endogenous peroxidase activity  (Beyotime)

 
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    Name:
    Endogenous Peroxidase Blocking Buffer
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    Catalog Number:
    p0100a
    Price:
    55.0
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    Structured Review

    Beyotime endogenous peroxidase activity

    https://www.bioz.com/result/endogenous peroxidase activity/product/Beyotime
    Average 99 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    endogenous peroxidase activity - by Bioz Stars, 2020-11
    99/100 stars

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    Related Articles

    TUNEL Assay:

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes protect against myocardial infarction by promoting autophagy
    Article Snippet: .. TUNEL staining was performed by incubation with Protease K solution at 37°C for 30 min, incubation with TdT and dUTP mixture (2:29) at 37°C for 2 h, blocking of endogenous peroxidase with 3% hydrogen peroxide solution in methanol in the dark for 15 min and incubation with converter-POD at 37°C for 30 min. mounting with Antifade Mounting Medium (cat. no. P0126; Beyotime Institute of Biotechnology) and observed using an inverted fluorescence microscope (TE2000-E; Nikon Corporation) with a ×400 magnification for at least 6 fields. .. 2,3,5-triphenyltetrazolium chloride (TTC) staining was performed by immersion in 1% TTC at RT for 15 min. For Masson staining, the tissue slides were stained with Ponceau S acid fuchsin at RT for 5–10 min, followed by incubation with a molybdophosphoric acid solution at RT for 3 min and staining with aniline blue at RT for 5 min, and finally observed with a camera (EOS 80D; Canon, Inc.).

    Immunohistochemistry:

    Article Title: Allogenic Tendon-Autologous Cartilage Cells Transplantation Enhances Adhesive/Growth Ability and Promotes Chondrogenesis in a Rabbit Model of Glenoid Labrum Damage
    Article Snippet: .. Immunohistochemistry assay Cartilage cells were fixed with 4% paraformaldehyde (Sangon Biotech., Shanghai, China) for 15 min and the endogenous peroxidase was inactivated with 3% hydrogen peroxide (Beyotime Biotech, Shanghai, China) at room temperature for 5 min. .. The cells were then blocked with 5% bovine serum albumin (BSA, Gibco BRL.

    Article Title: In Vivo Tracking and Comparison of the Therapeutic Effects of MSCs and HSCs for Liver Injury
    Article Snippet: .. For immunohistochemical studies, after microwave-based antigen retrieval, the sections were treated with 0.3% H2 O2 in PBS to quench the endogenous peroxidase and then incubated with 5% goat serum (Beyotime, China) to block the non-specific sites. .. Monoclonal primary antibodies against mouse Ki-67 (1∶100, Abcam, USA) were applied when incubating at 4°C for 12 h, followed by incubation with the corresponding secondary antibodies (Beyotime, China) at 37°C for 30 min.

    Fluorescence:

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes protect against myocardial infarction by promoting autophagy
    Article Snippet: .. TUNEL staining was performed by incubation with Protease K solution at 37°C for 30 min, incubation with TdT and dUTP mixture (2:29) at 37°C for 2 h, blocking of endogenous peroxidase with 3% hydrogen peroxide solution in methanol in the dark for 15 min and incubation with converter-POD at 37°C for 30 min. mounting with Antifade Mounting Medium (cat. no. P0126; Beyotime Institute of Biotechnology) and observed using an inverted fluorescence microscope (TE2000-E; Nikon Corporation) with a ×400 magnification for at least 6 fields. .. 2,3,5-triphenyltetrazolium chloride (TTC) staining was performed by immersion in 1% TTC at RT for 15 min. For Masson staining, the tissue slides were stained with Ponceau S acid fuchsin at RT for 5–10 min, followed by incubation with a molybdophosphoric acid solution at RT for 3 min and staining with aniline blue at RT for 5 min, and finally observed with a camera (EOS 80D; Canon, Inc.).

    Blocking Assay:

    Article Title: MicroRNA-422a functions as a tumor suppressor in glioma by regulating the Wnt/β-catenin signaling pathway via RPN2
    Article Snippet: .. Subsequently, these sections were incubated with 3% hydrogen peroxide for 20 min to block endogenous peroxidase. .. Following antigen retrieval, the slides were blocked with 10% normal goat serum (Beijing Solarbio Science & Technology Co., Ltd.) for 30 min and then incubated at 4°C overnight with appropriate primary antibody.

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes protect against myocardial infarction by promoting autophagy
    Article Snippet: .. TUNEL staining was performed by incubation with Protease K solution at 37°C for 30 min, incubation with TdT and dUTP mixture (2:29) at 37°C for 2 h, blocking of endogenous peroxidase with 3% hydrogen peroxide solution in methanol in the dark for 15 min and incubation with converter-POD at 37°C for 30 min. mounting with Antifade Mounting Medium (cat. no. P0126; Beyotime Institute of Biotechnology) and observed using an inverted fluorescence microscope (TE2000-E; Nikon Corporation) with a ×400 magnification for at least 6 fields. .. 2,3,5-triphenyltetrazolium chloride (TTC) staining was performed by immersion in 1% TTC at RT for 15 min. For Masson staining, the tissue slides were stained with Ponceau S acid fuchsin at RT for 5–10 min, followed by incubation with a molybdophosphoric acid solution at RT for 3 min and staining with aniline blue at RT for 5 min, and finally observed with a camera (EOS 80D; Canon, Inc.).

    Article Title: Capsaicin and dihydrocapsaicin induce apoptosis in human glioma cells via ROS and Ca2+-mediated mitochondrial pathway
    Article Snippet: .. Endogenous peroxidase activity was blocked with Immunol Staining Blocking buffer (Beyotime Institute of Biotechnology) for 1 h at room temperature to reduce nonspecific background staining. .. Subsequently, cyto C primary antibody diluted 1:1,000 in Immunol Staining Primary Antibody Dilution buffer (Beyotime Institute of Biotechnology) was added to the slides, incubated for 1 h at room temperature, then washed in PBS.

    Article Title: In Vivo Tracking and Comparison of the Therapeutic Effects of MSCs and HSCs for Liver Injury
    Article Snippet: .. For immunohistochemical studies, after microwave-based antigen retrieval, the sections were treated with 0.3% H2 O2 in PBS to quench the endogenous peroxidase and then incubated with 5% goat serum (Beyotime, China) to block the non-specific sites. .. Monoclonal primary antibodies against mouse Ki-67 (1∶100, Abcam, USA) were applied when incubating at 4°C for 12 h, followed by incubation with the corresponding secondary antibodies (Beyotime, China) at 37°C for 30 min.

    Article Title: MicroRNA-29b inhibits TGF-β1-induced fibrosis via regulation of the TGF-β1/Smad pathway in primary human endometrial stromal cells
    Article Snippet: .. Cells were permeabilized with 0.5% Triton X-100 (Shanghai Solarbio Bioscience & Technology Co., Ltd., Shanghai, China) for 10 min, rinsed with PBS three times, incubated with 3% H2 O2 for 15 min to remove endogenous peroxidase and incubated for 30 min with 5% BSA for antigen blocking (Beyotime Institute of Biotechnology). .. Primary antibodies targeting mouse monoclonal cytokeratin 18 (cat. no. sc-32329; 1:400 dilution; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and mouse monoclonal vimentin (cat. no. sc-373717; 1:400 dilution; Santa Cruz Biotechnology, Inc.) were incubated with cells for 2 h at room temperature followed by incubation with peroxidase-conjugated goat anti-mouse IgG secondary antibody (cat. no. ZDR-5307; ZSGB-BIO, Beijing, China) for 40 min at room temperature, as previously described ( ).

    Incubation:

    Article Title: Downregulation of ZNF365 by methylation predicts poor prognosis in patients with colorectal cancer by decreasing phospho-p53 (Ser15) expression
    Article Snippet: .. After cooling to room temperature, the tissue sections were blocked with 3% hydrogen peroxidase-methanol solution for 30 min to inhibit endogenous peroxidase activity, and subsequently incubated in preimmunized goat serum (cat. no. C0265; Beyotime Institute of Biotechnology) for 30 min, both at room temperature. .. Tissue sections were incubated with rabbit polyclonal IgG primary antibody directed against ZNF365 (1:50; cat. no. HPA052446; Atlas Antibodies) overnight at 4°C.

    Article Title: MicroRNA-422a functions as a tumor suppressor in glioma by regulating the Wnt/β-catenin signaling pathway via RPN2
    Article Snippet: .. Subsequently, these sections were incubated with 3% hydrogen peroxide for 20 min to block endogenous peroxidase. .. Following antigen retrieval, the slides were blocked with 10% normal goat serum (Beijing Solarbio Science & Technology Co., Ltd.) for 30 min and then incubated at 4°C overnight with appropriate primary antibody.

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes protect against myocardial infarction by promoting autophagy
    Article Snippet: .. TUNEL staining was performed by incubation with Protease K solution at 37°C for 30 min, incubation with TdT and dUTP mixture (2:29) at 37°C for 2 h, blocking of endogenous peroxidase with 3% hydrogen peroxide solution in methanol in the dark for 15 min and incubation with converter-POD at 37°C for 30 min. mounting with Antifade Mounting Medium (cat. no. P0126; Beyotime Institute of Biotechnology) and observed using an inverted fluorescence microscope (TE2000-E; Nikon Corporation) with a ×400 magnification for at least 6 fields. .. 2,3,5-triphenyltetrazolium chloride (TTC) staining was performed by immersion in 1% TTC at RT for 15 min. For Masson staining, the tissue slides were stained with Ponceau S acid fuchsin at RT for 5–10 min, followed by incubation with a molybdophosphoric acid solution at RT for 3 min and staining with aniline blue at RT for 5 min, and finally observed with a camera (EOS 80D; Canon, Inc.).

    Article Title: In Vivo Tracking and Comparison of the Therapeutic Effects of MSCs and HSCs for Liver Injury
    Article Snippet: .. For immunohistochemical studies, after microwave-based antigen retrieval, the sections were treated with 0.3% H2 O2 in PBS to quench the endogenous peroxidase and then incubated with 5% goat serum (Beyotime, China) to block the non-specific sites. .. Monoclonal primary antibodies against mouse Ki-67 (1∶100, Abcam, USA) were applied when incubating at 4°C for 12 h, followed by incubation with the corresponding secondary antibodies (Beyotime, China) at 37°C for 30 min.

    Article Title: Advanced oxidation protein products induce intestine epithelial cell death through a redox-dependent, c-jun N-terminal kinase and poly (ADP-ribose) polymerase-1-mediated pathway
    Article Snippet: .. After inactivating endogenous peroxidase, sections were incubated in TdT buffer containing FITC-conjugated dUTP at 37 °C for 60 min. Morphological nuclear changes were observed by counterstaining with DAPI (Beyotime). .. The sections were analyzed under a confocal microscope (Carl Zeiss, Inc., Oberkochen, Germany).

    Article Title: MicroRNA-29b inhibits TGF-β1-induced fibrosis via regulation of the TGF-β1/Smad pathway in primary human endometrial stromal cells
    Article Snippet: .. Cells were permeabilized with 0.5% Triton X-100 (Shanghai Solarbio Bioscience & Technology Co., Ltd., Shanghai, China) for 10 min, rinsed with PBS three times, incubated with 3% H2 O2 for 15 min to remove endogenous peroxidase and incubated for 30 min with 5% BSA for antigen blocking (Beyotime Institute of Biotechnology). .. Primary antibodies targeting mouse monoclonal cytokeratin 18 (cat. no. sc-32329; 1:400 dilution; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and mouse monoclonal vimentin (cat. no. sc-373717; 1:400 dilution; Santa Cruz Biotechnology, Inc.) were incubated with cells for 2 h at room temperature followed by incubation with peroxidase-conjugated goat anti-mouse IgG secondary antibody (cat. no. ZDR-5307; ZSGB-BIO, Beijing, China) for 40 min at room temperature, as previously described ( ).

    Activity Assay:

    Article Title: Downregulation of ZNF365 by methylation predicts poor prognosis in patients with colorectal cancer by decreasing phospho-p53 (Ser15) expression
    Article Snippet: .. After cooling to room temperature, the tissue sections were blocked with 3% hydrogen peroxidase-methanol solution for 30 min to inhibit endogenous peroxidase activity, and subsequently incubated in preimmunized goat serum (cat. no. C0265; Beyotime Institute of Biotechnology) for 30 min, both at room temperature. .. Tissue sections were incubated with rabbit polyclonal IgG primary antibody directed against ZNF365 (1:50; cat. no. HPA052446; Atlas Antibodies) overnight at 4°C.

    Article Title: Capsaicin and dihydrocapsaicin induce apoptosis in human glioma cells via ROS and Ca2+-mediated mitochondrial pathway
    Article Snippet: .. Endogenous peroxidase activity was blocked with Immunol Staining Blocking buffer (Beyotime Institute of Biotechnology) for 1 h at room temperature to reduce nonspecific background staining. .. Subsequently, cyto C primary antibody diluted 1:1,000 in Immunol Staining Primary Antibody Dilution buffer (Beyotime Institute of Biotechnology) was added to the slides, incubated for 1 h at room temperature, then washed in PBS.

    Microscopy:

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes protect against myocardial infarction by promoting autophagy
    Article Snippet: .. TUNEL staining was performed by incubation with Protease K solution at 37°C for 30 min, incubation with TdT and dUTP mixture (2:29) at 37°C for 2 h, blocking of endogenous peroxidase with 3% hydrogen peroxide solution in methanol in the dark for 15 min and incubation with converter-POD at 37°C for 30 min. mounting with Antifade Mounting Medium (cat. no. P0126; Beyotime Institute of Biotechnology) and observed using an inverted fluorescence microscope (TE2000-E; Nikon Corporation) with a ×400 magnification for at least 6 fields. .. 2,3,5-triphenyltetrazolium chloride (TTC) staining was performed by immersion in 1% TTC at RT for 15 min. For Masson staining, the tissue slides were stained with Ponceau S acid fuchsin at RT for 5–10 min, followed by incubation with a molybdophosphoric acid solution at RT for 3 min and staining with aniline blue at RT for 5 min, and finally observed with a camera (EOS 80D; Canon, Inc.).

    Staining:

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes protect against myocardial infarction by promoting autophagy
    Article Snippet: .. TUNEL staining was performed by incubation with Protease K solution at 37°C for 30 min, incubation with TdT and dUTP mixture (2:29) at 37°C for 2 h, blocking of endogenous peroxidase with 3% hydrogen peroxide solution in methanol in the dark for 15 min and incubation with converter-POD at 37°C for 30 min. mounting with Antifade Mounting Medium (cat. no. P0126; Beyotime Institute of Biotechnology) and observed using an inverted fluorescence microscope (TE2000-E; Nikon Corporation) with a ×400 magnification for at least 6 fields. .. 2,3,5-triphenyltetrazolium chloride (TTC) staining was performed by immersion in 1% TTC at RT for 15 min. For Masson staining, the tissue slides were stained with Ponceau S acid fuchsin at RT for 5–10 min, followed by incubation with a molybdophosphoric acid solution at RT for 3 min and staining with aniline blue at RT for 5 min, and finally observed with a camera (EOS 80D; Canon, Inc.).

    Article Title: Capsaicin and dihydrocapsaicin induce apoptosis in human glioma cells via ROS and Ca2+-mediated mitochondrial pathway
    Article Snippet: .. Endogenous peroxidase activity was blocked with Immunol Staining Blocking buffer (Beyotime Institute of Biotechnology) for 1 h at room temperature to reduce nonspecific background staining. .. Subsequently, cyto C primary antibody diluted 1:1,000 in Immunol Staining Primary Antibody Dilution buffer (Beyotime Institute of Biotechnology) was added to the slides, incubated for 1 h at room temperature, then washed in PBS.

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  • News
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  • 99
    Beyotime endogenous peroxidase activity
    Endogenous Peroxidase Activity, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endogenous peroxidase activity/product/Beyotime
    Average 99 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    endogenous peroxidase activity - by Bioz Stars, 2020-11
    99/100 stars
      Buy from Supplier

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