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Ventana Medical endogenous peroxidase activity
Endogenous Peroxidase Activity, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 92/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/endogenous peroxidase activity/product/Ventana Medical
Average 92 stars, based on 46 article reviews
Price from $9.99 to $1999.99
endogenous peroxidase activity - by Bioz Stars, 2020-11
92/100 stars

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Related Articles

Staining:

Article Title: Folylpoly-Glutamate Synthetase Expression is associated with Tumor Response and Outcome from Pemetrexed-based Chemotherapy in Malignant Pleural Mesothelioma
Article Snippet: .. Deparaffinization, rehydration, blocking of endogenous peroxidase activity with hydrogen peroxide and staining visualization was accomplished using an ultra View Universial DAB detection kit (Ventana, Tucson, AZ, USA) on an automated staining system (BENCHMARK XT, Ventana). .. The slides were incubated for 1 hour at 37°C or at room temperature with the respective primary antibodies: rabbit polyclonal anti-FPGS antibody (dilution 1:20, Spring Bioscience, Inc., Pleasanton, CA, USA) or mouse monoclonal anti-TS antibody (dilution 1:10, Invitrogen, Inc., Carlsbad, CA, USA).

Article Title: EFHD2 contributes to non-small cell lung cancer cisplatin resistance by the activation of NOX4-ROS-ABCC1 axis
Article Snippet: .. After inactivating endogenous peroxidase activity, rabbit anti-human EFHD2 and ABCC1 polyclonal antibodies were used for IHC staining, which was performed by an automatic BenchMark XT staining machine using iVIEW 3,3-diaminobenzidine (DAB) detection kit (Ventana Medical Systems). .. Tumor sections were finally incubated with iVIEW copper to enhance signal intensity.

Article Title: Pancreatic radiation effect in apoptosis-related rectal radiation toxicity
Article Snippet: .. Following section deparaffinization and an an antigen-retrieval for 60 min, endogenous peroxidase activity was blocked by exposing the sections to an ultraviolet inhibitor for 4 min. After the reaction buffer washing, an Ultra View Universal DAB Detection Kit (Ventana Medical Systems, Tucson, AZ) was used for IHC staining. .. The slides were incubated with monoclonal antibodies against CCP3 (1:100, #9664, Cell Signaling Technology, Inc., Danvers, MA) for 1 h at 37°C in an autostainer (Benchmark XT, Ventana Medical Systems) and rinsed with the reaction buffer.

Article Title: Sex hormone receptor expression and survival in esophageal adenocarcinoma: a prospective cohort study
Article Snippet: .. A 3-μm thick section was deparafinized and endogenous peroxidase activity quenched with 0.3% hydrogen peroxide prior to slide labelling and staining using the Ventana Discovery XT® automated immunostainer (Ventana Medical Systems Inc, Tuscon, AZ) or the Bond Automated IHC/ISH Stainer (Leica Biosystems, Milton Keynes, UK). .. Slides were stained with rabbit (ERα) or mouse (AR, ERβ) monoclonal antibodies specific to the relevant sex hormone receptor.

Immunohistochemistry:

Article Title: EFHD2 contributes to non-small cell lung cancer cisplatin resistance by the activation of NOX4-ROS-ABCC1 axis
Article Snippet: .. After inactivating endogenous peroxidase activity, rabbit anti-human EFHD2 and ABCC1 polyclonal antibodies were used for IHC staining, which was performed by an automatic BenchMark XT staining machine using iVIEW 3,3-diaminobenzidine (DAB) detection kit (Ventana Medical Systems). .. Tumor sections were finally incubated with iVIEW copper to enhance signal intensity.

Article Title: Pancreatic radiation effect in apoptosis-related rectal radiation toxicity
Article Snippet: .. Following section deparaffinization and an an antigen-retrieval for 60 min, endogenous peroxidase activity was blocked by exposing the sections to an ultraviolet inhibitor for 4 min. After the reaction buffer washing, an Ultra View Universal DAB Detection Kit (Ventana Medical Systems, Tucson, AZ) was used for IHC staining. .. The slides were incubated with monoclonal antibodies against CCP3 (1:100, #9664, Cell Signaling Technology, Inc., Danvers, MA) for 1 h at 37°C in an autostainer (Benchmark XT, Ventana Medical Systems) and rinsed with the reaction buffer.

Article Title: Sex hormone receptor expression and survival in esophageal adenocarcinoma: a prospective cohort study
Article Snippet: .. A 3-μm thick section was deparafinized and endogenous peroxidase activity quenched with 0.3% hydrogen peroxide prior to slide labelling and staining using the Ventana Discovery XT® automated immunostainer (Ventana Medical Systems Inc, Tuscon, AZ) or the Bond Automated IHC/ISH Stainer (Leica Biosystems, Milton Keynes, UK). .. Slides were stained with rabbit (ERα) or mouse (AR, ERβ) monoclonal antibodies specific to the relevant sex hormone receptor.

Activity Assay:

Article Title: Folylpoly-Glutamate Synthetase Expression is associated with Tumor Response and Outcome from Pemetrexed-based Chemotherapy in Malignant Pleural Mesothelioma
Article Snippet: .. Deparaffinization, rehydration, blocking of endogenous peroxidase activity with hydrogen peroxide and staining visualization was accomplished using an ultra View Universial DAB detection kit (Ventana, Tucson, AZ, USA) on an automated staining system (BENCHMARK XT, Ventana). .. The slides were incubated for 1 hour at 37°C or at room temperature with the respective primary antibodies: rabbit polyclonal anti-FPGS antibody (dilution 1:20, Spring Bioscience, Inc., Pleasanton, CA, USA) or mouse monoclonal anti-TS antibody (dilution 1:10, Invitrogen, Inc., Carlsbad, CA, USA).

Article Title: EFHD2 contributes to non-small cell lung cancer cisplatin resistance by the activation of NOX4-ROS-ABCC1 axis
Article Snippet: .. After inactivating endogenous peroxidase activity, rabbit anti-human EFHD2 and ABCC1 polyclonal antibodies were used for IHC staining, which was performed by an automatic BenchMark XT staining machine using iVIEW 3,3-diaminobenzidine (DAB) detection kit (Ventana Medical Systems). .. Tumor sections were finally incubated with iVIEW copper to enhance signal intensity.

Article Title: Comparison of thyroid transcription factor-1 expression by two monoclonal antibodies in pulmonary and non-pulmonary primary tumors
Article Snippet: .. Reduction of endogenous peroxidase activity, background blocking and chromogen system was performed by using the Discovery DAB Map kit (RUO) from Ventana following the manufacturer protocol. ..

Article Title: Pancreatic radiation effect in apoptosis-related rectal radiation toxicity
Article Snippet: .. Following section deparaffinization and an an antigen-retrieval for 60 min, endogenous peroxidase activity was blocked by exposing the sections to an ultraviolet inhibitor for 4 min. After the reaction buffer washing, an Ultra View Universal DAB Detection Kit (Ventana Medical Systems, Tucson, AZ) was used for IHC staining. .. The slides were incubated with monoclonal antibodies against CCP3 (1:100, #9664, Cell Signaling Technology, Inc., Danvers, MA) for 1 h at 37°C in an autostainer (Benchmark XT, Ventana Medical Systems) and rinsed with the reaction buffer.

Article Title: Nuclear localization of the CK2α-subunit correlates with poor prognosis in clear cell renal cell carcinoma
Article Snippet: .. Sections were blocked for endogenous peroxidase activity using H2 O2 and treated enzymatically with protease 1 for 4 min (760-2018, Ventana Medical System, Tucson, AZ, USA) for epitope retrieval. .. The TMAs were then incubated for 32 min at 36°C with the primary antibody against VHL (SC-5575, Santa Cruz Biotechnology, TX, USA) at a dilution of 1:400.

Article Title: Dynamic Contrast-Enhanced and Diffusion MRI Show Rapid and Dramatic Changes in Tumor Microenvironment in Response to Inhibition of HIF-1? Using PX-478 1
Article Snippet: .. Endogenous peroxidase activity was quenched using a hydrogen peroxide-based inhibitor (DAB Basic Detection Kit; Ventana Medical Systems, Tucson, AZ) and endogenous biotin blocked using an AB Blocking Kit (Ventana Medical Systems). .. The slides were incubated for 32 minutes at 42°C with the mouse monoclonal antihuman HIF-1α (Transduction Laboratories, Lexington, KY) at 10 g/ml.

Article Title: Orai3 is a predictive marker of metastasis and survival in resectable lung adenocarcinoma
Article Snippet: .. The endogenous peroxidase activity was blocked (Ventana) before the antigen retrieval. .. The Cell Conditioning Solution CC1 (Ventana, BenchMark) was then used for antigen retrieval.

Article Title: Sex hormone receptor expression and survival in esophageal adenocarcinoma: a prospective cohort study
Article Snippet: .. A 3-μm thick section was deparafinized and endogenous peroxidase activity quenched with 0.3% hydrogen peroxide prior to slide labelling and staining using the Ventana Discovery XT® automated immunostainer (Ventana Medical Systems Inc, Tuscon, AZ) or the Bond Automated IHC/ISH Stainer (Leica Biosystems, Milton Keynes, UK). .. Slides were stained with rabbit (ERα) or mouse (AR, ERβ) monoclonal antibodies specific to the relevant sex hormone receptor.

Blocking Assay:

Article Title: Folylpoly-Glutamate Synthetase Expression is associated with Tumor Response and Outcome from Pemetrexed-based Chemotherapy in Malignant Pleural Mesothelioma
Article Snippet: .. Deparaffinization, rehydration, blocking of endogenous peroxidase activity with hydrogen peroxide and staining visualization was accomplished using an ultra View Universial DAB detection kit (Ventana, Tucson, AZ, USA) on an automated staining system (BENCHMARK XT, Ventana). .. The slides were incubated for 1 hour at 37°C or at room temperature with the respective primary antibodies: rabbit polyclonal anti-FPGS antibody (dilution 1:20, Spring Bioscience, Inc., Pleasanton, CA, USA) or mouse monoclonal anti-TS antibody (dilution 1:10, Invitrogen, Inc., Carlsbad, CA, USA).

Article Title: Comparison of thyroid transcription factor-1 expression by two monoclonal antibodies in pulmonary and non-pulmonary primary tumors
Article Snippet: .. Reduction of endogenous peroxidase activity, background blocking and chromogen system was performed by using the Discovery DAB Map kit (RUO) from Ventana following the manufacturer protocol. ..

Article Title: Dynamic Contrast-Enhanced and Diffusion MRI Show Rapid and Dramatic Changes in Tumor Microenvironment in Response to Inhibition of HIF-1? Using PX-478 1
Article Snippet: .. Endogenous peroxidase activity was quenched using a hydrogen peroxide-based inhibitor (DAB Basic Detection Kit; Ventana Medical Systems, Tucson, AZ) and endogenous biotin blocked using an AB Blocking Kit (Ventana Medical Systems). .. The slides were incubated for 32 minutes at 42°C with the mouse monoclonal antihuman HIF-1α (Transduction Laboratories, Lexington, KY) at 10 g/ml.

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    Ventana Medical endogenous peroxidase activity
    Endogenous Peroxidase Activity, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 92/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endogenous peroxidase activity/product/Ventana Medical
    Average 92 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    endogenous peroxidase activity - by Bioz Stars, 2020-11
    92/100 stars
      Buy from Supplier

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