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Illumina Inc end adenylation
End Adenylation, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/end adenylation/product/Illumina Inc
Average 90 stars, based on 29 article reviews
Price from $9.99 to $1999.99
end adenylation - by Bioz Stars, 2020-07
90/100 stars

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Amplification:

Article Title: Disordered chromatin packing regulates phenotypic plasticity
Article Snippet: .. This procedure includes mRNA purification and fragmentation, complementary DNA synthesis, 3′ end adenylation, Illumina adapter ligation, library polymerase chain reaction (PCR) amplification, and validation. .. Illumina HiSeq 4000 Sequencer was used to sequence the libraries with the production of single-end, 50–base pair reads.

Article Title: IFNγ-inducible antiviral responses require ULK1-mediated activation of MLK3 and ERK5
Article Snippet: .. This procedure includes mRNA purification and fragmentation, cDNA synthesis, 3’ end adenylation, Illumina adapter ligation, library PCR amplification and validation. .. An Illumina NextSeq 500 Sequencer was used to sequence the libraries with the production of single-end, 75 bp reads.

Sample Prep:

Article Title: A complex association between DNA methylation and gene expression in human placenta at first and third trimesters
Article Snippet: .. Messenger RNA purification and fragmentation, complementary DNA synthesis, end-repair, 3’-end-adenylation, and adapter-ligation were performed using Illumina’s mRNA-Seq Sample Preparation Kit. .. Manufacturer’s instructions were followed, except that the SuperScript III First-Strand Synthesis SuperMix (Life Technologies) was used for first strand cDNA synthesis.

Ligation:

Article Title: Identification of aberrantly expressed long non-coding RNAs in stomach adenocarcinoma
Article Snippet: .. RNA was purified and fragmented into 200 base pairs; RNA fragment primed with random hexamer primers were applied for the first cDNA strand synthesis; then the second cDNA strand was synthesized and dUTP was used instead of dTTP; the double cDNA strands were underwent end pair, 3′end adenylation and adapter ligation; the second cDNA strand was digested by UNG enzyme (Illumina, Inc., San Diego, CA, USA). .. Thirdly, libraries were amplified through polymerase chain reaction for 15 cycles, purified through Certified Low Range Ultra Agarose (Bio-Rad) and quantified through Picogreen (Molecular probes) on TBS380 (Turner Biosystems).

Article Title: Disordered chromatin packing regulates phenotypic plasticity
Article Snippet: .. This procedure includes mRNA purification and fragmentation, complementary DNA synthesis, 3′ end adenylation, Illumina adapter ligation, library polymerase chain reaction (PCR) amplification, and validation. .. Illumina HiSeq 4000 Sequencer was used to sequence the libraries with the production of single-end, 50–base pair reads.

Article Title: IFNγ-inducible antiviral responses require ULK1-mediated activation of MLK3 and ERK5
Article Snippet: .. This procedure includes mRNA purification and fragmentation, cDNA synthesis, 3’ end adenylation, Illumina adapter ligation, library PCR amplification and validation. .. An Illumina NextSeq 500 Sequencer was used to sequence the libraries with the production of single-end, 75 bp reads.

Article Title: Hypoxia causes transgenerational impairments in reproduction of fish
Article Snippet: .. Overhangs were blunted by treatment with exonuclease/polymerase followed by 3′-end adenylation and ligation to Illumina PE adaptor oligonucleotides. .. DNA fragments successfully ligated with adaptor molecules on both ends were enriched using the Illumina PCR Primer Cocktail in a 15 cycle PCR reaction.

Article Title: Hepatocyte nuclear factor 4α negatively regulates connective tissue growth factor during liver regeneration.
Article Snippet: .. End repair, 3′ end adenylation and the barcoded adapters (Illumina) were performed to the fragmented cDNA in prior to ligation with Kapa LT library preparation kit (Kapa Biosystems, Wilmington, MA). .. The prepared libraries were validated using a 2100 Bioanalyzer DNA High Sensitivity chip, and quantified by Qubit Fluorometric Quantitation (Waltham, MA).

Synthesized:

Article Title: Identification of aberrantly expressed long non-coding RNAs in stomach adenocarcinoma
Article Snippet: .. RNA was purified and fragmented into 200 base pairs; RNA fragment primed with random hexamer primers were applied for the first cDNA strand synthesis; then the second cDNA strand was synthesized and dUTP was used instead of dTTP; the double cDNA strands were underwent end pair, 3′end adenylation and adapter ligation; the second cDNA strand was digested by UNG enzyme (Illumina, Inc., San Diego, CA, USA). .. Thirdly, libraries were amplified through polymerase chain reaction for 15 cycles, purified through Certified Low Range Ultra Agarose (Bio-Rad) and quantified through Picogreen (Molecular probes) on TBS380 (Turner Biosystems).

Purification:

Article Title: Identification of aberrantly expressed long non-coding RNAs in stomach adenocarcinoma
Article Snippet: .. RNA was purified and fragmented into 200 base pairs; RNA fragment primed with random hexamer primers were applied for the first cDNA strand synthesis; then the second cDNA strand was synthesized and dUTP was used instead of dTTP; the double cDNA strands were underwent end pair, 3′end adenylation and adapter ligation; the second cDNA strand was digested by UNG enzyme (Illumina, Inc., San Diego, CA, USA). .. Thirdly, libraries were amplified through polymerase chain reaction for 15 cycles, purified through Certified Low Range Ultra Agarose (Bio-Rad) and quantified through Picogreen (Molecular probes) on TBS380 (Turner Biosystems).

Article Title: A complex association between DNA methylation and gene expression in human placenta at first and third trimesters
Article Snippet: .. Messenger RNA purification and fragmentation, complementary DNA synthesis, end-repair, 3’-end-adenylation, and adapter-ligation were performed using Illumina’s mRNA-Seq Sample Preparation Kit. .. Manufacturer’s instructions were followed, except that the SuperScript III First-Strand Synthesis SuperMix (Life Technologies) was used for first strand cDNA synthesis.

Article Title: Disordered chromatin packing regulates phenotypic plasticity
Article Snippet: .. This procedure includes mRNA purification and fragmentation, complementary DNA synthesis, 3′ end adenylation, Illumina adapter ligation, library polymerase chain reaction (PCR) amplification, and validation. .. Illumina HiSeq 4000 Sequencer was used to sequence the libraries with the production of single-end, 50–base pair reads.

Article Title: IFNγ-inducible antiviral responses require ULK1-mediated activation of MLK3 and ERK5
Article Snippet: .. This procedure includes mRNA purification and fragmentation, cDNA synthesis, 3’ end adenylation, Illumina adapter ligation, library PCR amplification and validation. .. An Illumina NextSeq 500 Sequencer was used to sequence the libraries with the production of single-end, 75 bp reads.

Polymerase Chain Reaction:

Article Title: Disordered chromatin packing regulates phenotypic plasticity
Article Snippet: .. This procedure includes mRNA purification and fragmentation, complementary DNA synthesis, 3′ end adenylation, Illumina adapter ligation, library polymerase chain reaction (PCR) amplification, and validation. .. Illumina HiSeq 4000 Sequencer was used to sequence the libraries with the production of single-end, 50–base pair reads.

Article Title: IFNγ-inducible antiviral responses require ULK1-mediated activation of MLK3 and ERK5
Article Snippet: .. This procedure includes mRNA purification and fragmentation, cDNA synthesis, 3’ end adenylation, Illumina adapter ligation, library PCR amplification and validation. .. An Illumina NextSeq 500 Sequencer was used to sequence the libraries with the production of single-end, 75 bp reads.

Article Title: Role of FEN1 S187 phosphorylation in counteracting oxygen-induced stress and regulating postnatal heart development
Article Snippet: .. The fragmented cDNA underwent end repair and 3′ end adenylation, and finally the bar-coded adapters (Illumina) were ligated to the cDNA fragments, and 10 cycles of PCR were performed to produce the final sequencing library. .. Library templates were prepared for sequencing using the cBot cluster generation system with the HiSeq SR Cluster Kit V4 (Illumina).

Article Title: Evidence for Retrovirus and Paramyxovirus Infection of Multiple Bat Species in China
Article Snippet: .. After the 3'-end adenylation, DNA fragments were ligated with Illumina PE adapters on both sides to purify and selectively enrich the 200 bp fragments using the AMPure XP system (Beckman Coulter, Fullerton, CA, USA) and Illumina PCR Primer Cocktail in a 10 cycle PCR reaction. .. Finally, a sequencing library was generated by Illumina TruSeqTM RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) following the manufacturer’s recommendations and four index codes were added to attribute sequences.

Random Hexamer Labeling:

Article Title: Identification of aberrantly expressed long non-coding RNAs in stomach adenocarcinoma
Article Snippet: .. RNA was purified and fragmented into 200 base pairs; RNA fragment primed with random hexamer primers were applied for the first cDNA strand synthesis; then the second cDNA strand was synthesized and dUTP was used instead of dTTP; the double cDNA strands were underwent end pair, 3′end adenylation and adapter ligation; the second cDNA strand was digested by UNG enzyme (Illumina, Inc., San Diego, CA, USA). .. Thirdly, libraries were amplified through polymerase chain reaction for 15 cycles, purified through Certified Low Range Ultra Agarose (Bio-Rad) and quantified through Picogreen (Molecular probes) on TBS380 (Turner Biosystems).

Sequencing:

Article Title: Role of FEN1 S187 phosphorylation in counteracting oxygen-induced stress and regulating postnatal heart development
Article Snippet: .. The fragmented cDNA underwent end repair and 3′ end adenylation, and finally the bar-coded adapters (Illumina) were ligated to the cDNA fragments, and 10 cycles of PCR were performed to produce the final sequencing library. .. Library templates were prepared for sequencing using the cBot cluster generation system with the HiSeq SR Cluster Kit V4 (Illumina).

DNA Synthesis:

Article Title: A complex association between DNA methylation and gene expression in human placenta at first and third trimesters
Article Snippet: .. Messenger RNA purification and fragmentation, complementary DNA synthesis, end-repair, 3’-end-adenylation, and adapter-ligation were performed using Illumina’s mRNA-Seq Sample Preparation Kit. .. Manufacturer’s instructions were followed, except that the SuperScript III First-Strand Synthesis SuperMix (Life Technologies) was used for first strand cDNA synthesis.

Article Title: Disordered chromatin packing regulates phenotypic plasticity
Article Snippet: .. This procedure includes mRNA purification and fragmentation, complementary DNA synthesis, 3′ end adenylation, Illumina adapter ligation, library polymerase chain reaction (PCR) amplification, and validation. .. Illumina HiSeq 4000 Sequencer was used to sequence the libraries with the production of single-end, 50–base pair reads.

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    Illumina Inc end adenylation
    End Adenylation, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/end adenylation/product/Illumina Inc
    Average 90 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    end adenylation - by Bioz Stars, 2020-07
    90/100 stars
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