dna adapter ligation  (New England Biolabs)


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    Name:
    LongAmp Taq 2X Master Mix
    Description:
    LongAmp Taq 2X Master Mix 500 rxns
    Catalog Number:
    m0287l
    Price:
    537
    Size:
    500 rxns
    Category:
    Long distance PCR Kits
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    Structured Review

    New England Biolabs dna adapter ligation
    LongAmp Taq 2X Master Mix
    LongAmp Taq 2X Master Mix 500 rxns
    https://www.bioz.com/result/dna adapter ligation/product/New England Biolabs
    Average 93 stars, based on 395 article reviews
    Price from $9.99 to $1999.99
    dna adapter ligation - by Bioz Stars, 2020-05
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: Rapid virulence prediction and identification of Newcastle disease virus genotypes using third-generation sequencing
    Article Snippet: .. Briefly, each of the amplicons were diluted to 0.5 nM for barcoding and amplified using LongAmp Taq 2X Master Mix (New England Biolabs, USA) with the following conditions; 95 °C for 3 min; 15 cycles of 95 °C for 15 s; 62 °C for 15 s, 65 °C for 50 s, followed by 65 °C for 50 s. The barcoded amplicons were bead purified, pooled into a single tube, end prepped, dA tailed, bead purified, and ligated to the sequencing adapters per manufacturer’s instructions. .. Final DNA libraries were bead purified and stored frozen until used for sequencing.

    Polymerase Chain Reaction:

    Article Title: Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencer
    Article Snippet: .. PCR amplification of 16S rRNA genes was conducted using the 16S Barcoding Kit (SQK‐RAB204; Oxford Nanopore Technologies, Oxford, UK) containing the 27F/1492R primer set , and LongAmp™ Taq 2× Master Mix (New England Biolabs, Ipswich, MA, USA). .. Amplification was performed using an Applied Biosystems Veriti™ Thermal Cycler (Thermo Fischer Scientific, Waltham, MA, USA) with the following PCR conditions: initial denaturation at 95 °C for 3 min, 25 cycles of 95 °C for 20 s, 55 °C for 30 s, and 65 °C for 2 min, followed by a final extension at 65 °C for 5 min. To determine the effects of human DNA contamination on 16S rRNA gene amplification, genomic DNA purified from the human monocytic cell line THP‐1 was mixed with E. coli DNA and subjected to PCR.

    Article Title: Preparation of Fragment Libraries for Next-Generation Sequencing on the Applied Biosystems SOLiD Platform
    Article Snippet: .. SOLiD Library P1 adaptor plus strand (5′-CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGAT-3′) SOLiD Library P1 adaptor minus strand (5′-ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGTT-3′) SOLiD Library P2 adaptor plus strand (5′-AGAGAATGAGGAACCCGGGGCAGTT-3′) SOLiD Library P2 adaptor minus strand (5′-CTGCCCCGGGTTCCTCATTCTCT-3′) SOLiD Library PCR primer 1 (5′-CCACTACGCCTCCGCTTTCCTCTCTATG-3′) SOLiD Library PCR primer 2 (5′-CTGCCCCGGGTTCCTCATTCT-3′) NEBNext End Repair Reaction Buffer Mix (10×) (NEB, E6062A or E6062AA, or as part of E6060S/L, or see recipe below) NEBNext End Repair Enzyme Mix (NEB, E6061A or E6061AA, or as part of E6060S/L) SOLiD Library Column Purification Kit (Applied Biosystems, 4443744) NEBNext Quick Ligation Reaction Buffer (NEB, E6064A or E6064AA, or as part of E6060S/L, or see recipe below) T4 DNA Ligase (NEB, E6063A or E6063AA or as part of E6060S/L) LongAmp Taq 2× Master Mix (NEB, E6065A or E6065AA or as part of E6060S/L) E-Gels compatible with E-Gel iBase system Tris base Hydrochloric acid (HCl) EDTA Nuclease-free water Isopropyl alcohol Ethylene glycol 50-bp DNA ladder (e.g., Invitrogen 10416-014) .. Step 1 1× Low TE Buffer Step 2 10× End Repair Reaction Buffer Mix Step 3 and also used in Preparation of Adaptor Oligos 5×NEBNext Quick Ligation Reaction Buffer

    Article Title: Quasimetagenomics-Based and Real-Time-Sequencing-Aided Detection and Subtyping of Salmonella enterica from Food Samples
    Article Snippet: .. The adapted DNA was mixed with 2× LongAmp Taq master mix (New England BioLabs) and a PCR was run for 18 cycles. ..

    Purification:

    Article Title: Rapid virulence prediction and identification of Newcastle disease virus genotypes using third-generation sequencing
    Article Snippet: .. Briefly, each of the amplicons were diluted to 0.5 nM for barcoding and amplified using LongAmp Taq 2X Master Mix (New England Biolabs, USA) with the following conditions; 95 °C for 3 min; 15 cycles of 95 °C for 15 s; 62 °C for 15 s, 65 °C for 50 s, followed by 65 °C for 50 s. The barcoded amplicons were bead purified, pooled into a single tube, end prepped, dA tailed, bead purified, and ligated to the sequencing adapters per manufacturer’s instructions. .. Final DNA libraries were bead purified and stored frozen until used for sequencing.

    Article Title: Preparation of Fragment Libraries for Next-Generation Sequencing on the Applied Biosystems SOLiD Platform
    Article Snippet: .. SOLiD Library P1 adaptor plus strand (5′-CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGAT-3′) SOLiD Library P1 adaptor minus strand (5′-ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGTT-3′) SOLiD Library P2 adaptor plus strand (5′-AGAGAATGAGGAACCCGGGGCAGTT-3′) SOLiD Library P2 adaptor minus strand (5′-CTGCCCCGGGTTCCTCATTCTCT-3′) SOLiD Library PCR primer 1 (5′-CCACTACGCCTCCGCTTTCCTCTCTATG-3′) SOLiD Library PCR primer 2 (5′-CTGCCCCGGGTTCCTCATTCT-3′) NEBNext End Repair Reaction Buffer Mix (10×) (NEB, E6062A or E6062AA, or as part of E6060S/L, or see recipe below) NEBNext End Repair Enzyme Mix (NEB, E6061A or E6061AA, or as part of E6060S/L) SOLiD Library Column Purification Kit (Applied Biosystems, 4443744) NEBNext Quick Ligation Reaction Buffer (NEB, E6064A or E6064AA, or as part of E6060S/L, or see recipe below) T4 DNA Ligase (NEB, E6063A or E6063AA or as part of E6060S/L) LongAmp Taq 2× Master Mix (NEB, E6065A or E6065AA or as part of E6060S/L) E-Gels compatible with E-Gel iBase system Tris base Hydrochloric acid (HCl) EDTA Nuclease-free water Isopropyl alcohol Ethylene glycol 50-bp DNA ladder (e.g., Invitrogen 10416-014) .. Step 1 1× Low TE Buffer Step 2 10× End Repair Reaction Buffer Mix Step 3 and also used in Preparation of Adaptor Oligos 5×NEBNext Quick Ligation Reaction Buffer

    Amplification:

    Article Title: Rapid virulence prediction and identification of Newcastle disease virus genotypes using third-generation sequencing
    Article Snippet: .. Briefly, each of the amplicons were diluted to 0.5 nM for barcoding and amplified using LongAmp Taq 2X Master Mix (New England Biolabs, USA) with the following conditions; 95 °C for 3 min; 15 cycles of 95 °C for 15 s; 62 °C for 15 s, 65 °C for 50 s, followed by 65 °C for 50 s. The barcoded amplicons were bead purified, pooled into a single tube, end prepped, dA tailed, bead purified, and ligated to the sequencing adapters per manufacturer’s instructions. .. Final DNA libraries were bead purified and stored frozen until used for sequencing.

    Article Title: Denaturing CLIP (dCLIP) pipeline identifies discrete RNA footprints on chromatin-associated proteins and reveals that CBX7 targets 3’UTRs to regulate mRNA expression
    Article Snippet: .. Amplification was performed with LongAmp™ Taq 2× Master Mix (NEB). .. Amplified PCR products were subjected to PAGE electrophoresis on 6% TBE-acrylamide gel.

    Article Title: Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencer
    Article Snippet: .. PCR amplification of 16S rRNA genes was conducted using the 16S Barcoding Kit (SQK‐RAB204; Oxford Nanopore Technologies, Oxford, UK) containing the 27F/1492R primer set , and LongAmp™ Taq 2× Master Mix (New England Biolabs, Ipswich, MA, USA). .. Amplification was performed using an Applied Biosystems Veriti™ Thermal Cycler (Thermo Fischer Scientific, Waltham, MA, USA) with the following PCR conditions: initial denaturation at 95 °C for 3 min, 25 cycles of 95 °C for 20 s, 55 °C for 30 s, and 65 °C for 2 min, followed by a final extension at 65 °C for 5 min. To determine the effects of human DNA contamination on 16S rRNA gene amplification, genomic DNA purified from the human monocytic cell line THP‐1 was mixed with E. coli DNA and subjected to PCR.

    Article Title: Pseudo-De Novo Assembly and Analysis of Unmapped Genome Sequence Reads in Wild Zebrafish Reveal Novel Gene Content
    Article Snippet: .. Sequences were amplified with LongAmp Taq Master Mix (NEB) according to the manufacturer's protocol with a 59°C annealing temperature and 5-min elongation step. ..

    Ligation:

    Article Title: Preparation of Fragment Libraries for Next-Generation Sequencing on the Applied Biosystems SOLiD Platform
    Article Snippet: .. SOLiD Library P1 adaptor plus strand (5′-CCACTACGCCTCCGCTTTCCTCTCTATGGGCAGTCGGTGAT-3′) SOLiD Library P1 adaptor minus strand (5′-ATCACCGACTGCCCATAGAGAGGAAAGCGGAGGCGTAGTGGTT-3′) SOLiD Library P2 adaptor plus strand (5′-AGAGAATGAGGAACCCGGGGCAGTT-3′) SOLiD Library P2 adaptor minus strand (5′-CTGCCCCGGGTTCCTCATTCTCT-3′) SOLiD Library PCR primer 1 (5′-CCACTACGCCTCCGCTTTCCTCTCTATG-3′) SOLiD Library PCR primer 2 (5′-CTGCCCCGGGTTCCTCATTCT-3′) NEBNext End Repair Reaction Buffer Mix (10×) (NEB, E6062A or E6062AA, or as part of E6060S/L, or see recipe below) NEBNext End Repair Enzyme Mix (NEB, E6061A or E6061AA, or as part of E6060S/L) SOLiD Library Column Purification Kit (Applied Biosystems, 4443744) NEBNext Quick Ligation Reaction Buffer (NEB, E6064A or E6064AA, or as part of E6060S/L, or see recipe below) T4 DNA Ligase (NEB, E6063A or E6063AA or as part of E6060S/L) LongAmp Taq 2× Master Mix (NEB, E6065A or E6065AA or as part of E6060S/L) E-Gels compatible with E-Gel iBase system Tris base Hydrochloric acid (HCl) EDTA Nuclease-free water Isopropyl alcohol Ethylene glycol 50-bp DNA ladder (e.g., Invitrogen 10416-014) .. Step 1 1× Low TE Buffer Step 2 10× End Repair Reaction Buffer Mix Step 3 and also used in Preparation of Adaptor Oligos 5×NEBNext Quick Ligation Reaction Buffer

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  • 96
    New England Biolabs nebnext endrepair module
    Nebnext Endrepair Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext endrepair module/product/New England Biolabs
    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    nebnext endrepair module - by Bioz Stars, 2020-05
    96/100 stars
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