Journal: ACS Omega

Article Title: Improved Frontal Gel Filtration Chromatography to Examine the Interaction between Small Molecules and a Protein with Multiple Specific Binding Sites

doi: 10.1021/acsomega.4c07939

Figure Lengend Snippet: Schematic diagram of the microbore FGC system. A 250 μL aliquot of a protein–ligand mixture was loaded into the sample loop. In this figure, the valve is at the load position. The HPLC pump used has a mechanism to reduce the fluctuation in pressure by adjusting the motion of the two plungers.

Article Snippet: Empty stainless steel microbore columns (1/8-in. outer diameter, inner diameter 0.7, 1.0, or 1.5 mm, and 250 mm length) were obtained from GL Sciences (Tokyo, Japan).

Techniques:

Journal: ACS Omega

Article Title: Improved Frontal Gel Filtration Chromatography to Examine the Interaction between Small Molecules and a Protein with Multiple Specific Binding Sites

doi: 10.1021/acsomega.4c07939

Figure Lengend Snippet: Binding Models Best-Fit to the Binding Curves Obtained by Microbore FGC a

Article Snippet: Empty stainless steel microbore columns (1/8-in. outer diameter, inner diameter 0.7, 1.0, or 1.5 mm, and 250 mm length) were obtained from GL Sciences (Tokyo, Japan).

Techniques: Binding Assay