emv free rpmi 1640  (Millipore)


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    Name:
    RPMI 1640 Vitamins Solution
    Description:
    Roswell Park Memorial Institute medium is commonly referred as RPMI It is a form of medium used in cell culture and tissue culture This medium contains phosphate and is formulated for use in a 5 carbon dioxide atmosphere RPMI 1640 is used for the serum free expansion of human lymphoid cells
    Catalog Number:
    r7256
    Price:
    None
    Applications:
    RPMI 1640 Vitamins Solution (100x) is used as a supplement in cell culture media.
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    Structured Review

    Millipore emv free rpmi 1640
    Roswell Park Memorial Institute medium is commonly referred as RPMI It is a form of medium used in cell culture and tissue culture This medium contains phosphate and is formulated for use in a 5 carbon dioxide atmosphere RPMI 1640 is used for the serum free expansion of human lymphoid cells
    https://www.bioz.com/result/emv free rpmi 1640/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    emv free rpmi 1640 - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Functional characterization of the nitrogen permease regulator-like-2 candidate tumor suppressor gene in colorectal cancer cell lines
    Article Snippet: .. Following culture in RPMI 1640 media, supplemented with 10% FBS at 37°C and 5% CO2 for 2 weeks, the cells were washed twice with phosphate-buffered saline (PBS) and stained with Giemsa (Sigma-Aldrich), following which the number of colonies containing > 50 cells were counted. .. The cloning efficiency was calculated as follows: Cloning efficiency (%) = (number of clones / number of seed cells) × 100%.

    Incubation:

    Article Title: Neutrophil Elastase Subverts the Immune Response by Cleaving Toll-Like Receptors and Cytokines in Pneumococcal Pneumonia
    Article Snippet: .. After 48 h incubation, the cells were washed with RPMI 1640 and cultured further in RPMI 1640 for 12 h. Then, cells were stimulated with heat-killed S. pneumoniae (HK-Spn) or Escherichia coli lipopolysaccharide (LPS) (100 ng/mL; Sigma-Aldrich, St. Louis, MO, USA) in the presence or absence of human neutrophil elastase (hNE; Innovative Research, Novi, MI, USA) and the NE inhibitor sivelestat (100 µg/mL; ONO Pharmaceutical Co., Osaka, Japan) for 3 h under serum-free conditions. .. Gene transcription in THP-1-derived macrophages was quantified using quantitative real-time PCR.

    Cell Culture:

    Article Title: The Step Further to Understand the Role of Cytosolic Phospholipase A2 Alpha and Group X Secretory Phospholipase A2 in Allergic Inflammation: Pilot Study
    Article Snippet: .. Cells were cultured in RPMI1640 (Sigma-Aldrich, St. Louis, MO) with 10% heat-inactivated FBS (Sigma-Aldrich, St. Louis, MO) and antibiotics. .. 10 ng/mL of polymyxin B was added to medium used in allergen stimulation and 100 U/mL penicillin and 100 μ g/mL streptomycin (Sigma-Aldrich, St. Louis, MO) for LPS incubation.

    Article Title: Dehydroxymethylepoxyquinomicin (DHMEQ) can suppress tumour necrosis factor-? production in lipopolysaccharide-injected mice, resulting in rescuing mice from death in vivo
    Article Snippet: .. Spleen cells from B6 mice and W/BF1 mice were cultured in 10% FCS containing RPMI-1640 with LPS (1 mg/ml) with or without Brefeldin A (10 mg/ml; Sigma). .. First, surface staining was performed with fluorescein isothiocyanate (FITC)-labelled anti-CD11c, peridinin chlorophyll (PerCP) Cy5·5-labelled anti-CD45 and allophycocyanin (APC)-labelled anti-CD11b antibodies (BD Bioscience Pharmingen, San Jose, CA, USA).

    Article Title: Cinnamomum cassia Suppresses Caspase-9 through Stimulation of AKT1 in MCF-7 Cells but Not in MDA-MB-231 Cells
    Article Snippet: .. The cell lines were cultured in RPMI-1640 and DMEM (Sigma Aldrich Chemical Company, UK), respectively, and supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin solution at 37°C in a 5% CO2 incubator. .. Cells were washed with PBS (phosphate buffered saline, pH 7.4) and incubated in fresh medium containing different concentrations of the C . cassia extract (CE).

    Article Title: Differential Reactions of Microglia to Brain Metastasis of Lung Cancer
    Article Snippet: .. The cells were cultured in RPMI 1640 (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Hyclone, Germany), 1% antibiotics, 1% l -glutamine, and 1% of 10 mM Hepes buffer. .. Murine immortalized microglial BV-2 cells ( , ) were routinely maintained at 37 °C and 5% CO2 in DMEM (Sigma) supplemented with 10% heat-inactivated FBS.

    Article Title: Neutrophil Elastase Subverts the Immune Response by Cleaving Toll-Like Receptors and Cytokines in Pneumococcal Pneumonia
    Article Snippet: .. After 48 h incubation, the cells were washed with RPMI 1640 and cultured further in RPMI 1640 for 12 h. Then, cells were stimulated with heat-killed S. pneumoniae (HK-Spn) or Escherichia coli lipopolysaccharide (LPS) (100 ng/mL; Sigma-Aldrich, St. Louis, MO, USA) in the presence or absence of human neutrophil elastase (hNE; Innovative Research, Novi, MI, USA) and the NE inhibitor sivelestat (100 µg/mL; ONO Pharmaceutical Co., Osaka, Japan) for 3 h under serum-free conditions. .. Gene transcription in THP-1-derived macrophages was quantified using quantitative real-time PCR.

    Infection:

    Article Title: Lutzomyia longipalpis Saliva Drives Interleukin-17-Induced Neutrophil Recruitment Favoring Leishmania infantum Infection
    Article Snippet: .. Assessment of Intracellular Load of L. infantum Human neutrophils were treated or not with Lu. longipalpis SGS and infected with stationary-phase L. infantum (1 cell:5 parasites) for 3 h. After washing to remove non-internalized parasites, RPMI-1640 media was replaced for Schneider’s insect medium (Sigma Chemical Co., United States), supplemented with 10% heat-inactivated fetal bovine serum (Gibco, United States), L -glutamine (2 mM), penicillin (100 U/ml), streptomycin (100 mg/ml) (Invitrogen, United States) at 23°C for 24 h. Subsequently, the number of viable L. infantum were determined by counting live promastigotes using a Neubauer chamber. ..

    Mouse Assay:

    Article Title: Dehydroxymethylepoxyquinomicin (DHMEQ) can suppress tumour necrosis factor-? production in lipopolysaccharide-injected mice, resulting in rescuing mice from death in vivo
    Article Snippet: .. Spleen cells from B6 mice and W/BF1 mice were cultured in 10% FCS containing RPMI-1640 with LPS (1 mg/ml) with or without Brefeldin A (10 mg/ml; Sigma). .. First, surface staining was performed with fluorescein isothiocyanate (FITC)-labelled anti-CD11c, peridinin chlorophyll (PerCP) Cy5·5-labelled anti-CD45 and allophycocyanin (APC)-labelled anti-CD11b antibodies (BD Bioscience Pharmingen, San Jose, CA, USA).