end repair enzyme mix  (New England Biolabs)


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  • 99
    Name:
    NEBNext End Repair Reaction Buffer
    Description:
    NEBNext End Repair Reaction Buffer 2 0 ml
    Catalog Number:
    b6052s
    Price:
    42
    Size:
    2 0 ml
    Category:
    Buffers
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    Structured Review

    New England Biolabs end repair enzyme mix
    NEBNext End Repair Reaction Buffer
    NEBNext End Repair Reaction Buffer 2 0 ml
    https://www.bioz.com/result/end repair enzyme mix/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    end repair enzyme mix - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Mapping the micro-proteome of the nuclear lamina and lamin associated domains
    Article Snippet: .. DamID-seq Library Preparation and Sequencing In order to ensure sequencing of all DamID fragments, post-DamID amplified material was randomized by performing an end repair reaction, followed by ligation and sonication. .. Specifically, 0.5-5 μg of column purified DamID material (from above) was end-repaired using the NEBNext End Repair Module (NEB E6050S) following manufacturer’s recommendations.

    Ligation:

    Article Title: Genome-Wide Identification of Circular RNAs in Arabidopsis thaliana
    Article Snippet: .. Third, the short fragments were further resolved with NEBNext End Repair Reaction Buffer and NEBNext Pre Enzyme Mix for end repair and poly (A) ligation. ..

    Article Title: In vivo CRISPR editing with no detectable genome-wide off-target mutations
    Article Snippet: .. Pooled samples were end-repaired and A-tailed using an end prep enzyme mix and reaction buffer from NEBNext Ultra II DNA Library Prep Kit for Illumina, and ligated to Illumina TruSeq adapters using a ligation master mix and ligation enhancer from the same kit. .. Library-prepped samples were then purified with magnetic beads made as previously described , size-selected using PEG/NaCl SPRI solution (KAPA Biosystems), quantified using droplet digital PCR (BioRad), and loaded onto an Illumina MiSeq for deep sequencing.

    Article Title: Enabling high-accuracy long-read amplicon sequences using unique molecular identifiers with Nanopore or PacBio sequencing
    Article Snippet: .. End-repair and UMI adaptor ligation An end-repair reaction was prepared containing 7 µL NEBNext End Repair Reaction buffer, 3 µL NEBNext End Prep Enzyme mix (New England Biolabs, USA), 2.5 µg fragmented DNA and adjusted to 50 µL with nuclease free water (Qiagen, Netherlands). .. The DNA was purified using CleanPCR (CleanNA, Netherlands) and its concentration and quality was assessed as described above.

    Article Title: Mapping the micro-proteome of the nuclear lamina and lamin associated domains
    Article Snippet: .. DamID-seq Library Preparation and Sequencing In order to ensure sequencing of all DamID fragments, post-DamID amplified material was randomized by performing an end repair reaction, followed by ligation and sonication. .. Specifically, 0.5-5 μg of column purified DamID material (from above) was end-repaired using the NEBNext End Repair Module (NEB E6050S) following manufacturer’s recommendations.

    Purification:

    Article Title: SC3-seq: a method for highly parallel and quantitative measurement of single-cell gene expression
    Article Snippet: .. The purified cDNAs were diluted to 130 μl by DDW and fragmented by shearing with Covaris S2 or E210 (duty cycle: 20%; intensity: 5; cycles per burst: 200; duration: 50 s; an intensifier was installed in the case of E210) [Covaris, Woburn, MA, USA] and then end-polished in the End-polish buffer [1 × NEBnext End Repair Reaction buffer [NEB (B6052S), Ipswich, MA, USA], 0.01 U/μl of T4 DNA polymerase [NEB (M0203)] and 0.033 U/μl of T4 polynucleotide kinase [NEB (M0201)]] for 30 min at 20ºC. .. After incubation, a 0.8 × volume of the AMPureXP was immediately added, the solution was mixed for more than 20 min and then the supernatant was transferred to a 1.2 × volume of the AMPureXP reagent and the cDNAs were purified.

    Article Title: CoGAPS matrix factorization algorithm identifies transcriptional changes in AP-2alpha target genes in feedback from therapeutic inhibition of the EGFR network
    Article Snippet: .. Purified, fragmented DNA was mixed with 36 μl of H2O, 10 μl of End Repair Reaction Buffer, 5 μl of End Repair Enzyme Mix (cat# E6050, NEB). .. The 100 μl end-repair mixture was incubated at 20°C for 30 min, and purified using Agencourt AMPure XP beads (Beckman Coulter) in a ratio of 1.0 to 1.25 of PCR product to beads and washed using 70% ethanol per the manufacturer's instructions.

    Incubation:

    Article Title: BisQC: an operational pipeline for multiplexed bisulfite sequencing
    Article Snippet: .. Filling- in of MspI rragmented DNA Using the MspI fragmented DNA (40–85 μL) described above, the NEBNext End Repair Reaction Buffer (10X; 10 μL), and the NEBNext End Repair Enzyme Mix (5 μL), we incubated the solution (final volume of 100 μl) at 20°C for 30 minutes. .. After incubation, the reaction mixture was diluted to 200 μL with dH2 O.

    Article Title: Characterization of the single-cell transcriptional landscape by highly multiplex RNA-seq
    Article Snippet: .. Beads were resuspended in 25 μL EBT, and 25 μL NEBNext End Repair reaction mix (New England Biolabs) was added; then the beads were incubated for 30 min at room temperature. .. The beads were washed twice in EBT, then resuspended in 21 μL EBT; 2.5 μL NEBNext dA tailing buffer and 1.5 μL Klenow exo- (both NEB) were added, and the reaction was incubated for 30 min at 37°C, followed by two washes in 50 μL EBT.

    Sequencing:

    Article Title: Mapping the micro-proteome of the nuclear lamina and lamin associated domains
    Article Snippet: .. DamID-seq Library Preparation and Sequencing In order to ensure sequencing of all DamID fragments, post-DamID amplified material was randomized by performing an end repair reaction, followed by ligation and sonication. .. Specifically, 0.5-5 μg of column purified DamID material (from above) was end-repaired using the NEBNext End Repair Module (NEB E6050S) following manufacturer’s recommendations.

    Sonication:

    Article Title: Mapping the micro-proteome of the nuclear lamina and lamin associated domains
    Article Snippet: .. DamID-seq Library Preparation and Sequencing In order to ensure sequencing of all DamID fragments, post-DamID amplified material was randomized by performing an end repair reaction, followed by ligation and sonication. .. Specifically, 0.5-5 μg of column purified DamID material (from above) was end-repaired using the NEBNext End Repair Module (NEB E6050S) following manufacturer’s recommendations.

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  • 99
    New England Biolabs end prep enzyme mix
    End Prep Enzyme Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/end prep enzyme mix/product/New England Biolabs
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    end prep enzyme mix - by Bioz Stars, 2020-07
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    New England Biolabs neb end prep enzyme mix
    Neb End Prep Enzyme Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neb end prep enzyme mix/product/New England Biolabs
    Average 90 stars, based on 5 article reviews
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    neb end prep enzyme mix - by Bioz Stars, 2020-07
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