enzymatic digestion buffers  (Roche)


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    Structured Review

    Roche enzymatic digestion buffers
    Enzymatic Digestion Buffers, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymatic digestion buffers/product/Roche
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    enzymatic digestion buffers - by Bioz Stars, 2020-09
    85/100 stars

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    Related Articles

    Incubation:

    Article Title: Exposure to Candida albicans Polarizes a T-Cell Driven Arthritis Model towards Th17 Responses, Resulting in a More Destructive Arthritis
    Article Snippet: .. In short, synovial biopsies were incubated with enzymatic digestion buffers (Liberase Blendzyme, Roche) for 30 minutes at 37°C. .. Next, a 70 µm nylon cell strainer (BD Falcon) was used to process the digested tissue.

    other:

    Article Title: Treatment of collagenase-induced osteoarthritis with a viral vector encoding TSG-6 results in ectopic bone formation
    Article Snippet: Synovial biopsies in Trizol were first homogenated using Magnalyzer Green Beads (Roche Life Sciences, Almere, The Netherlands) and processed according to the provided protocol.

    RNA Sequencing Assay:

    Article Title: Functionally distinct disease-associated fibroblast subsets in rheumatoid arthritis
    Article Snippet: .. We repeated these procedures every 15 min for total 1 h. For the analysis of synovial biopsy samples, we digested the tissues with 0.05 mg/mL Liberase TM (Roche) and 0.04 mg/mL DNaseI at 37 °C for 30 min. For single-cell RNA-seq, we digested tissues with 0.2 mg/mL Liberase TL (Roche), 0.1 mg/mL DNaseI in RPMI at 37 °C for 20 min to minimize the cleavage of surface markers of lymphocytes during the enzymatic digestion. .. After lysing red blood cells with ACK-lysing buffer, we stained cells with antibodies, and sorted by FACSAria Fusion (BD) with 100 μm nozzle at 20 psi.

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  • 85
    Roche enzymatic digestion buffers
    Enzymatic Digestion Buffers, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymatic digestion buffers/product/Roche
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    enzymatic digestion buffers - by Bioz Stars, 2020-09
    85/100 stars
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