endogenous peroxidase  (Vector Laboratories)


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    Structured Review

    Vector Laboratories endogenous peroxidase
    Endogenous Peroxidase, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endogenous peroxidase/product/Vector Laboratories
    Average 94 stars, based on 398 article reviews
    Price from $9.99 to $1999.99
    endogenous peroxidase - by Bioz Stars, 2020-11
    94/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Temporal profiles of synaptic plasticity-related signals in adult mouse hippocampus with methotrexate treatment ☆
    Article Snippet: .. For immunohistochemistry, the sagittal sections were deactivated with endogenous peroxidase (5% hydrogen peroxidase in methyl alcohol and 0.2% Triton X 100) and blocked with 10% normal goat serum (ABC Elite Kit; Vector Laboratories, Burlingame, CA, USA) in PBS for 1 hour. .. Sections were then incubated with primary antibodies, including rabbit anti-p-ERK1/2 (1:500; Cell Signaling Technology), rabbit anti-p-CREB (1:500 dilution; Cell Signaling Technology), rabbit anti-p-NMDAR1 (1:100; Cell Signaling Technology), rabbit p-CaMKII (1:200; Cell Signaling Technology) and rabbit anti-p-GluR1 ser831 (1:100; Millipore) in PBS-T overnight at 4°C.

    Article Title: Single-walled carbon nanotubes increase pandemic influenza A H1N1 virus infectivity of lung epithelial cells
    Article Snippet: .. Lectin immunohistochemistry SAEC plated on coverslips were fixed in acetone, washed with PBS, and endogenous peroxidase was quenched by incubation BLOXALL™ Blocking Solution (Vector Laboratories, CA) for 10 min at room temperature. .. Cells were then washed three times with PBS and blocked in 3% (w/v) bovine serum albumin (BSA) for 1 h at 4°C prior to adding 20 μg/ml biotinylated Maackia amurensis lectin II (MAA, Vector Laboratories) or 10 μg/ml biotinylated Sambucus nigra (elderberry) bark lectin (SNA, Vector Laboratories) overnight at 4°C.

    Avidin-Biotin Assay:

    Article Title: Cationized liposomal keto-mycolic acids isolated from Mycobacterium bovis bacillus Calmette-Guérin induce antitumor immunity in a syngeneic murine bladder cancer model
    Article Snippet: .. Endogenous peroxidase was blocked with 0.3% H2 O2 in methanol for 30 min, followed by incubation with G-Block (Genostaff) and an Avidin/Biotin Blocking Kit (Vector Laboratories, Burlingame, CA). .. The sections were incubated with CD4 rat mAb or CD8 rat mAb at 4°C overnight.

    Article Title: The Antidepressant Mirtazapine Inhibits Hepatic Innate Immune Networks to Attenuate Immune-Mediated Liver Injury in Mice
    Article Snippet: .. Endogenous peroxidase and endogenous biotin binding were blocked using 3% H2 O2 and an avidin/biotin blocking kit (Vector Laboratories, Burlingame, CA), respectively. .. Slides were incubated with rat primary anti-mouse Ly6G monoclonal antibody (1 μg/ml overnight at 4°C) followed by incubation with biotinylated goat anti-rat secondary antibody (1:150 dilution.

    Blocking Assay:

    Article Title: Assessment of Control Tissue for Gene and Protein Expression Studies: A Comparison of Three Alternative Lung Sources
    Article Snippet: .. Prior to the application of primary antibodies, the sections were subjected to antigen retrieval by heating at 95°C for 20 mins in citrate buffer followed by blocking endogenous peroxidase by incubating them in 3% H2 O2 for 5 min. Nonspecific binding was blocked by incubating them in 10% normal horse serum for 1 hr (Vector Lab, Calif, USA). .. Primary antibodies against VEGF, PGF (1 : 200), FLT1, KDR, TIMP-2 (1 : 200), MMP-2, TIMP-1 (1 : 100), and MMP-9 (1 : 40) were purchased from Santa Cruz (Calif, USA).

    Article Title: Single-walled carbon nanotubes increase pandemic influenza A H1N1 virus infectivity of lung epithelial cells
    Article Snippet: .. Lectin immunohistochemistry SAEC plated on coverslips were fixed in acetone, washed with PBS, and endogenous peroxidase was quenched by incubation BLOXALL™ Blocking Solution (Vector Laboratories, CA) for 10 min at room temperature. .. Cells were then washed three times with PBS and blocked in 3% (w/v) bovine serum albumin (BSA) for 1 h at 4°C prior to adding 20 μg/ml biotinylated Maackia amurensis lectin II (MAA, Vector Laboratories) or 10 μg/ml biotinylated Sambucus nigra (elderberry) bark lectin (SNA, Vector Laboratories) overnight at 4°C.

    Article Title: Clinical significance of PD-1/PD-Ls gene amplification and overexpression in patients with hepatocellular carcinoma
    Article Snippet: .. After blocking endogenous peroxidase and nonspecific binding sites (ZAE-ICT-6295-L100, ENZO), primary Abs and secondary HRP-conjugated polymers (MPX-2402, Vectorlabs) were applied. .. Each HRP-conjugated polymer covalently binds a distinct fluorophore using tyramide signal amplification (Opal 7-color Fluorophore TSA plus Fluorescence Kit (NEL 797001KT; PerkinElmer)).

    Article Title: The SIRT1 Deacetylase Suppresses Intestinal Tumorigenesis and Colon Cancer Growth
    Article Snippet: .. Tissue sections were incubated with 3% H2 O2 (15 min) to block endogenous peroxidase, then incubated with 10% normal goat serum (Vector Laboratories, Burlingame, CA) in PBS (10 min), followed by 10 min incubation in serum free protein block (DAKO, Carpinteria, CA). .. Primary antibody against β-catenin (clone 14, BD Transduction Laboratories, Franklin Lakes, NJ) (dilution 1∶400) or SIRT1 (#1104; Epitomics) (dilution 1∶100) was applied for 1 hour at room temperature.

    Article Title: Cationized liposomal keto-mycolic acids isolated from Mycobacterium bovis bacillus Calmette-Guérin induce antitumor immunity in a syngeneic murine bladder cancer model
    Article Snippet: .. Endogenous peroxidase was blocked with 0.3% H2 O2 in methanol for 30 min, followed by incubation with G-Block (Genostaff) and an Avidin/Biotin Blocking Kit (Vector Laboratories, Burlingame, CA). .. The sections were incubated with CD4 rat mAb or CD8 rat mAb at 4°C overnight.

    Article Title: The Antidepressant Mirtazapine Inhibits Hepatic Innate Immune Networks to Attenuate Immune-Mediated Liver Injury in Mice
    Article Snippet: .. Endogenous peroxidase and endogenous biotin binding were blocked using 3% H2 O2 and an avidin/biotin blocking kit (Vector Laboratories, Burlingame, CA), respectively. .. Slides were incubated with rat primary anti-mouse Ly6G monoclonal antibody (1 μg/ml overnight at 4°C) followed by incubation with biotinylated goat anti-rat secondary antibody (1:150 dilution.

    Immunodetection:

    Article Title: Expression Characteristics of Genes Hypermethylated and Downregulated in Rat Liver Specific to Nongenotoxic Hepatocarcinogens
    Article Snippet: .. To inhibit endogenous peroxidase, deparaffinized sections were incubated in 0.3% (v/v) hydrogen peroxide solution in absolute methanol for 30 min. Immunodetection was performed using a Vectastain Elite ABC Kit (Vector Laboratories Inc., Burlingame, California) with the primary antibodies and 3, 3′,diaminobenzidine/H2 O2 as the chromogen. ..

    Incubation:

    Article Title: Single-walled carbon nanotubes increase pandemic influenza A H1N1 virus infectivity of lung epithelial cells
    Article Snippet: .. Lectin immunohistochemistry SAEC plated on coverslips were fixed in acetone, washed with PBS, and endogenous peroxidase was quenched by incubation BLOXALL™ Blocking Solution (Vector Laboratories, CA) for 10 min at room temperature. .. Cells were then washed three times with PBS and blocked in 3% (w/v) bovine serum albumin (BSA) for 1 h at 4°C prior to adding 20 μg/ml biotinylated Maackia amurensis lectin II (MAA, Vector Laboratories) or 10 μg/ml biotinylated Sambucus nigra (elderberry) bark lectin (SNA, Vector Laboratories) overnight at 4°C.

    Article Title: The SIRT1 Deacetylase Suppresses Intestinal Tumorigenesis and Colon Cancer Growth
    Article Snippet: .. Tissue sections were incubated with 3% H2 O2 (15 min) to block endogenous peroxidase, then incubated with 10% normal goat serum (Vector Laboratories, Burlingame, CA) in PBS (10 min), followed by 10 min incubation in serum free protein block (DAKO, Carpinteria, CA). .. Primary antibody against β-catenin (clone 14, BD Transduction Laboratories, Franklin Lakes, NJ) (dilution 1∶400) or SIRT1 (#1104; Epitomics) (dilution 1∶100) was applied for 1 hour at room temperature.

    Article Title: Expression Characteristics of Genes Hypermethylated and Downregulated in Rat Liver Specific to Nongenotoxic Hepatocarcinogens
    Article Snippet: .. To inhibit endogenous peroxidase, deparaffinized sections were incubated in 0.3% (v/v) hydrogen peroxide solution in absolute methanol for 30 min. Immunodetection was performed using a Vectastain Elite ABC Kit (Vector Laboratories Inc., Burlingame, California) with the primary antibodies and 3, 3′,diaminobenzidine/H2 O2 as the chromogen. ..

    Article Title: Cationized liposomal keto-mycolic acids isolated from Mycobacterium bovis bacillus Calmette-Guérin induce antitumor immunity in a syngeneic murine bladder cancer model
    Article Snippet: .. Endogenous peroxidase was blocked with 0.3% H2 O2 in methanol for 30 min, followed by incubation with G-Block (Genostaff) and an Avidin/Biotin Blocking Kit (Vector Laboratories, Burlingame, CA). .. The sections were incubated with CD4 rat mAb or CD8 rat mAb at 4°C overnight.

    Binding Assay:

    Article Title: Assessment of Control Tissue for Gene and Protein Expression Studies: A Comparison of Three Alternative Lung Sources
    Article Snippet: .. Prior to the application of primary antibodies, the sections were subjected to antigen retrieval by heating at 95°C for 20 mins in citrate buffer followed by blocking endogenous peroxidase by incubating them in 3% H2 O2 for 5 min. Nonspecific binding was blocked by incubating them in 10% normal horse serum for 1 hr (Vector Lab, Calif, USA). .. Primary antibodies against VEGF, PGF (1 : 200), FLT1, KDR, TIMP-2 (1 : 200), MMP-2, TIMP-1 (1 : 100), and MMP-9 (1 : 40) were purchased from Santa Cruz (Calif, USA).

    Article Title: Clinical significance of PD-1/PD-Ls gene amplification and overexpression in patients with hepatocellular carcinoma
    Article Snippet: .. After blocking endogenous peroxidase and nonspecific binding sites (ZAE-ICT-6295-L100, ENZO), primary Abs and secondary HRP-conjugated polymers (MPX-2402, Vectorlabs) were applied. .. Each HRP-conjugated polymer covalently binds a distinct fluorophore using tyramide signal amplification (Opal 7-color Fluorophore TSA plus Fluorescence Kit (NEL 797001KT; PerkinElmer)).

    Article Title: The Antidepressant Mirtazapine Inhibits Hepatic Innate Immune Networks to Attenuate Immune-Mediated Liver Injury in Mice
    Article Snippet: .. Endogenous peroxidase and endogenous biotin binding were blocked using 3% H2 O2 and an avidin/biotin blocking kit (Vector Laboratories, Burlingame, CA), respectively. .. Slides were incubated with rat primary anti-mouse Ly6G monoclonal antibody (1 μg/ml overnight at 4°C) followed by incubation with biotinylated goat anti-rat secondary antibody (1:150 dilution.

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Vector Laboratories endogenous peroxidase
    Endogenous Peroxidase, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 398 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endogenous peroxidase/product/Vector Laboratories
    Average 94 stars, based on 398 article reviews
    Price from $9.99 to $1999.99
    endogenous peroxidase - by Bioz Stars, 2020-11
    94/100 stars
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