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ATUM enzymatic digestion
Enzymatic Digestion, supplied by ATUM, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/enzymatic digestion/product/ATUM
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
enzymatic digestion - by Bioz Stars, 2020-08
92/100 stars

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Article Title: Biomonitoring of Aristolactam-DNA Adducts in Human Tissues using Ultra-Performance Liquid Chromatography/Ion-Trap Mass Spectrometry
Article Snippet: .. The enzymatic digestion conditions used for the hydrolysis of DNA (20 μ g) to 2′-deoxynucleosides have been described previously and employed DNase I for 1.5 h, followed by incubation with nuclease P1 for 3 h, and lastly by incubation with alkaline phosphatase and spleen phosphodiesterase for 18 h at 37 °C. .. We have shown that these conditions are highly efficient in the enzymatic digestion of DNA modified with bulky carcinogens of diverse structures., Isotopically labeled internal standards were added to the DNA prior to enzymatic digestion at a level of 5 adducts per 108 DNA bases for genomic mouse DNA, human renal cortex DNA, and DNA from human urothelial tumor tissue.