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Merck & Co egta
Egta, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/egta/product/Merck & Co
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
egta - by Bioz Stars, 2021-03
86/100 stars

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Related Articles

Transferring:

Article Title: Sphingosine-1-Phosphate-Induced Nociceptor Excitation and Ongoing Pain Behavior in Mice and Humans Is Largely Mediated by S1P3 Receptor
Article Snippet: The membrane potential was recorded using current-clamp configuration (Ihold = 0 pA) using an ECS containing the following (in m m ): 145 NaCl, 5 KCl, 2 CaCl2 , 1 MgCl2 (all Sigma), 10 d -glucose and 10 HEPES (Merck), at pH 7.3 adjusted with NaOH (Merck). .. The pipette solution was composed (in m m ) of 45 KCl, 98 K-gluconate, 0.5 CaCl2 , 5 EGTA, 10 HEPES, 2 MgATP, 0.2 NaGTP, pH 7.3 adjusted with KOH (Merck). ..

Incubation:

Article Title: The Potential Impact of Connexin 43 Expression on Bcl-2 Protein Level and Taxane Sensitivity in Head and Neck Cancers–In Vitro Studies
Article Snippet: Western Blot Analysis Cells were grown until 90% confluence in 6 well plates and incubated for 48 h in medium. .. After incubation, cells were washed with ice-cold PBS and lysed in lysis buffer (50 mM Tris (pH 7.4), 150 mM NaCl, 1% (V/V) NP-40, 2 mM EDTA, 2 mM EGTA, 1 mM dithiothreitol, phosphatase inhibitor cocktail (Merck, Kenilworth, NJ, USA) and protease inhibitor cocktail (Calbiochem)) for 30 min on ice. .. Lysates were centrifuged with 13,000× g at 4 °C for 15 min. Then, 10 μg protein samples were subjected to SDS-PAGE and electrotransferred to polyvinylidene-difluoride (PVDF) membranes.

Lysis:

Article Title: The Potential Impact of Connexin 43 Expression on Bcl-2 Protein Level and Taxane Sensitivity in Head and Neck Cancers–In Vitro Studies
Article Snippet: Western Blot Analysis Cells were grown until 90% confluence in 6 well plates and incubated for 48 h in medium. .. After incubation, cells were washed with ice-cold PBS and lysed in lysis buffer (50 mM Tris (pH 7.4), 150 mM NaCl, 1% (V/V) NP-40, 2 mM EDTA, 2 mM EGTA, 1 mM dithiothreitol, phosphatase inhibitor cocktail (Merck, Kenilworth, NJ, USA) and protease inhibitor cocktail (Calbiochem)) for 30 min on ice. .. Lysates were centrifuged with 13,000× g at 4 °C for 15 min. Then, 10 μg protein samples were subjected to SDS-PAGE and electrotransferred to polyvinylidene-difluoride (PVDF) membranes.

Protease Inhibitor:

Article Title: The Potential Impact of Connexin 43 Expression on Bcl-2 Protein Level and Taxane Sensitivity in Head and Neck Cancers–In Vitro Studies
Article Snippet: Western Blot Analysis Cells were grown until 90% confluence in 6 well plates and incubated for 48 h in medium. .. After incubation, cells were washed with ice-cold PBS and lysed in lysis buffer (50 mM Tris (pH 7.4), 150 mM NaCl, 1% (V/V) NP-40, 2 mM EDTA, 2 mM EGTA, 1 mM dithiothreitol, phosphatase inhibitor cocktail (Merck, Kenilworth, NJ, USA) and protease inhibitor cocktail (Calbiochem)) for 30 min on ice. .. Lysates were centrifuged with 13,000× g at 4 °C for 15 min. Then, 10 μg protein samples were subjected to SDS-PAGE and electrotransferred to polyvinylidene-difluoride (PVDF) membranes.

Injection:

Article Title: Xenotransplanted Human Cortical Neurons Reveal Species-Specific Development and Functional Integration into Mouse Visual Circuits
Article Snippet: The following day, cells were treated with 5 μM Cytarabine (Merck, Cat#C3350000) for 24 hours. .. At day 17-19 days after thawing, cells were dissociated using Accutase and suspended in the injection solution containing 20 mM EGTA (Merck, Cat03777) and 0.1% Fast Green (Merck, Cat#210-M) in PBS at 40,000–200,000 cells/μl. .. Approximately 1-2 μl of cell suspension was injected into the lateral ventricles of each hemisphere of neonatal (postnatal day 0 or 1) immunodeficient mice (NOD/SCID or Rag2−/− ) using glass capillaries pulled on a horizontal puller (Sutter P-97).

Chloramphenicol Acetyltransferase Assay:

Article Title: Xenotransplanted Human Cortical Neurons Reveal Species-Specific Development and Functional Integration into Mouse Visual Circuits
Article Snippet: The following day, cells were treated with 5 μM Cytarabine (Merck, Cat#C3350000) for 24 hours. .. At day 17-19 days after thawing, cells were dissociated using Accutase and suspended in the injection solution containing 20 mM EGTA (Merck, Cat03777) and 0.1% Fast Green (Merck, Cat#210-M) in PBS at 40,000–200,000 cells/μl. .. Approximately 1-2 μl of cell suspension was injected into the lateral ventricles of each hemisphere of neonatal (postnatal day 0 or 1) immunodeficient mice (NOD/SCID or Rag2−/− ) using glass capillaries pulled on a horizontal puller (Sutter P-97).

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    Merck & Co egta
    Effect of Ca 2+ chelation and Ca 2+ channel inhibitors on HMR 3647 uptake. (A) PMNs were incubated for 5 to 60 min in the presence of 1 mM <t>EGTA,</t> 5 mM Ni 2+ , 125 μM verapamil (VPL), or control <t>HBSS.</t> Results are means ± SEM for four to six experiments. ∗, P
    Egta, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta/product/Merck & Co
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    egta - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Merck & Co ethylene glycol tetraacetic acid egta
    Effect of Ca 2+ chelation and Ca 2+ channel inhibitors on HMR 3647 uptake. (A) PMNs were incubated for 5 to 60 min in the presence of 1 mM <t>EGTA,</t> 5 mM Ni 2+ , 125 μM verapamil (VPL), or control <t>HBSS.</t> Results are means ± SEM for four to six experiments. ∗, P
    Ethylene Glycol Tetraacetic Acid Egta, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethylene glycol tetraacetic acid egta/product/Merck & Co
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ethylene glycol tetraacetic acid egta - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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    Effect of Ca 2+ chelation and Ca 2+ channel inhibitors on HMR 3647 uptake. (A) PMNs were incubated for 5 to 60 min in the presence of 1 mM EGTA, 5 mM Ni 2+ , 125 μM verapamil (VPL), or control HBSS. Results are means ± SEM for four to six experiments. ∗, P

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Interactions between HMR 3647, a New Ketolide, and Human Polymorphonuclear Neutrophils

    doi:

    Figure Lengend Snippet: Effect of Ca 2+ chelation and Ca 2+ channel inhibitors on HMR 3647 uptake. (A) PMNs were incubated for 5 to 60 min in the presence of 1 mM EGTA, 5 mM Ni 2+ , 125 μM verapamil (VPL), or control HBSS. Results are means ± SEM for four to six experiments. ∗, P

    Article Snippet: The uptake kinetics of macrolides was assessed first in Ca2+ -depleted HBSS (Gibco), supplemented with 1 mM EGTA (Merck), 1 mM magnesium chloride (Merck), and 4.2 mM sodium bicarbonate (NaHCO3 ) (Diagnostic Pasteur).

    Techniques: Incubation

    Protoplast shrinkage at 55°C HS is an ATP- and Ca 2+ -independent process. A Mitochondria in BY-2 cells stained with MitoTracker Red and imaged 10 min after 55°C or 85°C HS, or after treatment with 48 µM CCCP. Severely damaged mitochondria were observed upon all three treatments. B Loss of intracellular ATP content upon HS. Snap freeze-thaw treatment in liquid nitrogen (N 2 ) and CCCP treatment were used as positive controls for completely disrupted and uncoupled mitochondria, respectively. The experiment was repeated twice, each time using four biological replicates per treatment. C MitoTracker Red staining of BY-2 cells exposed to 55°C in the presence or absence of 15 µM Cyclosporin A (CsA) reveals that inhibition of MPTP opening does not rescue mitochondria from severe damage and loss of membrane potential caused by HS. D MitoTracker Red localization in the cells pre-treated with 10 mM EGTA prior to the HS reveals that chelation of extracellular Ca 2+ does not rescue mitochondrial phenotype. E, F Dynamicsof cell death (% SO-positive cells; E ) and protoplast shrinkage (F) in cells with normal and uncoupled (48 µM CCCP treatment) mitochondria. G, H Pre-treatment with 10 mM EGTA before HS does not affect dynamics of cell death (% SO-positive cells; G ) and protoplast shrinkage (H) . Experiments shown in E-H were repeated three times, with ≥ 184 cells per treatment and time point. Each microscopy experiment was performed at least twice. Scale bars, 20 µm ( A ) or 50 µm ( C, D ). IQR, interquartile range. B, E - H , one-way ANOVA with Dunnet’s test; *, p

    Journal: bioRxiv

    Article Title: Apoptosis in plants: from semantic appeal to empirical rejection

    doi: 10.1101/2020.09.26.314583

    Figure Lengend Snippet: Protoplast shrinkage at 55°C HS is an ATP- and Ca 2+ -independent process. A Mitochondria in BY-2 cells stained with MitoTracker Red and imaged 10 min after 55°C or 85°C HS, or after treatment with 48 µM CCCP. Severely damaged mitochondria were observed upon all three treatments. B Loss of intracellular ATP content upon HS. Snap freeze-thaw treatment in liquid nitrogen (N 2 ) and CCCP treatment were used as positive controls for completely disrupted and uncoupled mitochondria, respectively. The experiment was repeated twice, each time using four biological replicates per treatment. C MitoTracker Red staining of BY-2 cells exposed to 55°C in the presence or absence of 15 µM Cyclosporin A (CsA) reveals that inhibition of MPTP opening does not rescue mitochondria from severe damage and loss of membrane potential caused by HS. D MitoTracker Red localization in the cells pre-treated with 10 mM EGTA prior to the HS reveals that chelation of extracellular Ca 2+ does not rescue mitochondrial phenotype. E, F Dynamicsof cell death (% SO-positive cells; E ) and protoplast shrinkage (F) in cells with normal and uncoupled (48 µM CCCP treatment) mitochondria. G, H Pre-treatment with 10 mM EGTA before HS does not affect dynamics of cell death (% SO-positive cells; G ) and protoplast shrinkage (H) . Experiments shown in E-H were repeated three times, with ≥ 184 cells per treatment and time point. Each microscopy experiment was performed at least twice. Scale bars, 20 µm ( A ) or 50 µm ( C, D ). IQR, interquartile range. B, E - H , one-way ANOVA with Dunnet’s test; *, p

    Article Snippet: Four different treatments (all at room temperature) were applied prior to 10-min HS at 55°C: (i) 10 mM EGTA (Merck, E3889), pH 8.0 for 10 min, (ii) 0.1% DMSO for 2 h, (iii) 15 µM cyclosporin A (CsA) for 2 h, and (iv) 10 mM EGTA (applied 10 min prior to HS) and 15 µM CsA (applied 2 h prior to HS).

    Techniques: Staining, Inhibition, Microscopy

    HS-induced cell death is ATP- and Ca 2+ -independent process. A-D Morphology of FDA-stained cells under normal conditions (no HS) and after a 55°C HS. Protoplast shrinkage is denoted by arrows. Pre-treatment with 48 µM CCCP for 10 min (B) , 15 µM CsA for 2 h (C ), or 10 mM EGTA for 10 min ( D ) did not alleviate protoplast shrinkage upon HS. Each treatment was repeated at least twice. E, G Sytox Orange (SO) staining of BY-2 cells heat-shocked for 10 min at 40, 45, or 50°C and imaged after 6 h ( E ) and 24 h ( G ). Pre-treatment with CCCP provided no protection against cell death and protoplast shrinkage at any of the tested HS temperatures. F, H Quantification of cell death (% SO-positive cells) in the samples shown in E and G , respectively. DIC, differential interference contrast microscopy. IQR, interquartile range. Experiments shown in F and H were repeated three times, with ≥ 170 cells per treatment and time point. The data was subjected to one-way ANOVA with Bonferroni correction. Scale bars, 20 µm (A-D) or 100 µm (E, G) .

    Journal: bioRxiv

    Article Title: Apoptosis in plants: from semantic appeal to empirical rejection

    doi: 10.1101/2020.09.26.314583

    Figure Lengend Snippet: HS-induced cell death is ATP- and Ca 2+ -independent process. A-D Morphology of FDA-stained cells under normal conditions (no HS) and after a 55°C HS. Protoplast shrinkage is denoted by arrows. Pre-treatment with 48 µM CCCP for 10 min (B) , 15 µM CsA for 2 h (C ), or 10 mM EGTA for 10 min ( D ) did not alleviate protoplast shrinkage upon HS. Each treatment was repeated at least twice. E, G Sytox Orange (SO) staining of BY-2 cells heat-shocked for 10 min at 40, 45, or 50°C and imaged after 6 h ( E ) and 24 h ( G ). Pre-treatment with CCCP provided no protection against cell death and protoplast shrinkage at any of the tested HS temperatures. F, H Quantification of cell death (% SO-positive cells) in the samples shown in E and G , respectively. DIC, differential interference contrast microscopy. IQR, interquartile range. Experiments shown in F and H were repeated three times, with ≥ 170 cells per treatment and time point. The data was subjected to one-way ANOVA with Bonferroni correction. Scale bars, 20 µm (A-D) or 100 µm (E, G) .

    Article Snippet: Four different treatments (all at room temperature) were applied prior to 10-min HS at 55°C: (i) 10 mM EGTA (Merck, E3889), pH 8.0 for 10 min, (ii) 0.1% DMSO for 2 h, (iii) 15 µM cyclosporin A (CsA) for 2 h, and (iv) 10 mM EGTA (applied 10 min prior to HS) and 15 µM CsA (applied 2 h prior to HS).

    Techniques: Staining, Microscopy