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Illumina Inc end adenylation
End Adenylation, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 98/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/end adenylation/product/Illumina Inc
Average 98 stars, based on 19 article reviews
Price from $9.99 to $1999.99
end adenylation - by Bioz Stars, 2020-01
98/100 stars

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DNA Synthesis:

Article Title: Global DNA Hypermethylation in Down Syndrome Placenta
Article Snippet: .. Messenger RNA purification and fragmentation, complementary DNA synthesis, end-repair, 3′-end-adenylation, and adapter-ligation were performed using Illumina's mRNA-Seq Sample Preparation Kit. .. Manufacturer's instructions were followed, except that the SuperScript III First-Strand Synthesis SuperMix (Life Technologies) was used for first strand cDNA synthesis.

RNA Extraction:

Article Title: The Cyclic AMP Receptor Protein Regulates Quorum Sensing and Global Gene Expression in Yersinia pestis during Planktonic Growth and Growth in Biofilms
Article Snippet: Paragraph title: RNA extraction, sequencing, and qRT-PCR. ... This procedure includes depletion of rRNA by the use of a Ribo-Zero rRNA removal kit (bacteria), purification and fragmentation of the remaining RNA, cDNA synthesis, 3′-end adenylation, Illumina adapter ligation, and library PCR amplification and validation.

Amplification:

Article Title: IFNγ-inducible antiviral responses require ULK1-mediated activation of MLK3 and ERK5
Article Snippet: .. This procedure includes mRNA purification and fragmentation, cDNA synthesis, 3’ end adenylation, Illumina adapter ligation, library PCR amplification and validation. .. An Illumina NextSeq 500 Sequencer was used to sequence the libraries with the production of single-end, 75 bp reads.

Article Title: The Cyclic AMP Receptor Protein Regulates Quorum Sensing and Global Gene Expression in Yersinia pestis during Planktonic Growth and Growth in Biofilms
Article Snippet: .. This procedure includes depletion of rRNA by the use of a Ribo-Zero rRNA removal kit (bacteria), purification and fragmentation of the remaining RNA, cDNA synthesis, 3′-end adenylation, Illumina adapter ligation, and library PCR amplification and validation. .. An Illumina NextSeq 500 sequencer was used to sequence the libraries with the production of single-ended, 75-bp reads.

Sample Prep:

Article Title: Global DNA Hypermethylation in Down Syndrome Placenta
Article Snippet: .. Messenger RNA purification and fragmentation, complementary DNA synthesis, end-repair, 3′-end-adenylation, and adapter-ligation were performed using Illumina's mRNA-Seq Sample Preparation Kit. .. Manufacturer's instructions were followed, except that the SuperScript III First-Strand Synthesis SuperMix (Life Technologies) was used for first strand cDNA synthesis.

Article Title: Evidence for Retrovirus and Paramyxovirus Infection of Multiple Bat Species in China
Article Snippet: After the 3'-end adenylation, DNA fragments were ligated with Illumina PE adapters on both sides to purify and selectively enrich the 200 bp fragments using the AMPure XP system (Beckman Coulter, Fullerton, CA, USA) and Illumina PCR Primer Cocktail in a 10 cycle PCR reaction. .. Finally, a sequencing library was generated by Illumina TruSeqTM RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) following the manufacturer’s recommendations and four index codes were added to attribute sequences.

Article Title: Gene expression defines natural changes in mammalian lifespan
Article Snippet: RNA sequencing Sequencing libraries were prepared using the mRNA-Seq Sample Prep Kit Illumina (San Diego, CA. .. Following 3′-end adenylation of these products, Illumina paired-end sequencing adapters were ligated to the blunt ends of the cDNA fragments.

Ligation:

Article Title: IFNγ-inducible antiviral responses require ULK1-mediated activation of MLK3 and ERK5
Article Snippet: .. This procedure includes mRNA purification and fragmentation, cDNA synthesis, 3’ end adenylation, Illumina adapter ligation, library PCR amplification and validation. .. An Illumina NextSeq 500 Sequencer was used to sequence the libraries with the production of single-end, 75 bp reads.

Article Title: The Cyclic AMP Receptor Protein Regulates Quorum Sensing and Global Gene Expression in Yersinia pestis during Planktonic Growth and Growth in Biofilms
Article Snippet: .. This procedure includes depletion of rRNA by the use of a Ribo-Zero rRNA removal kit (bacteria), purification and fragmentation of the remaining RNA, cDNA synthesis, 3′-end adenylation, Illumina adapter ligation, and library PCR amplification and validation. .. An Illumina NextSeq 500 sequencer was used to sequence the libraries with the production of single-ended, 75-bp reads.

Isolation:

Article Title: IFNγ-inducible antiviral responses require ULK1-mediated activation of MLK3 and ERK5
Article Snippet: Total RNA was isolated using the RNeasy Mini Kit (QIAGEN), following the manufacturer’s instructions. .. This procedure includes mRNA purification and fragmentation, cDNA synthesis, 3’ end adenylation, Illumina adapter ligation, library PCR amplification and validation.

Article Title: Gene expression defines natural changes in mammalian lifespan
Article Snippet: Polyadenylated RNA was isolated using a poly-dT bead procedure, chemically fragmented, and randomly primed for reverse transcription. .. Following 3′-end adenylation of these products, Illumina paired-end sequencing adapters were ligated to the blunt ends of the cDNA fragments.

Article Title: The Cyclic AMP Receptor Protein Regulates Quorum Sensing and Global Gene Expression in Yersinia pestis during Planktonic Growth and Growth in Biofilms
Article Snippet: Total RNA was isolated from Y. pestis cultures as described previously ( ) except that RNA protect reagent (Qiagen) was added to planktonic or biofilm cells in PBS. .. This procedure includes depletion of rRNA by the use of a Ribo-Zero rRNA removal kit (bacteria), purification and fragmentation of the remaining RNA, cDNA synthesis, 3′-end adenylation, Illumina adapter ligation, and library PCR amplification and validation.

RNA Sequencing Assay:

Article Title: IFNγ-inducible antiviral responses require ULK1-mediated activation of MLK3 and ERK5
Article Snippet: Paragraph title: Library Construction and RNA-Sequencing (RNA-Seq) ... This procedure includes mRNA purification and fragmentation, cDNA synthesis, 3’ end adenylation, Illumina adapter ligation, library PCR amplification and validation.

Article Title: Gene expression defines natural changes in mammalian lifespan
Article Snippet: Paragraph title: RNA sequencing ... Following 3′-end adenylation of these products, Illumina paired-end sequencing adapters were ligated to the blunt ends of the cDNA fragments.

Article Title: The Cyclic AMP Receptor Protein Regulates Quorum Sensing and Global Gene Expression in Yersinia pestis during Planktonic Growth and Growth in Biofilms
Article Snippet: Next-generation RNA sequencing was carried out at the Northwestern University NUSeq Core Facility. .. This procedure includes depletion of rRNA by the use of a Ribo-Zero rRNA removal kit (bacteria), purification and fragmentation of the remaining RNA, cDNA synthesis, 3′-end adenylation, Illumina adapter ligation, and library PCR amplification and validation.

Quantitative RT-PCR:

Article Title: The Cyclic AMP Receptor Protein Regulates Quorum Sensing and Global Gene Expression in Yersinia pestis during Planktonic Growth and Growth in Biofilms
Article Snippet: Paragraph title: RNA extraction, sequencing, and qRT-PCR. ... This procedure includes depletion of rRNA by the use of a Ribo-Zero rRNA removal kit (bacteria), purification and fragmentation of the remaining RNA, cDNA synthesis, 3′-end adenylation, Illumina adapter ligation, and library PCR amplification and validation.

Purification:

Article Title: Global DNA Hypermethylation in Down Syndrome Placenta
Article Snippet: .. Messenger RNA purification and fragmentation, complementary DNA synthesis, end-repair, 3′-end-adenylation, and adapter-ligation were performed using Illumina's mRNA-Seq Sample Preparation Kit. .. Manufacturer's instructions were followed, except that the SuperScript III First-Strand Synthesis SuperMix (Life Technologies) was used for first strand cDNA synthesis.

Article Title: IFNγ-inducible antiviral responses require ULK1-mediated activation of MLK3 and ERK5
Article Snippet: .. This procedure includes mRNA purification and fragmentation, cDNA synthesis, 3’ end adenylation, Illumina adapter ligation, library PCR amplification and validation. .. An Illumina NextSeq 500 Sequencer was used to sequence the libraries with the production of single-end, 75 bp reads.

Article Title: Gene expression defines natural changes in mammalian lifespan
Article Snippet: Following 3′-end adenylation of these products, Illumina paired-end sequencing adapters were ligated to the blunt ends of the cDNA fragments. .. After column purification, quality of the resulting libraries was assessed using Agilent 2100 Bioanalyzer.

Article Title: The Cyclic AMP Receptor Protein Regulates Quorum Sensing and Global Gene Expression in Yersinia pestis during Planktonic Growth and Growth in Biofilms
Article Snippet: .. This procedure includes depletion of rRNA by the use of a Ribo-Zero rRNA removal kit (bacteria), purification and fragmentation of the remaining RNA, cDNA synthesis, 3′-end adenylation, Illumina adapter ligation, and library PCR amplification and validation. .. An Illumina NextSeq 500 sequencer was used to sequence the libraries with the production of single-ended, 75-bp reads.

Sequencing:

Article Title: Global DNA Hypermethylation in Down Syndrome Placenta
Article Snippet: Paragraph title: mRNA Sequencing (mRNA-SEQ) ... Messenger RNA purification and fragmentation, complementary DNA synthesis, end-repair, 3′-end-adenylation, and adapter-ligation were performed using Illumina's mRNA-Seq Sample Preparation Kit.

Article Title: IFNγ-inducible antiviral responses require ULK1-mediated activation of MLK3 and ERK5
Article Snippet: Sequencing libraries were prepared from 1μg of high-quality RNA samples using Illumina TruSeq Stranded mRNA Library Preparation Kit (Illumina) as per the manufacturer’s instructions. .. This procedure includes mRNA purification and fragmentation, cDNA synthesis, 3’ end adenylation, Illumina adapter ligation, library PCR amplification and validation.

Article Title: Evidence for Retrovirus and Paramyxovirus Infection of Multiple Bat Species in China
Article Snippet: Paragraph title: 2.4. High-throughput Sequencing and Pathogen Analysis ... After the 3'-end adenylation, DNA fragments were ligated with Illumina PE adapters on both sides to purify and selectively enrich the 200 bp fragments using the AMPure XP system (Beckman Coulter, Fullerton, CA, USA) and Illumina PCR Primer Cocktail in a 10 cycle PCR reaction.

Article Title: Gene expression defines natural changes in mammalian lifespan
Article Snippet: .. Following 3′-end adenylation of these products, Illumina paired-end sequencing adapters were ligated to the blunt ends of the cDNA fragments. .. Ligated products were run on gels; 300-bp fragments were excised and then PCR-amplified (15 cycles).

Article Title: The Cyclic AMP Receptor Protein Regulates Quorum Sensing and Global Gene Expression in Yersinia pestis during Planktonic Growth and Growth in Biofilms
Article Snippet: Paragraph title: RNA extraction, sequencing, and qRT-PCR. ... This procedure includes depletion of rRNA by the use of a Ribo-Zero rRNA removal kit (bacteria), purification and fragmentation of the remaining RNA, cDNA synthesis, 3′-end adenylation, Illumina adapter ligation, and library PCR amplification and validation.

Polymerase Chain Reaction:

Article Title: IFNγ-inducible antiviral responses require ULK1-mediated activation of MLK3 and ERK5
Article Snippet: .. This procedure includes mRNA purification and fragmentation, cDNA synthesis, 3’ end adenylation, Illumina adapter ligation, library PCR amplification and validation. .. An Illumina NextSeq 500 Sequencer was used to sequence the libraries with the production of single-end, 75 bp reads.

Article Title: Evidence for Retrovirus and Paramyxovirus Infection of Multiple Bat Species in China
Article Snippet: .. After the 3'-end adenylation, DNA fragments were ligated with Illumina PE adapters on both sides to purify and selectively enrich the 200 bp fragments using the AMPure XP system (Beckman Coulter, Fullerton, CA, USA) and Illumina PCR Primer Cocktail in a 10 cycle PCR reaction. .. Finally, a sequencing library was generated by Illumina TruSeqTM RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) following the manufacturer’s recommendations and four index codes were added to attribute sequences.

Article Title: Gene expression defines natural changes in mammalian lifespan
Article Snippet: Following 3′-end adenylation of these products, Illumina paired-end sequencing adapters were ligated to the blunt ends of the cDNA fragments. .. Ligated products were run on gels; 300-bp fragments were excised and then PCR-amplified (15 cycles).

Article Title: The Cyclic AMP Receptor Protein Regulates Quorum Sensing and Global Gene Expression in Yersinia pestis during Planktonic Growth and Growth in Biofilms
Article Snippet: .. This procedure includes depletion of rRNA by the use of a Ribo-Zero rRNA removal kit (bacteria), purification and fragmentation of the remaining RNA, cDNA synthesis, 3′-end adenylation, Illumina adapter ligation, and library PCR amplification and validation. .. An Illumina NextSeq 500 sequencer was used to sequence the libraries with the production of single-ended, 75-bp reads.

Generated:

Article Title: Evidence for Retrovirus and Paramyxovirus Infection of Multiple Bat Species in China
Article Snippet: After the 3'-end adenylation, DNA fragments were ligated with Illumina PE adapters on both sides to purify and selectively enrich the 200 bp fragments using the AMPure XP system (Beckman Coulter, Fullerton, CA, USA) and Illumina PCR Primer Cocktail in a 10 cycle PCR reaction. .. Finally, a sequencing library was generated by Illumina TruSeqTM RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) following the manufacturer’s recommendations and four index codes were added to attribute sequences.

High Throughput Screening Assay:

Article Title: Evidence for Retrovirus and Paramyxovirus Infection of Multiple Bat Species in China
Article Snippet: Paragraph title: 2.4. High-throughput Sequencing and Pathogen Analysis ... After the 3'-end adenylation, DNA fragments were ligated with Illumina PE adapters on both sides to purify and selectively enrich the 200 bp fragments using the AMPure XP system (Beckman Coulter, Fullerton, CA, USA) and Illumina PCR Primer Cocktail in a 10 cycle PCR reaction.

Agarose Gel Electrophoresis:

Article Title: Global DNA Hypermethylation in Down Syndrome Placenta
Article Snippet: Messenger RNA purification and fragmentation, complementary DNA synthesis, end-repair, 3′-end-adenylation, and adapter-ligation were performed using Illumina's mRNA-Seq Sample Preparation Kit. .. Adapter-ligated cDNA fragments were size-selected using a 3% agarose gel (200±25 bp).

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    Illumina Inc end adenylation
    End Adenylation, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 98/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/end adenylation/product/Illumina Inc
    Average 98 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    end adenylation - by Bioz Stars, 2020-01
    98/100 stars
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