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Amresco egta
The effects of different treatments on the biophotonic activities in mouse coronal brain slices. ( A–D ) Removing extracellular Ca 2+ ( A and B , 0.5 mM <t>EGTA,</t> n = 6) or removing intra- and extracellular Ca 2+ together ( C and D , 10 µM <t>BAPTA-AM+0.5</t> mM EGTA, n = 6) from the beginning of the application of 50 mM glutamate (pink line in A and C ). The changes were significant during the maintenance period ( B and D ). ( E, F ) 1 µM TTX had no influence on the initiation, but partly on the maintenance (pink line, n = 5). ( G, H ) Both initiation (green line, n = 5) and maintenance (pink line, n = 6) were significantly affected by 0.5% procaine. ( I, J ) Both initiation (green line, n = 6) and maintenance (pink line, n = 6) were significantly affected by 0.05% sodium azide (SA), but a recovery was found after long-lasting application. Arrows indicate the treatment at the beginning of the application of 50 mM glutamate in A , C , E , G and I and arrowheads mark the treatment that began after the achievement of the maximum effect in G and I . t: treated group; c: control group (blue line) is same in A–J (n = 6), i : the treatment together with application of glutamate (initiating period), m : the treatment during the maintenance period. Data show mean±s.e.m. n = the number of slices from the same number of mice. * treated group (corresponding color) versus control at the same time periods; + the maximum effect just before treatment versus the effects after (arrows in B , D , F , J ) in treated group. * or + P
Egta, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Spatiotemporal Imaging of Glutamate-Induced Biophotonic Activities and Transmission in Neural Circuits"

Article Title: Spatiotemporal Imaging of Glutamate-Induced Biophotonic Activities and Transmission in Neural Circuits

Journal: PLoS ONE

doi: 10.1371/journal.pone.0085643

The effects of different treatments on the biophotonic activities in mouse coronal brain slices. ( A–D ) Removing extracellular Ca 2+ ( A and B , 0.5 mM EGTA, n = 6) or removing intra- and extracellular Ca 2+ together ( C and D , 10 µM BAPTA-AM+0.5 mM EGTA, n = 6) from the beginning of the application of 50 mM glutamate (pink line in A and C ). The changes were significant during the maintenance period ( B and D ). ( E, F ) 1 µM TTX had no influence on the initiation, but partly on the maintenance (pink line, n = 5). ( G, H ) Both initiation (green line, n = 5) and maintenance (pink line, n = 6) were significantly affected by 0.5% procaine. ( I, J ) Both initiation (green line, n = 6) and maintenance (pink line, n = 6) were significantly affected by 0.05% sodium azide (SA), but a recovery was found after long-lasting application. Arrows indicate the treatment at the beginning of the application of 50 mM glutamate in A , C , E , G and I and arrowheads mark the treatment that began after the achievement of the maximum effect in G and I . t: treated group; c: control group (blue line) is same in A–J (n = 6), i : the treatment together with application of glutamate (initiating period), m : the treatment during the maintenance period. Data show mean±s.e.m. n = the number of slices from the same number of mice. * treated group (corresponding color) versus control at the same time periods; + the maximum effect just before treatment versus the effects after (arrows in B , D , F , J ) in treated group. * or + P
Figure Legend Snippet: The effects of different treatments on the biophotonic activities in mouse coronal brain slices. ( A–D ) Removing extracellular Ca 2+ ( A and B , 0.5 mM EGTA, n = 6) or removing intra- and extracellular Ca 2+ together ( C and D , 10 µM BAPTA-AM+0.5 mM EGTA, n = 6) from the beginning of the application of 50 mM glutamate (pink line in A and C ). The changes were significant during the maintenance period ( B and D ). ( E, F ) 1 µM TTX had no influence on the initiation, but partly on the maintenance (pink line, n = 5). ( G, H ) Both initiation (green line, n = 5) and maintenance (pink line, n = 6) were significantly affected by 0.5% procaine. ( I, J ) Both initiation (green line, n = 6) and maintenance (pink line, n = 6) were significantly affected by 0.05% sodium azide (SA), but a recovery was found after long-lasting application. Arrows indicate the treatment at the beginning of the application of 50 mM glutamate in A , C , E , G and I and arrowheads mark the treatment that began after the achievement of the maximum effect in G and I . t: treated group; c: control group (blue line) is same in A–J (n = 6), i : the treatment together with application of glutamate (initiating period), m : the treatment during the maintenance period. Data show mean±s.e.m. n = the number of slices from the same number of mice. * treated group (corresponding color) versus control at the same time periods; + the maximum effect just before treatment versus the effects after (arrows in B , D , F , J ) in treated group. * or + P

Techniques Used: Mouse Assay

2) Product Images from "Biophotons Contribute to Retinal Dark Noise"

Article Title: Biophotons Contribute to Retinal Dark Noise

Journal: Neuroscience Bulletin

doi: 10.1007/s12264-016-0029-6

Effects of Ca 2+ and PDE on biophotonic activity in rat retina. A – D Removing intra- and extracellular Ca 2+ together ( A and B , 10 µmol/L BAPTA-AM + 0.5 mmol/L EGTA, n = 5) or introducing a
Figure Legend Snippet: Effects of Ca 2+ and PDE on biophotonic activity in rat retina. A – D Removing intra- and extracellular Ca 2+ together ( A and B , 10 µmol/L BAPTA-AM + 0.5 mmol/L EGTA, n = 5) or introducing a

Techniques Used: Activity Assay

3) Product Images from "Doxorubicin Induces the Persistent Activation of Intracellular Transglutaminase 2 That Protects from Cell Death"

Article Title: Doxorubicin Induces the Persistent Activation of Intracellular Transglutaminase 2 That Protects from Cell Death

Journal: Molecules and Cells

doi: 10.1007/s10059-012-2201-9

Doxorubicin-induced TG2 activation is inhibited by calcium chelators and caffeine. (A, B) The effect of BAPTA-AM (20 μM), EGTA (2 mM), or TLR2-neutralizing antibody (10 μg/ml) on TG activity after exposure to doxorubicin (1 μg/ml)
Figure Legend Snippet: Doxorubicin-induced TG2 activation is inhibited by calcium chelators and caffeine. (A, B) The effect of BAPTA-AM (20 μM), EGTA (2 mM), or TLR2-neutralizing antibody (10 μg/ml) on TG activity after exposure to doxorubicin (1 μg/ml)

Techniques Used: Activation Assay, Activity Assay

Related Articles

Isolation:

Article Title: PTENα regulates mitophagy and maintains mitochondrial quality control
Article Snippet: The heart slices were photographed with a digital camera, and the areas of infarction (white) were analyzed with ImageJ software as a percentage of total area. .. Mitochondria were isolated from mouse heart using an MSE buffer (220 mM mannitol [Amresco, 0122], 70 mM sucrose [Xilong Scientific, S3055], 2 mM EGTA, 5 mM MOPS, pH 7.4, 2 mM taurine [Amresco, 0599], 0.2% BSA). ..

Protease Inhibitor:

Article Title: Myosin-driven transport network in plants
Article Snippet: .. Rosette leaves from these plants were harvested and ground in 3 vol (wt/vol) of grinding buffer [20 mM Hepes (pH 7.2), 50 mM KOAc, 1 mM EDTA, 1 mM EGTA, 250 mM sorbitol, 2 mM DTT and 1× Plant Protease Inhibitor mixture (Amresco)] on ice. .. Homogenates were filtered through Miracloth (Calbiochem) and centrifuged at 3,000 × g for 10 min. Supernatants were loaded onto the 20% (wt/vol) sucrose cushion in the same buffer and centrifuged at 100,000 × g for 1 h. The 100,000 × g pellets containing microsomes were resuspended in the grinding buffer containing either 1 M NaCl or 1% Triton X-100.

Imaging:

Article Title: In vitro and in vivo roles of glucocorticoid and vitamin D receptors in the control of cardiomyocyte proliferative potential
Article Snippet: For all experiments, both male and female mice were used and no gender difference was observed. .. ReagentsThe following reagents were used: rabbit anti-PCM1 (1:2000) (Sigma HPA023370), rabbit anti-PCM1 (H262) (1:200) (Santa Cruz Biotechnology SC-67204), Alexa Fluor 488 donkey anti-rabbit IgG (Molecular Probes 1874771) (1:500), Rat anti Ki-67 monoclonal antibody (SolA15), Southern Biotech Dapi-Fluoromount-G Clear Mounting Media (Southern Biotech 0100-20), 5-Ethynyl-2-deoxyuridine (Santa Cruz Biotechnology SC-284628), Click-it EdU imaging kit (ThermoFisher Scientific C10337), Collagenase Type II (Worthington LS004177), Ethyl carbamate (Alfa Aesar AAA44804-18), calcitriol (Sigma-Aldrich 32222-06-3), alfacalcidol (Sigma-Aldrich L91201381), dexamethasone (TCI America TCI-D1961-1G), hydrocortisone (Alfa Aesar AAA16292-03), corticosterone (Enzo 89149-746), sodium chloride (Sigma-Aldrich S9888-10KG), potassium chloride (Sigma-Aldrich P9541-1KG), monopotassium phosphate (Fisher 5028048), sodium phosphate dibasic heptahydrate (Fisher S25837), magnesium sulfate heptahydrate (FisherS25414), HEPES (Sigma-Aldrich H4034-100g), sodium bicarbonate (EMD EM-SX0320-1), taurine (Sigma-Aldrich T8691-100G), biacetyl monoxime (Sigma-Aldrich B0753-100G), glucose (Amresco 97061-164), EGTA (Amresco 0732-288), protease XIV (P5147-100MG), fetal bovine serum (JRS CCFAP004), calcium chloride (Amresco 97061-904), primocin (Invivogen NC9141851), DMEM (CCF CCFAA005). .. Generating mouse lines Mice heterozygous for the Tg(Myh6-Cre)2182Mds transgene (here referred to as “Myh6-Cre ”) were bred with mice homozygous for the Nr3c1 f/f allele (here named Gr f/f ) to generate Myh6-Cre;Gr f /+ mice.

other:

Article Title: Biophotons Contribute to Retinal Dark Noise
Article Snippet: A phosphodiesterase 6 (PDE6) inhibitor (Zaprinast, 100 nmol/L, Sigma, St. Louis, MO), BAPTA-AM (10 μmol/L, Molecular Probes, Eugene, OR), and EGTA (0.5 mmol/L, Amresco, Solon, OH) were initially dissolved in DMSO and then diluted to their final concentrations in ACSF or Ringer’s solution.

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    Amresco egta
    Effects of Ca 2+ and PDE on biophotonic activity in rat retina. A – D Removing intra- and extracellular Ca 2+ together ( A and B , 10 µmol/L <t>BAPTA-AM</t> + 0.5 mmol/L <t>EGTA,</t> n = 5) or introducing a
    Egta, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta/product/Amresco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    egta - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Effects of Ca 2+ and PDE on biophotonic activity in rat retina. A – D Removing intra- and extracellular Ca 2+ together ( A and B , 10 µmol/L BAPTA-AM + 0.5 mmol/L EGTA, n = 5) or introducing a

    Journal: Neuroscience Bulletin

    Article Title: Biophotons Contribute to Retinal Dark Noise

    doi: 10.1007/s12264-016-0029-6

    Figure Lengend Snippet: Effects of Ca 2+ and PDE on biophotonic activity in rat retina. A – D Removing intra- and extracellular Ca 2+ together ( A and B , 10 µmol/L BAPTA-AM + 0.5 mmol/L EGTA, n = 5) or introducing a

    Article Snippet: A phosphodiesterase 6 (PDE6) inhibitor (Zaprinast, 100 nmol/L, Sigma, St. Louis, MO), BAPTA-AM (10 μmol/L, Molecular Probes, Eugene, OR), and EGTA (0.5 mmol/L, Amresco, Solon, OH) were initially dissolved in DMSO and then diluted to their final concentrations in ACSF or Ringer’s solution.

    Techniques: Activity Assay

    Doxorubicin-induced TG2 activation is inhibited by calcium chelators and caffeine. (A, B) The effect of BAPTA-AM (20 μM), EGTA (2 mM), or TLR2-neutralizing antibody (10 μg/ml) on TG activity after exposure to doxorubicin (1 μg/ml)

    Journal: Molecules and Cells

    Article Title: Doxorubicin Induces the Persistent Activation of Intracellular Transglutaminase 2 That Protects from Cell Death

    doi: 10.1007/s10059-012-2201-9

    Figure Lengend Snippet: Doxorubicin-induced TG2 activation is inhibited by calcium chelators and caffeine. (A, B) The effect of BAPTA-AM (20 μM), EGTA (2 mM), or TLR2-neutralizing antibody (10 μg/ml) on TG activity after exposure to doxorubicin (1 μg/ml)

    Article Snippet: To identify the mediators of doxorubicin-induced TG2 activation, the cells were pre-treated for 1 h with 1 mM of N-acetylcysteine (NAC; Sigma), 20 μM BAPTA-AM (Molecular probes), 2 mM EGTA (Amresco), 30 μg/ml transforming growth factor β (TGFβ)-neutralizing antibody (R & D Systems), 10 μg/ml toll-like receptor 2 (TLR2)-neutralizing antibody (R & D Systems) or 0–20 mM caffeine (Sigma) before the doxorubicin treatment.

    Techniques: Activation Assay, Activity Assay

    The effects of different treatments on the biophotonic activities in mouse coronal brain slices. ( A–D ) Removing extracellular Ca 2+ ( A and B , 0.5 mM EGTA, n = 6) or removing intra- and extracellular Ca 2+ together ( C and D , 10 µM BAPTA-AM+0.5 mM EGTA, n = 6) from the beginning of the application of 50 mM glutamate (pink line in A and C ). The changes were significant during the maintenance period ( B and D ). ( E, F ) 1 µM TTX had no influence on the initiation, but partly on the maintenance (pink line, n = 5). ( G, H ) Both initiation (green line, n = 5) and maintenance (pink line, n = 6) were significantly affected by 0.5% procaine. ( I, J ) Both initiation (green line, n = 6) and maintenance (pink line, n = 6) were significantly affected by 0.05% sodium azide (SA), but a recovery was found after long-lasting application. Arrows indicate the treatment at the beginning of the application of 50 mM glutamate in A , C , E , G and I and arrowheads mark the treatment that began after the achievement of the maximum effect in G and I . t: treated group; c: control group (blue line) is same in A–J (n = 6), i : the treatment together with application of glutamate (initiating period), m : the treatment during the maintenance period. Data show mean±s.e.m. n = the number of slices from the same number of mice. * treated group (corresponding color) versus control at the same time periods; + the maximum effect just before treatment versus the effects after (arrows in B , D , F , J ) in treated group. * or + P

    Journal: PLoS ONE

    Article Title: Spatiotemporal Imaging of Glutamate-Induced Biophotonic Activities and Transmission in Neural Circuits

    doi: 10.1371/journal.pone.0085643

    Figure Lengend Snippet: The effects of different treatments on the biophotonic activities in mouse coronal brain slices. ( A–D ) Removing extracellular Ca 2+ ( A and B , 0.5 mM EGTA, n = 6) or removing intra- and extracellular Ca 2+ together ( C and D , 10 µM BAPTA-AM+0.5 mM EGTA, n = 6) from the beginning of the application of 50 mM glutamate (pink line in A and C ). The changes were significant during the maintenance period ( B and D ). ( E, F ) 1 µM TTX had no influence on the initiation, but partly on the maintenance (pink line, n = 5). ( G, H ) Both initiation (green line, n = 5) and maintenance (pink line, n = 6) were significantly affected by 0.5% procaine. ( I, J ) Both initiation (green line, n = 6) and maintenance (pink line, n = 6) were significantly affected by 0.05% sodium azide (SA), but a recovery was found after long-lasting application. Arrows indicate the treatment at the beginning of the application of 50 mM glutamate in A , C , E , G and I and arrowheads mark the treatment that began after the achievement of the maximum effect in G and I . t: treated group; c: control group (blue line) is same in A–J (n = 6), i : the treatment together with application of glutamate (initiating period), m : the treatment during the maintenance period. Data show mean±s.e.m. n = the number of slices from the same number of mice. * treated group (corresponding color) versus control at the same time periods; + the maximum effect just before treatment versus the effects after (arrows in B , D , F , J ) in treated group. * or + P

    Article Snippet: BAPTA-AM (10 µM, Molecular Probes, Eugene, OR, USA), EGTA (0.5 mM, Amresco, Solon, OH, USA) and okadaic acid potassium salt (OA, 200 nM, LC Lab, Woburn, MA, USA) were initially dissolved in DMSO and then diluted to their final concentration in the ACSF.

    Techniques: Mouse Assay