elongase long range pcr enzyme kit  (Thermo Fisher)

 
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    Name:
    Long PCR Enzyme Mix
    Description:

    Catalog Number:
    k0181
    Price:
    None
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    Structured Review

    Thermo Fisher elongase long range pcr enzyme kit

    https://www.bioz.com/result/elongase long range pcr enzyme kit/product/Thermo Fisher
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    elongase long range pcr enzyme kit - by Bioz Stars, 2021-02
    94/100 stars

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    Related Articles

    Amplification:

    Article Title: Separation of 1-23-kb complementary DNA strands by urea-agarose gel electrophoresis
    Article Snippet: .. PCR amplification of S. cerevisae rDNA segments PCR was performed using 1.25 U of the Long PCR Enzyme mix (Fermentas) in 50 μl of 1× buffer supplemented with 1.5 mM MgCl2 , containing, 20 pmol of each primer (Integrated DNA Technologies, Coralville, IA, USA), the dNTPs (Promega) at 0.25 mM concentration and 300 ng S. cerevisae genomic DNA prepared as described earlier ( ). ..

    Article Title: Isolation and Characterization of AGAMOUS-Like Genes Associated With Double-Flower Morphogenesis in Kerria japonica (Rosaceae)
    Article Snippet: .. The Sf-KjAG and Df-KjAG genomic DNA was amplified using Long PCR Enzyme Mix (Fermentas, Lithuania). ..

    Article Title: Identification and Heterologous Expression of Genes Involved in Anaerobic Dissimilatory Phosphite Oxidation by Desulfotignum phosphitoxidans ▿
    Article Snippet: .. A 4,998-bp-long fragment, ptxD-ptdFCG , was amplified from D. phosphitoxidans genomic DNA via long PCR with the long PCR enzyme mixture (Fermentas, Germany) and the following primers: F766 (5′-GAAAACCTGGGCATATCAATTTGCGGAGAA-3′) and R5764 (5′-AGCACCTTGGCCCAATAAACACTTGTCAGG-3′). .. The amplified product was purified and cloned into the pGemT Easy vector (Promega, Madison, WI) and transformed into E. coli NEB 10-beta cells.

    Article Title: Wnt/β-catenin signalling is necessary for gut differentiation in a marine annelid, Platynereis dumerilii
    Article Snippet: .. The C-clamp (−) isoform, X7, of Pdu - Tcf cDNA was amplified by polymerase chain reaction using Long PCR Enzyme Mix (Thermo Fisher Scientific#K0182), forward primer TcfPlatyRT-1 (5′-GGGAGATTTTCATGGCGGATTCA-3′) and the reverse primer TcfPlatyRT-4 (5′-CAGTTAGATCAAGCAGAGGTCAGAAGTAATACC-3′) on mixed stage Platynereis cDNA as a template. cDNA was synthesized using SuperScript™ II RT (Invitrogen 18064014) and random hexamer primers (Invitrogen 48190-011) following the manufacturer’s protocol from mRNA isolated with TRIzol™ Reagent (Invitrogen 15596026) according to manufacturer’s instructions. .. Conditions for PCR were as follows: initial denaturation 95 °C/2 min followed by 30 cycles of 95 °C/20 s denaturation, 61 °C/30 s annealing and at 68 °C/3-min extension, closed by an additional extension period of 10 min.

    Synthesized:

    Article Title: Wnt/β-catenin signalling is necessary for gut differentiation in a marine annelid, Platynereis dumerilii
    Article Snippet: .. The C-clamp (−) isoform, X7, of Pdu - Tcf cDNA was amplified by polymerase chain reaction using Long PCR Enzyme Mix (Thermo Fisher Scientific#K0182), forward primer TcfPlatyRT-1 (5′-GGGAGATTTTCATGGCGGATTCA-3′) and the reverse primer TcfPlatyRT-4 (5′-CAGTTAGATCAAGCAGAGGTCAGAAGTAATACC-3′) on mixed stage Platynereis cDNA as a template. cDNA was synthesized using SuperScript™ II RT (Invitrogen 18064014) and random hexamer primers (Invitrogen 48190-011) following the manufacturer’s protocol from mRNA isolated with TRIzol™ Reagent (Invitrogen 15596026) according to manufacturer’s instructions. .. Conditions for PCR were as follows: initial denaturation 95 °C/2 min followed by 30 cycles of 95 °C/20 s denaturation, 61 °C/30 s annealing and at 68 °C/3-min extension, closed by an additional extension period of 10 min.

    Isolation:

    Article Title: Wnt/β-catenin signalling is necessary for gut differentiation in a marine annelid, Platynereis dumerilii
    Article Snippet: .. The C-clamp (−) isoform, X7, of Pdu - Tcf cDNA was amplified by polymerase chain reaction using Long PCR Enzyme Mix (Thermo Fisher Scientific#K0182), forward primer TcfPlatyRT-1 (5′-GGGAGATTTTCATGGCGGATTCA-3′) and the reverse primer TcfPlatyRT-4 (5′-CAGTTAGATCAAGCAGAGGTCAGAAGTAATACC-3′) on mixed stage Platynereis cDNA as a template. cDNA was synthesized using SuperScript™ II RT (Invitrogen 18064014) and random hexamer primers (Invitrogen 48190-011) following the manufacturer’s protocol from mRNA isolated with TRIzol™ Reagent (Invitrogen 15596026) according to manufacturer’s instructions. .. Conditions for PCR were as follows: initial denaturation 95 °C/2 min followed by 30 cycles of 95 °C/20 s denaturation, 61 °C/30 s annealing and at 68 °C/3-min extension, closed by an additional extension period of 10 min.

    Polymerase Chain Reaction:

    Article Title: Mu-driven transposition of recombinant mini-Mu unit DNA in the Corynebacterium glutamicum chromosome
    Article Snippet: .. Restriction enzymes, T4 DNA ligase, Long PCR Enzyme Mix, and High Fidelity PCR Enzyme Mix were purchased from Thermo Fisher Scientific (Waltham, MA, USA) and Taq DNA polymerase was purchased from Sileks-M (Moscow, Russia). .. DNA sequencing was performed commercially by Genotekhnologiya (Moscow, Russia).

    Article Title: Radical-scavenging and Pro-/anti-inflammatory Activity of Tetracycline and Related Phenolic Compounds With or Without Visible Light Irradiation
    Article Snippet: .. TaqMan probes/primers for Cox2 , Nos2 , Tnf α , Ho-1 and 18s rRNA and the PCR enzyme mix for real-time PCR were purchased from Life Technologies Japan. .. Real-time PCR quantification was performed in triplicate using the GeneAmp Sequence Detection System 5700 software (Life Technologies, Japan) in accordance with the manufacturer’s instructions.

    Article Title: A threshold level of NFATc1 activity facilitates thymocyte differentiation and opposes notch-driven leukaemia development
    Article Snippet: .. RT–PCRs to check the expression of six Nfatc1 isoform were performed using the Fermentas Long distance PCR Kit (Cat. # K0181). ..

    Article Title: Separation of 1-23-kb complementary DNA strands by urea-agarose gel electrophoresis
    Article Snippet: .. PCR amplification of S. cerevisae rDNA segments PCR was performed using 1.25 U of the Long PCR Enzyme mix (Fermentas) in 50 μl of 1× buffer supplemented with 1.5 mM MgCl2 , containing, 20 pmol of each primer (Integrated DNA Technologies, Coralville, IA, USA), the dNTPs (Promega) at 0.25 mM concentration and 300 ng S. cerevisae genomic DNA prepared as described earlier ( ). ..

    Article Title: Isolation and Characterization of AGAMOUS-Like Genes Associated With Double-Flower Morphogenesis in Kerria japonica (Rosaceae)
    Article Snippet: .. The Sf-KjAG and Df-KjAG genomic DNA was amplified using Long PCR Enzyme Mix (Fermentas, Lithuania). ..

    Article Title: Identification and Heterologous Expression of Genes Involved in Anaerobic Dissimilatory Phosphite Oxidation by Desulfotignum phosphitoxidans ▿
    Article Snippet: .. A 4,998-bp-long fragment, ptxD-ptdFCG , was amplified from D. phosphitoxidans genomic DNA via long PCR with the long PCR enzyme mixture (Fermentas, Germany) and the following primers: F766 (5′-GAAAACCTGGGCATATCAATTTGCGGAGAA-3′) and R5764 (5′-AGCACCTTGGCCCAATAAACACTTGTCAGG-3′). .. The amplified product was purified and cloned into the pGemT Easy vector (Promega, Madison, WI) and transformed into E. coli NEB 10-beta cells.

    Article Title: Wnt/β-catenin signalling is necessary for gut differentiation in a marine annelid, Platynereis dumerilii
    Article Snippet: .. The C-clamp (−) isoform, X7, of Pdu - Tcf cDNA was amplified by polymerase chain reaction using Long PCR Enzyme Mix (Thermo Fisher Scientific#K0182), forward primer TcfPlatyRT-1 (5′-GGGAGATTTTCATGGCGGATTCA-3′) and the reverse primer TcfPlatyRT-4 (5′-CAGTTAGATCAAGCAGAGGTCAGAAGTAATACC-3′) on mixed stage Platynereis cDNA as a template. cDNA was synthesized using SuperScript™ II RT (Invitrogen 18064014) and random hexamer primers (Invitrogen 48190-011) following the manufacturer’s protocol from mRNA isolated with TRIzol™ Reagent (Invitrogen 15596026) according to manufacturer’s instructions. .. Conditions for PCR were as follows: initial denaturation 95 °C/2 min followed by 30 cycles of 95 °C/20 s denaturation, 61 °C/30 s annealing and at 68 °C/3-min extension, closed by an additional extension period of 10 min.

    Real-time Polymerase Chain Reaction:

    Article Title: Radical-scavenging and Pro-/anti-inflammatory Activity of Tetracycline and Related Phenolic Compounds With or Without Visible Light Irradiation
    Article Snippet: .. TaqMan probes/primers for Cox2 , Nos2 , Tnf α , Ho-1 and 18s rRNA and the PCR enzyme mix for real-time PCR were purchased from Life Technologies Japan. .. Real-time PCR quantification was performed in triplicate using the GeneAmp Sequence Detection System 5700 software (Life Technologies, Japan) in accordance with the manufacturer’s instructions.

    Concentration Assay:

    Article Title: Separation of 1-23-kb complementary DNA strands by urea-agarose gel electrophoresis
    Article Snippet: .. PCR amplification of S. cerevisae rDNA segments PCR was performed using 1.25 U of the Long PCR Enzyme mix (Fermentas) in 50 μl of 1× buffer supplemented with 1.5 mM MgCl2 , containing, 20 pmol of each primer (Integrated DNA Technologies, Coralville, IA, USA), the dNTPs (Promega) at 0.25 mM concentration and 300 ng S. cerevisae genomic DNA prepared as described earlier ( ). ..

    Random Hexamer Labeling:

    Article Title: Wnt/β-catenin signalling is necessary for gut differentiation in a marine annelid, Platynereis dumerilii
    Article Snippet: .. The C-clamp (−) isoform, X7, of Pdu - Tcf cDNA was amplified by polymerase chain reaction using Long PCR Enzyme Mix (Thermo Fisher Scientific#K0182), forward primer TcfPlatyRT-1 (5′-GGGAGATTTTCATGGCGGATTCA-3′) and the reverse primer TcfPlatyRT-4 (5′-CAGTTAGATCAAGCAGAGGTCAGAAGTAATACC-3′) on mixed stage Platynereis cDNA as a template. cDNA was synthesized using SuperScript™ II RT (Invitrogen 18064014) and random hexamer primers (Invitrogen 48190-011) following the manufacturer’s protocol from mRNA isolated with TRIzol™ Reagent (Invitrogen 15596026) according to manufacturer’s instructions. .. Conditions for PCR were as follows: initial denaturation 95 °C/2 min followed by 30 cycles of 95 °C/20 s denaturation, 61 °C/30 s annealing and at 68 °C/3-min extension, closed by an additional extension period of 10 min.

    other:

    Article Title: CircFOXK2 Promotes Tumor Growth and Metastasis of Pancreatic Ductal Adenocarcinoma via Complexing with RNA Binding Proteins and Sponging MiR-942
    Article Snippet: 1 μg template DNA was mixed with 1 μL of ATP solution, 1 μL of CTP solution, 1 μL of GTP solution, 0.9 μL of UTP solution, 0.15 μL of biotin-UTP solution (Epicentre), 1 μL of enzyme mixture and RNase-free water to 10 μL.

    Expressing:

    Article Title: A threshold level of NFATc1 activity facilitates thymocyte differentiation and opposes notch-driven leukaemia development
    Article Snippet: .. RT–PCRs to check the expression of six Nfatc1 isoform were performed using the Fermentas Long distance PCR Kit (Cat. # K0181). ..

    IA:

    Article Title: Separation of 1-23-kb complementary DNA strands by urea-agarose gel electrophoresis
    Article Snippet: .. PCR amplification of S. cerevisae rDNA segments PCR was performed using 1.25 U of the Long PCR Enzyme mix (Fermentas) in 50 μl of 1× buffer supplemented with 1.5 mM MgCl2 , containing, 20 pmol of each primer (Integrated DNA Technologies, Coralville, IA, USA), the dNTPs (Promega) at 0.25 mM concentration and 300 ng S. cerevisae genomic DNA prepared as described earlier ( ). ..

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Thermo Fisher elongase enzyme mix
    Elongase Enzyme Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elongase enzyme mix/product/Thermo Fisher
    Average 94 stars, based on 100 article reviews
    Price from $9.99 to $1999.99
    elongase enzyme mix - by Bioz Stars, 2021-02
    94/100 stars
      Buy from Supplier

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