elisa kits detecting mpo  (Hycult Biotech)

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: FGL2–MCOLN3-Autophagy Axis–Triggered Neutrophil Extracellular Traps Exacerbate Liver Injury in Fulminant Viral Hepatitis

doi: 10.1016/j.jcmgh.2022.07.014

Figure Lengend Snippet: Reagents Used in This Study

Article Snippet: Human MPO ELISA kit , BioLegend (San Diego, CA) , , 440007.

Techniques: Isolation, Enzyme-linked Immunosorbent Assay, Magnetic Beads, Polyacrylamide Gel Electrophoresis, Lysis, Immunoprecipitation, SYBR Green Assay, Real-time Polymerase Chain Reaction

Journal: eBioMedicine

Article Title: Dysregulation of the kallikrein-kinin system in bronchoalveolar lavage fluid of patients with severe COVID-19

doi: 10.1016/j.ebiom.2022.104195

Figure Lengend Snippet: MPO-DNA complexes in bronchoalveolar lavage fluid from patients with and without COVID-19 . Graphs show individual dots representing patient data and bar representing median of each patient group. For graphical representation, data were transformed using the logarithmic transformation. Pairwise comparisons were made between patients with COVID-19 ( n = 21) and patients without COVID-19 ( n = 19). Median [range] and p -value are shown (Mann-Whitney U test).

Article Snippet: A standard curve was prepared by incubating a known quantity of an in-house-generated MPO-DNA mixture complex consisting of enzymatically inactive MPO standard (from the LEGEND MAX Human MPO ELISA kit, Biolegend), lambda-DNA (Invitrogen) and human native nucleosomes (Sigma).

Techniques: Transformation Assay, MANN-WHITNEY

Journal: Veterinary Sciences

Article Title: Investigation of Anti-Myeloperoxidase Antibodies in a Dog with Bilateral Necrotizing Scleritis

doi: 10.3390/vetsci2030259

Figure Lengend Snippet: Results of the anti-MPO ELISA. A commercial kit developed to detect anti-MPO antibodies in human sera, was used to test our samples. We followed the manufacturer’s instructions, but used an HRP anti-dog IgG secondary antibody rather than the anti-human secondary provided with the kit. Absorbance values were obtained using a multi-function plate reader. Using the “average + 2 or 3 standard deviations” cut-off, our patient was not positive for anti-MPO antibodies, but one of the control dogs was.

Article Snippet: Two months after prednisolone discontinuation, serum samples from this dog and 13 healthy dogs were analyzed using a commercial anti-human myeloperoxidase (MPO) detection ELISA kit (Diamedix Inc., Miami Lakes, FL, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Control