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Novocastra endogenous peroxidase activity
Endogenous Peroxidase Activity, supplied by Novocastra, used in various techniques. Bioz Stars score: 93/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/endogenous peroxidase activity/product/Novocastra
Average 93 stars, based on 75 article reviews
Price from $9.99 to $1999.99
endogenous peroxidase activity - by Bioz Stars, 2020-11
93/100 stars

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Incubation:

Article Title: Anti-tumor efficacy of fulvestrant in estrogen receptor positive gastric cancer
Article Snippet: .. After blocking endogenous peroxidase activity with 3% hydrogen peroxidase for 10 min, slides were incubated with mouse monoclonal estrogen receptor antibody (NCL-L-ER-6F11, Novocastra, Newcastle, United Kingdom) for 15 min at room temperature, at a dilution of 1:200. ..

Article Title: Fibronectin Produced by Cerebral Endothelial and Vascular Smooth Muscle Cells Contributes to Perivascular Extracellular Matrix in Late-Delayed Radiation-Induced Brain Injury
Article Snippet: .. Endogenous peroxidase activity was blocked by 10-min incubation with IHC/ISH Super Blocking Novocastra solution (Leica Biosystems). .. Secondary antibody and chromogen application were as according to manufacturer’s specifications (Bond Polymer Refine Red Detection Kit; Leica Biosystems).

Article Title: Expression of autophagy-related protein beclin-1 in malignant canine mammary tumors
Article Snippet: .. Endogenous peroxidase activity was blocked by incubation of tissue with the Novocastra Peroxidase Block for 5 min. .. The slides were then incubated for 30 min at room temperature with a primary rabbit polyclonal antibody against Beclin-1 (1: 400, Abcam, UK), estrogen receptor (1:50, clone 6 F11, Novocastra, UK), and Her2 (1:50, clone CB11, Novocastra, UK).

Article Title: Cathepsin B expression in colorectal cancer in a Middle East population: Potential value as a tumor biomarker for late disease stages
Article Snippet: .. Endogenous peroxidase activity was quenched with 3% hydrogen peroxide in distilled H2 O for 5 min, and then, the slides were washed in Tris-buffered saline (TBS) for 10 min. Non-specific binding of antibodies was blocked by incubation with protein block (Novocastra) for 5 min. .. Subsequently, the slides were incubated with human CTSB monoclonal mouse antibody (Abazyme, Cambridge, MA, USA) primary antibody (1:100) for 1 h at room temperature.

Article Title: Transferrin receptor-1 and ferritin heavy and light chains in astrocytic brain tumors: Expression and prognostic value
Article Snippet: .. After blocking of endogenous peroxidase activity by incubation in 1.5% hydrogen peroxide, the sections were incubated for 60 min with primary antibodies against TfR1 (CD71) (10F11, 1+50, NovoCastra, Newcastle, United Kingdom), FTH (ab65080, 1+800, Abcam, Cambridge, United Kingdom), or FTL (ab69090, 1+1000, Abcam). .. The antigen-antibody complex was detected using EnVision (Dako).

Article Title: Altered expression of 17-β-hydroxysteroid dehydrogenase type 2 and its prognostic significance in non-small cell lung cancer
Article Snippet: .. The sections were then incubated for 10 min at room temperature in Novocastra Peroxidase Block reagent (Leica Microsystems, Ltd.) to neutralize endogenous peroxidase activity and were treated with Novocastra Protein Block (Leica Microsystems, Ltd.) for 10 min at room temperature to reduce non-specific binding of Ab and polymer. .. Sections were then incubated over night with rabbit polyclonal anti HSD17B2 Ab at a dilution of 1:120 in a humidified chamber at 4°C.

Binding Assay:

Article Title: Cathepsin B expression in colorectal cancer in a Middle East population: Potential value as a tumor biomarker for late disease stages
Article Snippet: .. Endogenous peroxidase activity was quenched with 3% hydrogen peroxide in distilled H2 O for 5 min, and then, the slides were washed in Tris-buffered saline (TBS) for 10 min. Non-specific binding of antibodies was blocked by incubation with protein block (Novocastra) for 5 min. .. Subsequently, the slides were incubated with human CTSB monoclonal mouse antibody (Abazyme, Cambridge, MA, USA) primary antibody (1:100) for 1 h at room temperature.

Article Title: Altered expression of 17-β-hydroxysteroid dehydrogenase type 2 and its prognostic significance in non-small cell lung cancer
Article Snippet: .. The sections were then incubated for 10 min at room temperature in Novocastra Peroxidase Block reagent (Leica Microsystems, Ltd.) to neutralize endogenous peroxidase activity and were treated with Novocastra Protein Block (Leica Microsystems, Ltd.) for 10 min at room temperature to reduce non-specific binding of Ab and polymer. .. Sections were then incubated over night with rabbit polyclonal anti HSD17B2 Ab at a dilution of 1:120 in a humidified chamber at 4°C.

Activity Assay:

Article Title: Microvessel density in head and neck squamous cell carcinoma
Article Snippet: .. Endogenous peroxidase activity was blocked with a 3% hydrogen peroxide solution (Protein Block by Novolink Polymer Detection System, Novocastra). ..

Article Title: Anti-tumor efficacy of fulvestrant in estrogen receptor positive gastric cancer
Article Snippet: .. After blocking endogenous peroxidase activity with 3% hydrogen peroxidase for 10 min, slides were incubated with mouse monoclonal estrogen receptor antibody (NCL-L-ER-6F11, Novocastra, Newcastle, United Kingdom) for 15 min at room temperature, at a dilution of 1:200. ..

Article Title: Hyaluronan Mixed Esters of Butyric and Retinoic Acid Affording Myocardial Survival and Repair without Stem Cell Transplantation *
Article Snippet: .. Antigens were unmasked with citrate buffer, pH 6.0, at 120 °C, 1 atm for 21 min. After cooling and washing, endogenous peroxidase activity was neutralized using a 3% H2 O2 solution in methanol absolute for 10 min at room temperature in the dark; sections were then processed for immunohistochemistry with a non-biotin-amplified method (NovoLinkTM Polymer Detection System, Novocastra Laboratories Ltd.). .. After washing with 1× TBS, the slides were incubated with NovocastraTM protein block for 5 min in a wet chamber to reduce the nonspecific binding of primary antibody and polymer reagent and rinsed twice with 1× TBS.

Article Title: Fibronectin Produced by Cerebral Endothelial and Vascular Smooth Muscle Cells Contributes to Perivascular Extracellular Matrix in Late-Delayed Radiation-Induced Brain Injury
Article Snippet: .. Endogenous peroxidase activity was blocked by 10-min incubation with IHC/ISH Super Blocking Novocastra solution (Leica Biosystems). .. Secondary antibody and chromogen application were as according to manufacturer’s specifications (Bond Polymer Refine Red Detection Kit; Leica Biosystems).

Article Title: Expression of autophagy-related protein beclin-1 in malignant canine mammary tumors
Article Snippet: .. Endogenous peroxidase activity was blocked by incubation of tissue with the Novocastra Peroxidase Block for 5 min. .. The slides were then incubated for 30 min at room temperature with a primary rabbit polyclonal antibody against Beclin-1 (1: 400, Abcam, UK), estrogen receptor (1:50, clone 6 F11, Novocastra, UK), and Her2 (1:50, clone CB11, Novocastra, UK).

Article Title: Cathepsin B expression in colorectal cancer in a Middle East population: Potential value as a tumor biomarker for late disease stages
Article Snippet: .. Endogenous peroxidase activity was quenched with 3% hydrogen peroxide in distilled H2 O for 5 min, and then, the slides were washed in Tris-buffered saline (TBS) for 10 min. Non-specific binding of antibodies was blocked by incubation with protein block (Novocastra) for 5 min. .. Subsequently, the slides were incubated with human CTSB monoclonal mouse antibody (Abazyme, Cambridge, MA, USA) primary antibody (1:100) for 1 h at room temperature.

Article Title: Transferrin receptor-1 and ferritin heavy and light chains in astrocytic brain tumors: Expression and prognostic value
Article Snippet: .. After blocking of endogenous peroxidase activity by incubation in 1.5% hydrogen peroxide, the sections were incubated for 60 min with primary antibodies against TfR1 (CD71) (10F11, 1+50, NovoCastra, Newcastle, United Kingdom), FTH (ab65080, 1+800, Abcam, Cambridge, United Kingdom), or FTL (ab69090, 1+1000, Abcam). .. The antigen-antibody complex was detected using EnVision (Dako).

Article Title: Altered expression of 17-β-hydroxysteroid dehydrogenase type 2 and its prognostic significance in non-small cell lung cancer
Article Snippet: .. The sections were then incubated for 10 min at room temperature in Novocastra Peroxidase Block reagent (Leica Microsystems, Ltd.) to neutralize endogenous peroxidase activity and were treated with Novocastra Protein Block (Leica Microsystems, Ltd.) for 10 min at room temperature to reduce non-specific binding of Ab and polymer. .. Sections were then incubated over night with rabbit polyclonal anti HSD17B2 Ab at a dilution of 1:120 in a humidified chamber at 4°C.

Immunohistochemistry:

Article Title: Hyaluronan Mixed Esters of Butyric and Retinoic Acid Affording Myocardial Survival and Repair without Stem Cell Transplantation *
Article Snippet: .. Antigens were unmasked with citrate buffer, pH 6.0, at 120 °C, 1 atm for 21 min. After cooling and washing, endogenous peroxidase activity was neutralized using a 3% H2 O2 solution in methanol absolute for 10 min at room temperature in the dark; sections were then processed for immunohistochemistry with a non-biotin-amplified method (NovoLinkTM Polymer Detection System, Novocastra Laboratories Ltd.). .. After washing with 1× TBS, the slides were incubated with NovocastraTM protein block for 5 min in a wet chamber to reduce the nonspecific binding of primary antibody and polymer reagent and rinsed twice with 1× TBS.

Article Title: Fibronectin Produced by Cerebral Endothelial and Vascular Smooth Muscle Cells Contributes to Perivascular Extracellular Matrix in Late-Delayed Radiation-Induced Brain Injury
Article Snippet: .. Endogenous peroxidase activity was blocked by 10-min incubation with IHC/ISH Super Blocking Novocastra solution (Leica Biosystems). .. Secondary antibody and chromogen application were as according to manufacturer’s specifications (Bond Polymer Refine Red Detection Kit; Leica Biosystems).

Blocking Assay:

Article Title: Microvessel density in head and neck squamous cell carcinoma
Article Snippet: .. Endogenous peroxidase activity was blocked with a 3% hydrogen peroxide solution (Protein Block by Novolink Polymer Detection System, Novocastra). ..

Article Title: Anti-tumor efficacy of fulvestrant in estrogen receptor positive gastric cancer
Article Snippet: .. After blocking endogenous peroxidase activity with 3% hydrogen peroxidase for 10 min, slides were incubated with mouse monoclonal estrogen receptor antibody (NCL-L-ER-6F11, Novocastra, Newcastle, United Kingdom) for 15 min at room temperature, at a dilution of 1:200. ..

Article Title: Fibronectin Produced by Cerebral Endothelial and Vascular Smooth Muscle Cells Contributes to Perivascular Extracellular Matrix in Late-Delayed Radiation-Induced Brain Injury
Article Snippet: .. Endogenous peroxidase activity was blocked by 10-min incubation with IHC/ISH Super Blocking Novocastra solution (Leica Biosystems). .. Secondary antibody and chromogen application were as according to manufacturer’s specifications (Bond Polymer Refine Red Detection Kit; Leica Biosystems).

Article Title: Expression of autophagy-related protein beclin-1 in malignant canine mammary tumors
Article Snippet: .. Endogenous peroxidase activity was blocked by incubation of tissue with the Novocastra Peroxidase Block for 5 min. .. The slides were then incubated for 30 min at room temperature with a primary rabbit polyclonal antibody against Beclin-1 (1: 400, Abcam, UK), estrogen receptor (1:50, clone 6 F11, Novocastra, UK), and Her2 (1:50, clone CB11, Novocastra, UK).

Article Title: Cathepsin B expression in colorectal cancer in a Middle East population: Potential value as a tumor biomarker for late disease stages
Article Snippet: .. Endogenous peroxidase activity was quenched with 3% hydrogen peroxide in distilled H2 O for 5 min, and then, the slides were washed in Tris-buffered saline (TBS) for 10 min. Non-specific binding of antibodies was blocked by incubation with protein block (Novocastra) for 5 min. .. Subsequently, the slides were incubated with human CTSB monoclonal mouse antibody (Abazyme, Cambridge, MA, USA) primary antibody (1:100) for 1 h at room temperature.

Article Title: Transferrin receptor-1 and ferritin heavy and light chains in astrocytic brain tumors: Expression and prognostic value
Article Snippet: .. After blocking of endogenous peroxidase activity by incubation in 1.5% hydrogen peroxide, the sections were incubated for 60 min with primary antibodies against TfR1 (CD71) (10F11, 1+50, NovoCastra, Newcastle, United Kingdom), FTH (ab65080, 1+800, Abcam, Cambridge, United Kingdom), or FTL (ab69090, 1+1000, Abcam). .. The antigen-antibody complex was detected using EnVision (Dako).

Article Title: Altered expression of 17-β-hydroxysteroid dehydrogenase type 2 and its prognostic significance in non-small cell lung cancer
Article Snippet: .. The sections were then incubated for 10 min at room temperature in Novocastra Peroxidase Block reagent (Leica Microsystems, Ltd.) to neutralize endogenous peroxidase activity and were treated with Novocastra Protein Block (Leica Microsystems, Ltd.) for 10 min at room temperature to reduce non-specific binding of Ab and polymer. .. Sections were then incubated over night with rabbit polyclonal anti HSD17B2 Ab at a dilution of 1:120 in a humidified chamber at 4°C.

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    Novocastra endogenous peroxidase activity
    Endogenous Peroxidase Activity, supplied by Novocastra, used in various techniques. Bioz Stars score: 93/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endogenous peroxidase activity/product/Novocastra
    Average 93 stars, based on 75 article reviews
    Price from $9.99 to $1999.99
    endogenous peroxidase activity - by Bioz Stars, 2020-11
    93/100 stars
      Buy from Supplier

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