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Stratagene electrocompetent e coli xl1 blue cells
Electrocompetent E Coli Xl1 Blue Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/electrocompetent e coli xl1 blue cells/product/Stratagene
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
electrocompetent e coli xl1 blue cells - by Bioz Stars, 2020-04
86/100 stars

Related Products / Commonly Used Together

dna pellet
pds596
pcr-purification kit

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Related Articles

Clone Assay:

Article Title: Alternative Conformations of the Tau Repeat Domain in Complex with an Engineered Binding Protein *
Article Snippet: Paragraph title: Cloning of the Library ... The library was transformed into electrocompetent E. coli XL1-Blue cells (Stratagene) resulting in 2.3 × 109 transformants.

Article Title: Identification of Bacteriocin-Like Inhibitors from Rumen Streptococcus spp. and Isolation and Characterization of Bovicin 255 †
Article Snippet: .. The 2.3-kbp region was purified from an agarose gel and cloned into pGEM7zf(−) (Promega Corp., Madison, Wis.) and transformed into electrocompetent Escherichia coli XL1-Blue cells (Stratagene) by electroporation according to the manufacturer's specifications.). .. The nucleotide sequence encoding bovicin 255 has been deposited in GenBank under accession number .

Sequencing:

Article Title: Alternative Conformations of the Tau Repeat Domain in Complex with an Engineered Binding Protein *
Article Snippet: In addition to the library insert, the pComb3HSS vector contained the coding sequence for c-Myc tag and albumin-binding domain from streptococcal protein G directly cloned between XhoI and SpeI. .. The library was transformed into electrocompetent E. coli XL1-Blue cells (Stratagene) resulting in 2.3 × 109 transformants.

Article Title: Identification of Bacteriocin-Like Inhibitors from Rumen Streptococcus spp. and Isolation and Characterization of Bovicin 255 †
Article Snippet: Southern blots containing restriction-digested genomic or plasmid DNA ( ) from LRC0255 were probed with an oligonucleotide based on the N-terminal sequence of the antibacterial peptide purified from LRC0255 (5′-AAA CCI GTI TAY GCI GCI AAY GGI TA-3′, where I represents inosine). .. The 2.3-kbp region was purified from an agarose gel and cloned into pGEM7zf(−) (Promega Corp., Madison, Wis.) and transformed into electrocompetent Escherichia coli XL1-Blue cells (Stratagene) by electroporation according to the manufacturer's specifications.).

Agarose Gel Electrophoresis:

Article Title: Identification of Bacteriocin-Like Inhibitors from Rumen Streptococcus spp. and Isolation and Characterization of Bovicin 255 †
Article Snippet: .. The 2.3-kbp region was purified from an agarose gel and cloned into pGEM7zf(−) (Promega Corp., Madison, Wis.) and transformed into electrocompetent Escherichia coli XL1-Blue cells (Stratagene) by electroporation according to the manufacturer's specifications.). .. The nucleotide sequence encoding bovicin 255 has been deposited in GenBank under accession number .

E. coli Genomic Assay:

Article Title: DnaAcos Hyperinitiates by Circumventing Regulatory Pathways that Control the Frequency of Initiation in Escherichia coli
Article Snippet: Paragraph title: Construction of an E. coli genomic library ... The ligation mixture was purified using a PCR-purification kit (Qiagen), concentrated by lyophilization, and electroporated with 20 ng of pDS596 into highly electrocompetent E. coli XL1-Blue cells (60 μl, Stratagene).

Ligation:

Article Title: DnaAcos Hyperinitiates by Circumventing Regulatory Pathways that Control the Frequency of Initiation in Escherichia coli
Article Snippet: .. The ligation mixture was purified using a PCR-purification kit (Qiagen), concentrated by lyophilization, and electroporated with 20 ng of pDS596 into highly electrocompetent E. coli XL1-Blue cells (60 μl, Stratagene). .. After incubation for 1 hr in SOC media at 37°C with aeration, most of the transformation mixture was plated on antibiotic-supplemented media containing L-arabinose to select for pACYC184 derivatives that suppressed the lethal effect caused by elevated dnaA expression.

Construct:

Article Title: Alternative Conformations of the Tau Repeat Domain in Complex with an Engineered Binding Protein *
Article Snippet: A new β-wrapin library was constructed by overlapping PCR using primers encoding AQHDEA peptide derived from the region E of protein A followed by the randomized ZAβ3 gene. .. The library was transformed into electrocompetent E. coli XL1-Blue cells (Stratagene) resulting in 2.3 × 109 transformants.

Purification:

Article Title: DnaAcos Hyperinitiates by Circumventing Regulatory Pathways that Control the Frequency of Initiation in Escherichia coli
Article Snippet: .. The ligation mixture was purified using a PCR-purification kit (Qiagen), concentrated by lyophilization, and electroporated with 20 ng of pDS596 into highly electrocompetent E. coli XL1-Blue cells (60 μl, Stratagene). .. After incubation for 1 hr in SOC media at 37°C with aeration, most of the transformation mixture was plated on antibiotic-supplemented media containing L-arabinose to select for pACYC184 derivatives that suppressed the lethal effect caused by elevated dnaA expression.

Article Title: Identification of Bacteriocin-Like Inhibitors from Rumen Streptococcus spp. and Isolation and Characterization of Bovicin 255 †
Article Snippet: .. The 2.3-kbp region was purified from an agarose gel and cloned into pGEM7zf(−) (Promega Corp., Madison, Wis.) and transformed into electrocompetent Escherichia coli XL1-Blue cells (Stratagene) by electroporation according to the manufacturer's specifications.). .. The nucleotide sequence encoding bovicin 255 has been deposited in GenBank under accession number .

Concentration Assay:

Article Title: DnaAcos Hyperinitiates by Circumventing Regulatory Pathways that Control the Frequency of Initiation in Escherichia coli
Article Snippet: From 3–8 min, portions were removed at one min time intervals, Na2 -EDTA was added to a final concentration of 20 mM, followed by incubation for 20 min at 65°C. .. The ligation mixture was purified using a PCR-purification kit (Qiagen), concentrated by lyophilization, and electroporated with 20 ng of pDS596 into highly electrocompetent E. coli XL1-Blue cells (60 μl, Stratagene).

Incubation:

Article Title: DnaAcos Hyperinitiates by Circumventing Regulatory Pathways that Control the Frequency of Initiation in Escherichia coli
Article Snippet: From 3–8 min, portions were removed at one min time intervals, Na2 -EDTA was added to a final concentration of 20 mM, followed by incubation for 20 min at 65°C. .. The ligation mixture was purified using a PCR-purification kit (Qiagen), concentrated by lyophilization, and electroporated with 20 ng of pDS596 into highly electrocompetent E. coli XL1-Blue cells (60 μl, Stratagene).

Plasmid Preparation:

Article Title: Alternative Conformations of the Tau Repeat Domain in Complex with an Engineered Binding Protein *
Article Snippet: In addition to the library insert, the pComb3HSS vector contained the coding sequence for c-Myc tag and albumin-binding domain from streptococcal protein G directly cloned between XhoI and SpeI. .. The library was transformed into electrocompetent E. coli XL1-Blue cells (Stratagene) resulting in 2.3 × 109 transformants.

Article Title: Identification of Bacteriocin-Like Inhibitors from Rumen Streptococcus spp. and Isolation and Characterization of Bovicin 255 †
Article Snippet: Plasmid DNA or restriction enzyme-digested genomic DNA was separated on an agarose gel and transferred to a Hybond N (Amersham Pharmacia) membrane by capillary transfer in 20× SSC (3 M NaCl, 0.3 M trisodium citrate; pH 7.0). .. The 2.3-kbp region was purified from an agarose gel and cloned into pGEM7zf(−) (Promega Corp., Madison, Wis.) and transformed into electrocompetent Escherichia coli XL1-Blue cells (Stratagene) by electroporation according to the manufacturer's specifications.).

Expressing:

Article Title: DnaAcos Hyperinitiates by Circumventing Regulatory Pathways that Control the Frequency of Initiation in Escherichia coli
Article Snippet: The ligation mixture was purified using a PCR-purification kit (Qiagen), concentrated by lyophilization, and electroporated with 20 ng of pDS596 into highly electrocompetent E. coli XL1-Blue cells (60 μl, Stratagene). .. After incubation for 1 hr in SOC media at 37°C with aeration, most of the transformation mixture was plated on antibiotic-supplemented media containing L-arabinose to select for pACYC184 derivatives that suppressed the lethal effect caused by elevated dnaA expression.

Electroporation:

Article Title: Identification of Bacteriocin-Like Inhibitors from Rumen Streptococcus spp. and Isolation and Characterization of Bovicin 255 †
Article Snippet: .. The 2.3-kbp region was purified from an agarose gel and cloned into pGEM7zf(−) (Promega Corp., Madison, Wis.) and transformed into electrocompetent Escherichia coli XL1-Blue cells (Stratagene) by electroporation according to the manufacturer's specifications.). .. The nucleotide sequence encoding bovicin 255 has been deposited in GenBank under accession number .

Polymerase Chain Reaction:

Article Title: Alternative Conformations of the Tau Repeat Domain in Complex with an Engineered Binding Protein *
Article Snippet: A new β-wrapin library was constructed by overlapping PCR using primers encoding AQHDEA peptide derived from the region E of protein A followed by the randomized ZAβ3 gene. .. The library was transformed into electrocompetent E. coli XL1-Blue cells (Stratagene) resulting in 2.3 × 109 transformants.

Article Title: DnaAcos Hyperinitiates by Circumventing Regulatory Pathways that Control the Frequency of Initiation in Escherichia coli
Article Snippet: .. The ligation mixture was purified using a PCR-purification kit (Qiagen), concentrated by lyophilization, and electroporated with 20 ng of pDS596 into highly electrocompetent E. coli XL1-Blue cells (60 μl, Stratagene). .. After incubation for 1 hr in SOC media at 37°C with aeration, most of the transformation mixture was plated on antibiotic-supplemented media containing L-arabinose to select for pACYC184 derivatives that suppressed the lethal effect caused by elevated dnaA expression.

Sonication:

Article Title: Identification of Bacteriocin-Like Inhibitors from Rumen Streptococcus spp. and Isolation and Characterization of Bovicin 255 †
Article Snippet: Blots were hybridized overnight at 40°C in hybridization solution (5× SSC, 0.1% SDS, 10 mg of sonicated denatured fish sperm DNA per ml) containing 5 pmol of a 32 P-end-labeled oligonucleotide probe. .. The 2.3-kbp region was purified from an agarose gel and cloned into pGEM7zf(−) (Promega Corp., Madison, Wis.) and transformed into electrocompetent Escherichia coli XL1-Blue cells (Stratagene) by electroporation according to the manufacturer's specifications.).

Transformation Assay:

Article Title: Alternative Conformations of the Tau Repeat Domain in Complex with an Engineered Binding Protein *
Article Snippet: .. The library was transformed into electrocompetent E. coli XL1-Blue cells (Stratagene) resulting in 2.3 × 109 transformants. .. The phage library was produced by superinfection of bacteria harboring the library with M13KO7 phage (New England Biolabs) and precipitation by PEG/NaCl.

Article Title: DnaAcos Hyperinitiates by Circumventing Regulatory Pathways that Control the Frequency of Initiation in Escherichia coli
Article Snippet: The ligation mixture was purified using a PCR-purification kit (Qiagen), concentrated by lyophilization, and electroporated with 20 ng of pDS596 into highly electrocompetent E. coli XL1-Blue cells (60 μl, Stratagene). .. After incubation for 1 hr in SOC media at 37°C with aeration, most of the transformation mixture was plated on antibiotic-supplemented media containing L-arabinose to select for pACYC184 derivatives that suppressed the lethal effect caused by elevated dnaA expression.

Article Title: Identification of Bacteriocin-Like Inhibitors from Rumen Streptococcus spp. and Isolation and Characterization of Bovicin 255 †
Article Snippet: .. The 2.3-kbp region was purified from an agarose gel and cloned into pGEM7zf(−) (Promega Corp., Madison, Wis.) and transformed into electrocompetent Escherichia coli XL1-Blue cells (Stratagene) by electroporation according to the manufacturer's specifications.). .. The nucleotide sequence encoding bovicin 255 has been deposited in GenBank under accession number .

Fluorescence In Situ Hybridization:

Article Title: Identification of Bacteriocin-Like Inhibitors from Rumen Streptococcus spp. and Isolation and Characterization of Bovicin 255 †
Article Snippet: Blots were hybridized overnight at 40°C in hybridization solution (5× SSC, 0.1% SDS, 10 mg of sonicated denatured fish sperm DNA per ml) containing 5 pmol of a 32 P-end-labeled oligonucleotide probe. .. The 2.3-kbp region was purified from an agarose gel and cloned into pGEM7zf(−) (Promega Corp., Madison, Wis.) and transformed into electrocompetent Escherichia coli XL1-Blue cells (Stratagene) by electroporation according to the manufacturer's specifications.).

Derivative Assay:

Article Title: Alternative Conformations of the Tau Repeat Domain in Complex with an Engineered Binding Protein *
Article Snippet: A new β-wrapin library was constructed by overlapping PCR using primers encoding AQHDEA peptide derived from the region E of protein A followed by the randomized ZAβ3 gene. .. The library was transformed into electrocompetent E. coli XL1-Blue cells (Stratagene) resulting in 2.3 × 109 transformants.

Hybridization:

Article Title: Identification of Bacteriocin-Like Inhibitors from Rumen Streptococcus spp. and Isolation and Characterization of Bovicin 255 †
Article Snippet: Blots were hybridized overnight at 40°C in hybridization solution (5× SSC, 0.1% SDS, 10 mg of sonicated denatured fish sperm DNA per ml) containing 5 pmol of a 32 P-end-labeled oligonucleotide probe. .. The 2.3-kbp region was purified from an agarose gel and cloned into pGEM7zf(−) (Promega Corp., Madison, Wis.) and transformed into electrocompetent Escherichia coli XL1-Blue cells (Stratagene) by electroporation according to the manufacturer's specifications.).

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    Stratagene electrocompetent e coli xl1 blue cells
    Electrocompetent E Coli Xl1 Blue Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electrocompetent e coli xl1 blue cells/product/Stratagene
    Average 86 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    electrocompetent e coli xl1 blue cells - by Bioz Stars, 2020-04
    86/100 stars
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