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Setup of non-invasive Doppler OphA blood flow velocity measurements at mouse orbital opening An anesthetized mouse is placed in a prone position on the ECG board. The Doppler probe sensor tip is at the temporal canthus (left and center). Doppler in-phase (I), quadrature (Q), and ECG signals are acquired, processed, displayed, and recorded in real-time (middle). Doppler velocity signal waveforms with major parameters are acquired for analysis (right). DVFS, Doppler flow velocity system; US, ultrasound; ECG, electrocardiogram; FV, flow velocity.

Journal: Cell Reports Methods

Article Title: Non-invasive real-time pulsed Doppler assessment of blood flow in mouse ophthalmic artery

doi: 10.1016/j.crmeth.2025.100983

Figure Lengend Snippet: Setup of non-invasive Doppler OphA blood flow velocity measurements at mouse orbital opening An anesthetized mouse is placed in a prone position on the ECG board. The Doppler probe sensor tip is at the temporal canthus (left and center). Doppler in-phase (I), quadrature (Q), and ECG signals are acquired, processed, displayed, and recorded in real-time (middle). Doppler velocity signal waveforms with major parameters are acquired for analysis (right). DVFS, Doppler flow velocity system; US, ultrasound; ECG, electrocardiogram; FV, flow velocity.

Article Snippet: The principle of operation of the DFVS is described in great detail elsewhere., , Mice were anesthetized with 2.0% isoflurane in the induction chamber and then transferred to a heated electrocardiogram (ECG) board (Mouse Monitor S, Indus Instruments, Webster, TX).

Techniques:

Targeted artery (OphA) originates as a branch off the ICA (A) Experimental setup for sequential ECA and ICA branch occlusion. A surgically prepared mouse on the heated board with continuous ECG monitoring, the Doppler probe, and the dissected area are shown. (B) Magnified view of the neck region prepared for occlusion. CCA, common carotid artery; ICA, internal carotid artery; ECA, external carotid artery. (C) Schematic representation of anatomical features in close proximity to CCA and ICA/ECA branching site (adapted from Liu et al. ). (D) Changes of the Doppler signal during sequential occlusion of the blood flow in left ECA and ICA. A representative of 6 independent sequential occlusions from 3 individual mice is shown.

Journal: Cell Reports Methods

Article Title: Non-invasive real-time pulsed Doppler assessment of blood flow in mouse ophthalmic artery

doi: 10.1016/j.crmeth.2025.100983

Figure Lengend Snippet: Targeted artery (OphA) originates as a branch off the ICA (A) Experimental setup for sequential ECA and ICA branch occlusion. A surgically prepared mouse on the heated board with continuous ECG monitoring, the Doppler probe, and the dissected area are shown. (B) Magnified view of the neck region prepared for occlusion. CCA, common carotid artery; ICA, internal carotid artery; ECA, external carotid artery. (C) Schematic representation of anatomical features in close proximity to CCA and ICA/ECA branching site (adapted from Liu et al. ). (D) Changes of the Doppler signal during sequential occlusion of the blood flow in left ECA and ICA. A representative of 6 independent sequential occlusions from 3 individual mice is shown.

Article Snippet: The principle of operation of the DFVS is described in great detail elsewhere., , Mice were anesthetized with 2.0% isoflurane in the induction chamber and then transferred to a heated electrocardiogram (ECG) board (Mouse Monitor S, Indus Instruments, Webster, TX).

Techniques: