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Biocare Medical endogenous peroxidase activity
Endogenous Peroxidase Activity, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 92/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/endogenous peroxidase activity/product/Biocare Medical
Average 92 stars, based on 111 article reviews
Price from $9.99 to $1999.99
endogenous peroxidase activity - by Bioz Stars, 2020-11
92/100 stars

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Immunohistochemistry:

Article Title: Molecular Mechanism of Altered Ezetimibe Disposition in Nonalcoholic Steatohepatitis
Article Snippet: .. Endogenous peroxidase activity was blocked with 0.3% (v/v) H2 O2 in methanol for 20 min. Immunohistochemical staining for Abcb1 was performed with a MACH3 staining kit (Biocare Medical, Concord, CA) per the manufacturer's protocol. .. Samples were incubated in a primary antibody (antibodies described above) solution overnight at 4°C.

Blocking Assay:

Article Title: Amyloidosis in a Captive Zebra Finch (Taeniopygia guttata) Research Colony
Article Snippet: .. For this study, endogenous peroxidase activity in deparaffinated sections was blocked by incubating the slides for 5 min in a commercial reagent (Intellipath FLX blocking reagent, Biocare Medical, Concord, CA). .. Sections were subsequently incubated for 60 min at room temperature with the rabbit antisera (dilution, 1:600), rinsed in wash buffer (PBS Plus, BioCare Medical), and incubated for 20 min with an antirabbit secondary detection system (catalog no. RMR622, BioCare Medical).

Article Title: Rapamycin Partially Mimics the Anticancer Effects of Calorie Restriction in a Murine Model of Pancreatic Cancer
Article Snippet: .. Endogenous peroxidase activity was quenched with 3% hydrogen peroxide for 10 min. Nonspecific binding was blocked with Biocare blocking reagent (Biocare Medical, Concord, CA) for 30 min at RT, then sections were incubated with primary antibody diluted in blocking buffer. .. The following primary antibodies (source, dilution and incubation conditions presented parenthetically) were used: Ki-67 (Dako, Carpenteria, CA; 1:200, 4°C overnight); phospho-IGF1R Tyr1131 and IGF1R (Cell Signaling, Danvers, MA; 1:50, 4°C overnight); phospho-AktSer473 (Santa Cruz Biotechnology, Santa Cruz, CA; 1:50, 1 h RT); Akt (Cell Signaling; 1:100; 4°C overnight) phospho-GSK-3βSer9 (Santa Cruz Biotechnology; 1:50, 1 h RT); GSK-3β (Millipore; 1:200; 1 h RT); phospho-ERKThr202/Tyr204 (Cell Signaling; 1:100; 1 h RT); ERK (Cell Signaling; 1:25; 1 h RT); phospho-Stat3Tyr705 (Cell Signaling; 1:50; 4°C overnight); phospho-Stat3Ser727 (Cell Signaling; 1:50, 4°C overnight); STAT3 (Cell Signaling; 1:100; 1 h RT); phospho-mTORSer2448 (Cell Signaling; 1:50, 4°C overnight); mTOR (Cell Signaling; 1:100; 1 h RT); phospho-S6 ribosomal protein(Ser235/236) (Cell Signaling; 1:50, 1 h RT); S6 (Cell Signaling; 1:100; 4°C overnight) and cyclin D1 (Santa Cruz Biotechnology; 1:500, 2h RT).

Incubation:

Article Title: Establishing 177Lu-PSMA-617 Radioligand Therapy in a Syngeneic Model of Murine Prostate Cancer
Article Snippet: .. Endogenous peroxidase activity was blocked with 3% hydrogen peroxide in methanol for 10 min. Heat-induced antigen retrieval was performed for all sections in 0.01 M citrate buffer, pH 6.0, using a Biocare decloaker (Biocare Medical) at 95°C for 25 min. Primary rabbit polyclonal antigamma H2A.X (phospho S139) IgG (ab2893; Abcam) was added at 1:500 dilution in bovine serum albumin and incubated overnight at 4°C. .. Secondary staining was performed using the Dakocytomation Envision System labeled polymer horseradish peroxidase antirabbit (K4003; DakoCytomation, Carpinteria) and visualized with the diaminobenzidine reaction (#BDB2004 L; Biocare Medical) according to the manufacturer’s instructions.

Article Title: Direct and Indirect Roles of Cyclin-dependent Kinase 5 as an Upstream Regulator in the c-Jun NH2-Terminal Kinase Cascade: Relevance to Neurotoxic Insults in Alzheimer's Disease
Article Snippet: .. Briefly, slides were immersed in xylene and hydrated through graded ethanol solutions, and endogenous peroxidase activity was eliminated by incubation in 3% hydrogen peroxide for 30 min. For antigen retrieval, each slide was heated in a pressure cooker in rodent decloaker buffer (Biocare Medical, Concord, CA). .. To reduce nonspecific binding, sections were incubated for 30 min in 10% normal goat serum (NGS) in Tris-buffered saline (TBS; 50 mM Tris-HCl, and 150 mM NaCl, pH 7.6).

Article Title: Molecular Imaging of Nuclear Factor-kappaB in Urinary Bladder as a Primary Regulator of Inflammatory Response
Article Snippet: .. The sections were blocked with PEROXIdazed-1 for endogenous peroxidase activity and BACKGROUNDsniper (both from Biocare Medical) to reduce nonspecific background staining, and incubated in primary monoclonal antibodies for 1 h at RT. .. Control sections were incubated with isotype-matched IgG normal goat serum.

Article Title: Rapamycin Partially Mimics the Anticancer Effects of Calorie Restriction in a Murine Model of Pancreatic Cancer
Article Snippet: .. Endogenous peroxidase activity was quenched with 3% hydrogen peroxide for 10 min. Nonspecific binding was blocked with Biocare blocking reagent (Biocare Medical, Concord, CA) for 30 min at RT, then sections were incubated with primary antibody diluted in blocking buffer. .. The following primary antibodies (source, dilution and incubation conditions presented parenthetically) were used: Ki-67 (Dako, Carpenteria, CA; 1:200, 4°C overnight); phospho-IGF1R Tyr1131 and IGF1R (Cell Signaling, Danvers, MA; 1:50, 4°C overnight); phospho-AktSer473 (Santa Cruz Biotechnology, Santa Cruz, CA; 1:50, 1 h RT); Akt (Cell Signaling; 1:100; 4°C overnight) phospho-GSK-3βSer9 (Santa Cruz Biotechnology; 1:50, 1 h RT); GSK-3β (Millipore; 1:200; 1 h RT); phospho-ERKThr202/Tyr204 (Cell Signaling; 1:100; 1 h RT); ERK (Cell Signaling; 1:25; 1 h RT); phospho-Stat3Tyr705 (Cell Signaling; 1:50; 4°C overnight); phospho-Stat3Ser727 (Cell Signaling; 1:50, 4°C overnight); STAT3 (Cell Signaling; 1:100; 1 h RT); phospho-mTORSer2448 (Cell Signaling; 1:50, 4°C overnight); mTOR (Cell Signaling; 1:100; 1 h RT); phospho-S6 ribosomal protein(Ser235/236) (Cell Signaling; 1:50, 1 h RT); S6 (Cell Signaling; 1:100; 4°C overnight) and cyclin D1 (Santa Cruz Biotechnology; 1:500, 2h RT).

Article Title: Epigenetic Silencing of miRNA-34a in Human Cholangiocarcinoma via EZH2 and DNA Methylation
Article Snippet: .. After washing the sections with deionized water, endogenous peroxidase activity was quenched by incubation with 3% H2 O2 for 5 minutes, followed by buffer wash (TWB945; Biocare Medical). .. The sections were incubated with avidin (AB972; Biocare Medical, Pike Lane Concord, CA) for 10 minutes and biotin for 10 minutes.

Activity Assay:

Article Title: Amyloidosis in a Captive Zebra Finch (Taeniopygia guttata) Research Colony
Article Snippet: .. For this study, endogenous peroxidase activity in deparaffinated sections was blocked by incubating the slides for 5 min in a commercial reagent (Intellipath FLX blocking reagent, Biocare Medical, Concord, CA). .. Sections were subsequently incubated for 60 min at room temperature with the rabbit antisera (dilution, 1:600), rinsed in wash buffer (PBS Plus, BioCare Medical), and incubated for 20 min with an antirabbit secondary detection system (catalog no. RMR622, BioCare Medical).

Article Title: Establishing 177Lu-PSMA-617 Radioligand Therapy in a Syngeneic Model of Murine Prostate Cancer
Article Snippet: .. Endogenous peroxidase activity was blocked with 3% hydrogen peroxide in methanol for 10 min. Heat-induced antigen retrieval was performed for all sections in 0.01 M citrate buffer, pH 6.0, using a Biocare decloaker (Biocare Medical) at 95°C for 25 min. Primary rabbit polyclonal antigamma H2A.X (phospho S139) IgG (ab2893; Abcam) was added at 1:500 dilution in bovine serum albumin and incubated overnight at 4°C. .. Secondary staining was performed using the Dakocytomation Envision System labeled polymer horseradish peroxidase antirabbit (K4003; DakoCytomation, Carpinteria) and visualized with the diaminobenzidine reaction (#BDB2004 L; Biocare Medical) according to the manufacturer’s instructions.

Article Title: Direct and Indirect Roles of Cyclin-dependent Kinase 5 as an Upstream Regulator in the c-Jun NH2-Terminal Kinase Cascade: Relevance to Neurotoxic Insults in Alzheimer's Disease
Article Snippet: .. Briefly, slides were immersed in xylene and hydrated through graded ethanol solutions, and endogenous peroxidase activity was eliminated by incubation in 3% hydrogen peroxide for 30 min. For antigen retrieval, each slide was heated in a pressure cooker in rodent decloaker buffer (Biocare Medical, Concord, CA). .. To reduce nonspecific binding, sections were incubated for 30 min in 10% normal goat serum (NGS) in Tris-buffered saline (TBS; 50 mM Tris-HCl, and 150 mM NaCl, pH 7.6).

Article Title: Molecular Imaging of Nuclear Factor-kappaB in Urinary Bladder as a Primary Regulator of Inflammatory Response
Article Snippet: .. The sections were blocked with PEROXIdazed-1 for endogenous peroxidase activity and BACKGROUNDsniper (both from Biocare Medical) to reduce nonspecific background staining, and incubated in primary monoclonal antibodies for 1 h at RT. .. Control sections were incubated with isotype-matched IgG normal goat serum.

Article Title: Mast cells and dendritic cells form synapses that facilitate antigen transfer for T cell activation
Article Snippet: .. Sections were treated with 3% H2 O2 to inactivate the endogenous peroxidase activity, and antigen retrieval was accomplished by immersing slides in 10 mM citrate solution, pH 6.0, and boiling them for 10 min at 120°C (20–25 p.s.i.) in a Decloaking Chamber (Biocare Medical). ..

Article Title: Rapamycin Partially Mimics the Anticancer Effects of Calorie Restriction in a Murine Model of Pancreatic Cancer
Article Snippet: .. Endogenous peroxidase activity was quenched with 3% hydrogen peroxide for 10 min. Nonspecific binding was blocked with Biocare blocking reagent (Biocare Medical, Concord, CA) for 30 min at RT, then sections were incubated with primary antibody diluted in blocking buffer. .. The following primary antibodies (source, dilution and incubation conditions presented parenthetically) were used: Ki-67 (Dako, Carpenteria, CA; 1:200, 4°C overnight); phospho-IGF1R Tyr1131 and IGF1R (Cell Signaling, Danvers, MA; 1:50, 4°C overnight); phospho-AktSer473 (Santa Cruz Biotechnology, Santa Cruz, CA; 1:50, 1 h RT); Akt (Cell Signaling; 1:100; 4°C overnight) phospho-GSK-3βSer9 (Santa Cruz Biotechnology; 1:50, 1 h RT); GSK-3β (Millipore; 1:200; 1 h RT); phospho-ERKThr202/Tyr204 (Cell Signaling; 1:100; 1 h RT); ERK (Cell Signaling; 1:25; 1 h RT); phospho-Stat3Tyr705 (Cell Signaling; 1:50; 4°C overnight); phospho-Stat3Ser727 (Cell Signaling; 1:50, 4°C overnight); STAT3 (Cell Signaling; 1:100; 1 h RT); phospho-mTORSer2448 (Cell Signaling; 1:50, 4°C overnight); mTOR (Cell Signaling; 1:100; 1 h RT); phospho-S6 ribosomal protein(Ser235/236) (Cell Signaling; 1:50, 1 h RT); S6 (Cell Signaling; 1:100; 4°C overnight) and cyclin D1 (Santa Cruz Biotechnology; 1:500, 2h RT).

Article Title: Epigenetic Silencing of miRNA-34a in Human Cholangiocarcinoma via EZH2 and DNA Methylation
Article Snippet: .. After washing the sections with deionized water, endogenous peroxidase activity was quenched by incubation with 3% H2 O2 for 5 minutes, followed by buffer wash (TWB945; Biocare Medical). .. The sections were incubated with avidin (AB972; Biocare Medical, Pike Lane Concord, CA) for 10 minutes and biotin for 10 minutes.

Article Title: Molecular Mechanism of Altered Ezetimibe Disposition in Nonalcoholic Steatohepatitis
Article Snippet: .. Endogenous peroxidase activity was blocked with 0.3% (v/v) H2 O2 in methanol for 20 min. Immunohistochemical staining for Abcb1 was performed with a MACH3 staining kit (Biocare Medical, Concord, CA) per the manufacturer's protocol. .. Samples were incubated in a primary antibody (antibodies described above) solution overnight at 4°C.

Staining:

Article Title: Molecular Imaging of Nuclear Factor-kappaB in Urinary Bladder as a Primary Regulator of Inflammatory Response
Article Snippet: .. The sections were blocked with PEROXIdazed-1 for endogenous peroxidase activity and BACKGROUNDsniper (both from Biocare Medical) to reduce nonspecific background staining, and incubated in primary monoclonal antibodies for 1 h at RT. .. Control sections were incubated with isotype-matched IgG normal goat serum.

Article Title: Molecular Mechanism of Altered Ezetimibe Disposition in Nonalcoholic Steatohepatitis
Article Snippet: .. Endogenous peroxidase activity was blocked with 0.3% (v/v) H2 O2 in methanol for 20 min. Immunohistochemical staining for Abcb1 was performed with a MACH3 staining kit (Biocare Medical, Concord, CA) per the manufacturer's protocol. .. Samples were incubated in a primary antibody (antibodies described above) solution overnight at 4°C.

Binding Assay:

Article Title: Rapamycin Partially Mimics the Anticancer Effects of Calorie Restriction in a Murine Model of Pancreatic Cancer
Article Snippet: .. Endogenous peroxidase activity was quenched with 3% hydrogen peroxide for 10 min. Nonspecific binding was blocked with Biocare blocking reagent (Biocare Medical, Concord, CA) for 30 min at RT, then sections were incubated with primary antibody diluted in blocking buffer. .. The following primary antibodies (source, dilution and incubation conditions presented parenthetically) were used: Ki-67 (Dako, Carpenteria, CA; 1:200, 4°C overnight); phospho-IGF1R Tyr1131 and IGF1R (Cell Signaling, Danvers, MA; 1:50, 4°C overnight); phospho-AktSer473 (Santa Cruz Biotechnology, Santa Cruz, CA; 1:50, 1 h RT); Akt (Cell Signaling; 1:100; 4°C overnight) phospho-GSK-3βSer9 (Santa Cruz Biotechnology; 1:50, 1 h RT); GSK-3β (Millipore; 1:200; 1 h RT); phospho-ERKThr202/Tyr204 (Cell Signaling; 1:100; 1 h RT); ERK (Cell Signaling; 1:25; 1 h RT); phospho-Stat3Tyr705 (Cell Signaling; 1:50; 4°C overnight); phospho-Stat3Ser727 (Cell Signaling; 1:50, 4°C overnight); STAT3 (Cell Signaling; 1:100; 1 h RT); phospho-mTORSer2448 (Cell Signaling; 1:50, 4°C overnight); mTOR (Cell Signaling; 1:100; 1 h RT); phospho-S6 ribosomal protein(Ser235/236) (Cell Signaling; 1:50, 1 h RT); S6 (Cell Signaling; 1:100; 4°C overnight) and cyclin D1 (Santa Cruz Biotechnology; 1:500, 2h RT).

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    Biocare Medical endogenous peroxidase activity
    Endogenous Peroxidase Activity, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 92/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endogenous peroxidase activity/product/Biocare Medical
    Average 92 stars, based on 111 article reviews
    Price from $9.99 to $1999.99
    endogenous peroxidase activity - by Bioz Stars, 2020-11
    92/100 stars
      Buy from Supplier

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