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Stratagene electrocompetent e coli xl1 blue mrf cells
Electrocompetent E Coli Xl1 Blue Mrf Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/electrocompetent e coli xl1 blue mrf cells/product/Stratagene
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
electrocompetent e coli xl1 blue mrf cells - by Bioz Stars, 2020-04
86/100 stars

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Electroporation:

Article Title: Identification of Mycobacterium avium Genes That Affect Invasion of the Intestinal Epithelium
Article Snippet: Paragraph title: Electroporation of M. smegmatis and E. coli . ... Cells were recovered with 1 ml 7H9 and incubated at 37°C with shaking for 2 h. Electrocompetent E. coli XL1-Blue MRF′ cells were either purchased from Stratagene or prepared and transformed as previously described ( ).

Amplification:

Article Title: The bcr1 DNA Repeat Element Is Specific to the Bacillus cereus Group and Exhibits Mobile Element Characteristics
Article Snippet: Paragraph title: PCR amplification and cloning of bcr1 . ... Furthermore, a plot of the conservation of each site was generated by CLUSTALX ( bcr1 PCR products were purified with the QIAquick PCR purification kit (QIAGEN GmbH, Hilden, Germany) and cloned into a pUC19 vector by using electrocompetent Escherichia coli XL1-Blue MRF′ cells (Stratagene, Cedar Falls, Tex.).

Clone Assay:

Article Title: The bcr1 DNA Repeat Element Is Specific to the Bacillus cereus Group and Exhibits Mobile Element Characteristics
Article Snippet: .. Furthermore, a plot of the conservation of each site was generated by CLUSTALX ( bcr1 PCR products were purified with the QIAquick PCR purification kit (QIAGEN GmbH, Hilden, Germany) and cloned into a pUC19 vector by using electrocompetent Escherichia coli XL1-Blue MRF′ cells (Stratagene, Cedar Falls, Tex.). .. Transformants were checked for the presence of the bcr1 insert by restriction digests and DNA sequencing.

Article Title: Cloning of a Phenol Oxidase Gene from Acremonium murorum and Its Expression in Aspergillus awamori
Article Snippet: cDNA was cloned as Eco RI/ Xho I fragments into plasmid pYES2.0 (Invitrogen). .. Aliquots of 2.5 μl were used to transform 50 μl of electrocompetent E. coli XL1-Blue MRF′ cells (Stratagene) (conditions, 1,700 V, 200 ω, and 25 μF).

Incubation:

Article Title: Identification of Mycobacterium avium Genes That Affect Invasion of the Intestinal Epithelium
Article Snippet: .. Cells were recovered with 1 ml 7H9 and incubated at 37°C with shaking for 2 h. Electrocompetent E. coli XL1-Blue MRF′ cells were either purchased from Stratagene or prepared and transformed as previously described ( ). ..

Purification:

Article Title: The bcr1 DNA Repeat Element Is Specific to the Bacillus cereus Group and Exhibits Mobile Element Characteristics
Article Snippet: .. Furthermore, a plot of the conservation of each site was generated by CLUSTALX ( bcr1 PCR products were purified with the QIAquick PCR purification kit (QIAGEN GmbH, Hilden, Germany) and cloned into a pUC19 vector by using electrocompetent Escherichia coli XL1-Blue MRF′ cells (Stratagene, Cedar Falls, Tex.). .. Transformants were checked for the presence of the bcr1 insert by restriction digests and DNA sequencing.

Polymerase Chain Reaction:

Article Title: The bcr1 DNA Repeat Element Is Specific to the Bacillus cereus Group and Exhibits Mobile Element Characteristics
Article Snippet: .. Furthermore, a plot of the conservation of each site was generated by CLUSTALX ( bcr1 PCR products were purified with the QIAquick PCR purification kit (QIAGEN GmbH, Hilden, Germany) and cloned into a pUC19 vector by using electrocompetent Escherichia coli XL1-Blue MRF′ cells (Stratagene, Cedar Falls, Tex.). .. Transformants were checked for the presence of the bcr1 insert by restriction digests and DNA sequencing.

Generated:

Article Title: The bcr1 DNA Repeat Element Is Specific to the Bacillus cereus Group and Exhibits Mobile Element Characteristics
Article Snippet: .. Furthermore, a plot of the conservation of each site was generated by CLUSTALX ( bcr1 PCR products were purified with the QIAquick PCR purification kit (QIAGEN GmbH, Hilden, Germany) and cloned into a pUC19 vector by using electrocompetent Escherichia coli XL1-Blue MRF′ cells (Stratagene, Cedar Falls, Tex.). .. Transformants were checked for the presence of the bcr1 insert by restriction digests and DNA sequencing.

DNA Sequencing:

Article Title: The bcr1 DNA Repeat Element Is Specific to the Bacillus cereus Group and Exhibits Mobile Element Characteristics
Article Snippet: Furthermore, a plot of the conservation of each site was generated by CLUSTALX ( bcr1 PCR products were purified with the QIAquick PCR purification kit (QIAGEN GmbH, Hilden, Germany) and cloned into a pUC19 vector by using electrocompetent Escherichia coli XL1-Blue MRF′ cells (Stratagene, Cedar Falls, Tex.). .. Transformants were checked for the presence of the bcr1 insert by restriction digests and DNA sequencing.

cDNA Library Assay:

Article Title: Cloning of a Phenol Oxidase Gene from Acremonium murorum and Its Expression in Aspergillus awamori
Article Snippet: Paragraph title: Construction of a cDNA library. ... Aliquots of 2.5 μl were used to transform 50 μl of electrocompetent E. coli XL1-Blue MRF′ cells (Stratagene) (conditions, 1,700 V, 200 ω, and 25 μF).

Transformation Assay:

Article Title: Identification of Mycobacterium avium Genes That Affect Invasion of the Intestinal Epithelium
Article Snippet: .. Cells were recovered with 1 ml 7H9 and incubated at 37°C with shaking for 2 h. Electrocompetent E. coli XL1-Blue MRF′ cells were either purchased from Stratagene or prepared and transformed as previously described ( ). ..

Plasmid Preparation:

Article Title: The bcr1 DNA Repeat Element Is Specific to the Bacillus cereus Group and Exhibits Mobile Element Characteristics
Article Snippet: .. Furthermore, a plot of the conservation of each site was generated by CLUSTALX ( bcr1 PCR products were purified with the QIAquick PCR purification kit (QIAGEN GmbH, Hilden, Germany) and cloned into a pUC19 vector by using electrocompetent Escherichia coli XL1-Blue MRF′ cells (Stratagene, Cedar Falls, Tex.). .. Transformants were checked for the presence of the bcr1 insert by restriction digests and DNA sequencing.

Article Title: Cloning of a Phenol Oxidase Gene from Acremonium murorum and Its Expression in Aspergillus awamori
Article Snippet: cDNA was cloned as Eco RI/ Xho I fragments into plasmid pYES2.0 (Invitrogen). .. Aliquots of 2.5 μl were used to transform 50 μl of electrocompetent E. coli XL1-Blue MRF′ cells (Stratagene) (conditions, 1,700 V, 200 ω, and 25 μF).

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    Stratagene electrocompetent e coli xl1 blue mrf cells
    Electrocompetent E Coli Xl1 Blue Mrf Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electrocompetent e coli xl1 blue mrf cells/product/Stratagene
    Average 86 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    electrocompetent e coli xl1 blue mrf cells - by Bioz Stars, 2020-04
    86/100 stars
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