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Biogenex endogenous peroxidase activity
Endogenous Peroxidase Activity, supplied by Biogenex, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/endogenous peroxidase activity/product/Biogenex
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
endogenous peroxidase activity - by Bioz Stars, 2020-11
92/100 stars

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Immunohistochemistry:

Article Title: Analysis of the human thymic perivascular space during aging
Article Snippet: .. Immunohistochemical staining was performed on 4-μm FFPE sections using standard protocols, including blocking of endogenous peroxidase activity (0.6% H2 O2 in absolute methanol for 15 minutes), microwave citrate antigen retrieval (BioGenex Laboratories, San Ramon, California, USA), and blocking with diluted goat or horse serum and avidin-biotin (Vector Laboratories, Burlingame, California, USA). .. The slides were then sequentially incubated at 37°C with primary antibody, biotinylated secondary antibody, and avidin-biotin horseradish peroxidase complexes (VECTASTAIN ABC; Vector Laboratories), with intervening PBS washes.

Avidin-Biotin Assay:

Article Title: Analysis of the human thymic perivascular space during aging
Article Snippet: .. Immunohistochemical staining was performed on 4-μm FFPE sections using standard protocols, including blocking of endogenous peroxidase activity (0.6% H2 O2 in absolute methanol for 15 minutes), microwave citrate antigen retrieval (BioGenex Laboratories, San Ramon, California, USA), and blocking with diluted goat or horse serum and avidin-biotin (Vector Laboratories, Burlingame, California, USA). .. The slides were then sequentially incubated at 37°C with primary antibody, biotinylated secondary antibody, and avidin-biotin horseradish peroxidase complexes (VECTASTAIN ABC; Vector Laboratories), with intervening PBS washes.

Blocking Assay:

Article Title: CCR5 Inhibition Prevents Cardiac Dysfunction in the SIV/Macaque Model of HIV
Article Snippet: .. Peroxide block was used on sections to quench endogenous peroxidase activity followed by nonspecific background block (Power Block, Biogenex). ..

Article Title: MCC-555-induced NAG-1 expression is mediated in part by KLF4
Article Snippet: .. Endogenous peroxidase activity was blocked by 3% hydrogen peroxide, and nonspecific binding of secondary antibody was eliminated by incubation with protein block buffer (Biogenex USA, San Ramon, CA) for 30 min at room temperature. .. Slides were then incubated with primary antibodies overnight at 4°C followed by incubation with biotinylated anti-rabbit IgG and streptavidin/biotin-horseradish peroxidase complex, which was visualized by DAB staining.

Article Title: Analysis of the human thymic perivascular space during aging
Article Snippet: .. Immunohistochemical staining was performed on 4-μm FFPE sections using standard protocols, including blocking of endogenous peroxidase activity (0.6% H2 O2 in absolute methanol for 15 minutes), microwave citrate antigen retrieval (BioGenex Laboratories, San Ramon, California, USA), and blocking with diluted goat or horse serum and avidin-biotin (Vector Laboratories, Burlingame, California, USA). .. The slides were then sequentially incubated at 37°C with primary antibody, biotinylated secondary antibody, and avidin-biotin horseradish peroxidase complexes (VECTASTAIN ABC; Vector Laboratories), with intervening PBS washes.

Incubation:

Article Title: MCC-555-induced NAG-1 expression is mediated in part by KLF4
Article Snippet: .. Endogenous peroxidase activity was blocked by 3% hydrogen peroxide, and nonspecific binding of secondary antibody was eliminated by incubation with protein block buffer (Biogenex USA, San Ramon, CA) for 30 min at room temperature. .. Slides were then incubated with primary antibodies overnight at 4°C followed by incubation with biotinylated anti-rabbit IgG and streptavidin/biotin-horseradish peroxidase complex, which was visualized by DAB staining.

Formalin-fixed Paraffin-Embedded:

Article Title: Analysis of the human thymic perivascular space during aging
Article Snippet: .. Immunohistochemical staining was performed on 4-μm FFPE sections using standard protocols, including blocking of endogenous peroxidase activity (0.6% H2 O2 in absolute methanol for 15 minutes), microwave citrate antigen retrieval (BioGenex Laboratories, San Ramon, California, USA), and blocking with diluted goat or horse serum and avidin-biotin (Vector Laboratories, Burlingame, California, USA). .. The slides were then sequentially incubated at 37°C with primary antibody, biotinylated secondary antibody, and avidin-biotin horseradish peroxidase complexes (VECTASTAIN ABC; Vector Laboratories), with intervening PBS washes.

Activity Assay:

Article Title: CCR5 Inhibition Prevents Cardiac Dysfunction in the SIV/Macaque Model of HIV
Article Snippet: .. Peroxide block was used on sections to quench endogenous peroxidase activity followed by nonspecific background block (Power Block, Biogenex). ..

Article Title: Apoptosis and oxidative stress as relevant mechanisms of antitumor activity and genotoxicity of ZnO-NPs alone and in combination with N-acetyl cysteine in tumor-bearing mice
Article Snippet: .. The endogenous peroxidase activity of tissues was blocked with 3% hydrogen peroxide for 10 mins (BioGenex, San Ramon, CA, USA). .. For visualization; tissues were incubated with 100 mL horseradish peroxidase (HRP) labeled rabbit or mouse secondary antibody and then DAB chromogen was added.

Article Title: Magnetite Nanoparticles Inhibit Tumor Growth and Upregulate the Expression of P53/P16 in Ehrlich Solid Carcinoma Bearing Mice
Article Snippet: .. The endogenous peroxidase activity of tissues was blocked with 3% hydrogen peroxide for 10 min (BioGenex, San Ramon, CA, USA). .. For visualization; tissues were incubated with 100 µl horse radish peroxidase (HRP) labeled rabbit or mouse secondary antibody and then DAB chromogen was added.

Article Title: MCC-555-induced NAG-1 expression is mediated in part by KLF4
Article Snippet: .. Endogenous peroxidase activity was blocked by 3% hydrogen peroxide, and nonspecific binding of secondary antibody was eliminated by incubation with protein block buffer (Biogenex USA, San Ramon, CA) for 30 min at room temperature. .. Slides were then incubated with primary antibodies overnight at 4°C followed by incubation with biotinylated anti-rabbit IgG and streptavidin/biotin-horseradish peroxidase complex, which was visualized by DAB staining.

Article Title: INHIBITION OF APOPTOSIS EXACERBATES FATIGUE-DAMAGE TENDON INJURIES IN AN IN VIVO RAT MODEL
Article Snippet: .. Endogenous peroxidase activity was quenched using 10 % H2 O2 followed by antigen retrieval using DeCal Retrieval Solution for cleaved caspase-3 (Biogenex, Freemont, CA, USA) and proteinase-K (Dako) for all other stains. .. Non-specific binding was blocked using Rodent Block R for cleaved caspase-3 (Biocare Medical) and Dako Protein Block (Agilent) for all other stains.

Article Title: Monoclonal antibodies specific for Candida albicans Als3 that immunolabel fungal cells in vitro and in vivo and block adhesion to host surfaces
Article Snippet: .. Mounted tissue sections were dried at 60°C for 30 min, deparaffinized, hydrated to water, placed in 3% H2 O2 in methanol for 15 min to removed endogenous peroxidase activity, and rinsed two times in Supersensitive wash buffer (BioGenex HK583-5K). .. Epitope recovery consisting of treatment with 0.1% protease (Sigma P5147) diluted in Tris buffer pH 7.6 at 37°C for 20 min was followed by washing as above.

Article Title: ERG/AKR1C3/AR constitutes a feed-forward loop for AR signaling in prostate cancer cells
Article Snippet: .. Endogenous peroxidase activity was blocked by incubating in 3% H2 O2 for 20 min. Antigen retrieval was performed with Antigen Retrieval Citra Plus Solution (BioGenex, Freemont, CA) in a steamer. .. Slides were then blocked with Blocking Serum from ABC Vectastain Kit (Vector Labs, Burlingame, CA).

Article Title: Analysis of the human thymic perivascular space during aging
Article Snippet: .. Immunohistochemical staining was performed on 4-μm FFPE sections using standard protocols, including blocking of endogenous peroxidase activity (0.6% H2 O2 in absolute methanol for 15 minutes), microwave citrate antigen retrieval (BioGenex Laboratories, San Ramon, California, USA), and blocking with diluted goat or horse serum and avidin-biotin (Vector Laboratories, Burlingame, California, USA). .. The slides were then sequentially incubated at 37°C with primary antibody, biotinylated secondary antibody, and avidin-biotin horseradish peroxidase complexes (VECTASTAIN ABC; Vector Laboratories), with intervening PBS washes.

Staining:

Article Title: Analysis of the human thymic perivascular space during aging
Article Snippet: .. Immunohistochemical staining was performed on 4-μm FFPE sections using standard protocols, including blocking of endogenous peroxidase activity (0.6% H2 O2 in absolute methanol for 15 minutes), microwave citrate antigen retrieval (BioGenex Laboratories, San Ramon, California, USA), and blocking with diluted goat or horse serum and avidin-biotin (Vector Laboratories, Burlingame, California, USA). .. The slides were then sequentially incubated at 37°C with primary antibody, biotinylated secondary antibody, and avidin-biotin horseradish peroxidase complexes (VECTASTAIN ABC; Vector Laboratories), with intervening PBS washes.

Binding Assay:

Article Title: MCC-555-induced NAG-1 expression is mediated in part by KLF4
Article Snippet: .. Endogenous peroxidase activity was blocked by 3% hydrogen peroxide, and nonspecific binding of secondary antibody was eliminated by incubation with protein block buffer (Biogenex USA, San Ramon, CA) for 30 min at room temperature. .. Slides were then incubated with primary antibodies overnight at 4°C followed by incubation with biotinylated anti-rabbit IgG and streptavidin/biotin-horseradish peroxidase complex, which was visualized by DAB staining.

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    Biogenex endogenous peroxidase activity
    Endogenous Peroxidase Activity, supplied by Biogenex, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endogenous peroxidase activity/product/Biogenex
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    endogenous peroxidase activity - by Bioz Stars, 2020-11
    92/100 stars
      Buy from Supplier

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