elisa  (R&D Systems)

 
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    Name:
    Deconjugating Enzyme FRET K48 linked Substrate Kit Protein
    Description:
    Contains 10 ug each of rhDi Ub K48 linked FRET TAMRA Pos1 rhDi Ub K48 linked FRET EDANS Pos1 rhDi Ub K48 linked FRET TAMRA Pos2 rhDi Ub K48 linked FRET EDANS Pos2 rhDi Ub K48 linked FRET TAMRA Pos3 rhDi Ub K48 linked FRET EDANS Pos3
    Catalog Number:
    K-S12
    Price:
    395
    Category:
    Proteins and Enzymes
    Conjugate:
    Unconjugated
    Size:
    1 Kit
    Buy from Supplier


    Structured Review

    R&D Systems elisa
    Contains 10 ug each of rhDi Ub K48 linked FRET TAMRA Pos1 rhDi Ub K48 linked FRET EDANS Pos1 rhDi Ub K48 linked FRET TAMRA Pos2 rhDi Ub K48 linked FRET EDANS Pos2 rhDi Ub K48 linked FRET TAMRA Pos3 rhDi Ub K48 linked FRET EDANS Pos3
    https://www.bioz.com/result/elisa/product/R&D Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa - by Bioz Stars, 2021-04
    86/100 stars

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    Purification:

    Article Title: Insights into the Porcine Reproductive and Respiratory Syndrome Virus Viral Ovarian Tumor Domain Protease Specificity for Ubiquitin and Interferon Stimulated Gene Product 15
    Article Snippet: To assess purified wild-type and mutant PRRSV vOTUs activity, fluorescence assays were performed as described previously. .. Briefly, purified PRRSV vOTUs were tested against Ub, ISG15, and Z-RLRGG peptide conjugated to 7-amino-4-methyl-coumarin (AMC) and di-Ub fluorescence resonance energy transfer (FRET) linkage substrates K11, K48, and K63 (Boston Biochem, MA). ..

    Article Title: Structural Basis for the Ubiquitin-Linkage Specificity and deISGylating Activity of SARS-CoV Papain-Like Protease
    Article Snippet: .. Ubiquitin chain cleavage experiments Proteolytic cleavage of homogeneous, K48-linked or K63-linked penta-ubiquitin (Boston Biochem) was carried out under the following conditions: 0.07 µg of purified PLpro (23 nM) was incubated with 50 µg of K48-Ub5 or K63-Ub5 at 25°C in an 85 µL volume containing 50 mM HEPES, pH 7.5, 0.1 mg/mL BSA, 100 mM NaCl, and 2 mM DTT. ..

    Fluorescence:

    Article Title: Insights into the Porcine Reproductive and Respiratory Syndrome Virus Viral Ovarian Tumor Domain Protease Specificity for Ubiquitin and Interferon Stimulated Gene Product 15
    Article Snippet: To assess purified wild-type and mutant PRRSV vOTUs activity, fluorescence assays were performed as described previously. .. Briefly, purified PRRSV vOTUs were tested against Ub, ISG15, and Z-RLRGG peptide conjugated to 7-amino-4-methyl-coumarin (AMC) and di-Ub fluorescence resonance energy transfer (FRET) linkage substrates K11, K48, and K63 (Boston Biochem, MA). ..

    Article Title: Diversity of Ubiquitin and ISG15 Specificity among Nairoviruses' Viral Ovarian Tumor Domain Proteases
    Article Snippet: .. The vOTUs from CCHFV, DUGV, ERVEV, and TYMV turnover rates for poly-Ub Förster resonance energy transfer (FRET) linkage substrates K11, K48, and K63 (Boston Biochem, MA) at 1 μM were determined by monitoring the increase in fluorescence (excitation λ, 544 nm; emission, 572 nm) resulting by the separation of a FRET TAMRA/QXL pair. ..

    Förster Resonance Energy Transfer:

    Article Title: Insights into the Porcine Reproductive and Respiratory Syndrome Virus Viral Ovarian Tumor Domain Protease Specificity for Ubiquitin and Interferon Stimulated Gene Product 15
    Article Snippet: To assess purified wild-type and mutant PRRSV vOTUs activity, fluorescence assays were performed as described previously. .. Briefly, purified PRRSV vOTUs were tested against Ub, ISG15, and Z-RLRGG peptide conjugated to 7-amino-4-methyl-coumarin (AMC) and di-Ub fluorescence resonance energy transfer (FRET) linkage substrates K11, K48, and K63 (Boston Biochem, MA). ..

    Article Title: Diversity of Ubiquitin and ISG15 Specificity among Nairoviruses' Viral Ovarian Tumor Domain Proteases
    Article Snippet: .. The vOTUs from CCHFV, DUGV, ERVEV, and TYMV turnover rates for poly-Ub Förster resonance energy transfer (FRET) linkage substrates K11, K48, and K63 (Boston Biochem, MA) at 1 μM were determined by monitoring the increase in fluorescence (excitation λ, 544 nm; emission, 572 nm) resulting by the separation of a FRET TAMRA/QXL pair. ..

    Incubation:

    Article Title: Structural Basis for the Ubiquitin-Linkage Specificity and deISGylating Activity of SARS-CoV Papain-Like Protease
    Article Snippet: .. Ubiquitin chain cleavage experiments Proteolytic cleavage of homogeneous, K48-linked or K63-linked penta-ubiquitin (Boston Biochem) was carried out under the following conditions: 0.07 µg of purified PLpro (23 nM) was incubated with 50 µg of K48-Ub5 or K63-Ub5 at 25°C in an 85 µL volume containing 50 mM HEPES, pH 7.5, 0.1 mg/mL BSA, 100 mM NaCl, and 2 mM DTT. ..

    Article Title: Polyubiquitin binding to ABIN1 is required to prevent autoimmunity
    Article Snippet: Human NEMO and NEMO[D311N] were expressed as GST fusions and purified on glutathione-Sepharose. .. 8 µg of these proteins were immobilized individually on a 10-µl packed volume of glutathione-Sepharose and incubated for 1 h at 21°C with 2 µg of K48-linked, K63-linked (Boston Biochem), or linear ubiquitin oligomers (Enzo Life Sciences) in 300 µl 25 mM HEPES, pH 7.5, 1 mM EGTA, 0.5% (vol/vol) Triton X-100, and 2 mM MgCl2 (Buffer A) plus 150 mM NaCl. ..

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    R&D Systems tgf β1 elisa kit
    Toxoplasma WH3Δ rop16 strain infection induced impairments of T regulatory cells (Tregs). Tregs cell population in the spleens (A) and placentas (B) were detected by flow cytometry. Tregs number was significantly decreased in WH3Δ rop16 infected group compared to control group. The relative mRNA expressions interleukin (IL)-10 and transforming growth factor beta 1 <t>(TGF-β1)</t> in the spleens (C) and placentas (D) were markedly reduced in WH3Δ rop16 group. The levels of IL-10 and TGF-β1 in the spleens (pg/ml) (E) and placentas (pg/mg) (F) were significantly decreased in the group of WH3Δ rop16 (* p
    Tgf β1 Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tgf β1 elisa kit/product/R&D Systems
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tgf β1 elisa kit - by Bioz Stars, 2021-04
    97/100 stars
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    99
    R&D Systems elisa kits
    Comparison of top-performing detection and capture antibodies. (A) Four detection antibodies from commercial IL-6 kits were screened and compared for their ability to recognize human recombinant IL-6. Thermo-Pierce 5IL6 was used as a capture antibody. Invitrogen detection antibody (505E23C7) was selected as the detection antibody for future screenings. (B) The three top-performing capture monoclonal anti-IL-6 antibodies (Thermo-Pierce 5IL6, Ebiosciences 16YOR5/66 and Epitomics EBI-R14-19) were compared in a single analysis. The detection antibody was Invitrogen <t>ELISA</t> detection antibody 505E23C7. IL-6, interleukin 6; hIL6, human interleukin 6; ELISA, enzyme-linked immunosorbent assay; OD, optical density; det Ab, detection antibody, R D det mAb, Thermo-Pierce det mAb, <t>PeproTech</t> det mAb, Invitrogen det mAb, Thermo-Pierce 5IL6, Ebiosciences 16YOR5/66, Epitomics EBI-R14-19
    Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kits/product/R&D Systems
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa kits - by Bioz Stars, 2021-04
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    93
    R&D Systems il 5 elisa duo set kit
    Dual engagement of EP2 and EP4 receptors is essential for the PGE 2 -induced reduction of ILC2 cytokine production. Sorted and expanded tonsillar ILC2s were stimulated as indicated for 24 hours. Additionally, the EP2 receptor antagonist PF-04418948 (PF) and the EP4 receptor antagonist ONO AE3-208 (ONO) were added separately or together in different concentrations (100 nmol/L, 300 nmol/L, 1 μmol/L, and 3 μmol/L) 20 minutes before PGE 2 . A and B, Concentrations of released <t>IL-5</t> ( Fig E4 , A ) and IL-13 ( Fig E4 , B ) in ILC2 supernatants were determined by using <t>ELISA</t> and shown as means ± SEM (n = 4). C and D, Sorted and expanded tonsillar ILC2s were treated with the EP2 receptor agonist butaprost (But) and the EP4 receptor agonist L-902,688 (L9) separately or together in different concentrations (10 nmol/L, 30 nmol/L, 100 nmol/L, 300 nmol/L, and 1 μmol/L) 10 minutes before the stimulatory cytokines. Concentrations of released IL-5 ( Fig E4 , C ) and IL-13 ( Fig E4 , D ) in ILC2 supernatants were determined by using ELISA and are shown as means ± SEMs (n = 4; compared with IL treatment). * P
    Il 5 Elisa Duo Set Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 5 elisa duo set kit/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 5 elisa duo set kit - by Bioz Stars, 2021-04
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    Image Search Results


    Toxoplasma WH3Δ rop16 strain infection induced impairments of T regulatory cells (Tregs). Tregs cell population in the spleens (A) and placentas (B) were detected by flow cytometry. Tregs number was significantly decreased in WH3Δ rop16 infected group compared to control group. The relative mRNA expressions interleukin (IL)-10 and transforming growth factor beta 1 (TGF-β1) in the spleens (C) and placentas (D) were markedly reduced in WH3Δ rop16 group. The levels of IL-10 and TGF-β1 in the spleens (pg/ml) (E) and placentas (pg/mg) (F) were significantly decreased in the group of WH3Δ rop16 (* p

    Journal: Frontiers in Immunology

    Article Title: Toxoplasma Chinese 1 Strain of WH3Δrop16I/III/gra15II Genetic Background Contributes to Abnormal Pregnant Outcomes in Murine Model

    doi: 10.3389/fimmu.2018.01222

    Figure Lengend Snippet: Toxoplasma WH3Δ rop16 strain infection induced impairments of T regulatory cells (Tregs). Tregs cell population in the spleens (A) and placentas (B) were detected by flow cytometry. Tregs number was significantly decreased in WH3Δ rop16 infected group compared to control group. The relative mRNA expressions interleukin (IL)-10 and transforming growth factor beta 1 (TGF-β1) in the spleens (C) and placentas (D) were markedly reduced in WH3Δ rop16 group. The levels of IL-10 and TGF-β1 in the spleens (pg/ml) (E) and placentas (pg/mg) (F) were significantly decreased in the group of WH3Δ rop16 (* p

    Article Snippet: IFN-γ, IL-12, IL-10, and TGF-β1 ELISA kit was obtained from R & D Systems (Minneapolis, MN, USA).

    Techniques: Infection, Flow Cytometry, Cytometry

    Comparison of top-performing detection and capture antibodies. (A) Four detection antibodies from commercial IL-6 kits were screened and compared for their ability to recognize human recombinant IL-6. Thermo-Pierce 5IL6 was used as a capture antibody. Invitrogen detection antibody (505E23C7) was selected as the detection antibody for future screenings. (B) The three top-performing capture monoclonal anti-IL-6 antibodies (Thermo-Pierce 5IL6, Ebiosciences 16YOR5/66 and Epitomics EBI-R14-19) were compared in a single analysis. The detection antibody was Invitrogen ELISA detection antibody 505E23C7. IL-6, interleukin 6; hIL6, human interleukin 6; ELISA, enzyme-linked immunosorbent assay; OD, optical density; det Ab, detection antibody, R D det mAb, Thermo-Pierce det mAb, PeproTech det mAb, Invitrogen det mAb, Thermo-Pierce 5IL6, Ebiosciences 16YOR5/66, Epitomics EBI-R14-19

    Journal: British Journal of Clinical Pharmacology

    Article Title: Development and validation of panoptic Meso scale discovery assay to quantify total systemic interleukin-6

    doi: 10.1111/bcp.12652

    Figure Lengend Snippet: Comparison of top-performing detection and capture antibodies. (A) Four detection antibodies from commercial IL-6 kits were screened and compared for their ability to recognize human recombinant IL-6. Thermo-Pierce 5IL6 was used as a capture antibody. Invitrogen detection antibody (505E23C7) was selected as the detection antibody for future screenings. (B) The three top-performing capture monoclonal anti-IL-6 antibodies (Thermo-Pierce 5IL6, Ebiosciences 16YOR5/66 and Epitomics EBI-R14-19) were compared in a single analysis. The detection antibody was Invitrogen ELISA detection antibody 505E23C7. IL-6, interleukin 6; hIL6, human interleukin 6; ELISA, enzyme-linked immunosorbent assay; OD, optical density; det Ab, detection antibody, R D det mAb, Thermo-Pierce det mAb, PeproTech det mAb, Invitrogen det mAb, Thermo-Pierce 5IL6, Ebiosciences 16YOR5/66, Epitomics EBI-R14-19

    Article Snippet: To identify the optimal anti-IL-6 detection antibody in detecting recombinant human IL-6 spiked into human serum, four anti-IL-6 detection antibodies from commercially available ELISA kits (R & D Systems, Thermo Pierce, PeproTech and Invitrogen) were compared in standard ELISA format using Thermo-Pierce anti-IL-6 antibody (5IL6) as a capture antibody (Figure ).

    Techniques: Recombinant, Enzyme-linked Immunosorbent Assay

    TNF-α induced by TSP50 is crucial for affecting macrophage activities. (A-B) TSP50-o/e cells were transfected with the indicated shRNA plasmids. After 24h, the mRNA level of TNF-α (A) or cytokines (B) in these cells were analyzed by real-time PCR. (C) dTHP-1 cells or mouse peritoneal macrophages were cultured with CM from TNF-α knock-down TSP50-o/e cells or control cells for 24h. The mRNA expression of cytokines and macrophage phenotypic markers were determined by real-time PCR. (D) Mouse peritoneal macrophages were cultured with CM from TNF-α knock-down TSP50-o/e cells or control cells for 24h. CM from macrophages was collected and subjected to ELISA to detect the secretion of cytokines. (E) Mouse macrophages were cultured with CM from TNF-α knock-down TSP50-o/e cells or control cells for 24h. The macrophages were collected and lysed, and the protein level of macrophage phenotype markers were analyzed by western blotting. (F) Phagocytosis capacities of dTHP-1 cells (up) or mouse peritoneal macrophages (down) were measured after treatment with CM from TNF-α knock-down TSP50-o/e cells or control cells. GAPDH was used as the internal control to check the efficiency of cDNA synthesis and PCR amplification. Data are shown as mean ± SD of three replicates in three independent experiments.* p

    Journal: PLoS ONE

    Article Title: Protumoral TSP50 Regulates Macrophage Activities and Polarization via Production of TNF-α and IL-1β, and Activation of the NF-κB Signaling Pathway

    doi: 10.1371/journal.pone.0145095

    Figure Lengend Snippet: TNF-α induced by TSP50 is crucial for affecting macrophage activities. (A-B) TSP50-o/e cells were transfected with the indicated shRNA plasmids. After 24h, the mRNA level of TNF-α (A) or cytokines (B) in these cells were analyzed by real-time PCR. (C) dTHP-1 cells or mouse peritoneal macrophages were cultured with CM from TNF-α knock-down TSP50-o/e cells or control cells for 24h. The mRNA expression of cytokines and macrophage phenotypic markers were determined by real-time PCR. (D) Mouse peritoneal macrophages were cultured with CM from TNF-α knock-down TSP50-o/e cells or control cells for 24h. CM from macrophages was collected and subjected to ELISA to detect the secretion of cytokines. (E) Mouse macrophages were cultured with CM from TNF-α knock-down TSP50-o/e cells or control cells for 24h. The macrophages were collected and lysed, and the protein level of macrophage phenotype markers were analyzed by western blotting. (F) Phagocytosis capacities of dTHP-1 cells (up) or mouse peritoneal macrophages (down) were measured after treatment with CM from TNF-α knock-down TSP50-o/e cells or control cells. GAPDH was used as the internal control to check the efficiency of cDNA synthesis and PCR amplification. Data are shown as mean ± SD of three replicates in three independent experiments.* p

    Article Snippet: Then the levels of human IL-1β, IL-10, IL-12, murine IL-1β, IL-6, IL-10, IL-12, TNF-α or TGF-β in the supernatants were determined using commercially available ELISA kits (R & D Systems, Minneapolis, MN, USA), following the protocols supplied by the manufacturers.

    Techniques: Transfection, shRNA, Real-time Polymerase Chain Reaction, Cell Culture, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Polymerase Chain Reaction, Amplification

    CM of TSP50-o/e cells regulates of macrophages activities via NF-κB pathway. (A) Macrophages were treated with CM from TSP50-o/e cells or control cells for 15min, 30min or 60min. The activation of NF-κB pathway in dTHP-1 cells was then analyzed by western blotting. (B) Mouse macrophages were treated with CM from TNF-α knock-down TSP50-o/e cells for 30min. The activation of NF-κB pathway in dTHP-1 cells was then analyzed by western blotting. (C) dTHP-1 cells were pretreated with 25μg/mL of NF-κB inhibitor PDTC for 30min and then the culture medium were replaced with fresh medium containing 30% of CM from TSP50-o/e cells or control cells. After 30-min incubation, the activation of NF-κB pathway was analyzed by western blotting. (D) dTHP-1 were treated with PDTC and CM from TSP50-o/e cells for 24 hours. The CMs were collected and subjected to ELISA to detect the production of IL-1β. (E) dTHP-1 cells and mouse peritoneal macrophages were incubated in culture medium containing PDTC and CM of TSP50-o/e cells or control cells for 24 hours. Real-time PCR was used to determine the mRNA level of macrophage phenotypic markers. (F) Phagocytic activities of mouse peritoneal macrophages were determined following co-treatment with PDTC and CM from TSP50-o/e cells for 24 hours. GAPDH was used as the internal control to check the efficiency of cDNA synthesis and PCR amplification. Data are shown as mean ± SD of three independent experiments. * p

    Journal: PLoS ONE

    Article Title: Protumoral TSP50 Regulates Macrophage Activities and Polarization via Production of TNF-α and IL-1β, and Activation of the NF-κB Signaling Pathway

    doi: 10.1371/journal.pone.0145095

    Figure Lengend Snippet: CM of TSP50-o/e cells regulates of macrophages activities via NF-κB pathway. (A) Macrophages were treated with CM from TSP50-o/e cells or control cells for 15min, 30min or 60min. The activation of NF-κB pathway in dTHP-1 cells was then analyzed by western blotting. (B) Mouse macrophages were treated with CM from TNF-α knock-down TSP50-o/e cells for 30min. The activation of NF-κB pathway in dTHP-1 cells was then analyzed by western blotting. (C) dTHP-1 cells were pretreated with 25μg/mL of NF-κB inhibitor PDTC for 30min and then the culture medium were replaced with fresh medium containing 30% of CM from TSP50-o/e cells or control cells. After 30-min incubation, the activation of NF-κB pathway was analyzed by western blotting. (D) dTHP-1 were treated with PDTC and CM from TSP50-o/e cells for 24 hours. The CMs were collected and subjected to ELISA to detect the production of IL-1β. (E) dTHP-1 cells and mouse peritoneal macrophages were incubated in culture medium containing PDTC and CM of TSP50-o/e cells or control cells for 24 hours. Real-time PCR was used to determine the mRNA level of macrophage phenotypic markers. (F) Phagocytic activities of mouse peritoneal macrophages were determined following co-treatment with PDTC and CM from TSP50-o/e cells for 24 hours. GAPDH was used as the internal control to check the efficiency of cDNA synthesis and PCR amplification. Data are shown as mean ± SD of three independent experiments. * p

    Article Snippet: Then the levels of human IL-1β, IL-10, IL-12, murine IL-1β, IL-6, IL-10, IL-12, TNF-α or TGF-β in the supernatants were determined using commercially available ELISA kits (R & D Systems, Minneapolis, MN, USA), following the protocols supplied by the manufacturers.

    Techniques: Activation Assay, Western Blot, Incubation, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Amplification

    Dual engagement of EP2 and EP4 receptors is essential for the PGE 2 -induced reduction of ILC2 cytokine production. Sorted and expanded tonsillar ILC2s were stimulated as indicated for 24 hours. Additionally, the EP2 receptor antagonist PF-04418948 (PF) and the EP4 receptor antagonist ONO AE3-208 (ONO) were added separately or together in different concentrations (100 nmol/L, 300 nmol/L, 1 μmol/L, and 3 μmol/L) 20 minutes before PGE 2 . A and B, Concentrations of released IL-5 ( Fig E4 , A ) and IL-13 ( Fig E4 , B ) in ILC2 supernatants were determined by using ELISA and shown as means ± SEM (n = 4). C and D, Sorted and expanded tonsillar ILC2s were treated with the EP2 receptor agonist butaprost (But) and the EP4 receptor agonist L-902,688 (L9) separately or together in different concentrations (10 nmol/L, 30 nmol/L, 100 nmol/L, 300 nmol/L, and 1 μmol/L) 10 minutes before the stimulatory cytokines. Concentrations of released IL-5 ( Fig E4 , C ) and IL-13 ( Fig E4 , D ) in ILC2 supernatants were determined by using ELISA and are shown as means ± SEMs (n = 4; compared with IL treatment). * P

    Journal: The Journal of Allergy and Clinical Immunology

    Article Title: Prostaglandin E2 suppresses human group 2 innate lymphoid cell function

    doi: 10.1016/j.jaci.2017.09.050

    Figure Lengend Snippet: Dual engagement of EP2 and EP4 receptors is essential for the PGE 2 -induced reduction of ILC2 cytokine production. Sorted and expanded tonsillar ILC2s were stimulated as indicated for 24 hours. Additionally, the EP2 receptor antagonist PF-04418948 (PF) and the EP4 receptor antagonist ONO AE3-208 (ONO) were added separately or together in different concentrations (100 nmol/L, 300 nmol/L, 1 μmol/L, and 3 μmol/L) 20 minutes before PGE 2 . A and B, Concentrations of released IL-5 ( Fig E4 , A ) and IL-13 ( Fig E4 , B ) in ILC2 supernatants were determined by using ELISA and shown as means ± SEM (n = 4). C and D, Sorted and expanded tonsillar ILC2s were treated with the EP2 receptor agonist butaprost (But) and the EP4 receptor agonist L-902,688 (L9) separately or together in different concentrations (10 nmol/L, 30 nmol/L, 100 nmol/L, 300 nmol/L, and 1 μmol/L) 10 minutes before the stimulatory cytokines. Concentrations of released IL-5 ( Fig E4 , C ) and IL-13 ( Fig E4 , D ) in ILC2 supernatants were determined by using ELISA and are shown as means ± SEMs (n = 4; compared with IL treatment). * P

    Article Snippet: ELISA Collected cell supernatants were analyzed with the IL-5 ELISA Duo-Set Kit (R & D Systems, Minneapolis, Minn) and the IL-13 ELISA Kit (Sanquin, Amsterdam, The Netherlands).

    Techniques: Enzyme-linked Immunosorbent Assay

    PGE 2 reduces GATA-3 upregulation. ILC2s were stimulated as indicated for 72 hours. A and B, Concentrations of IL-5 (Fig 3, A ) and IL-13 (Fig 3, B ) in ILC2 supernatants were determined by means of ELISA, and data are shown as individual concentrations (n = 10). *** P

    Journal: The Journal of Allergy and Clinical Immunology

    Article Title: Prostaglandin E2 suppresses human group 2 innate lymphoid cell function

    doi: 10.1016/j.jaci.2017.09.050

    Figure Lengend Snippet: PGE 2 reduces GATA-3 upregulation. ILC2s were stimulated as indicated for 72 hours. A and B, Concentrations of IL-5 (Fig 3, A ) and IL-13 (Fig 3, B ) in ILC2 supernatants were determined by means of ELISA, and data are shown as individual concentrations (n = 10). *** P

    Article Snippet: ELISA Collected cell supernatants were analyzed with the IL-5 ELISA Duo-Set Kit (R & D Systems, Minneapolis, Minn) and the IL-13 ELISA Kit (Sanquin, Amsterdam, The Netherlands).

    Techniques: Enzyme-linked Immunosorbent Assay

    PGE 2 concentration-dependently inhibits cytokine production of ILC2s. A and B, Sorted and expanded tonsillar ILC2s were stimulated with a combination of cytokines (IL-33, TSLP, and IL-25 plus IL-2) for 24 hours. PGE 2 was added in different concentrations (3, 10, 30, 100, and 300 nmol/L) 10 minutes before the cytokines. Concentrations of IL-5 (Fig E3, A ) and IL-13 (Fig E3, B ) in ILC2 supernatants were determined by means of ELISA, and data are shown as mean ± SEM concentrations (n = 4). * P

    Journal: The Journal of Allergy and Clinical Immunology

    Article Title: Prostaglandin E2 suppresses human group 2 innate lymphoid cell function

    doi: 10.1016/j.jaci.2017.09.050

    Figure Lengend Snippet: PGE 2 concentration-dependently inhibits cytokine production of ILC2s. A and B, Sorted and expanded tonsillar ILC2s were stimulated with a combination of cytokines (IL-33, TSLP, and IL-25 plus IL-2) for 24 hours. PGE 2 was added in different concentrations (3, 10, 30, 100, and 300 nmol/L) 10 minutes before the cytokines. Concentrations of IL-5 (Fig E3, A ) and IL-13 (Fig E3, B ) in ILC2 supernatants were determined by means of ELISA, and data are shown as mean ± SEM concentrations (n = 4). * P

    Article Snippet: ELISA Collected cell supernatants were analyzed with the IL-5 ELISA Duo-Set Kit (R & D Systems, Minneapolis, Minn) and the IL-13 ELISA Kit (Sanquin, Amsterdam, The Netherlands).

    Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

    PGE 2 reduces IL-5 and IL-13 secretion from fresh blood and tonsillar ILC2s. Freshly sorted human blood (A-E) and tonsillar ( F and G ) ILC2s were incubated as indicated for 5 days. Concentrations of released IL-5 from blood ILC2s (Fig 4, A ) and tonsillar ILC2s (Fig 4, F ) and IL-13 from blood ILC2s (Fig 4, B ) and tonsillar ILC2s (Fig 4, G ) were measured by means of ELISA, and graphs show individual concentrations (n = 6 for blood ILC2s and n = 4 for tonsillar ILC2s). * P

    Journal: The Journal of Allergy and Clinical Immunology

    Article Title: Prostaglandin E2 suppresses human group 2 innate lymphoid cell function

    doi: 10.1016/j.jaci.2017.09.050

    Figure Lengend Snippet: PGE 2 reduces IL-5 and IL-13 secretion from fresh blood and tonsillar ILC2s. Freshly sorted human blood (A-E) and tonsillar ( F and G ) ILC2s were incubated as indicated for 5 days. Concentrations of released IL-5 from blood ILC2s (Fig 4, A ) and tonsillar ILC2s (Fig 4, F ) and IL-13 from blood ILC2s (Fig 4, B ) and tonsillar ILC2s (Fig 4, G ) were measured by means of ELISA, and graphs show individual concentrations (n = 6 for blood ILC2s and n = 4 for tonsillar ILC2s). * P

    Article Snippet: ELISA Collected cell supernatants were analyzed with the IL-5 ELISA Duo-Set Kit (R & D Systems, Minneapolis, Minn) and the IL-13 ELISA Kit (Sanquin, Amsterdam, The Netherlands).

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay

    PGE 2 suppresses IL-5 and IL-13 production of human tonsillar ILC2s. A, Gating strategy for sort-purifying human tonsillar ILC2s. Sorted and expanded tonsillar ILC2s in the presence of IL-2 (10 U/mL) were stimulated with IL-33, IL-25, and TSLP (50 ng/mL each), shortened as IL, or left nonstimulated for 24 hours. PGE 2 (30 nmol/L) was added 10 minutes before the cytokines. B-D, Concentrations of released IL-5 (Fig 1, B and D ) and IL-13 (Fig 1, C and D ) in ILC2 supernatants were assessed by means of ELISA, and graphs show individual concentrations (n = 9-10). **** P

    Journal: The Journal of Allergy and Clinical Immunology

    Article Title: Prostaglandin E2 suppresses human group 2 innate lymphoid cell function

    doi: 10.1016/j.jaci.2017.09.050

    Figure Lengend Snippet: PGE 2 suppresses IL-5 and IL-13 production of human tonsillar ILC2s. A, Gating strategy for sort-purifying human tonsillar ILC2s. Sorted and expanded tonsillar ILC2s in the presence of IL-2 (10 U/mL) were stimulated with IL-33, IL-25, and TSLP (50 ng/mL each), shortened as IL, or left nonstimulated for 24 hours. PGE 2 (30 nmol/L) was added 10 minutes before the cytokines. B-D, Concentrations of released IL-5 (Fig 1, B and D ) and IL-13 (Fig 1, C and D ) in ILC2 supernatants were assessed by means of ELISA, and graphs show individual concentrations (n = 9-10). **** P

    Article Snippet: ELISA Collected cell supernatants were analyzed with the IL-5 ELISA Duo-Set Kit (R & D Systems, Minneapolis, Minn) and the IL-13 ELISA Kit (Sanquin, Amsterdam, The Netherlands).

    Techniques: Enzyme-linked Immunosorbent Assay

    PGE 2 reduces IL-5 and IL-13 production in ILC2s through activation of EP2 and EP4 receptors. Sorted and expanded tonsillar ILC2s were stimulated as indicated for 24 hours. The EP2 receptor antagonist PF-04418948 ( PF ; 1 μmol/L) and the EP4 receptor antagonist ONO AE3-208 ( ONO ; 1 μmol/L) were added separately or together 20 minutes before PGE 2 . A and B, Concentrations of released IL-5 (Fig 6, A ) and IL-13 (Fig 6, B ) in ILC2 supernatants were determined by means of ELISA. Bar graphs show means + SEMs. (n = 6-7). * P

    Journal: The Journal of Allergy and Clinical Immunology

    Article Title: Prostaglandin E2 suppresses human group 2 innate lymphoid cell function

    doi: 10.1016/j.jaci.2017.09.050

    Figure Lengend Snippet: PGE 2 reduces IL-5 and IL-13 production in ILC2s through activation of EP2 and EP4 receptors. Sorted and expanded tonsillar ILC2s were stimulated as indicated for 24 hours. The EP2 receptor antagonist PF-04418948 ( PF ; 1 μmol/L) and the EP4 receptor antagonist ONO AE3-208 ( ONO ; 1 μmol/L) were added separately or together 20 minutes before PGE 2 . A and B, Concentrations of released IL-5 (Fig 6, A ) and IL-13 (Fig 6, B ) in ILC2 supernatants were determined by means of ELISA. Bar graphs show means + SEMs. (n = 6-7). * P

    Article Snippet: ELISA Collected cell supernatants were analyzed with the IL-5 ELISA Duo-Set Kit (R & D Systems, Minneapolis, Minn) and the IL-13 ELISA Kit (Sanquin, Amsterdam, The Netherlands).

    Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay