dnase  (Jena Bioscience)


Bioz Verified Symbol Jena Bioscience is a verified supplier
Bioz Manufacturer Symbol Jena Bioscience manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    DNase I
    Description:

    Catalog Number:
    EN-173L
    Price:
    205.7
    Category:
    Molecular Biology
    Size:
    5 x 2000 units
    Buy from Supplier


    Structured Review

    Jena Bioscience dnase

    https://www.bioz.com/result/dnase/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase - by Bioz Stars, 2021-06
    93/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Design and Development of Modified mRNA Encoding Core Antigen of Hepatitis C Virus: a Possible Application in Vaccine Production
    Article Snippet: .. To remove the template DNA, 1 μl of RQ1 RNase-Free DNase (Catalog Numbers: M6101) was added to the IVT reaction mixture and incubated at 37°C for 15 min. Purification of mRNA was performed after DNaseI digestion by Total RNA Purification Kit (Jena-Bioscience , Germany), according to the instructions provided by manufacturer. .. The purity and quality of the RNAs were determined using spectrophotometric analysis and run on the agarose gel (1.1%).

    Article Title: Enzyme catalysis prior to aromatic residues: reverse engineering of a dephosphoCoA kinase
    Article Snippet: When OD600 reached 0.7-0.8, isopropyl β-D-thiogalactopyranoside (IPTG, Sigma Aldrich, USA) was added to final concentration of 0.5 mM and the cultivation was continued for 4 h at 37 °C. .. The cell pellets were resuspended in 15 ml of lysis buffer (20 mM Tris (pH 8.0), 20 mM NaCl, and 1 mM of β-mercaptoethanol) with one tablet of EASYpack protease inhibitor cocktail (Sigma Aldrich, USA), incubated with 50 μg/ml of lysozyme and 6 U of RNase-free DNase I (Jena Bioscience, Germany) at room temperature for 30 min, sonicated on ice at 1.5 W (18 cycles, 10 sec on, 20 sec off) and centrifuged at 35000× g for 30 min at 4 °C. .. After, Tween-20 (Sigma Aldrich, USA) was added to supernatants to the final concentration of 0.1 % (v/v), and the crude lysates were applied to 5 ml HiTrap Capto Q column (GE Healthcare Life Sciences, USA) equilibrated with 5 volumes of buffer A (20 mM Tris (pH 8.0), 20 mM NaCl, 1 mM beta-mercaptoethanol and 0.1 % (v/v) Tween-20).

    Purification:

    Article Title: Design and Development of Modified mRNA Encoding Core Antigen of Hepatitis C Virus: a Possible Application in Vaccine Production
    Article Snippet: .. To remove the template DNA, 1 μl of RQ1 RNase-Free DNase (Catalog Numbers: M6101) was added to the IVT reaction mixture and incubated at 37°C for 15 min. Purification of mRNA was performed after DNaseI digestion by Total RNA Purification Kit (Jena-Bioscience , Germany), according to the instructions provided by manufacturer. .. The purity and quality of the RNAs were determined using spectrophotometric analysis and run on the agarose gel (1.1%).

    Concentration Assay:

    Article Title: The role of β-d-mannuronic acid, as a new non-steroidal anti-inflammatory drug on expression of miR-146a, IRAK1, TRAF6, NF-κB and pro-inflammatory cytokines following a clinical trial in rheumatoid arthritis patients.
    Article Snippet: < b > Context: < /b > miR-146a, its targets (IRAK1, TRAF6) and NF-κB transcription factor play a fundamental role in rheumatoid arthritis (RA). .. < b > Context: < /b > miR-146a, its targets (IRAK1, TRAF6) and NF-κB transcription factor play a fundamental role in rheumatoid arthritis (RA). .. < b > Context: < /b > miR-146a, its targets (IRAK1, TRAF6) and NF-κB transcription factor play a fundamental role in rheumatoid arthritis (RA).

    Article Title: The Situation of Chemokine Ligands and Receptors Gene Expression, Following the Oral Administration of Drug Mannuronic Acid in Rheumatoid Arthritis Patients.
    Article Snippet: Regarding the leukocytes infiltration into the synovium of Rheumatoid Arthritis (RA) patients is mostly mediated by chemokine ligands and receptors, and following the efficient and motivating results of international Phase III clinical trial of β-D-Mannuronic acid (M2000) patented EP067919 (2017), as a novel anti-inflammatory drug, in patients with RA, the present research was designed. .. Regarding the leukocytes infiltration into the synovium of Rheumatoid Arthritis (RA) patients is mostly mediated by chemokine ligands and receptors, and following the efficient and motivating results of international Phase III clinical trial of β-D-Mannuronic acid (M2000) patented EP067919 (2017), as a novel anti-inflammatory drug, in patients with RA, the present research was designed. .. Regarding the leukocytes infiltration into the synovium of Rheumatoid Arthritis (RA) patients is mostly mediated by chemokine ligands and receptors, and following the efficient and motivating results of international Phase III clinical trial of β-D-Mannuronic acid (M2000) patented EP067919 (2017), as a novel anti-inflammatory drug, in patients with RA, the present research was designed.

    Lysis:

    Article Title: Enzyme catalysis prior to aromatic residues: reverse engineering of a dephosphoCoA kinase
    Article Snippet: When OD600 reached 0.7-0.8, isopropyl β-D-thiogalactopyranoside (IPTG, Sigma Aldrich, USA) was added to final concentration of 0.5 mM and the cultivation was continued for 4 h at 37 °C. .. The cell pellets were resuspended in 15 ml of lysis buffer (20 mM Tris (pH 8.0), 20 mM NaCl, and 1 mM of β-mercaptoethanol) with one tablet of EASYpack protease inhibitor cocktail (Sigma Aldrich, USA), incubated with 50 μg/ml of lysozyme and 6 U of RNase-free DNase I (Jena Bioscience, Germany) at room temperature for 30 min, sonicated on ice at 1.5 W (18 cycles, 10 sec on, 20 sec off) and centrifuged at 35000× g for 30 min at 4 °C. .. After, Tween-20 (Sigma Aldrich, USA) was added to supernatants to the final concentration of 0.1 % (v/v), and the crude lysates were applied to 5 ml HiTrap Capto Q column (GE Healthcare Life Sciences, USA) equilibrated with 5 volumes of buffer A (20 mM Tris (pH 8.0), 20 mM NaCl, 1 mM beta-mercaptoethanol and 0.1 % (v/v) Tween-20).

    Protease Inhibitor:

    Article Title: Enzyme catalysis prior to aromatic residues: reverse engineering of a dephosphoCoA kinase
    Article Snippet: When OD600 reached 0.7-0.8, isopropyl β-D-thiogalactopyranoside (IPTG, Sigma Aldrich, USA) was added to final concentration of 0.5 mM and the cultivation was continued for 4 h at 37 °C. .. The cell pellets were resuspended in 15 ml of lysis buffer (20 mM Tris (pH 8.0), 20 mM NaCl, and 1 mM of β-mercaptoethanol) with one tablet of EASYpack protease inhibitor cocktail (Sigma Aldrich, USA), incubated with 50 μg/ml of lysozyme and 6 U of RNase-free DNase I (Jena Bioscience, Germany) at room temperature for 30 min, sonicated on ice at 1.5 W (18 cycles, 10 sec on, 20 sec off) and centrifuged at 35000× g for 30 min at 4 °C. .. After, Tween-20 (Sigma Aldrich, USA) was added to supernatants to the final concentration of 0.1 % (v/v), and the crude lysates were applied to 5 ml HiTrap Capto Q column (GE Healthcare Life Sciences, USA) equilibrated with 5 volumes of buffer A (20 mM Tris (pH 8.0), 20 mM NaCl, 1 mM beta-mercaptoethanol and 0.1 % (v/v) Tween-20).

    Sonication:

    Article Title: Enzyme catalysis prior to aromatic residues: reverse engineering of a dephosphoCoA kinase
    Article Snippet: When OD600 reached 0.7-0.8, isopropyl β-D-thiogalactopyranoside (IPTG, Sigma Aldrich, USA) was added to final concentration of 0.5 mM and the cultivation was continued for 4 h at 37 °C. .. The cell pellets were resuspended in 15 ml of lysis buffer (20 mM Tris (pH 8.0), 20 mM NaCl, and 1 mM of β-mercaptoethanol) with one tablet of EASYpack protease inhibitor cocktail (Sigma Aldrich, USA), incubated with 50 μg/ml of lysozyme and 6 U of RNase-free DNase I (Jena Bioscience, Germany) at room temperature for 30 min, sonicated on ice at 1.5 W (18 cycles, 10 sec on, 20 sec off) and centrifuged at 35000× g for 30 min at 4 °C. .. After, Tween-20 (Sigma Aldrich, USA) was added to supernatants to the final concentration of 0.1 % (v/v), and the crude lysates were applied to 5 ml HiTrap Capto Q column (GE Healthcare Life Sciences, USA) equilibrated with 5 volumes of buffer A (20 mM Tris (pH 8.0), 20 mM NaCl, 1 mM beta-mercaptoethanol and 0.1 % (v/v) Tween-20).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Establishment and Validation of Theileria annulata Sporozoite Ak-93 Infection in Laboratory-Reared Hyalomma anatolicum Tick Using In Vivo and In Vitro Assays
    Article Snippet: RNA extraction and cDNA synthesis Total RNA was extracted from the tick stabilate using the One Step RNA reagent (YTzol Pure RNA, Yekta Tajhiz Azma, Iran) in accordance with the manufacturer’s instruction. .. DNase treatment was done to assure the RNA response in RT-PCR assay (DNase I, Jena Bioscience, Germany). .. The cDNA synthesis and further PCR were performed using the MMuLV reverse transcriptase and PCR mix (Yekta Tajhiz Azma, Iran) according to the manufacturer’s instructions.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Jena Bioscience dnase treatment
    Dnase Treatment, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase treatment/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase treatment - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    Image Search Results