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Agilent technologies endogenous peroxidase activity
Endogenous Peroxidase Activity, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 1237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/endogenous peroxidase activity/product/Agilent technologies
Average 94 stars, based on 1237 article reviews
Price from $9.99 to $1999.99
endogenous peroxidase activity - by Bioz Stars, 2020-11
94/100 stars

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Immunostaining:

Article Title: Mitotic activity index and CD25+ lymphocytes predict risk of stage progression in non-muscle invasive bladder cancer
Article Snippet: .. Endogenous peroxidase activity was inactivated by the incubation of tissue sections in the peroxidase-blocking reagent (DAKO, Glostrup, Denmark; S2001) for 10 min. Immunostaining of CD4+, CD8+, CD25+ T lymphocyte subsets, CD138+ plasma cells and proliferation marker Ki67 was performed using an Autostainer (DAKO, Glostrup, Denmark). .. The tissue sections were incubated with the monoclonal antibodies using the following dilutions: CD4 (Novocastra, Newcastle upon Tyne, UK; clone 1F6, 1:20); CD8 (DAKO, Glostrup, Denmark; clone C8/144B, 1:50); CD25 (Novocastra, Newcastle upon Tyne, UK; clone 4C9, 1:150); CD138 (Serotec, Kidlington, UK; clone B‑B4, 1:200) and Ki67 (DAKO, Glostrup, Denmark; clone MIB-1, 1:100).

Incubation:

Article Title: Differential CD4+ T-Lymphocyte Apoptosis and Bystander T-Cell Activation in Rhesus Macaques and Sooty Mangabeys during Acute Simian Immunodeficiency Virus Infection ▿
Article Snippet: .. Endogenous peroxidase activity was blocked by incubation in 3% H2 O2 , and antigen retrieval was accomplished by microwaving sections for 20 min in citrate buffer (Dako Corp., Carpinteria, CA). .. Tissue sections were treated for nonspecific protein binding (Protein Block; Dako) and then sequentially incubated for 30 min at room temperature with polyclonal rabbit anti-human cleaved caspase-3 (Cell Signaling Technology, Inc., Boston, MA) followed by biotinylated goat anti-rabbit IgG.

Article Title: Mitotic activity index and CD25+ lymphocytes predict risk of stage progression in non-muscle invasive bladder cancer
Article Snippet: .. Endogenous peroxidase activity was inactivated by the incubation of tissue sections in the peroxidase-blocking reagent (DAKO, Glostrup, Denmark; S2001) for 10 min. Immunostaining of CD4+, CD8+, CD25+ T lymphocyte subsets, CD138+ plasma cells and proliferation marker Ki67 was performed using an Autostainer (DAKO, Glostrup, Denmark). .. The tissue sections were incubated with the monoclonal antibodies using the following dilutions: CD4 (Novocastra, Newcastle upon Tyne, UK; clone 1F6, 1:20); CD8 (DAKO, Glostrup, Denmark; clone C8/144B, 1:50); CD25 (Novocastra, Newcastle upon Tyne, UK; clone 4C9, 1:150); CD138 (Serotec, Kidlington, UK; clone B‑B4, 1:200) and Ki67 (DAKO, Glostrup, Denmark; clone MIB-1, 1:100).

Article Title: SARS spike protein induces phenotypic conversion of human B cells to macrophage-like cells
Article Snippet: .. Following blockage of the endogenous peroxidase activity and nonspecific antibody binding sites with 3% H2 O2 /0.2% NaN3 [15 min, room temperature (RT)] and 5% goat serum (10 min, RT), respectively, slides were incubated with monoclonal antibodies specific to human CD-68 (DAKO, Glostrup, Denmark) at RT for 90 min, before further treatment with LSAB2 (DAKO). .. Chromogenic development was processed with peroxidase-conjugated streptavidin and aminoethylcarbazole (Sigma, St. Louis, MO) solution.

Article Title: Molecular and immunohistochemical detection of assemblage E, Giardia duodenalis in scouring North Dakota calves
Article Snippet: .. Endogenous peroxidase activity was blocked by a 5 min incubation in 3% hydrogen peroxide after which slides were washed in Dako cytomation wash buffer [Tris buffer saline, with Tween 20, pH 7.6 (TBS)]. .. Following this step, the sections were permeabilized with proteinase K for 5 min, reacted with DAKO protein blocking solution (0.25% casein in phosphate buffered saline, pH 7.4) for 5 min and then incubated for 30 min with an anti-Giardia goat IgG polyclonal antibody diluted at 1:160 in DAKO cytomation antibody diluent (Tris–HCl with Tween 20).

Activity Assay:

Article Title: Differential CD4+ T-Lymphocyte Apoptosis and Bystander T-Cell Activation in Rhesus Macaques and Sooty Mangabeys during Acute Simian Immunodeficiency Virus Infection ▿
Article Snippet: .. Endogenous peroxidase activity was blocked by incubation in 3% H2 O2 , and antigen retrieval was accomplished by microwaving sections for 20 min in citrate buffer (Dako Corp., Carpinteria, CA). .. Tissue sections were treated for nonspecific protein binding (Protein Block; Dako) and then sequentially incubated for 30 min at room temperature with polyclonal rabbit anti-human cleaved caspase-3 (Cell Signaling Technology, Inc., Boston, MA) followed by biotinylated goat anti-rabbit IgG.

Article Title: p53 expression in colorectal carcinoma in relation to histopathological features in Ugandan patients
Article Snippet: .. Endogenous peroxidase activity of the tissue was blocked with 3% hydrogen peroxide for 10 min (Dako Denmark K4065) and sections were washed three times. .. Sections were incubated with monoclonal antibody p53 DO-7 (Dako Denmark) for 60 min followed by wash in PBS.

Article Title: STAT3 Mutation Is Associated with STAT3 Activation in CD30+ ALK− ALCL
Article Snippet: .. The endogenous peroxidase activity was blocked with Peroxidase-Blocking Solution (Dako/Agilent Tehnologies). .. The antibodies used were rabbit monoclonal antibodies from Cell Signaling (Leiden, the Netherlands): clone Tyr705 for pY-STAT3 (1:300), clone Thr202/Tyr204 for Phospho-p44/42MAPK (1:300) and Thr450 for Phospho-AKT (1:100).

Article Title: Mitotic activity index and CD25+ lymphocytes predict risk of stage progression in non-muscle invasive bladder cancer
Article Snippet: .. Endogenous peroxidase activity was inactivated by the incubation of tissue sections in the peroxidase-blocking reagent (DAKO, Glostrup, Denmark; S2001) for 10 min. Immunostaining of CD4+, CD8+, CD25+ T lymphocyte subsets, CD138+ plasma cells and proliferation marker Ki67 was performed using an Autostainer (DAKO, Glostrup, Denmark). .. The tissue sections were incubated with the monoclonal antibodies using the following dilutions: CD4 (Novocastra, Newcastle upon Tyne, UK; clone 1F6, 1:20); CD8 (DAKO, Glostrup, Denmark; clone C8/144B, 1:50); CD25 (Novocastra, Newcastle upon Tyne, UK; clone 4C9, 1:150); CD138 (Serotec, Kidlington, UK; clone B‑B4, 1:200) and Ki67 (DAKO, Glostrup, Denmark; clone MIB-1, 1:100).

Article Title: The HLA-A2 Restricted T Cell Epitope HCV Core35–44 Stabilizes HLA-E Expression and Inhibits Cytolysis Mediated by Natural Killer Cells
Article Snippet: .. In brief, endogenous peroxidase activity was blocked by 0.03% H2 O2 /NaNO3 (peroxidase blocking reagent, Dako, Carpinteria, CA). .. The sections were incubated with the HLA-E-specific antibody 3D12 in phosphate-buffered saline (PBS)/1% fetal calf serum (FCS) in a moist chamber at room temperature for 90 minutes.

Article Title: SARS spike protein induces phenotypic conversion of human B cells to macrophage-like cells
Article Snippet: .. Following blockage of the endogenous peroxidase activity and nonspecific antibody binding sites with 3% H2 O2 /0.2% NaN3 [15 min, room temperature (RT)] and 5% goat serum (10 min, RT), respectively, slides were incubated with monoclonal antibodies specific to human CD-68 (DAKO, Glostrup, Denmark) at RT for 90 min, before further treatment with LSAB2 (DAKO). .. Chromogenic development was processed with peroxidase-conjugated streptavidin and aminoethylcarbazole (Sigma, St. Louis, MO) solution.

Article Title: Low Expression of Single-stranded DNA Binding Protein 2 (SSBP2) Predicts Unfavourable Postoperative Outcomes in Patients With Clear Cell Renal Cell Carcinoma
Article Snippet: .. For antigen retrieval, the sections were heated in sodium citrate buffer (pH 6.0) in an autoclave at 100˚C for 20 min. Endogenous peroxidase activity was blocked with peroxidase blocking solution (S2023; Dako, Glostrup, Denmark). .. The TMA slides were incubated with primary antibodies at 4˚C overnight and then incubated with a labelled polymer (EnVision/HRP, K5007; Dako) for 30 min at room temperature.

Article Title: Molecular and immunohistochemical detection of assemblage E, Giardia duodenalis in scouring North Dakota calves
Article Snippet: .. Endogenous peroxidase activity was blocked by a 5 min incubation in 3% hydrogen peroxide after which slides were washed in Dako cytomation wash buffer [Tris buffer saline, with Tween 20, pH 7.6 (TBS)]. .. Following this step, the sections were permeabilized with proteinase K for 5 min, reacted with DAKO protein blocking solution (0.25% casein in phosphate buffered saline, pH 7.4) for 5 min and then incubated for 30 min with an anti-Giardia goat IgG polyclonal antibody diluted at 1:160 in DAKO cytomation antibody diluent (Tris–HCl with Tween 20).

Blocking Assay:

Article Title: The HLA-A2 Restricted T Cell Epitope HCV Core35–44 Stabilizes HLA-E Expression and Inhibits Cytolysis Mediated by Natural Killer Cells
Article Snippet: .. In brief, endogenous peroxidase activity was blocked by 0.03% H2 O2 /NaNO3 (peroxidase blocking reagent, Dako, Carpinteria, CA). .. The sections were incubated with the HLA-E-specific antibody 3D12 in phosphate-buffered saline (PBS)/1% fetal calf serum (FCS) in a moist chamber at room temperature for 90 minutes.

Article Title: Low Expression of Single-stranded DNA Binding Protein 2 (SSBP2) Predicts Unfavourable Postoperative Outcomes in Patients With Clear Cell Renal Cell Carcinoma
Article Snippet: .. For antigen retrieval, the sections were heated in sodium citrate buffer (pH 6.0) in an autoclave at 100˚C for 20 min. Endogenous peroxidase activity was blocked with peroxidase blocking solution (S2023; Dako, Glostrup, Denmark). .. The TMA slides were incubated with primary antibodies at 4˚C overnight and then incubated with a labelled polymer (EnVision/HRP, K5007; Dako) for 30 min at room temperature.

Marker:

Article Title: Mitotic activity index and CD25+ lymphocytes predict risk of stage progression in non-muscle invasive bladder cancer
Article Snippet: .. Endogenous peroxidase activity was inactivated by the incubation of tissue sections in the peroxidase-blocking reagent (DAKO, Glostrup, Denmark; S2001) for 10 min. Immunostaining of CD4+, CD8+, CD25+ T lymphocyte subsets, CD138+ plasma cells and proliferation marker Ki67 was performed using an Autostainer (DAKO, Glostrup, Denmark). .. The tissue sections were incubated with the monoclonal antibodies using the following dilutions: CD4 (Novocastra, Newcastle upon Tyne, UK; clone 1F6, 1:20); CD8 (DAKO, Glostrup, Denmark; clone C8/144B, 1:50); CD25 (Novocastra, Newcastle upon Tyne, UK; clone 4C9, 1:150); CD138 (Serotec, Kidlington, UK; clone B‑B4, 1:200) and Ki67 (DAKO, Glostrup, Denmark; clone MIB-1, 1:100).

Binding Assay:

Article Title: SARS spike protein induces phenotypic conversion of human B cells to macrophage-like cells
Article Snippet: .. Following blockage of the endogenous peroxidase activity and nonspecific antibody binding sites with 3% H2 O2 /0.2% NaN3 [15 min, room temperature (RT)] and 5% goat serum (10 min, RT), respectively, slides were incubated with monoclonal antibodies specific to human CD-68 (DAKO, Glostrup, Denmark) at RT for 90 min, before further treatment with LSAB2 (DAKO). .. Chromogenic development was processed with peroxidase-conjugated streptavidin and aminoethylcarbazole (Sigma, St. Louis, MO) solution.

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    Agilent technologies endogenous peroxidase activity
    Endogenous Peroxidase Activity, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 1237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endogenous peroxidase activity/product/Agilent technologies
    Average 94 stars, based on 1237 article reviews
    Price from $9.99 to $1999.99
    endogenous peroxidase activity - by Bioz Stars, 2020-11
    94/100 stars
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