), 20 ng/ml basic fibroblast growth factor (bFGF, Peprotech), and 20 ng/ml epidermal growth factor (EGF, Invitrogen).

Cytometry:

Article Title: Single-Cell RNA Sequencing-Based Computational Analysis to Describe Disease Heterogeneity
Article Snippet: .. For GSE81547 , the experimental models and human pancreas or islet samples were conducted in accordance with guidelines; during flow cytometry, isolated human islets were dissociated into single cells by enzymatic digestion using Accumax (Invitrogen). .. Next, single-cell RNA-seq libraries were generated as described in the literature, and barcoded libraries were pooled and subjected to 75 bp paired-end sequencing on the Illumina NextSeq instrument.

other:

Article Title: Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
Article Snippet: Cell aggregates were dissociated by enzymatic digestion in Accumax (AM105/Funakoshi), Y-27632 and DNase I for 10 min at 37 °C, followed by mechanical trituration, pelleting and plated at a density of 200,000–300,000 cells/ml/well in dissociation medium, which contained DFNB, 10% FBS (SFBM30-2537/Equitech-Bio), BDNF (028-16451/WAKO), NT-3 (146-09231/WAKO) and LM22A-4 (SML0848/SIGMA), with 10 μM Y-27632.

Staining:

Article Title: Discovering human diabetes-risk gene function with genetics and physiological assays
Article Snippet: .. Intracellular staining and FACS sorting of human islet cells Pseudo islets were dispersed into single cells by brief enzymatic digestion (Accumax, Invitrogen). ..

FACS:

Article Title: Discovering human diabetes-risk gene function with genetics and physiological assays
Article Snippet: .. Intracellular staining and FACS sorting of human islet cells Pseudo islets were dispersed into single cells by brief enzymatic digestion (Accumax, Invitrogen). ..

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    Accumax enzymatic digestion
    Enzymatic Digestion, supplied by Accumax, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymatic digestion/product/Accumax
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    enzymatic digestion - by Bioz Stars, 2020-08
    92/100 stars
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