Journal: Veterinary Sciences

Article Title: Isolation and Characterization of Vancomycin-Resistant Enterococcus faecium from Cattle: Antimicrobial Resistance, Virulence Genes, and Pathogenicity

doi: 10.3390/vetsci12090880

Figure Lengend Snippet: Identification and molecular characterization of E. faecium isolates. ( A ) Colony morphology on Enterococcus-selective agar showing brown-black colonies with brown halos. ( B ) Colony morphology on blood agar with small, round, slightly raised, grayish-white, translucent colonies. ( C ) Gram staining showing Gram-positive cocci, occurring singly or in pairs. ( D ) PCR amplification of the ddl gene showing a 176 bp product specific to E. faecium ; Lanes 1–3 in correspond to isolates SCQ3, SCQ4, and SCQ11, respectively. ( E ) 16S rRNA gene amplification producing an approximately 1500 bp band; Lanes 1–3 in correspond to isolates to SCQ3, SCQ4, and SCQ11, respectively. ( F ) Neighbor-joining phylogenetic tree constructed using MEGA X from aligned 16S rRNA sequences, illustrating genetic relationships between isolates and reference E. faecium strains from NCBI. Bootstrap values (n = 1000 replicates) shown at branches; scale bar indicates evolutionary distance.

Article Snippet: Cultures were then streaked onto Pfizer enterococcus selective agar plates (Pfizer Inc., Shanghai, China) and incubated at 37 °C for 18–24 h. Colonies appeared as brown/black with brown halos, and the colony morphology was observed and recorded.

Techniques: Staining, Amplification, Construct

Journal: bioRxiv

Article Title: LicD-Mediated Cell Wall Decoration Governs Phage Sensitivity in Enterococcus faecalis Clinical Isolates

doi: 10.1101/2025.05.13.653012

Figure Lengend Snippet: Antibiofilm activity of Enterococcus phages . (a) Minimum biofilm eradication concentration presented as percent reduction in the biofilm biomass compared to non-treated control using phage titres ranging from (9-5) log 10 PFU/mL. (b) antibiofilm activity of Enterococcus phages against host ATCC-700802 and clinical isolates 8010 and 8050. Data presented as mean ± SD n=3. ns: non-significant, p > 0.05, *p < 0.05; **p < 0.01, ***p < 0.001 one-way ANOVA followed by multiple comparisons tests.

Article Snippet: Briefly, 100 µL of the collected samples were spread on Difco™ m Enterococcus selective Agar (Thermo Fisher Scientific Australia Pty Ltd., Scoresby, VIC, Australia) and incubated at 37°C.

Techniques: Activity Assay, Concentration Assay, Control

Journal: bioRxiv

Article Title: LicD-Mediated Cell Wall Decoration Governs Phage Sensitivity in Enterococcus faecalis Clinical Isolates

doi: 10.1101/2025.05.13.653012

Figure Lengend Snippet: Time-kill kinetics of Enterococcus bacteriophages. (a) Growth pattern of E. faecalis ATTC 700802 incubated with individual phages APTC-Efa.10, Efa.16 and Efa.20 (MOI) in the range between (0.01-100). (b) Growth pattern of E. faecalis ATTC 700802 incubated with 1:1 phage cocktail at different MOIs (0.01-100).

Article Snippet: Briefly, 100 µL of the collected samples were spread on Difco™ m Enterococcus selective Agar (Thermo Fisher Scientific Australia Pty Ltd., Scoresby, VIC, Australia) and incubated at 37°C.

Techniques: Incubation