Structured Review

Merck & Co endogenous peroxidase
Endogenous Peroxidase, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/endogenous peroxidase/product/Merck & Co
Average 92 stars, based on 31 article reviews
Price from $9.99 to $1999.99
endogenous peroxidase - by Bioz Stars, 2020-11
92/100 stars

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Staining:

Article Title: Force‐transmitting structures in the digital pads of the tree frog Hyla cinerea: a functional interpretation
Article Snippet: .. Before immunohistochemical staining (see Appendix ), the sections were deparaffinised towards demineralised water, endogenous peroxidase was removed with a solution of 0.3% H2 O2 (Merck) in buffer, and aspecific antibody‐binding was blocked by the application of 10% goat serum (Vector Laboratories, USA) in combination with 1% acetylated bovine serum albumin (BSA‐c; Aurion, Netherlands). .. The primary α‐actin‐antibody was applied overnight at 4 °C (dilution 1:400), followed by a secondary goat‐anti‐mouse/horseradish‐peroxidase‐antibody (Agilent, Canada) for 45 min (dilution 1:100).

Incubation:

Article Title: T‐cell infiltration and clonality correlate with programmed cell death protein 1 and programmed death‐ligand 1 expression in patients with soft tissue sarcomas
Article Snippet: .. Endogenous peroxidase in tissues was blocked by incubation of slides in 3% hydrogen peroxide solution before incubation with the primary antibody (anti‐PD‐L1 clone 22C3 [Merck Research Laboratories, Palo Alto, Calif] or anti‐PD‐1 clone NAT105 [Cell Marque, Rocklin, Calif]) for 60 minutes. .. Antigen‐antibody binding was visualized with the FLEX + polymer system (Dako) and application of 3,3' diaminobenzidine chromogen (Dako).

Article Title: Adhering maternal platelets can contribute to the cytokine and chemokine cocktail released by human first trimester villous placenta
Article Snippet: .. In brief, endogenous peroxidase was blocked using the hydrogen peroxidase block for 10 min. After three washing steps with TBS including 0.05% Tween 20 (TBS/T; Merck) background blocking was performed using Ultra Vision Protein Block for 5 min. Primary antibodies (see ) were diluted in Antibody Diluent (Dako) and incubated on slides for 45 min at RT. .. Thereafter slides were washed three times and detection achieved by incubation with anti-rabbit UltraVision HRP-labelled polymer (15 min) and 3-amino-9-ethylcarbacole (AEC, Thermo Scientific) for 10 min.

Article Title: A Single Dose of Neoadjuvant PD-1 Blockade Predicts Clinical Outcomes in Resectable Melanoma
Article Snippet: .. Endogenous peroxidase in tissues was blocked by incubation of slides in 3% hydrogen peroxide solution prior to incubation with primary antibody (anti-PD-L1, clone 22C3, Merck Research Laboratories, Palo Alto CA or anti-PD-1 clone NAT105, Cell Marque, Rocklin CA) for 60 minutes. .. Antigen-antibody binding was visualized via application of the FLEX+ polymer system (Dako, Carpineteria CA) via application of mouse Envision system, and application of 3, 3’ diaminobenzidine (DAB) chromogen (Dako, Carpineteria CA).

Article Title: Spontaneous Spongiform Brainstem Degeneration in a Young Mouse Lemur (Microcebus murinus) with Conspicuous Behavioral, Motor, Growth, and Ocular Pathologies
Article Snippet: .. Briefly, 3-µm sections were pretreated for 30 min in 98% to 100% formic acid, followed by blocking of endogenous peroxidase with 3% (v/v) H2 O2 (Merck, Kenilworth, NJ) in methanol for 30 min at room temperature and incubation in 4 µg/mL proteinase K for another 15 min at 37 °C. .. As a primary antibody, the prion-protein–specific monoclonal antibody L42 (0.1 µg/mL) was incubated for 30 min at room temperature.

Article Title: Programmed Death-1 Blockade With Pembrolizumab in Patients With Classical Hodgkin Lymphoma After Brentuximab Vedotin Failure
Article Snippet: .. Endogenous peroxidase in tissues was blocked by incubation of slides in 3% hydrogen peroxide solution prior to incubation with primary antibody (anti–PD-L2 clone 3G2; Merck Research Laboratories) for 60 minutes. .. Antigen-antibody binding was visualized via application of the FLEX+ polymer system (K8012; Dako) and application of 3,3′ diaminobenzidine chromogen (K4368, Dako).

Immunohistochemistry:

Article Title: Force‐transmitting structures in the digital pads of the tree frog Hyla cinerea: a functional interpretation
Article Snippet: .. Before immunohistochemical staining (see Appendix ), the sections were deparaffinised towards demineralised water, endogenous peroxidase was removed with a solution of 0.3% H2 O2 (Merck) in buffer, and aspecific antibody‐binding was blocked by the application of 10% goat serum (Vector Laboratories, USA) in combination with 1% acetylated bovine serum albumin (BSA‐c; Aurion, Netherlands). .. The primary α‐actin‐antibody was applied overnight at 4 °C (dilution 1:400), followed by a secondary goat‐anti‐mouse/horseradish‐peroxidase‐antibody (Agilent, Canada) for 45 min (dilution 1:100).

Blocking Assay:

Article Title: Adhering maternal platelets can contribute to the cytokine and chemokine cocktail released by human first trimester villous placenta
Article Snippet: .. In brief, endogenous peroxidase was blocked using the hydrogen peroxidase block for 10 min. After three washing steps with TBS including 0.05% Tween 20 (TBS/T; Merck) background blocking was performed using Ultra Vision Protein Block for 5 min. Primary antibodies (see ) were diluted in Antibody Diluent (Dako) and incubated on slides for 45 min at RT. .. Thereafter slides were washed three times and detection achieved by incubation with anti-rabbit UltraVision HRP-labelled polymer (15 min) and 3-amino-9-ethylcarbacole (AEC, Thermo Scientific) for 10 min.

Article Title: Spontaneous Spongiform Brainstem Degeneration in a Young Mouse Lemur (Microcebus murinus) with Conspicuous Behavioral, Motor, Growth, and Ocular Pathologies
Article Snippet: .. Briefly, 3-µm sections were pretreated for 30 min in 98% to 100% formic acid, followed by blocking of endogenous peroxidase with 3% (v/v) H2 O2 (Merck, Kenilworth, NJ) in methanol for 30 min at room temperature and incubation in 4 µg/mL proteinase K for another 15 min at 37 °C. .. As a primary antibody, the prion-protein–specific monoclonal antibody L42 (0.1 µg/mL) was incubated for 30 min at room temperature.

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    Merck & Co endogenous peroxidase
    Endogenous Peroxidase, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endogenous peroxidase/product/Merck & Co
    Average 92 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    endogenous peroxidase - by Bioz Stars, 2020-11
    92/100 stars
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