enhanced chemiluminescence prime reagent  (Millipore)


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    Name:
    ECL Prime Western Blotting Detection Reagent
    Description:
    Amersham ECL Prime is a highly sensitive reagent characterized by stable signal emission allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run The high intensity of the emitted signals means that proteins can be detected using highly diluted primary and secondary antibodies contributing to low background and reducing consumption of reagents Amersham ECL Prime consolidates and builds on the benefits of Amersham ECL Plus and Amersham ECL Advance to deliver a detection system that is sensitive stable precisely quantitative across a wide dynamic range of protein levels and conservative in its consumption of expensive antibody reagents
    Catalog Number:
    gerpn2236
    Price:
    None
    Applications:
    ECL(TM) Prime Western blotting detection reagent has been used to detect chemiluminescent signals during Western blotting.
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    Structured Review

    Millipore enhanced chemiluminescence prime reagent
    Amersham ECL Prime is a highly sensitive reagent characterized by stable signal emission allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run The high intensity of the emitted signals means that proteins can be detected using highly diluted primary and secondary antibodies contributing to low background and reducing consumption of reagents Amersham ECL Prime consolidates and builds on the benefits of Amersham ECL Plus and Amersham ECL Advance to deliver a detection system that is sensitive stable precisely quantitative across a wide dynamic range of protein levels and conservative in its consumption of expensive antibody reagents
    https://www.bioz.com/result/enhanced chemiluminescence prime reagent/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    enhanced chemiluminescence prime reagent - by Bioz Stars, 2020-07
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Trans-Fats Inhibit Autophagy Induced by Saturated Fatty Acids
    Article Snippet: .. Immunoreactive bands were visualized with ECL prime western blotting detection reagent (Sigma-Aldrich) by means of an ImageQuant LAS4000 (GE Healthcare, Little Chalfont, UK). ..

    Article Title: 3,4‐Dimethoxychalcone induces autophagy through activation of the transcription factors TFE3 and TFEB
    Article Snippet: .. Immunoreactive bands were visualized with ECL prime Western blotting detection reagent (Sigma‐Aldrich). .. Nuclear fractionation was performed with the nuclear extraction kit (ab113474, Abcam) according to the manufacturer's advice, followed by immunoblotting as described above.

    Article Title: Effect of AMPK signal pathway on pathogenesis of abdominal aortic aneurysms
    Article Snippet: .. Blots were detected using an ECL Prime Western Blotting Detection reagent (Millipore). .. The signals were quantified by ImageJ software.

    Article Title: Liposome co-incubation with cancer cells secreted exosomes (extracellular vesicles) with different proteins expressions and different uptake pathways
    Article Snippet: .. The membrane was visualized by incubation with Amersham™ ECL™ Prime Western Blotting Detection Reagent (Sigma Aldrich, MO, US) for 5 min at room temperature then imaged using image quant LAS 4000 (GE Healthcare Bio-Sciences, MA, US). .. Exosome (EV) marker, TSG101, was used as control (housekeeping) antigens.

    Article Title: Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa
    Article Snippet: .. 2.6.4 Imaging and normalization of protein bands The Amersham ECL Prime Western blotting detection reagent (Sigma-Aldrich, Germany) containing a chemiluminescent substrate was applied to the blot according to the manufacturer’s recommendations. .. To control for variability during the protein loading step in the Western blot, β-actin was used as a loading control (LC) for normalization of the CatSper2 and Tektin2 proteins.

    Article Title: Novel tetrahydroacridine derivatives with iodobenzoic moieties induce G0/G1 cell cycle arrest and apoptosis in A549 non-small lung cancer and HT-29 colorectal cancer cells
    Article Snippet: .. Protein bands were visualized using ECL™ Prime Western Blotting Detection Reagents (Sigma Aldrich) Healthcare and Carestream Kodak autoradiography GBX fixer/replenisher (Sigma Aldrich) with Developer and Replenisher (Carestream) in the dark room. .. Results were obtained on Autoradiography Film (Santa Cruz Biotechnology).

    Article Title: Dosage analysis of the 7q11.23 Williams region identifies BAZ1B as a major human gene patterning the modern human face and underlying self-domestication
    Article Snippet: .. Blots were detected with the ECL Prime Western Blotting Detection Reagents (Sigma-Aldrich) and scanned using the ChemiDoc system (Bio-Rad). .. Wound-healing assay Cells (5 × 104 to 7 × 104 ) were plated in each of the two Matrigel-coated wells of silicone culture-inserts (Ibidi) attached to six-well culture plates.

    Incubation:

    Article Title: Liposome co-incubation with cancer cells secreted exosomes (extracellular vesicles) with different proteins expressions and different uptake pathways
    Article Snippet: .. The membrane was visualized by incubation with Amersham™ ECL™ Prime Western Blotting Detection Reagent (Sigma Aldrich, MO, US) for 5 min at room temperature then imaged using image quant LAS 4000 (GE Healthcare Bio-Sciences, MA, US). .. Exosome (EV) marker, TSG101, was used as control (housekeeping) antigens.

    Autoradiography:

    Article Title: Novel tetrahydroacridine derivatives with iodobenzoic moieties induce G0/G1 cell cycle arrest and apoptosis in A549 non-small lung cancer and HT-29 colorectal cancer cells
    Article Snippet: .. Protein bands were visualized using ECL™ Prime Western Blotting Detection Reagents (Sigma Aldrich) Healthcare and Carestream Kodak autoradiography GBX fixer/replenisher (Sigma Aldrich) with Developer and Replenisher (Carestream) in the dark room. .. Results were obtained on Autoradiography Film (Santa Cruz Biotechnology).

    Imaging:

    Article Title: Influence of cryopreservation on the CATSPER2 and TEKT2 expression levels and protein levels in human spermatozoa
    Article Snippet: .. 2.6.4 Imaging and normalization of protein bands The Amersham ECL Prime Western blotting detection reagent (Sigma-Aldrich, Germany) containing a chemiluminescent substrate was applied to the blot according to the manufacturer’s recommendations. .. To control for variability during the protein loading step in the Western blot, β-actin was used as a loading control (LC) for normalization of the CatSper2 and Tektin2 proteins.

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