engen lba cas12a cpf1  (New England Biolabs)


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    New England Biolabs engen lba cas12a cpf1
    One-tube nested <t>PCR/CRISPR-Cas12a</t> assay schematic. (A) MBs-based viral RNA extraction and (B) assay with visual (fluorescence or naked eye) readout.
    Engen Lba Cas12a Cpf1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/engen lba cas12a cpf1/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    engen lba cas12a cpf1 - by Bioz Stars, 2024-10
    86/100 stars

    Images

    1) Product Images from "One-Tube Nested PCR Coupled with CRISPR-Cas12a for Ultrasensitive Nucleic Acid Testing"

    Article Title: One-Tube Nested PCR Coupled with CRISPR-Cas12a for Ultrasensitive Nucleic Acid Testing

    Journal: ACS Omega

    doi: 10.1021/acsomega.4c03911

    One-tube nested PCR/CRISPR-Cas12a assay schematic. (A) MBs-based viral RNA extraction and (B) assay with visual (fluorescence or naked eye) readout.
    Figure Legend Snippet: One-tube nested PCR/CRISPR-Cas12a assay schematic. (A) MBs-based viral RNA extraction and (B) assay with visual (fluorescence or naked eye) readout.

    Techniques Used: Nested PCR, CRISPR, RNA Extraction, Fluorescence

    CRISPR Cas12a detection optimization. (A) ssDNA reporter effects, (B) UV end point images, (C) natural light detection, (D) Cas12a concentration effects, and (E) Cas12a/crRNA ratio effects.
    Figure Legend Snippet: CRISPR Cas12a detection optimization. (A) ssDNA reporter effects, (B) UV end point images, (C) natural light detection, (D) Cas12a concentration effects, and (E) Cas12a/crRNA ratio effects.

    Techniques Used: CRISPR, Concentration Assay

    LOD for nested PCR/CRISPR-Cas12a assay. (A) RdRp region linearity curve, (B) N gene linearity curve, (C) RdRp region LOD, and (D) N gene LOD.
    Figure Legend Snippet: LOD for nested PCR/CRISPR-Cas12a assay. (A) RdRp region linearity curve, (B) N gene linearity curve, (C) RdRp region LOD, and (D) N gene LOD.

    Techniques Used: Nested PCR, CRISPR

    Real-time fluorescence results for nested PCR/CRISPR-Cas12a assay on clinical specimens.
    Figure Legend Snippet: Real-time fluorescence results for nested PCR/CRISPR-Cas12a assay on clinical specimens.

    Techniques Used: Fluorescence, Nested PCR, CRISPR

    Concordance analysis between the one-tube nested PCR/CRISPR-Cas12a assay and the RT-qPCR assay.
    Figure Legend Snippet: Concordance analysis between the one-tube nested PCR/CRISPR-Cas12a assay and the RT-qPCR assay.

    Techniques Used: Nested PCR, CRISPR, Quantitative RT-PCR

    engen lba cas12a  (New England Biolabs)


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    New England Biolabs engen lba cas12a
    One-tube nested <t>PCR/CRISPR-Cas12a</t> assay schematic. (A) MBs-based viral RNA extraction and (B) assay with visual (fluorescence or naked eye) readout.
    Engen Lba Cas12a, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/engen lba cas12a/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    engen lba cas12a - by Bioz Stars, 2024-10
    86/100 stars

    Images

    1) Product Images from "One-Tube Nested PCR Coupled with CRISPR-Cas12a for Ultrasensitive Nucleic Acid Testing"

    Article Title: One-Tube Nested PCR Coupled with CRISPR-Cas12a for Ultrasensitive Nucleic Acid Testing

    Journal: ACS Omega

    doi: 10.1021/acsomega.4c03911

    One-tube nested PCR/CRISPR-Cas12a assay schematic. (A) MBs-based viral RNA extraction and (B) assay with visual (fluorescence or naked eye) readout.
    Figure Legend Snippet: One-tube nested PCR/CRISPR-Cas12a assay schematic. (A) MBs-based viral RNA extraction and (B) assay with visual (fluorescence or naked eye) readout.

    Techniques Used: Nested PCR, CRISPR, RNA Extraction, Fluorescence

    CRISPR Cas12a detection optimization. (A) ssDNA reporter effects, (B) UV end point images, (C) natural light detection, (D) Cas12a concentration effects, and (E) Cas12a/crRNA ratio effects.
    Figure Legend Snippet: CRISPR Cas12a detection optimization. (A) ssDNA reporter effects, (B) UV end point images, (C) natural light detection, (D) Cas12a concentration effects, and (E) Cas12a/crRNA ratio effects.

    Techniques Used: CRISPR, Concentration Assay

    LOD for nested PCR/CRISPR-Cas12a assay. (A) RdRp region linearity curve, (B) N gene linearity curve, (C) RdRp region LOD, and (D) N gene LOD.
    Figure Legend Snippet: LOD for nested PCR/CRISPR-Cas12a assay. (A) RdRp region linearity curve, (B) N gene linearity curve, (C) RdRp region LOD, and (D) N gene LOD.

    Techniques Used: Nested PCR, CRISPR

    Real-time fluorescence results for nested PCR/CRISPR-Cas12a assay on clinical specimens.
    Figure Legend Snippet: Real-time fluorescence results for nested PCR/CRISPR-Cas12a assay on clinical specimens.

    Techniques Used: Fluorescence, Nested PCR, CRISPR

    Concordance analysis between the one-tube nested PCR/CRISPR-Cas12a assay and the RT-qPCR assay.
    Figure Legend Snippet: Concordance analysis between the one-tube nested PCR/CRISPR-Cas12a assay and the RT-qPCR assay.

    Techniques Used: Nested PCR, CRISPR, Quantitative RT-PCR

    engen lba cas12a cpf1  (New England Biolabs)


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    New England Biolabs engen lba cas12a cpf1
    Engen Lba Cas12a Cpf1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/engen lba cas12a cpf1/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    engen lba cas12a cpf1 - by Bioz Stars, 2024-10
    86/100 stars

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    engen lba cas12a cpf1  (New England Biolabs)


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    New England Biolabs engen lba cas12a cpf1
    Engen Lba Cas12a Cpf1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/engen lba cas12a cpf1/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    engen lba cas12a  (New England Biolabs)


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    New England Biolabs engen lba cas12a
    A , PCR evaluation of the specificity of Foc TR4-specific primers . B , In vitro cleavage of Foc TR4-specific PCR products from SCAR region by Lba <t>Cas12a</t> and crRNAs. C , Visual representation of lateral flow strips resulting from the SCAR region DETECTR assay. D , PCR evaluation of the specificity of Foc TR4-specific primers . E , In vitro cleavage of Foc TR4-specific PCR products from SeqA region by Lba Cas12a and crRNAs. F , Visual representation of lateral flow strips resulting from the SeqA region DETECTR assay.
    Engen Lba Cas12a, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/engen lba cas12a/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    engen lba cas12a - by Bioz Stars, 2024-10
    86/100 stars

    Images

    1) Product Images from "Rapid and Sensitive Detection of Fusarium oxysporum f. sp. cubense Tropical Race 4 Using a RPA-DETECTR Assay"

    Article Title: Rapid and Sensitive Detection of Fusarium oxysporum f. sp. cubense Tropical Race 4 Using a RPA-DETECTR Assay

    Journal: bioRxiv

    doi: 10.1101/2024.08.15.608054

    A , PCR evaluation of the specificity of Foc TR4-specific primers . B , In vitro cleavage of Foc TR4-specific PCR products from SCAR region by Lba Cas12a and crRNAs. C , Visual representation of lateral flow strips resulting from the SCAR region DETECTR assay. D , PCR evaluation of the specificity of Foc TR4-specific primers . E , In vitro cleavage of Foc TR4-specific PCR products from SeqA region by Lba Cas12a and crRNAs. F , Visual representation of lateral flow strips resulting from the SeqA region DETECTR assay.
    Figure Legend Snippet: A , PCR evaluation of the specificity of Foc TR4-specific primers . B , In vitro cleavage of Foc TR4-specific PCR products from SCAR region by Lba Cas12a and crRNAs. C , Visual representation of lateral flow strips resulting from the SCAR region DETECTR assay. D , PCR evaluation of the specificity of Foc TR4-specific primers . E , In vitro cleavage of Foc TR4-specific PCR products from SeqA region by Lba Cas12a and crRNAs. F , Visual representation of lateral flow strips resulting from the SeqA region DETECTR assay.

    Techniques Used: In Vitro

    engen lba cas12a cpf1  (New England Biolabs)


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    New England Biolabs engen lba cas12a cpf1
    Schematic light-activated deprotection of a reaction module coupled to the <t>CRISPR-Cas12a</t> (LAC12a) machinery for the amplified miRNA detection in cells.
    Engen Lba Cas12a Cpf1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/engen lba cas12a cpf1/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    engen lba cas12a cpf1 - by Bioz Stars, 2024-10
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    1) Product Images from "Light-activated CRISPR-Cas12a for amplified imaging of microRNA in cell cycle phases at single-cell levels"

    Article Title: Light-activated CRISPR-Cas12a for amplified imaging of microRNA in cell cycle phases at single-cell levels

    Journal: Science Advances

    doi: 10.1126/sciadv.adp6166

    Schematic light-activated deprotection of a reaction module coupled to the CRISPR-Cas12a (LAC12a) machinery for the amplified miRNA detection in cells.
    Figure Legend Snippet: Schematic light-activated deprotection of a reaction module coupled to the CRISPR-Cas12a (LAC12a) machinery for the amplified miRNA detection in cells.

    Techniques Used: CRISPR, Amplification

    engen lba cas12a  (New England Biolabs)


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    New England Biolabs engen lba cas12a
    a Streamlined onsite workflow. The assay executes the nucleic acid (NA) extraction and the CreDiT reaction in a single pot, minimizing the hands-on times. The total assay time is about 36 min. b CreDiT assay principle. Two reactions concurrently occur: recombinase polymerase amplification (RPA) and <t>Cas12a-based</t> signal generation. RPA is the major reaction that replicates the target DNA. During RPA, the polymerase displaces the DNA duplex, exposing single-stranded regions recognizable by Cas12a/gRNA complexes. This recognition event activates the complexes to cleave dye-quencher (F-Q) probes, generating fluorescent signals. As the RPA cycles proceed, more Cas12a/gRNA complexes are activated, amplifying the overall signal. All reactions are carried out at a constant temperature (42 °C) without requiring external interruptions. c A compact, integrated assay system was developed. The device had a turret-shaped heating block accommodating 12 samples and an optical module for fluorescent detection (LED light-emitting diode, PD photodiode). The heating block, mounted on a motor, rotated to position each sample for fluorescent measurements. The device had a small form factor (14 × 11 × 6.5 cm 3 ) and connected to a computer. Custom-designed software running on a computer presented a graphical user interface for device control and real-time data analysis. d Two microcontroller units (MCUs) automated the CreDiT assay processes. MCU1 controlled heating elements to maintain the desired temperatures and rotated the heating block during optical detection. MCU2 generated waveforms for fluorescent excitation and detected the resulting signal.
    Engen Lba Cas12a, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/engen lba cas12a/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    engen lba cas12a - by Bioz Stars, 2024-10
    86/100 stars

    Images

    1) Product Images from "Empowering the on-site detection of nucleic acids by integrating CRISPR and digital signal processing"

    Article Title: Empowering the on-site detection of nucleic acids by integrating CRISPR and digital signal processing

    Journal: Nature Communications

    doi: 10.1038/s41467-024-50588-3

    a Streamlined onsite workflow. The assay executes the nucleic acid (NA) extraction and the CreDiT reaction in a single pot, minimizing the hands-on times. The total assay time is about 36 min. b CreDiT assay principle. Two reactions concurrently occur: recombinase polymerase amplification (RPA) and Cas12a-based signal generation. RPA is the major reaction that replicates the target DNA. During RPA, the polymerase displaces the DNA duplex, exposing single-stranded regions recognizable by Cas12a/gRNA complexes. This recognition event activates the complexes to cleave dye-quencher (F-Q) probes, generating fluorescent signals. As the RPA cycles proceed, more Cas12a/gRNA complexes are activated, amplifying the overall signal. All reactions are carried out at a constant temperature (42 °C) without requiring external interruptions. c A compact, integrated assay system was developed. The device had a turret-shaped heating block accommodating 12 samples and an optical module for fluorescent detection (LED light-emitting diode, PD photodiode). The heating block, mounted on a motor, rotated to position each sample for fluorescent measurements. The device had a small form factor (14 × 11 × 6.5 cm 3 ) and connected to a computer. Custom-designed software running on a computer presented a graphical user interface for device control and real-time data analysis. d Two microcontroller units (MCUs) automated the CreDiT assay processes. MCU1 controlled heating elements to maintain the desired temperatures and rotated the heating block during optical detection. MCU2 generated waveforms for fluorescent excitation and detected the resulting signal.
    Figure Legend Snippet: a Streamlined onsite workflow. The assay executes the nucleic acid (NA) extraction and the CreDiT reaction in a single pot, minimizing the hands-on times. The total assay time is about 36 min. b CreDiT assay principle. Two reactions concurrently occur: recombinase polymerase amplification (RPA) and Cas12a-based signal generation. RPA is the major reaction that replicates the target DNA. During RPA, the polymerase displaces the DNA duplex, exposing single-stranded regions recognizable by Cas12a/gRNA complexes. This recognition event activates the complexes to cleave dye-quencher (F-Q) probes, generating fluorescent signals. As the RPA cycles proceed, more Cas12a/gRNA complexes are activated, amplifying the overall signal. All reactions are carried out at a constant temperature (42 °C) without requiring external interruptions. c A compact, integrated assay system was developed. The device had a turret-shaped heating block accommodating 12 samples and an optical module for fluorescent detection (LED light-emitting diode, PD photodiode). The heating block, mounted on a motor, rotated to position each sample for fluorescent measurements. The device had a small form factor (14 × 11 × 6.5 cm 3 ) and connected to a computer. Custom-designed software running on a computer presented a graphical user interface for device control and real-time data analysis. d Two microcontroller units (MCUs) automated the CreDiT assay processes. MCU1 controlled heating elements to maintain the desired temperatures and rotated the heating block during optical detection. MCU2 generated waveforms for fluorescent excitation and detected the resulting signal.

    Techniques Used: Extraction, Recombinase Polymerase Amplification, Blocking Assay, Software, Control, Generated

    a For a given target DNA (gray backbone), a pair of CreDiT probes (forward and reverse) were designed, with each probe consisting of an RPA primer (green backbone) and a Cas12a/gRNA complex (orange backbone). Upon binding to the target DNA, the primer exposes a downstream sequence complementary to gRNA. The Cas12a/gRNA complex then recognizes its target and becomes an active endonuclease to cleave F-Q reporters. HPV16 probes are shown. b Assay validation with varying reaction conditions. HPV16 DNA was used as a model target ([DNA] = 4 × 10 7 copies/mL). A high signal was only observed when all assay components were present. Data were displayed as mean ± s.d. from technical triplicates. c CreDiT (20 min) and qPCR (1 h) assays were used to analyze samples with different target DNA concentrations. The CReDiT assay exhibited a lower detection limit (40 copies/mL) and a wider dynamic range (4 orders of magnitude) than qPCR (detection limit, 400 copies/mL; dynamic range, 2.5 orders of magnitude). ∆CreDiT is the background-subtracted signal. Data were displayed as mean ± s.d. from technical triplicates. d Probe selectivity. A panel of probes was designed for hrHPV targets (HPV16, HPV18, HPV31, HPV33, HPV45, and HPV58) and GAPDH . These probes detected their intended targets with negligible off-target signals. [Target DNA] = 4 × 10 7 copies/mL. The heatmap displays mean values from technical triplicates. a.u. arbitrary unit. Source data are provided as a Source Data file.
    Figure Legend Snippet: a For a given target DNA (gray backbone), a pair of CreDiT probes (forward and reverse) were designed, with each probe consisting of an RPA primer (green backbone) and a Cas12a/gRNA complex (orange backbone). Upon binding to the target DNA, the primer exposes a downstream sequence complementary to gRNA. The Cas12a/gRNA complex then recognizes its target and becomes an active endonuclease to cleave F-Q reporters. HPV16 probes are shown. b Assay validation with varying reaction conditions. HPV16 DNA was used as a model target ([DNA] = 4 × 10 7 copies/mL). A high signal was only observed when all assay components were present. Data were displayed as mean ± s.d. from technical triplicates. c CreDiT (20 min) and qPCR (1 h) assays were used to analyze samples with different target DNA concentrations. The CReDiT assay exhibited a lower detection limit (40 copies/mL) and a wider dynamic range (4 orders of magnitude) than qPCR (detection limit, 400 copies/mL; dynamic range, 2.5 orders of magnitude). ∆CreDiT is the background-subtracted signal. Data were displayed as mean ± s.d. from technical triplicates. d Probe selectivity. A panel of probes was designed for hrHPV targets (HPV16, HPV18, HPV31, HPV33, HPV45, and HPV58) and GAPDH . These probes detected their intended targets with negligible off-target signals. [Target DNA] = 4 × 10 7 copies/mL. The heatmap displays mean values from technical triplicates. a.u. arbitrary unit. Source data are provided as a Source Data file.

    Techniques Used: Binding Assay, Sequencing

    engen lba cas12a  (New England Biolabs)


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    New England Biolabs engen lba cas12a
    Engen Lba Cas12a, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86/100 stars

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    engen lba cas12a cpf1  (New England Biolabs)


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    New England Biolabs engen lba cas12a cpf1
    Optimized experimental parameters of the <t>Cas12a</t> assay (A) Performance of different ssDNA reporters. (B) Assay time of the Cas12a system. (C) Amount of Cas12a and crRNA in a 30 μL reaction system. (D) Dose-response curves with crRNAs targeting different regions or strands. The error bars represent the standard deviation of the mean.
    Engen Lba Cas12a Cpf1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/engen lba cas12a cpf1/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    engen lba cas12a cpf1 - by Bioz Stars, 2024-10
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    Images

    1) Product Images from "An amplification-free CRISPR-Cas12a assay for titer determination and composition analysis of the rAAV genome"

    Article Title: An amplification-free CRISPR-Cas12a assay for titer determination and composition analysis of the rAAV genome

    Journal: Molecular Therapy. Methods & Clinical Development

    doi: 10.1016/j.omtm.2024.101304

    Optimized experimental parameters of the Cas12a assay (A) Performance of different ssDNA reporters. (B) Assay time of the Cas12a system. (C) Amount of Cas12a and crRNA in a 30 μL reaction system. (D) Dose-response curves with crRNAs targeting different regions or strands. The error bars represent the standard deviation of the mean.
    Figure Legend Snippet: Optimized experimental parameters of the Cas12a assay (A) Performance of different ssDNA reporters. (B) Assay time of the Cas12a system. (C) Amount of Cas12a and crRNA in a 30 μL reaction system. (D) Dose-response curves with crRNAs targeting different regions or strands. The error bars represent the standard deviation of the mean.

    Techniques Used: Standard Deviation

    Dose-response relationships of linearized plasmid, AAV5-IN, and AAV2 using the Cas12a assay Dotted lines represent 95% confidence intervals around the fitted lines. The error bars represent the standard deviation of the mean.
    Figure Legend Snippet: Dose-response relationships of linearized plasmid, AAV5-IN, and AAV2 using the Cas12a assay Dotted lines represent 95% confidence intervals around the fitted lines. The error bars represent the standard deviation of the mean.

    Techniques Used: Plasmid Preparation, Standard Deviation

    Performance evaluation of the developed Cas12a assay (A and B) Linear range for AAV5-IN genomic DNA. (C) Evaluation of the specificity using crRNAs carrying different point mutations. (D) Quantitative range and accuracy of the Cas12a assay. (E) Comparison of the estimated genome titers in the crGFP- and crWPRE-1-directed Cas12a assays. The error bars represent the standard deviation of the mean. “ns” indicates “no significant difference between two groups” ( p < 0.05, t test).
    Figure Legend Snippet: Performance evaluation of the developed Cas12a assay (A and B) Linear range for AAV5-IN genomic DNA. (C) Evaluation of the specificity using crRNAs carrying different point mutations. (D) Quantitative range and accuracy of the Cas12a assay. (E) Comparison of the estimated genome titers in the crGFP- and crWPRE-1-directed Cas12a assays. The error bars represent the standard deviation of the mean. “ns” indicates “no significant difference between two groups” ( p < 0.05, t test).

    Techniques Used: Comparison, Standard Deviation

    Intra- and inter-assay precision of the Cas12a assay
    Figure Legend Snippet: Intra- and inter-assay precision of the Cas12a assay

    Techniques Used: Inter Assay, Intra Assay

    Comparison of Cas12a, qPCR, and dPCR assay results (A) Results of Cas12a assay. (B) Results of qPCR assay. (C) Results of dPCR assay. (D) Summary of the estimated genome titers of denatured samples from the three assays. The error bars represent the standard deviation of the mean. “ns” indicates “no significant difference between Cas12a and qPCR,” and asterisks indicate the level of significance ( p < 0.05, t test).
    Figure Legend Snippet: Comparison of Cas12a, qPCR, and dPCR assay results (A) Results of Cas12a assay. (B) Results of qPCR assay. (C) Results of dPCR assay. (D) Summary of the estimated genome titers of denatured samples from the three assays. The error bars represent the standard deviation of the mean. “ns” indicates “no significant difference between Cas12a and qPCR,” and asterisks indicate the level of significance ( p < 0.05, t test).

    Techniques Used: Comparison, Standard Deviation

    Evaluation of Cas12a activity on ssDNA and dsDNA substrate (A) Distribution of crWPRE-1 and crWPRE-2. (B) Estimated copy numbers of samples comprising different proportions of positive (POS) strand and negative (NEG) strand using the Cas12a assay. The error bars represent the standard deviation of the mean.
    Figure Legend Snippet: Evaluation of Cas12a activity on ssDNA and dsDNA substrate (A) Distribution of crWPRE-1 and crWPRE-2. (B) Estimated copy numbers of samples comprising different proportions of positive (POS) strand and negative (NEG) strand using the Cas12a assay. The error bars represent the standard deviation of the mean.

    Techniques Used: Activity Assay, Standard Deviation

    Test results of Cas12a assay with crRNA targeting the positive-sense strand (crWPRE-1) and negative-sense strand (crWPRE-2) The error bars represent the standard deviation of the mean. “ns” indicates “no significant difference between two groups,” and asterisks indicate the level of significance ( p < 0.05, t test).
    Figure Legend Snippet: Test results of Cas12a assay with crRNA targeting the positive-sense strand (crWPRE-1) and negative-sense strand (crWPRE-2) The error bars represent the standard deviation of the mean. “ns” indicates “no significant difference between two groups,” and asterisks indicate the level of significance ( p < 0.05, t test).

    Techniques Used: Standard Deviation

    engen lba cas12a  (New England Biolabs)


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    New England Biolabs engen lba cas12a
    Engen Lba Cas12a, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    • engen lba cas12a cpf1  (New England Biolabs)
    86
    New England Biolabs engen lba cas12a cpf1
    One-tube nested <t>PCR/CRISPR-Cas12a</t> assay schematic. (A) MBs-based viral RNA extraction and (B) assay with visual (fluorescence or naked eye) readout.
    Engen Lba Cas12a Cpf1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/engen lba cas12a cpf1/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    engen lba cas12a cpf1 - by Bioz Stars, 2024-10
    86/100 stars
    		  Buy from Supplier
    engen lba cas12a  (New England Biolabs)
    86
    New England Biolabs engen lba cas12a
    One-tube nested <t>PCR/CRISPR-Cas12a</t> assay schematic. (A) MBs-based viral RNA extraction and (B) assay with visual (fluorescence or naked eye) readout.
    Engen Lba Cas12a, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/engen lba cas12a/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    engen lba cas12a - by Bioz Stars, 2024-10
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    One-tube nested PCR/CRISPR-Cas12a assay schematic. (A) MBs-based viral RNA extraction and (B) assay with visual (fluorescence or naked eye) readout.

    Journal: ACS Omega

    Article Title: One-Tube Nested PCR Coupled with CRISPR-Cas12a for Ultrasensitive Nucleic Acid Testing

    doi: 10.1021/acsomega.4c03911

    Figure Lengend Snippet: One-tube nested PCR/CRISPR-Cas12a assay schematic. (A) MBs-based viral RNA extraction and (B) assay with visual (fluorescence or naked eye) readout.

    Article Snippet: EnGen Lba Cas12a (Cpf1) and NEB buffer 2.1 were purchased from New England Biolabs (NEB, USA).

    Techniques: Nested PCR, CRISPR, RNA Extraction, Fluorescence

    CRISPR Cas12a detection optimization. (A) ssDNA reporter effects, (B) UV end point images, (C) natural light detection, (D) Cas12a concentration effects, and (E) Cas12a/crRNA ratio effects.

    Journal: ACS Omega

    Article Title: One-Tube Nested PCR Coupled with CRISPR-Cas12a for Ultrasensitive Nucleic Acid Testing

    doi: 10.1021/acsomega.4c03911

    Figure Lengend Snippet: CRISPR Cas12a detection optimization. (A) ssDNA reporter effects, (B) UV end point images, (C) natural light detection, (D) Cas12a concentration effects, and (E) Cas12a/crRNA ratio effects.

    Article Snippet: EnGen Lba Cas12a (Cpf1) and NEB buffer 2.1 were purchased from New England Biolabs (NEB, USA).

    Techniques: CRISPR, Concentration Assay

    LOD for nested PCR/CRISPR-Cas12a assay. (A) RdRp region linearity curve, (B) N gene linearity curve, (C) RdRp region LOD, and (D) N gene LOD.

    Journal: ACS Omega

    Article Title: One-Tube Nested PCR Coupled with CRISPR-Cas12a for Ultrasensitive Nucleic Acid Testing

    doi: 10.1021/acsomega.4c03911

    Figure Lengend Snippet: LOD for nested PCR/CRISPR-Cas12a assay. (A) RdRp region linearity curve, (B) N gene linearity curve, (C) RdRp region LOD, and (D) N gene LOD.

    Article Snippet: EnGen Lba Cas12a (Cpf1) and NEB buffer 2.1 were purchased from New England Biolabs (NEB, USA).

    Techniques: Nested PCR, CRISPR

    Real-time fluorescence results for nested PCR/CRISPR-Cas12a assay on clinical specimens.

    Journal: ACS Omega

    Article Title: One-Tube Nested PCR Coupled with CRISPR-Cas12a for Ultrasensitive Nucleic Acid Testing

    doi: 10.1021/acsomega.4c03911

    Figure Lengend Snippet: Real-time fluorescence results for nested PCR/CRISPR-Cas12a assay on clinical specimens.

    Article Snippet: EnGen Lba Cas12a (Cpf1) and NEB buffer 2.1 were purchased from New England Biolabs (NEB, USA).

    Techniques: Fluorescence, Nested PCR, CRISPR

    Concordance analysis between the one-tube nested PCR/CRISPR-Cas12a assay and the RT-qPCR assay.

    Journal: ACS Omega

    Article Title: One-Tube Nested PCR Coupled with CRISPR-Cas12a for Ultrasensitive Nucleic Acid Testing

    doi: 10.1021/acsomega.4c03911

    Figure Lengend Snippet: Concordance analysis between the one-tube nested PCR/CRISPR-Cas12a assay and the RT-qPCR assay.

    Article Snippet: EnGen Lba Cas12a (Cpf1) and NEB buffer 2.1 were purchased from New England Biolabs (NEB, USA).

    Techniques: Nested PCR, CRISPR, Quantitative RT-PCR

    One-tube nested PCR/CRISPR-Cas12a assay schematic. (A) MBs-based viral RNA extraction and (B) assay with visual (fluorescence or naked eye) readout.

    Journal: ACS Omega

    Article Title: One-Tube Nested PCR Coupled with CRISPR-Cas12a for Ultrasensitive Nucleic Acid Testing

    doi: 10.1021/acsomega.4c03911

    Figure Lengend Snippet: One-tube nested PCR/CRISPR-Cas12a assay schematic. (A) MBs-based viral RNA extraction and (B) assay with visual (fluorescence or naked eye) readout.

    Article Snippet: The Cas12a system (20 μL) included 20 nM EnGen Lba Cas12a, 5 μM ssDNA reporter, 100 nM crRNA, 10 × NEB buffer 2.1 (2 μL), and nuclease-free water (12 μL).

    Techniques: Nested PCR, CRISPR, RNA Extraction, Fluorescence

    CRISPR Cas12a detection optimization. (A) ssDNA reporter effects, (B) UV end point images, (C) natural light detection, (D) Cas12a concentration effects, and (E) Cas12a/crRNA ratio effects.

    Journal: ACS Omega

    Article Title: One-Tube Nested PCR Coupled with CRISPR-Cas12a for Ultrasensitive Nucleic Acid Testing

    doi: 10.1021/acsomega.4c03911

    Figure Lengend Snippet: CRISPR Cas12a detection optimization. (A) ssDNA reporter effects, (B) UV end point images, (C) natural light detection, (D) Cas12a concentration effects, and (E) Cas12a/crRNA ratio effects.

    Article Snippet: The Cas12a system (20 μL) included 20 nM EnGen Lba Cas12a, 5 μM ssDNA reporter, 100 nM crRNA, 10 × NEB buffer 2.1 (2 μL), and nuclease-free water (12 μL).

    Techniques: CRISPR, Concentration Assay

    LOD for nested PCR/CRISPR-Cas12a assay. (A) RdRp region linearity curve, (B) N gene linearity curve, (C) RdRp region LOD, and (D) N gene LOD.

    Journal: ACS Omega

    Article Title: One-Tube Nested PCR Coupled with CRISPR-Cas12a for Ultrasensitive Nucleic Acid Testing

    doi: 10.1021/acsomega.4c03911

    Figure Lengend Snippet: LOD for nested PCR/CRISPR-Cas12a assay. (A) RdRp region linearity curve, (B) N gene linearity curve, (C) RdRp region LOD, and (D) N gene LOD.

    Article Snippet: The Cas12a system (20 μL) included 20 nM EnGen Lba Cas12a, 5 μM ssDNA reporter, 100 nM crRNA, 10 × NEB buffer 2.1 (2 μL), and nuclease-free water (12 μL).

    Techniques: Nested PCR, CRISPR

    Real-time fluorescence results for nested PCR/CRISPR-Cas12a assay on clinical specimens.

    Journal: ACS Omega

    Article Title: One-Tube Nested PCR Coupled with CRISPR-Cas12a for Ultrasensitive Nucleic Acid Testing

    doi: 10.1021/acsomega.4c03911

    Figure Lengend Snippet: Real-time fluorescence results for nested PCR/CRISPR-Cas12a assay on clinical specimens.

    Article Snippet: The Cas12a system (20 μL) included 20 nM EnGen Lba Cas12a, 5 μM ssDNA reporter, 100 nM crRNA, 10 × NEB buffer 2.1 (2 μL), and nuclease-free water (12 μL).

    Techniques: Fluorescence, Nested PCR, CRISPR

    Concordance analysis between the one-tube nested PCR/CRISPR-Cas12a assay and the RT-qPCR assay.

    Journal: ACS Omega

    Article Title: One-Tube Nested PCR Coupled with CRISPR-Cas12a for Ultrasensitive Nucleic Acid Testing

    doi: 10.1021/acsomega.4c03911

    Figure Lengend Snippet: Concordance analysis between the one-tube nested PCR/CRISPR-Cas12a assay and the RT-qPCR assay.

    Article Snippet: The Cas12a system (20 μL) included 20 nM EnGen Lba Cas12a, 5 μM ssDNA reporter, 100 nM crRNA, 10 × NEB buffer 2.1 (2 μL), and nuclease-free water (12 μL).

    Techniques: Nested PCR, CRISPR, Quantitative RT-PCR