end repair reaction buffer klenow fragment  (New England Biolabs)


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    Structured Review

    New England Biolabs end repair reaction buffer klenow fragment
    End Repair Reaction Buffer Klenow Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/end repair reaction buffer klenow fragment/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    end repair reaction buffer klenow fragment - by Bioz Stars, 2019-12
    86/100 stars

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    Related Articles

    RNA HS Assay:

    Article Title: Genome-wide identification of transcript start and end sites by Transcript Isoform Sequencing, TIF-Seq
    Article Snippet: T4 DNA ligase (NEB, 2000 U μL-1 , cat. no. M0202T) supplied with 10x T4 DNA Ligase Reaction Buffer BSA, Molecular Biology Grade (20 mg mL-1 , NEB, cat. no. B9000S) Plasmid-Safe ATP-Dependent DNase (10 U μL-1 , Epicentre, cat. no. E3101K) microTUBE AFA Fiber Pre-Slit Snap-Cap 6×16mm (Covaris, cat. no. 520045) Dynabeads M-280 streptavidin (Invitrogen, cat. no. 11205D) Bind and Wash buffer (B & W) (for a 2x concentration: 10 mM Tris-Cl pH 7.5, 1 mM EDTA pH 8.0 and 2 M NaCl in water) NEBNext End Repair Module (NEB, cat. no. E6050S) including NEBNext End Repair Enzyme Mix and 10x NEBNext End Repair Reaction Buffer Klenow Fragment (3′→5′ exo-) (NEB, 5 U μL-1 , cat. no. M0212S) supplied with 10x NEBuffer 2 (10x, NEB, cat. no. B7002S) dA tailing buffer (NEBuffer 2 (NEB, cat. no. B7002S) supplemented with 2 mM dATP) dATP 100 mM (BioLabs, cat. no. N0440S) dNTP mix 10 mM (BioLabs, cat no. N0447L) Quick Ligation Kit (NEB, cat. no. M2200S) including 2x Quick Ligation Buffer and Quick T4 DNA Ligase IVT spike-ins derived from B. subtilis (ATCC cat. no. 87482, 87483 and 87484). .. T4 DNA ligase (NEB, 2000 U μL-1 , cat. no. M0202T) supplied with 10x T4 DNA Ligase Reaction Buffer BSA, Molecular Biology Grade (20 mg mL-1 , NEB, cat. no. B9000S) Plasmid-Safe ATP-Dependent DNase (10 U μL-1 , Epicentre, cat. no. E3101K) microTUBE AFA Fiber Pre-Slit Snap-Cap 6×16mm (Covaris, cat. no. 520045) Dynabeads M-280 streptavidin (Invitrogen, cat. no. 11205D) Bind and Wash buffer (B & W) (for a 2x concentration: 10 mM Tris-Cl pH 7.5, 1 mM EDTA pH 8.0 and 2 M NaCl in water) NEBNext End Repair Module (NEB, cat. no. E6050S) including NEBNext End Repair Enzyme Mix and 10x NEBNext End Repair Reaction Buffer Klenow Fragment (3′→5′ exo-) (NEB, 5 U μL-1 , cat. no. M0212S) supplied with 10x NEBuffer 2 (10x, NEB, cat. no. B7002S) dA tailing buffer (NEBuffer 2 (NEB, cat. no. B7002S) supplemented with 2 mM dATP) dATP 100 mM (BioLabs, cat. no. N0440S) dNTP mix 10 mM (BioLabs, cat no. N0447L) Quick Ligation Kit (NEB, cat. no. M2200S) including 2x Quick Ligation Buffer and Quick T4 DNA Ligase IVT spike-ins derived from B. subtilis (ATCC cat. no. 87482, 87483 and 87484).

    Ligation:

    Article Title: Genome-wide identification of transcript start and end sites by Transcript Isoform Sequencing, TIF-Seq
    Article Snippet: Note that the new NotI-HF (20 U μL-1 , NEB, cat. no. R3189S) can also be used. .. T4 DNA ligase (NEB, 2000 U μL-1 , cat. no. M0202T) supplied with 10x T4 DNA Ligase Reaction Buffer BSA, Molecular Biology Grade (20 mg mL-1 , NEB, cat. no. B9000S) Plasmid-Safe ATP-Dependent DNase (10 U μL-1 , Epicentre, cat. no. E3101K) microTUBE AFA Fiber Pre-Slit Snap-Cap 6×16mm (Covaris, cat. no. 520045) Dynabeads M-280 streptavidin (Invitrogen, cat. no. 11205D) Bind and Wash buffer (B & W) (for a 2x concentration: 10 mM Tris-Cl pH 7.5, 1 mM EDTA pH 8.0 and 2 M NaCl in water) NEBNext End Repair Module (NEB, cat. no. E6050S) including NEBNext End Repair Enzyme Mix and 10x NEBNext End Repair Reaction Buffer Klenow Fragment (3′→5′ exo-) (NEB, 5 U μL-1 , cat. no. M0212S) supplied with 10x NEBuffer 2 (10x, NEB, cat. no. B7002S) dA tailing buffer (NEBuffer 2 (NEB, cat. no. B7002S) supplemented with 2 mM dATP) dATP 100 mM (BioLabs, cat. no. N0440S) dNTP mix 10 mM (BioLabs, cat no. N0447L) Quick Ligation Kit (NEB, cat. no. M2200S) including 2x Quick Ligation Buffer and Quick T4 DNA Ligase IVT spike-ins derived from B. subtilis (ATCC cat. no. 87482, 87483 and 87484). .. T3 RNA polymerase (Promega, 10 U μL-1 , cat. no. P2083) including DTT (100 mM, cat. no. P117B) and Transcription Optimized 5x buffer (cat. no. P118B) Vaccinia Capping System (NEB, 10 U μL-1 , cat. no. M2080S) supplied with 10x Capping Buffer, S-adenosylmethionine (SAM, 32 mM) and GTP (10 mM) Agilent High Sensitivity DNA Kit (Agilent, cat. no. 5067-4626) CRITICAL: It should be taken out of the fridge in advance to reach room temperature (20-25°C) before its use.

    Isolation:

    Article Title: Genome-wide quantification of 5' phosphorylated mRNA degradation intermediates for analysis of ribosome dynamics
    Article Snippet: Sodium acetate buffer solution (3 M, pH 5.2, Sigma, cat. no. S7899) Linear Acrylamide (5 mg ml−1 , Life technologies, cat. no. AM9520) T4 RNA Ligase 1 (ssRNA Ligase) (10 U µL−1 , NEB, cat. no. M0204S) supplied with 10× T4 RNA ligase reaction buffer and Adenosine-5'-Triphosphate (ATP, 10 mM) Dimethyl sulfoxide (DMSO) (BioLabs, cat. no. B0515A) HighPrep PCR (Magbio cat. no. AC-60050) Dynabeads® Oligo(dT)25 mRNA isolation beads (Life technologies, cat. no. 61002) Binding buffer for Oligo(dT)25 (20 mM Tris-HCl, pH 7.5, 1 M LiCl, 2 mM EDTA). .. SuperScript II Reverse Transcriptase (200 U µL−1 , Life technologies, cat. no. 18064-022) supplied with 0.1 M DTT and 5× first-strand buffer Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB, cat. no. M0531S) T4 DNA ligase (NEB, 2000 U µL−1 , cat. no. M0202T) supplied with 10× T4 DNA Ligase Reaction Buffer Dynabeads M-280 streptavidin (Life technologies, cat. no. 11205D) Bind and Wash buffer (B & W) (for a 2× concentration: 10 mM Tris-Cl pH 7.5, 1 mM EDTA pH 8.0 and 2 M NaCl in water) NEBNext End Repair Module (NEB, cat. no. E6050S) including NEBNext End Repair Enzyme Mix and 10× NEBNext End Repair Reaction Buffer Klenow Fragment (3'→5' exo-) (NEB, 5 U µL−1 , cat. no. M0212S) supplied with 10× NEBuffer 2 (10×, NEB, cat. no. B7002S) dA tailing buffer (NEBuffer 2 (NEB, cat. no. B7002S) supplemented with 2 mM dATP) dATP 100 mM (BioLabs, cat. no. N0440S) dNTP mix 10 mM (BioLabs, cat no. N0447L) Agilent High Sensitivity DNA Kit (Agilent, cat. no. 5067-4626) CRITICAL: It should be taken out of the fridge in advance to reach room temperature (20–25°C) before its use.

    Purification:

    Article Title: Genome-wide identification of transcript start and end sites by Transcript Isoform Sequencing, TIF-Seq
    Article Snippet: Dimethyl sulfoxide (DMSO) (BioLabs, cat. no. B0515A) Agencourt AMPure XP - PCR Purification (Beckman Coulter, cat. no. ) SuperScript III Reverse Transcriptase (200 U μL-1 , Invitrogen, cat. no. 18080-044) supplied with 0.1 M DTT and 5x first-strand buffer Trehalose 1.57 M dissolved in water from solid (Sigma, cat. no. T3663) RNase H (10 U μL-1 , Epicenter, cat. no. R0601K) RNase Cocktail (0.5U μL-1 RNase A and 20 U μL-1 RNase T1, Invitrogen, cat. no. AM2286) Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB, cat. no. M0531S) Agarose (Sigma, cat. no. A9539) TBE (for 1 L 10x solution mix 108 g Tris base (Trizma, Sigma, cat. no. T1503), 55 g boric acid (Merck, cat. no.100162), 960 ml water and 40 ml 0.5 M EDTA, pH 8.0. .. T4 DNA ligase (NEB, 2000 U μL-1 , cat. no. M0202T) supplied with 10x T4 DNA Ligase Reaction Buffer BSA, Molecular Biology Grade (20 mg mL-1 , NEB, cat. no. B9000S) Plasmid-Safe ATP-Dependent DNase (10 U μL-1 , Epicentre, cat. no. E3101K) microTUBE AFA Fiber Pre-Slit Snap-Cap 6×16mm (Covaris, cat. no. 520045) Dynabeads M-280 streptavidin (Invitrogen, cat. no. 11205D) Bind and Wash buffer (B & W) (for a 2x concentration: 10 mM Tris-Cl pH 7.5, 1 mM EDTA pH 8.0 and 2 M NaCl in water) NEBNext End Repair Module (NEB, cat. no. E6050S) including NEBNext End Repair Enzyme Mix and 10x NEBNext End Repair Reaction Buffer Klenow Fragment (3′→5′ exo-) (NEB, 5 U μL-1 , cat. no. M0212S) supplied with 10x NEBuffer 2 (10x, NEB, cat. no. B7002S) dA tailing buffer (NEBuffer 2 (NEB, cat. no. B7002S) supplemented with 2 mM dATP) dATP 100 mM (BioLabs, cat. no. N0440S) dNTP mix 10 mM (BioLabs, cat no. N0447L) Quick Ligation Kit (NEB, cat. no. M2200S) including 2x Quick Ligation Buffer and Quick T4 DNA Ligase IVT spike-ins derived from B. subtilis (ATCC cat. no. 87482, 87483 and 87484).

    Plasmid Preparation:

    Article Title: Genome-wide identification of transcript start and end sites by Transcript Isoform Sequencing, TIF-Seq
    Article Snippet: Note that the new NotI-HF (20 U μL-1 , NEB, cat. no. R3189S) can also be used. .. T4 DNA ligase (NEB, 2000 U μL-1 , cat. no. M0202T) supplied with 10x T4 DNA Ligase Reaction Buffer BSA, Molecular Biology Grade (20 mg mL-1 , NEB, cat. no. B9000S) Plasmid-Safe ATP-Dependent DNase (10 U μL-1 , Epicentre, cat. no. E3101K) microTUBE AFA Fiber Pre-Slit Snap-Cap 6×16mm (Covaris, cat. no. 520045) Dynabeads M-280 streptavidin (Invitrogen, cat. no. 11205D) Bind and Wash buffer (B & W) (for a 2x concentration: 10 mM Tris-Cl pH 7.5, 1 mM EDTA pH 8.0 and 2 M NaCl in water) NEBNext End Repair Module (NEB, cat. no. E6050S) including NEBNext End Repair Enzyme Mix and 10x NEBNext End Repair Reaction Buffer Klenow Fragment (3′→5′ exo-) (NEB, 5 U μL-1 , cat. no. M0212S) supplied with 10x NEBuffer 2 (10x, NEB, cat. no. B7002S) dA tailing buffer (NEBuffer 2 (NEB, cat. no. B7002S) supplemented with 2 mM dATP) dATP 100 mM (BioLabs, cat. no. N0440S) dNTP mix 10 mM (BioLabs, cat no. N0447L) Quick Ligation Kit (NEB, cat. no. M2200S) including 2x Quick Ligation Buffer and Quick T4 DNA Ligase IVT spike-ins derived from B. subtilis (ATCC cat. no. 87482, 87483 and 87484). .. T3 RNA polymerase (Promega, 10 U μL-1 , cat. no. P2083) including DTT (100 mM, cat. no. P117B) and Transcription Optimized 5x buffer (cat. no. P118B) Vaccinia Capping System (NEB, 10 U μL-1 , cat. no. M2080S) supplied with 10x Capping Buffer, S-adenosylmethionine (SAM, 32 mM) and GTP (10 mM) Agilent High Sensitivity DNA Kit (Agilent, cat. no. 5067-4626) CRITICAL: It should be taken out of the fridge in advance to reach room temperature (20-25°C) before its use.

    Concentration Assay:

    Article Title: Genome-wide quantification of 5' phosphorylated mRNA degradation intermediates for analysis of ribosome dynamics
    Article Snippet: Wash buffer B for Oligo(dT)25 (10 mM Tris-HCl, pH 7.5, 0.15 M LiCl, 1 mM EDTA). .. SuperScript II Reverse Transcriptase (200 U µL−1 , Life technologies, cat. no. 18064-022) supplied with 0.1 M DTT and 5× first-strand buffer Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB, cat. no. M0531S) T4 DNA ligase (NEB, 2000 U µL−1 , cat. no. M0202T) supplied with 10× T4 DNA Ligase Reaction Buffer Dynabeads M-280 streptavidin (Life technologies, cat. no. 11205D) Bind and Wash buffer (B & W) (for a 2× concentration: 10 mM Tris-Cl pH 7.5, 1 mM EDTA pH 8.0 and 2 M NaCl in water) NEBNext End Repair Module (NEB, cat. no. E6050S) including NEBNext End Repair Enzyme Mix and 10× NEBNext End Repair Reaction Buffer Klenow Fragment (3'→5' exo-) (NEB, 5 U µL−1 , cat. no. M0212S) supplied with 10× NEBuffer 2 (10×, NEB, cat. no. B7002S) dA tailing buffer (NEBuffer 2 (NEB, cat. no. B7002S) supplemented with 2 mM dATP) dATP 100 mM (BioLabs, cat. no. N0440S) dNTP mix 10 mM (BioLabs, cat no. N0447L) Agilent High Sensitivity DNA Kit (Agilent, cat. no. 5067-4626) CRITICAL: It should be taken out of the fridge in advance to reach room temperature (20–25°C) before its use. .. Agilent RNA 6000 Nano Kit (Agilent, cat. no. 5067-1511) CRITICAL: It should be taken out of the fridge in advance to reach room temperature (20–25°C) before its use. dsDNA HS Assay kit for Qubit 2.0 (Life technologies, cat. no. ) RNA HS Assay kit for Qubit 2.0 (Life technologies, cat. no. )

    Article Title: Genome-wide identification of transcript start and end sites by Transcript Isoform Sequencing, TIF-Seq
    Article Snippet: Note that the new NotI-HF (20 U μL-1 , NEB, cat. no. R3189S) can also be used. .. T4 DNA ligase (NEB, 2000 U μL-1 , cat. no. M0202T) supplied with 10x T4 DNA Ligase Reaction Buffer BSA, Molecular Biology Grade (20 mg mL-1 , NEB, cat. no. B9000S) Plasmid-Safe ATP-Dependent DNase (10 U μL-1 , Epicentre, cat. no. E3101K) microTUBE AFA Fiber Pre-Slit Snap-Cap 6×16mm (Covaris, cat. no. 520045) Dynabeads M-280 streptavidin (Invitrogen, cat. no. 11205D) Bind and Wash buffer (B & W) (for a 2x concentration: 10 mM Tris-Cl pH 7.5, 1 mM EDTA pH 8.0 and 2 M NaCl in water) NEBNext End Repair Module (NEB, cat. no. E6050S) including NEBNext End Repair Enzyme Mix and 10x NEBNext End Repair Reaction Buffer Klenow Fragment (3′→5′ exo-) (NEB, 5 U μL-1 , cat. no. M0212S) supplied with 10x NEBuffer 2 (10x, NEB, cat. no. B7002S) dA tailing buffer (NEBuffer 2 (NEB, cat. no. B7002S) supplemented with 2 mM dATP) dATP 100 mM (BioLabs, cat. no. N0440S) dNTP mix 10 mM (BioLabs, cat no. N0447L) Quick Ligation Kit (NEB, cat. no. M2200S) including 2x Quick Ligation Buffer and Quick T4 DNA Ligase IVT spike-ins derived from B. subtilis (ATCC cat. no. 87482, 87483 and 87484). .. T3 RNA polymerase (Promega, 10 U μL-1 , cat. no. P2083) including DTT (100 mM, cat. no. P117B) and Transcription Optimized 5x buffer (cat. no. P118B) Vaccinia Capping System (NEB, 10 U μL-1 , cat. no. M2080S) supplied with 10x Capping Buffer, S-adenosylmethionine (SAM, 32 mM) and GTP (10 mM) Agilent High Sensitivity DNA Kit (Agilent, cat. no. 5067-4626) CRITICAL: It should be taken out of the fridge in advance to reach room temperature (20-25°C) before its use.

    Hood:

    Article Title: Genome-wide quantification of 5' phosphorylated mRNA degradation intermediates for analysis of ribosome dynamics
    Article Snippet: SuperScript II Reverse Transcriptase (200 U µL−1 , Life technologies, cat. no. 18064-022) supplied with 0.1 M DTT and 5× first-strand buffer Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB, cat. no. M0531S) T4 DNA ligase (NEB, 2000 U µL−1 , cat. no. M0202T) supplied with 10× T4 DNA Ligase Reaction Buffer Dynabeads M-280 streptavidin (Life technologies, cat. no. 11205D) Bind and Wash buffer (B & W) (for a 2× concentration: 10 mM Tris-Cl pH 7.5, 1 mM EDTA pH 8.0 and 2 M NaCl in water) NEBNext End Repair Module (NEB, cat. no. E6050S) including NEBNext End Repair Enzyme Mix and 10× NEBNext End Repair Reaction Buffer Klenow Fragment (3'→5' exo-) (NEB, 5 U µL−1 , cat. no. M0212S) supplied with 10× NEBuffer 2 (10×, NEB, cat. no. B7002S) dA tailing buffer (NEBuffer 2 (NEB, cat. no. B7002S) supplemented with 2 mM dATP) dATP 100 mM (BioLabs, cat. no. N0440S) dNTP mix 10 mM (BioLabs, cat no. N0447L) Agilent High Sensitivity DNA Kit (Agilent, cat. no. 5067-4626) CRITICAL: It should be taken out of the fridge in advance to reach room temperature (20–25°C) before its use. .. SuperScript II Reverse Transcriptase (200 U µL−1 , Life technologies, cat. no. 18064-022) supplied with 0.1 M DTT and 5× first-strand buffer Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB, cat. no. M0531S) T4 DNA ligase (NEB, 2000 U µL−1 , cat. no. M0202T) supplied with 10× T4 DNA Ligase Reaction Buffer Dynabeads M-280 streptavidin (Life technologies, cat. no. 11205D) Bind and Wash buffer (B & W) (for a 2× concentration: 10 mM Tris-Cl pH 7.5, 1 mM EDTA pH 8.0 and 2 M NaCl in water) NEBNext End Repair Module (NEB, cat. no. E6050S) including NEBNext End Repair Enzyme Mix and 10× NEBNext End Repair Reaction Buffer Klenow Fragment (3'→5' exo-) (NEB, 5 U µL−1 , cat. no. M0212S) supplied with 10× NEBuffer 2 (10×, NEB, cat. no. B7002S) dA tailing buffer (NEBuffer 2 (NEB, cat. no. B7002S) supplemented with 2 mM dATP) dATP 100 mM (BioLabs, cat. no. N0440S) dNTP mix 10 mM (BioLabs, cat no. N0447L) Agilent High Sensitivity DNA Kit (Agilent, cat. no. 5067-4626) CRITICAL: It should be taken out of the fridge in advance to reach room temperature (20–25°C) before its use.

    Polymerase Chain Reaction:

    Article Title: Genome-wide quantification of 5' phosphorylated mRNA degradation intermediates for analysis of ribosome dynamics
    Article Snippet: Wash buffer B for Oligo(dT)25 (10 mM Tris-HCl, pH 7.5, 0.15 M LiCl, 1 mM EDTA). .. SuperScript II Reverse Transcriptase (200 U µL−1 , Life technologies, cat. no. 18064-022) supplied with 0.1 M DTT and 5× first-strand buffer Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB, cat. no. M0531S) T4 DNA ligase (NEB, 2000 U µL−1 , cat. no. M0202T) supplied with 10× T4 DNA Ligase Reaction Buffer Dynabeads M-280 streptavidin (Life technologies, cat. no. 11205D) Bind and Wash buffer (B & W) (for a 2× concentration: 10 mM Tris-Cl pH 7.5, 1 mM EDTA pH 8.0 and 2 M NaCl in water) NEBNext End Repair Module (NEB, cat. no. E6050S) including NEBNext End Repair Enzyme Mix and 10× NEBNext End Repair Reaction Buffer Klenow Fragment (3'→5' exo-) (NEB, 5 U µL−1 , cat. no. M0212S) supplied with 10× NEBuffer 2 (10×, NEB, cat. no. B7002S) dA tailing buffer (NEBuffer 2 (NEB, cat. no. B7002S) supplemented with 2 mM dATP) dATP 100 mM (BioLabs, cat. no. N0440S) dNTP mix 10 mM (BioLabs, cat no. N0447L) Agilent High Sensitivity DNA Kit (Agilent, cat. no. 5067-4626) CRITICAL: It should be taken out of the fridge in advance to reach room temperature (20–25°C) before its use. .. Agilent RNA 6000 Nano Kit (Agilent, cat. no. 5067-1511) CRITICAL: It should be taken out of the fridge in advance to reach room temperature (20–25°C) before its use. dsDNA HS Assay kit for Qubit 2.0 (Life technologies, cat. no. ) RNA HS Assay kit for Qubit 2.0 (Life technologies, cat. no. )

    Article Title: Genome-wide identification of transcript start and end sites by Transcript Isoform Sequencing, TIF-Seq
    Article Snippet: Dimethyl sulfoxide (DMSO) (BioLabs, cat. no. B0515A) Agencourt AMPure XP - PCR Purification (Beckman Coulter, cat. no. ) SuperScript III Reverse Transcriptase (200 U μL-1 , Invitrogen, cat. no. 18080-044) supplied with 0.1 M DTT and 5x first-strand buffer Trehalose 1.57 M dissolved in water from solid (Sigma, cat. no. T3663) RNase H (10 U μL-1 , Epicenter, cat. no. R0601K) RNase Cocktail (0.5U μL-1 RNase A and 20 U μL-1 RNase T1, Invitrogen, cat. no. AM2286) Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB, cat. no. M0531S) Agarose (Sigma, cat. no. A9539) TBE (for 1 L 10x solution mix 108 g Tris base (Trizma, Sigma, cat. no. T1503), 55 g boric acid (Merck, cat. no.100162), 960 ml water and 40 ml 0.5 M EDTA, pH 8.0. .. T4 DNA ligase (NEB, 2000 U μL-1 , cat. no. M0202T) supplied with 10x T4 DNA Ligase Reaction Buffer BSA, Molecular Biology Grade (20 mg mL-1 , NEB, cat. no. B9000S) Plasmid-Safe ATP-Dependent DNase (10 U μL-1 , Epicentre, cat. no. E3101K) microTUBE AFA Fiber Pre-Slit Snap-Cap 6×16mm (Covaris, cat. no. 520045) Dynabeads M-280 streptavidin (Invitrogen, cat. no. 11205D) Bind and Wash buffer (B & W) (for a 2x concentration: 10 mM Tris-Cl pH 7.5, 1 mM EDTA pH 8.0 and 2 M NaCl in water) NEBNext End Repair Module (NEB, cat. no. E6050S) including NEBNext End Repair Enzyme Mix and 10x NEBNext End Repair Reaction Buffer Klenow Fragment (3′→5′ exo-) (NEB, 5 U μL-1 , cat. no. M0212S) supplied with 10x NEBuffer 2 (10x, NEB, cat. no. B7002S) dA tailing buffer (NEBuffer 2 (NEB, cat. no. B7002S) supplemented with 2 mM dATP) dATP 100 mM (BioLabs, cat. no. N0440S) dNTP mix 10 mM (BioLabs, cat no. N0447L) Quick Ligation Kit (NEB, cat. no. M2200S) including 2x Quick Ligation Buffer and Quick T4 DNA Ligase IVT spike-ins derived from B. subtilis (ATCC cat. no. 87482, 87483 and 87484).

    Gel Extraction:

    Article Title: Genome-wide identification of transcript start and end sites by Transcript Isoform Sequencing, TIF-Seq
    Article Snippet: Non-migrating DNA gel loading buffer dye (15% (wt/vol) Ficoll 400, blue dextran, 5 mM EDTA) QIAquick Gel Extraction Kit (Qiagen, cat. no. 28704) supplied with Elution Buffer (EB) (10 mM Tris-Cl, pH 8.5, Qiagen, cat. no. 19086) NotI restriction enzyme (10 U μL-1 , NEB, cat. no. R0189S) supplied with 10x NEBuffer 3(10x, NEB, cat. no. B7003S). .. T4 DNA ligase (NEB, 2000 U μL-1 , cat. no. M0202T) supplied with 10x T4 DNA Ligase Reaction Buffer BSA, Molecular Biology Grade (20 mg mL-1 , NEB, cat. no. B9000S) Plasmid-Safe ATP-Dependent DNase (10 U μL-1 , Epicentre, cat. no. E3101K) microTUBE AFA Fiber Pre-Slit Snap-Cap 6×16mm (Covaris, cat. no. 520045) Dynabeads M-280 streptavidin (Invitrogen, cat. no. 11205D) Bind and Wash buffer (B & W) (for a 2x concentration: 10 mM Tris-Cl pH 7.5, 1 mM EDTA pH 8.0 and 2 M NaCl in water) NEBNext End Repair Module (NEB, cat. no. E6050S) including NEBNext End Repair Enzyme Mix and 10x NEBNext End Repair Reaction Buffer Klenow Fragment (3′→5′ exo-) (NEB, 5 U μL-1 , cat. no. M0212S) supplied with 10x NEBuffer 2 (10x, NEB, cat. no. B7002S) dA tailing buffer (NEBuffer 2 (NEB, cat. no. B7002S) supplemented with 2 mM dATP) dATP 100 mM (BioLabs, cat. no. N0440S) dNTP mix 10 mM (BioLabs, cat no. N0447L) Quick Ligation Kit (NEB, cat. no. M2200S) including 2x Quick Ligation Buffer and Quick T4 DNA Ligase IVT spike-ins derived from B. subtilis (ATCC cat. no. 87482, 87483 and 87484).

    Binding Assay:

    Article Title: Genome-wide quantification of 5' phosphorylated mRNA degradation intermediates for analysis of ribosome dynamics
    Article Snippet: Sodium acetate buffer solution (3 M, pH 5.2, Sigma, cat. no. S7899) Linear Acrylamide (5 mg ml−1 , Life technologies, cat. no. AM9520) T4 RNA Ligase 1 (ssRNA Ligase) (10 U µL−1 , NEB, cat. no. M0204S) supplied with 10× T4 RNA ligase reaction buffer and Adenosine-5'-Triphosphate (ATP, 10 mM) Dimethyl sulfoxide (DMSO) (BioLabs, cat. no. B0515A) HighPrep PCR (Magbio cat. no. AC-60050) Dynabeads® Oligo(dT)25 mRNA isolation beads (Life technologies, cat. no. 61002) Binding buffer for Oligo(dT)25 (20 mM Tris-HCl, pH 7.5, 1 M LiCl, 2 mM EDTA). .. SuperScript II Reverse Transcriptase (200 U µL−1 , Life technologies, cat. no. 18064-022) supplied with 0.1 M DTT and 5× first-strand buffer Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB, cat. no. M0531S) T4 DNA ligase (NEB, 2000 U µL−1 , cat. no. M0202T) supplied with 10× T4 DNA Ligase Reaction Buffer Dynabeads M-280 streptavidin (Life technologies, cat. no. 11205D) Bind and Wash buffer (B & W) (for a 2× concentration: 10 mM Tris-Cl pH 7.5, 1 mM EDTA pH 8.0 and 2 M NaCl in water) NEBNext End Repair Module (NEB, cat. no. E6050S) including NEBNext End Repair Enzyme Mix and 10× NEBNext End Repair Reaction Buffer Klenow Fragment (3'→5' exo-) (NEB, 5 U µL−1 , cat. no. M0212S) supplied with 10× NEBuffer 2 (10×, NEB, cat. no. B7002S) dA tailing buffer (NEBuffer 2 (NEB, cat. no. B7002S) supplemented with 2 mM dATP) dATP 100 mM (BioLabs, cat. no. N0440S) dNTP mix 10 mM (BioLabs, cat no. N0447L) Agilent High Sensitivity DNA Kit (Agilent, cat. no. 5067-4626) CRITICAL: It should be taken out of the fridge in advance to reach room temperature (20–25°C) before its use.

    Derivative Assay:

    Article Title: Genome-wide identification of transcript start and end sites by Transcript Isoform Sequencing, TIF-Seq
    Article Snippet: Note that the new NotI-HF (20 U μL-1 , NEB, cat. no. R3189S) can also be used. .. T4 DNA ligase (NEB, 2000 U μL-1 , cat. no. M0202T) supplied with 10x T4 DNA Ligase Reaction Buffer BSA, Molecular Biology Grade (20 mg mL-1 , NEB, cat. no. B9000S) Plasmid-Safe ATP-Dependent DNase (10 U μL-1 , Epicentre, cat. no. E3101K) microTUBE AFA Fiber Pre-Slit Snap-Cap 6×16mm (Covaris, cat. no. 520045) Dynabeads M-280 streptavidin (Invitrogen, cat. no. 11205D) Bind and Wash buffer (B & W) (for a 2x concentration: 10 mM Tris-Cl pH 7.5, 1 mM EDTA pH 8.0 and 2 M NaCl in water) NEBNext End Repair Module (NEB, cat. no. E6050S) including NEBNext End Repair Enzyme Mix and 10x NEBNext End Repair Reaction Buffer Klenow Fragment (3′→5′ exo-) (NEB, 5 U μL-1 , cat. no. M0212S) supplied with 10x NEBuffer 2 (10x, NEB, cat. no. B7002S) dA tailing buffer (NEBuffer 2 (NEB, cat. no. B7002S) supplemented with 2 mM dATP) dATP 100 mM (BioLabs, cat. no. N0440S) dNTP mix 10 mM (BioLabs, cat no. N0447L) Quick Ligation Kit (NEB, cat. no. M2200S) including 2x Quick Ligation Buffer and Quick T4 DNA Ligase IVT spike-ins derived from B. subtilis (ATCC cat. no. 87482, 87483 and 87484). .. T3 RNA polymerase (Promega, 10 U μL-1 , cat. no. P2083) including DTT (100 mM, cat. no. P117B) and Transcription Optimized 5x buffer (cat. no. P118B) Vaccinia Capping System (NEB, 10 U μL-1 , cat. no. M2080S) supplied with 10x Capping Buffer, S-adenosylmethionine (SAM, 32 mM) and GTP (10 mM) Agilent High Sensitivity DNA Kit (Agilent, cat. no. 5067-4626) CRITICAL: It should be taken out of the fridge in advance to reach room temperature (20-25°C) before its use.

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    New England Biolabs end repair reaction buffer klenow fragment
    End Repair Reaction Buffer Klenow Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/end repair reaction buffer klenow fragment/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    end repair reaction buffer klenow fragment - by Bioz Stars, 2019-12
    86/100 stars
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