Journal: Cell host & microbe

Article Title: Legionella-infected macrophages engage the alveolar epithelium to metabolically reprogram myeloid cells and promote antibacterial inflammation

doi: 10.1016/j.chom.2020.07.019

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Collagenase IV , Worthington Biochemical , Cat# {"type":"entrez-nucleotide","attrs":{"text":"LS004188","term_id":"1321650536","term_text":"LS004188"}} LS004188.

Techniques: Recombinant, Isolation, Enzyme-linked Immunosorbent Assay, Plex Assay, Flow Cytometry, Software

Journal: American Journal of Cancer Research

Article Title: SHMT2 promotes tumor growth through VEGF and MAPK signaling pathway in breast cancer

doi:

Figure Lengend Snippet: SHMT2 promotes breast cancer cell proliferation by activating MAPK pathway. Western blotting analysis of proteins with phosphorylation or not related in MAPK signaling pathway in breast cancer cells with SHMT2 knockdown (A) and over-expression (B). Cell viability was analyzed by MTT assay in HCC1806 cells with SHMT2 knockdown treated with an ERK inhibitor FR180204 (C) or a p38 inhibitor SB202190 (D). ZR-75-1 cells with SHMT2 overexpression were treated with an ERK inhibitor FR180204 (E) or a p38 inhibitor SB202190 (F). # represents the comparison between inhibitor plus SHMT2 over-expression group and SHMT2 over-expression group. * represents the comparison between treatment group and control group. #, *P < 0.05. Student’s t-test. The data are presented as the mean ± SD. The error bars show the SD.

Article Snippet: ERK MAPK inhibitor {"type":"entrez-nucleotide","attrs":{"text":"FR180204","term_id":"258307209","term_text":"FR180204"}} FR180204 (HY-12275), p38 MAPK inhibitor SB202190 (HY-10295) were purchased from the Med Chem Express (MCE).

Techniques: Western Blot, Over Expression, MTT Assay

Journal: International Journal of Ophthalmology

Article Title: Vascular endothelial growth factor-165b protects the blood-retinal barrier from damage after acute high intraocular pressure in rats

doi: 10.18240/ijo.2022.08.02

Figure Lengend Snippet: The antibodies used in this study

Article Snippet: Retinal sections were observed by confocal microscopy (Olympus FV1000, Japan). table ft1 table-wrap mode="anchored" t5 caption a7 Product Host Company Catalogue number Dilution Anti-VEGF 165b antibody[MRVL56/1] Mouse Abcam ab14994 2 µg/mL for WB; 5 µg/mL for IF Anti-CD31 antibody[EPR17260-263] Rabbit Abcam ab222783 1:100 IF Anti-CD31 antibody[P2B1] Mouse Abcam Ab24590 2 µg/mL for IF Anti-NeuN antibody Rabbit Abcam ab104225 1:500 IF Anti-ZO-1 antibody Rabbit Abcam ab96587 1:1000 WB; 1:500 IF Alexa Fluor 488 anti-rabbit IgG(H+L) Donkey Jackson 711-545-152 1:200 IF Alexa Fluor ® 594 anti-mouseIgG(H+L) Donkey Jackson 715-585-150 1:200 IF Alexa Fluor ® 488 anti-mouse IgG(H+L) Donkey Jackson 715-545-150 1:200 IF Alexa Fluor ® 594 anti-rabbit IgG(H+L) Donkey Jackson 711-585-152 1:200 IF Anti-β-actin loading control Rabbit Abcam Ab1801 1:2000 WB HRP-conjugated anti-mouse IgG(H+L) Goat Abcam {"type":"entrez-nucleotide","attrs":{"text":"Ab205719","term_id":"73912114","term_text":"AB205719"}} Ab205719 1:10000 WB HRP-conjugated anti-rabbit IgG(H+L) Goat Abcam {"type":"entrez-nucleotide","attrs":{"text":"Ab205718","term_id":"73912113","term_text":"AB205718"}} Ab205718 1:10000 WB Open in a separate window WB: Western blotting; IF: Immunofluorescence. caption a8 The antibodies used in this study

Techniques: