end repair reaction buffer klenow fragment  (New England Biolabs)


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  • 99
    Name:
    Klenow Fragment 3 5 exo
    Description:
    Klenow Fragment 3 5 exo 1 000 units
    Catalog Number:
    m0212l
    Price:
    248
    Size:
    1 000 units
    Category:
    DNA Polymerases
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    Structured Review

    New England Biolabs end repair reaction buffer klenow fragment
    Klenow Fragment 3 5 exo
    Klenow Fragment 3 5 exo 1 000 units
    https://www.bioz.com/result/end repair reaction buffer klenow fragment/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    end repair reaction buffer klenow fragment - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: An evolved xylose transporter from Zymomonas mobilis enhances sugar transport in Escherichia coli
    Article Snippet: .. To facilitate the re-ligation (see below), the purified DNA was blunt-ended with 3.2 U Klenow fragment in 10× NEBuffer 2 (New England Biolabs) and dNTPs (final concentration 33 μM each nucleotide) for 15 min at 25°C. .. Then EDTA was added to a final concentration of 10 mM, and the tube was incubated at 75°C for 20 min (Figure ).

    Purification:

    Article Title: An evolved xylose transporter from Zymomonas mobilis enhances sugar transport in Escherichia coli
    Article Snippet: .. To facilitate the re-ligation (see below), the purified DNA was blunt-ended with 3.2 U Klenow fragment in 10× NEBuffer 2 (New England Biolabs) and dNTPs (final concentration 33 μM each nucleotide) for 15 min at 25°C. .. Then EDTA was added to a final concentration of 10 mM, and the tube was incubated at 75°C for 20 min (Figure ).

    Article Title: A library-based method to rapidly analyse chromatin accessibility at multiple genomic regions
    Article Snippet: .. To allow for ligation with the blunt-end of the double-stranded adaptors, 15 µl of purified MNase digested DNA fragments were blunt-ended in a final volume of 20 µl by Klenow fragment of Escherichia coli DNA polymerase (New England Biolabs, CA, USA) and phosphorylated by T4 polynucleotide kinase (New England Biolabs) in a final volume of 30 µl. .. Adaptor ligation The blunt-ended and phosphorylated DNA fragments were ligated to the double-stranded adaptors A and B as described in ( ) with the following modifications.

    other:

    Article Title: Protein Displacement by Herpes Helicase-Primase and the Key Role of UL42 During Helicase-Coupled DNA Synthesis by the Herpes Polymerase
    Article Snippet: Both polymerases could replace UL30-UL42, with Klenow Fragment generating products ~1 kB long.

    Article Title: Protein Displacement by Herpes Helicase-Primase and the Key Role of UL42 During Helicase-Coupled DNA Synthesis by the Herpes Polymerase
    Article Snippet: The absence of any products longer than ~100 nucleotides in assays containing UL30 is not due to either the lower processivity of UL30 relative to UL30-UL42 since UL30 and Klenow Fragment have similar processivity ( ) and Klenow Fragment generates long products.

    Article Title: Protein Displacement by Herpes Helicase-Primase and the Key Role of UL42 During Helicase-Coupled DNA Synthesis by the Herpes Polymerase
    Article Snippet: Even without ICP8, we found that non-cognate polymerases such as Klenow Fragment and T4 DNA polymerase allow the herpes helicase to more efficiently unwind DNA and allow the synthesis of long products.

    DNA Synthesis:

    Article Title: 5?CAG and 5?CTG Repeats Create Differential Impediment to the Progression of a Minimal Reconstituted T4 Replisome Depending on the Concentration of dNTPs
    Article Snippet: .. However, contrary to the gp43-gp41 replication couple, DNA synthesis performed by Klenow fragment was not stimulated by gp41 ( , compare lanes 2, 3, 5, and 6). .. Under both conditions (with or without gp41), DNA synthesis was highly distributive as indicated by the numerous pause sites that were clearly visible along the template strand.

    Ligation:

    Article Title: A library-based method to rapidly analyse chromatin accessibility at multiple genomic regions
    Article Snippet: .. To allow for ligation with the blunt-end of the double-stranded adaptors, 15 µl of purified MNase digested DNA fragments were blunt-ended in a final volume of 20 µl by Klenow fragment of Escherichia coli DNA polymerase (New England Biolabs, CA, USA) and phosphorylated by T4 polynucleotide kinase (New England Biolabs) in a final volume of 30 µl. .. Adaptor ligation The blunt-ended and phosphorylated DNA fragments were ligated to the double-stranded adaptors A and B as described in ( ) with the following modifications.

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  • 99
    New England Biolabs 10x nebnext end repair reaction buffer klenow fragment
    10x Nebnext End Repair Reaction Buffer Klenow Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x nebnext end repair reaction buffer klenow fragment/product/New England Biolabs
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    10x nebnext end repair reaction buffer klenow fragment - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs end repair reaction buffer klenow fragment
    End Repair Reaction Buffer Klenow Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/end repair reaction buffer klenow fragment/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    end repair reaction buffer klenow fragment - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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