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Boston BioProducts egta
Egta, supplied by Boston BioProducts, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/egta/product/Boston BioProducts
Average 92 stars, based on 9 article reviews
Price from $9.99 to $1999.99
egta - by Bioz Stars, 2020-07
92/100 stars

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Radio Immunoprecipitation:

Article Title: Respiration: Role of miR-29 in mediating offspring lung phenotype in a rodent model of intrauterine growth restriction
Article Snippet: .. Liquid nitrogen flash-frozen lung tissue samples and fetal lung fibroblasts following treatment conditions were suspended in radioimmunoprecipitation assay buffer containing 1 mM EDTA and EGTA (Boston BioProducts, Ashland, MA) supplemented with 1 mM PMSF and a complete protease inhibitor mixture (Roche Diagnostics, Indianapolis, IN), sonicated, and centrifuged at 4°C for 10 min at 14,000 rpm. .. The concentration of protein was determined using the BCA Protein Assay Kit (Thermo Scientific Pierce, Rockford, IL).

Protease Inhibitor:

Article Title: Discovery of piperlongumine as a potential novel lead for the development of senolytic agents
Article Snippet: .. Western blot analysis Cells were lysed in RIPA buffer with EDTA and EGTA (catalog no. BP-115DG, Boston BioProducts, Ashland, MA), supplemented with 1% Phosphatase Inhibitor Cocktail 3 (catalog no. P0044, Sigma-Aldrich) and 1% Protease Inhibitor Cocktail (catalog no. P8340, Sigma-Aldrich). ..

Article Title: Identification of genes regulating migration and invasion using a new model of metastatic prostate cancer
Article Snippet: .. Western blot analysis Whole cell lysates were collected in modified RIPA buffer with EGTA and EDTA (Boston Bioproducts, Ashland, MA) with protease inhibitor cocktail (P8340, Sigma-Aldrich). .. Conditioned media was collected from serum-free cell cultures, cells removed by centrifugation at 200 × g and protein concentrated using Amicon Ultra-15 3 kDa Centrifugal Filter Units (Millipore) at 3000 × g. Protein concentration was measured using a BCA (bicinchoninic acid) assay kit (Pierce/Thermo Scientific).

Article Title: Respiration: Role of miR-29 in mediating offspring lung phenotype in a rodent model of intrauterine growth restriction
Article Snippet: .. Liquid nitrogen flash-frozen lung tissue samples and fetal lung fibroblasts following treatment conditions were suspended in radioimmunoprecipitation assay buffer containing 1 mM EDTA and EGTA (Boston BioProducts, Ashland, MA) supplemented with 1 mM PMSF and a complete protease inhibitor mixture (Roche Diagnostics, Indianapolis, IN), sonicated, and centrifuged at 4°C for 10 min at 14,000 rpm. .. The concentration of protein was determined using the BCA Protein Assay Kit (Thermo Scientific Pierce, Rockford, IL).

Immunoprecipitation:

Article Title: Resveratrol improves adipose insulin signaling and reduces the inflammatory response in adipose tissue of rhesus monkeys on a high-fat, high-sugar diet
Article Snippet: .. For immunoprecipitation and determination of GLUT4 and TUG protein content, visceral adipose tissue was mechanically lysed and briefly sonicated in RIPA buffer supplemented with EDTA and EGTA (Boston BioProducts, Ashland, MA), protease and phosphatase inhibitors (Sigma-Aldrich) and 0.1 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich). ..

Western Blot:

Article Title: Discovery of piperlongumine as a potential novel lead for the development of senolytic agents
Article Snippet: .. Western blot analysis Cells were lysed in RIPA buffer with EDTA and EGTA (catalog no. BP-115DG, Boston BioProducts, Ashland, MA), supplemented with 1% Phosphatase Inhibitor Cocktail 3 (catalog no. P0044, Sigma-Aldrich) and 1% Protease Inhibitor Cocktail (catalog no. P8340, Sigma-Aldrich). ..

Article Title: Identification of genes regulating migration and invasion using a new model of metastatic prostate cancer
Article Snippet: .. Western blot analysis Whole cell lysates were collected in modified RIPA buffer with EGTA and EDTA (Boston Bioproducts, Ashland, MA) with protease inhibitor cocktail (P8340, Sigma-Aldrich). .. Conditioned media was collected from serum-free cell cultures, cells removed by centrifugation at 200 × g and protein concentrated using Amicon Ultra-15 3 kDa Centrifugal Filter Units (Millipore) at 3000 × g. Protein concentration was measured using a BCA (bicinchoninic acid) assay kit (Pierce/Thermo Scientific).

Modification:

Article Title: Identification of genes regulating migration and invasion using a new model of metastatic prostate cancer
Article Snippet: .. Western blot analysis Whole cell lysates were collected in modified RIPA buffer with EGTA and EDTA (Boston Bioproducts, Ashland, MA) with protease inhibitor cocktail (P8340, Sigma-Aldrich). .. Conditioned media was collected from serum-free cell cultures, cells removed by centrifugation at 200 × g and protein concentrated using Amicon Ultra-15 3 kDa Centrifugal Filter Units (Millipore) at 3000 × g. Protein concentration was measured using a BCA (bicinchoninic acid) assay kit (Pierce/Thermo Scientific).

Sonication:

Article Title: Respiration: Role of miR-29 in mediating offspring lung phenotype in a rodent model of intrauterine growth restriction
Article Snippet: .. Liquid nitrogen flash-frozen lung tissue samples and fetal lung fibroblasts following treatment conditions were suspended in radioimmunoprecipitation assay buffer containing 1 mM EDTA and EGTA (Boston BioProducts, Ashland, MA) supplemented with 1 mM PMSF and a complete protease inhibitor mixture (Roche Diagnostics, Indianapolis, IN), sonicated, and centrifuged at 4°C for 10 min at 14,000 rpm. .. The concentration of protein was determined using the BCA Protein Assay Kit (Thermo Scientific Pierce, Rockford, IL).

Article Title: Resveratrol improves adipose insulin signaling and reduces the inflammatory response in adipose tissue of rhesus monkeys on a high-fat, high-sugar diet
Article Snippet: .. For immunoprecipitation and determination of GLUT4 and TUG protein content, visceral adipose tissue was mechanically lysed and briefly sonicated in RIPA buffer supplemented with EDTA and EGTA (Boston BioProducts, Ashland, MA), protease and phosphatase inhibitors (Sigma-Aldrich) and 0.1 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich). ..

Lysis:

Article Title: Hyperoxia-induced neonatal rat lung injury involves activation of TGF-? and Wnt signaling and is protected by rosiglitazone
Article Snippet: .. Liquid nitrogen flash-frozen lung tissue was homogenized with a tissue grinder in lysis buffer (RIPA buffer) containing 1 mM EDTA and EGTA each (Boston Bioproducts), supplemented with 1 mM PMSF, phosphatase inhibitor (Sigma-Aldrich), and complete proteinase inhibitor cocktail (Roche Diagnostics). .. Fifty micrograms of total protein for each sample was denatured by SDS-PAGE sample buffer and electrophoresed in a 10% SDS polyacrylamide gel.

Article Title: Nicotinamide improves aspects of healthspan but not lifespan in mice
Article Snippet: .. Tissues were lysed either in radioimmunoprecipitation buffer (RIPA) or urea lysis buffer supplemented with ethylenediaminetetraacetic acid and ethylene glycol tetraacetic acid (Boston BioProducts, Ashland, MA) along with KDAC inhibitors [10 μM trichostatin A, 10 mM NAM, and 50 mM butyric acid, all from Sigma-Aldrich], and protease and phosphatase inhibitors (Roche, Indianapolis, IN). .. Following homogenization with a polytron homogenizer, samples were centrifuged (14,000 rpm, 30 min at 4°C) and protein concentration in the clarified lysates was then quantified using the Bradford assay method (Bio-Rad).

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    Boston BioProducts egta
    Egta, supplied by Boston BioProducts, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta/product/Boston BioProducts
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    egta - by Bioz Stars, 2020-07
    92/100 stars
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