electrocompetent e coli xl1 blue cells  (Thermo Fisher)


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    Structured Review

    Thermo Fisher electrocompetent e coli xl1 blue cells
    Electrocompetent E Coli Xl1 Blue Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electrocompetent e coli xl1 blue cells/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    electrocompetent e coli xl1 blue cells - by Bioz Stars, 2020-04
    86/100 stars

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    Clone Assay:

    Article Title: Human Monoclonal Fab Antibodies Against West Nile Virus and its Neutralizing Activity Analyzed in Vitro and in Vivo
    Article Snippet: Heavy chain Fd fragments were cut with excess of the restriction enzymes Xho I and Spe I and were cloned into Xho I / Spe I – linearized pComb3-H harboring light chains. .. The DNA pellet was transformed into electrocompetent E. coli XL1 -Blue cells (Invitrogen).

    Amplification:

    Article Title: Human Monoclonal Fab Antibodies Against West Nile Virus and its Neutralizing Activity Analyzed in Vitro and in Vivo
    Article Snippet: The amplified light chains were digested with XbaI and SacI , and purified by electrophoresis in 1.5% agarose gel. .. The DNA pellet was transformed into electrocompetent E. coli XL1 -Blue cells (Invitrogen).

    Agarose Gel Electrophoresis:

    Article Title: Human Monoclonal Fab Antibodies Against West Nile Virus and its Neutralizing Activity Analyzed in Vitro and in Vivo
    Article Snippet: The amplified light chains were digested with XbaI and SacI , and purified by electrophoresis in 1.5% agarose gel. .. The DNA pellet was transformed into electrocompetent E. coli XL1 -Blue cells (Invitrogen).

    Purification:

    Article Title: Human Monoclonal Fab Antibodies Against West Nile Virus and its Neutralizing Activity Analyzed in Vitro and in Vivo
    Article Snippet: Purified DNAs were ligated with XbaI/SacI - linearized pComb3-H vector (provided by the Scripps Research Institute). .. The DNA pellet was transformed into electrocompetent E. coli XL1 -Blue cells (Invitrogen).

    Electrophoresis:

    Article Title: Human Monoclonal Fab Antibodies Against West Nile Virus and its Neutralizing Activity Analyzed in Vitro and in Vivo
    Article Snippet: The amplified light chains were digested with XbaI and SacI , and purified by electrophoresis in 1.5% agarose gel. .. The DNA pellet was transformed into electrocompetent E. coli XL1 -Blue cells (Invitrogen).

    Expressing:

    Article Title: Human Monoclonal Fab Antibodies Against West Nile Virus and its Neutralizing Activity Analyzed in Vitro and in Vivo
    Article Snippet: Paragraph title: RNA Preparation and Construction of Expression Vector ... The DNA pellet was transformed into electrocompetent E. coli XL1 -Blue cells (Invitrogen).

    Gel Extraction:

    Article Title: Human Monoclonal Fab Antibodies Against West Nile Virus and its Neutralizing Activity Analyzed in Vitro and in Vivo
    Article Snippet: The relevant DNA bands were excised from the gels and extracted using a QIAquick gel extraction kit (Qiagen). .. The DNA pellet was transformed into electrocompetent E. coli XL1 -Blue cells (Invitrogen).

    Transformation Assay:

    Article Title: Human Monoclonal Fab Antibodies Against West Nile Virus and its Neutralizing Activity Analyzed in Vitro and in Vivo
    Article Snippet: .. The DNA pellet was transformed into electrocompetent E. coli XL1 -Blue cells (Invitrogen). .. After transformation, XL1 -blue -DNA mix (10, 1, 0.1 μl) was plated to determine the efficiency of transformation.

    Plasmid Preparation:

    Article Title: Human Monoclonal Fab Antibodies Against West Nile Virus and its Neutralizing Activity Analyzed in Vitro and in Vivo
    Article Snippet: Paragraph title: RNA Preparation and Construction of Expression Vector ... The DNA pellet was transformed into electrocompetent E. coli XL1 -Blue cells (Invitrogen).