edta solution  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Ethylenediaminetetraacetic acid solution
    Description:
    Ethylenediaminetetraacetic acid EDTA is an aminopolycarboxylic acid and a hexadentate ligand It chelates with metal ions to form an octahedral complex especially with cations Ethylenediaminetetraacetic acid EDTA is an anticoagulant which prevents clotting of blood It is implicated in platelet aggregation in pseudothrombocytopenia EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease It is a chelator used to remove calcium from cell washing and suspension media EDTA reduces cell clumping
    Catalog Number:
    e8008
    Price:
    None
    Applications:
    Ethylenediaminetetraacetic acid solution has been used to remove confluent cell monolayers in swine testicular (ST-cells) culture. It has also been used to detach embryonic stem cells (ESCs) from matrigel and primary human fibroblasts.
    Buy from Supplier


    Structured Review

    Millipore edta solution
    Ethylenediaminetetraacetic acid solution
    Ethylenediaminetetraacetic acid EDTA is an aminopolycarboxylic acid and a hexadentate ligand It chelates with metal ions to form an octahedral complex especially with cations Ethylenediaminetetraacetic acid EDTA is an anticoagulant which prevents clotting of blood It is implicated in platelet aggregation in pseudothrombocytopenia EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease It is a chelator used to remove calcium from cell washing and suspension media EDTA reduces cell clumping
    https://www.bioz.com/result/edta solution/product/Millipore
    Average 99 stars, based on 56 article reviews
    Price from $9.99 to $1999.99
    edta solution - by Bioz Stars, 2020-04
    99/100 stars

    Images

    1) Product Images from "An electrochemical immunosensing method for detecting melanoma cells"

    Article Title: An electrochemical immunosensing method for detecting melanoma cells

    Journal: Biosensors & bioelectronics

    doi: 10.1016/j.bios.2015.01.022

    Schematic of (A) fabrication of the immunosensor and (B) reaction occurring at the working electrode surface in 1 × PBS solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM EDTA at scan rate 50 mV s −1 .
    Figure Legend Snippet: Schematic of (A) fabrication of the immunosensor and (B) reaction occurring at the working electrode surface in 1 × PBS solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM EDTA at scan rate 50 mV s −1 .

    Techniques Used:

    (A) Typical cyclic voltammograms of (a) bare SPE (b) PPy/SPE, (c) n-SiNPs/PPy/SPE and (d) MC1R-Ab/n-SiNPs/PPy/SPE in 1 × PBS solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM EDTA at scan rate 50 mV s −1
    Figure Legend Snippet: (A) Typical cyclic voltammograms of (a) bare SPE (b) PPy/SPE, (c) n-SiNPs/PPy/SPE and (d) MC1R-Ab/n-SiNPs/PPy/SPE in 1 × PBS solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM EDTA at scan rate 50 mV s −1

    Techniques Used:

    Related Articles

    Centrifugation:

    Article Title: The Challenge of Producing Skin Test Antigens with Minimal Resources Suitable for Human Application against a Neglected Tropical Disease; LeprosySafety and Efficacy Assessment of Two New Leprosy Skin Test Antigens: Randomized Double Blind Clinical Study
    Article Snippet: Tissue sections ranging from 19 g to 36.5 g were homogenized with 10 mM disodium ethylenediaminetetraacetic acid (EDTA, Sigma, St. Louis, Mo.), pH 8.0 at 3 ml/g of tissue. .. Tissue fragments were pelleted and washed twice with 10 mM EDTA by centrifugation (Sorvall RC5, Thermo Fisher Scientific, Inc., Rockford, IL) at 15,000× g for 10 min at 4°C in 50 ml Teflon Oakridge tubes, followed by extraction with 0.1 M sodium hydroxide (Mallinckrodt Baker Inc., Phillipsburg, NJ) in 10 mM EDTA while stirring at room temperature for 2 h to remove pigment and to separate M. leprae from tissue.

    Article Title: Murine Norovirus: Propagation, Quantification and Genetic Manipulation
    Article Snippet: .. Day 4 bone marrow culture for the derivation of macrophages (Support Protocol 5) Lentiviral vectors (Support Protocol 6) PBS/EDTA solution (e.g., Sigma, cat no. E8008) Sterile cell lifter (e.g., Fisher Scientific, cat. no. 08 100 240) Macrophage complete medium (see recipe) Additional reagents and equipment for cell counting (e.g., hemacytometer), centrifugation and cell culture (e.g., biosafety cabinet and 37°C/5% CO2 incubator) On Day 4 of bone marrow culture (Support Protocol 5), gently swirl the plate a couple of times to detach any loosely bound materials and remove the unbound with culture medium. ..

    Synthesized:

    Article Title: A heparin-modified thermoresponsive surface with heparin-binding epidermal growth factor-like growth factor for maintaining hepatic functions in vitro and harvesting hepatocyte sheets
    Article Snippet: 2-Carboxyisopropylacrylamide (CIPAAm) was synthesized using the method described previously . .. Heparin sodium salt from porcine intestinal mucosa (grade I-A, 180 USP units/mg), Dulbecco's modified Eagle's medium (DMEM), Dulbecco's phosphate buffered saline (PBS), and trypsin/ethylenediaminetetraacetic acid (EDTA) solution were obtained from Sigma–Aldrich (St. Louis, MO, USA).

    Cytometry:

    Article Title: Anticancer activity of paclitaxel-loaded keratin nanoparticles in two-dimensional and perfused three-dimensional breast cancer models
    Article Snippet: PTX labeled with a thiophene-based fluorescent dye (PTX-F35) cellular uptake was carried out on C6 flow cytometry equipment (Accuri Cytometers, Milan, Italy). .. Briefly 8×104 cells were plated in a six-well culture plate (TPP, Trasadingen, Switzerland) and incubated with PTX, PTX-F35, and PTX-F35 and loaded in KER-NPs (KER-NPs-PTX-F35) ([PTX-F35] =5 µg/mL) for 2, 4, 6, and 24 h. Cells were detached after each incubation period using a 0.05% trypsin–0.02% EDTA solution (Sigma-Aldrich, Co., St Louis, MO, USA) and re-suspended in 300 µL of PBS (Sigma-Aldrich, Co.).

    Blocking Assay:

    Article Title: Anatomically-specific intratubular and interstitial biominerals in the human renal medullo-papillary complex
    Article Snippet: .. The ultrasections were decalcified with 4% EDTA solution for 20 min. Decalcified sections were washed twice using Milli-Q water, twice with PBS, and were treated with a blocking agent containing 2.5% bovine serum albumin (BSA, Sigma-Aldrich) in PBS for 10 min. Grids were incubated in primary antibody solution (1:20 dilution in PBS) at 4°C overnight. .. After washing three times with PBS, sections were treated again with 2.5% BSA for 10 min then incubated in a 10-nm-diameter protein G-gold nanoparticle solution (Electron Microscopy Sciences, Hatfield, PA) for 1 hr at room temperature.

    Incubation:

    Article Title: Anticancer activity of paclitaxel-loaded keratin nanoparticles in two-dimensional and perfused three-dimensional breast cancer models
    Article Snippet: .. Briefly 8×104 cells were plated in a six-well culture plate (TPP, Trasadingen, Switzerland) and incubated with PTX, PTX-F35, and PTX-F35 and loaded in KER-NPs (KER-NPs-PTX-F35) ([PTX-F35] =5 µg/mL) for 2, 4, 6, and 24 h. Cells were detached after each incubation period using a 0.05% trypsin–0.02% EDTA solution (Sigma-Aldrich, Co., St Louis, MO, USA) and re-suspended in 300 µL of PBS (Sigma-Aldrich, Co.). .. They were then run on the C6 flow cytometer (Accuri Cytometers), which considered 10,000 events, using 488 nm excitation to measure the intracellular PTX-F35.

    Article Title: Anatomically-specific intratubular and interstitial biominerals in the human renal medullo-papillary complex
    Article Snippet: .. The ultrasections were decalcified with 4% EDTA solution for 20 min. Decalcified sections were washed twice using Milli-Q water, twice with PBS, and were treated with a blocking agent containing 2.5% bovine serum albumin (BSA, Sigma-Aldrich) in PBS for 10 min. Grids were incubated in primary antibody solution (1:20 dilution in PBS) at 4°C overnight. .. After washing three times with PBS, sections were treated again with 2.5% BSA for 10 min then incubated in a 10-nm-diameter protein G-gold nanoparticle solution (Electron Microscopy Sciences, Hatfield, PA) for 1 hr at room temperature.

    Article Title: Anti-malarial Activities of Two Soil Actinomycete Isolates from Sabah via Inhibition of Glycogen Synthase Kinase 3β
    Article Snippet: On day 4 post-infection, liver organs were harvested, homogenised and proteins were extracted using a protein extraction buffer containing 50 mM TrisHCl (Sigma Aldrich, Missouri, USA), 150 mM NaCl, 1% Triton X-100 (Merck, New Jersey, USA), phosphatase and protease inhibitors (1 mM ethylenediaminetetraacetic acid [EDTA], 1 mM ethylene glycol tetraacetic acid [EGTA], 0.5 mM Na3 VO4 , 0.5 mM phenylmethylsulfonyl fluoride [PMSF], 1 μg/mL aprotinin, 5 μg/mL leupeptin and 1 mM NaF; all procured from Sigma Aldrich, Missouri, USA) ( ). .. Following gel electrophoresis, proteins were electro-transferred onto nitrocellulose membranes using Novex semi-dry blotter (Invitrogen, California) and the membranes were blocked with 3% BSA for 1 h before overnight incubation with primary monoclonal antibodies; anti-GSK3β (Cell Signaling Technology, Danvers, Massachusetts, USA) or anti-phospho Ser 9-GSK3β (Calbiochem, Seattle, Washington, USA).

    Cell Culture:

    Article Title: An electrochemical immunosensing method for detecting melanoma cells
    Article Snippet: Paragraph title: 2.1. Cell culture and cell suspension preparation ... Cells were dislodged from the surface of the culture dish by using 0.25% Trypsin–EDTA (Life Technologies, Grand Island, NY), or 2% EDTA solution in 1 × PBS (Sigma, St. Louis, MO), or a cell scraper (Sarstedt, Newton, NC).

    Article Title: Murine Norovirus: Propagation, Quantification and Genetic Manipulation
    Article Snippet: .. Day 4 bone marrow culture for the derivation of macrophages (Support Protocol 5) Lentiviral vectors (Support Protocol 6) PBS/EDTA solution (e.g., Sigma, cat no. E8008) Sterile cell lifter (e.g., Fisher Scientific, cat. no. 08 100 240) Macrophage complete medium (see recipe) Additional reagents and equipment for cell counting (e.g., hemacytometer), centrifugation and cell culture (e.g., biosafety cabinet and 37°C/5% CO2 incubator) On Day 4 of bone marrow culture (Support Protocol 5), gently swirl the plate a couple of times to detach any loosely bound materials and remove the unbound with culture medium. ..

    Article Title: The Concomitant Expression of Human Endogenous Retroviruses and Embryonic Genes in Cancer Cells under Microenvironmental Changes is a Potential Target for Antiretroviral Drugs
    Article Snippet: The TVM-A12 cell line was cultured in RPMI-1640 medium, while HepG2 and A549 cell lines were cultured in DMEM medium. .. All cell lines were maintained at 37 °C in a humidified 5% CO2 atmosphere and passaged twice weekly after detachment with trypsin (0.05%) and EDTA solution (0.02%) in PBS (Sigma).

    Modification:

    Article Title: The Challenge of Producing Skin Test Antigens with Minimal Resources Suitable for Human Application against a Neglected Tropical Disease; LeprosySafety and Efficacy Assessment of Two New Leprosy Skin Test Antigens: Randomized Double Blind Clinical Study
    Article Snippet: A563 (19 g spleen, one preparation), A572 (109 g liver, divided into three preparations), and A581 (114 g liver, divided into three preparations)] were fractionated using a modified 3/77 Draper protocol , except for omission of the step involving protease digestion with chymotrypsin and trypsin and alterations in buffer composition. .. Tissue sections ranging from 19 g to 36.5 g were homogenized with 10 mM disodium ethylenediaminetetraacetic acid (EDTA, Sigma, St. Louis, Mo.), pH 8.0 at 3 ml/g of tissue.

    Article Title: A heparin-modified thermoresponsive surface with heparin-binding epidermal growth factor-like growth factor for maintaining hepatic functions in vitro and harvesting hepatocyte sheets
    Article Snippet: .. Heparin sodium salt from porcine intestinal mucosa (grade I-A, 180 USP units/mg), Dulbecco's modified Eagle's medium (DMEM), Dulbecco's phosphate buffered saline (PBS), and trypsin/ethylenediaminetetraacetic acid (EDTA) solution were obtained from Sigma–Aldrich (St. Louis, MO, USA). .. Heparin-binding epidermal growth factor-like growth factor (HB-EGF) was purchased from R & D Systems (Minneapolis, Minnesota, USA). l -Proline was obtained from ICN Biomedicals (Aurora, OH, USA).

    Western Blot:

    Article Title: Anti-malarial Activities of Two Soil Actinomycete Isolates from Sabah via Inhibition of Glycogen Synthase Kinase 3β
    Article Snippet: Paragraph title: Western Analysis ... On day 4 post-infection, liver organs were harvested, homogenised and proteins were extracted using a protein extraction buffer containing 50 mM TrisHCl (Sigma Aldrich, Missouri, USA), 150 mM NaCl, 1% Triton X-100 (Merck, New Jersey, USA), phosphatase and protease inhibitors (1 mM ethylenediaminetetraacetic acid [EDTA], 1 mM ethylene glycol tetraacetic acid [EGTA], 0.5 mM Na3 VO4 , 0.5 mM phenylmethylsulfonyl fluoride [PMSF], 1 μg/mL aprotinin, 5 μg/mL leupeptin and 1 mM NaF; all procured from Sigma Aldrich, Missouri, USA) ( ).

    Recrystallization:

    Article Title: A heparin-modified thermoresponsive surface with heparin-binding epidermal growth factor-like growth factor for maintaining hepatic functions in vitro and harvesting hepatocyte sheets
    Article Snippet: 2.1 Chemicals and materials N -Isopropylacrylamide (IPAAm) was obtained from Kohjin (Tokyo, Japan) and purified by recrystallization from n -hexane. .. Heparin sodium salt from porcine intestinal mucosa (grade I-A, 180 USP units/mg), Dulbecco's modified Eagle's medium (DMEM), Dulbecco's phosphate buffered saline (PBS), and trypsin/ethylenediaminetetraacetic acid (EDTA) solution were obtained from Sigma–Aldrich (St. Louis, MO, USA).

    Sterility:

    Article Title: The Challenge of Producing Skin Test Antigens with Minimal Resources Suitable for Human Application against a Neglected Tropical Disease; LeprosySafety and Efficacy Assessment of Two New Leprosy Skin Test Antigens: Randomized Double Blind Clinical Study
    Article Snippet: Tissue sections ranging from 19 g to 36.5 g were homogenized with 10 mM disodium ethylenediaminetetraacetic acid (EDTA, Sigma, St. Louis, Mo.), pH 8.0 at 3 ml/g of tissue. .. Homogenates were tested for sterility on brain heart infusion agar, blood agar, and Lowenstein-Jensen agar (BD, Franklin Lakes, NJ).

    Electron Microscopy:

    Article Title: Anatomically-specific intratubular and interstitial biominerals in the human renal medullo-papillary complex
    Article Snippet: Paragraph title: Polarized and fluorescent microscopy techniques, and immunohistochemistry on tissue sections of the medullo-papillary complex for noncollagenous protein localization using light and transmission electron microscopy techniques ... The ultrasections were decalcified with 4% EDTA solution for 20 min. Decalcified sections were washed twice using Milli-Q water, twice with PBS, and were treated with a blocking agent containing 2.5% bovine serum albumin (BSA, Sigma-Aldrich) in PBS for 10 min. Grids were incubated in primary antibody solution (1:20 dilution in PBS) at 4°C overnight.

    Immunohistochemistry:

    Article Title: Anatomically-specific intratubular and interstitial biominerals in the human renal medullo-papillary complex
    Article Snippet: Paragraph title: Polarized and fluorescent microscopy techniques, and immunohistochemistry on tissue sections of the medullo-papillary complex for noncollagenous protein localization using light and transmission electron microscopy techniques ... The ultrasections were decalcified with 4% EDTA solution for 20 min. Decalcified sections were washed twice using Milli-Q water, twice with PBS, and were treated with a blocking agent containing 2.5% bovine serum albumin (BSA, Sigma-Aldrich) in PBS for 10 min. Grids were incubated in primary antibody solution (1:20 dilution in PBS) at 4°C overnight.

    Infection:

    Article Title: The Challenge of Producing Skin Test Antigens with Minimal Resources Suitable for Human Application against a Neglected Tropical Disease; LeprosySafety and Efficacy Assessment of Two New Leprosy Skin Test Antigens: Randomized Double Blind Clinical Study
    Article Snippet: Tissue Fractionation A total of 242 g of M. leprae infected tissue (spleen, 19 g; liver, 223 g) from three infected armadillos [animal nos. .. Tissue sections ranging from 19 g to 36.5 g were homogenized with 10 mM disodium ethylenediaminetetraacetic acid (EDTA, Sigma, St. Louis, Mo.), pH 8.0 at 3 ml/g of tissue.

    Article Title: Anti-malarial Activities of Two Soil Actinomycete Isolates from Sabah via Inhibition of Glycogen Synthase Kinase 3β
    Article Snippet: To determine the phosphorylation state of GSK3β in samples, infected mice were administered with effective (chemo-suppressive) dosages of each test sample, lithium chloride or chloroquine for four consecutive days. .. On day 4 post-infection, liver organs were harvested, homogenised and proteins were extracted using a protein extraction buffer containing 50 mM TrisHCl (Sigma Aldrich, Missouri, USA), 150 mM NaCl, 1% Triton X-100 (Merck, New Jersey, USA), phosphatase and protease inhibitors (1 mM ethylenediaminetetraacetic acid [EDTA], 1 mM ethylene glycol tetraacetic acid [EGTA], 0.5 mM Na3 VO4 , 0.5 mM phenylmethylsulfonyl fluoride [PMSF], 1 μg/mL aprotinin, 5 μg/mL leupeptin and 1 mM NaF; all procured from Sigma Aldrich, Missouri, USA) ( ).

    Light Microscopy:

    Article Title: Anatomically-specific intratubular and interstitial biominerals in the human renal medullo-papillary complex
    Article Snippet: The ultrasections were decalcified with 4% EDTA solution for 20 min. Decalcified sections were washed twice using Milli-Q water, twice with PBS, and were treated with a blocking agent containing 2.5% bovine serum albumin (BSA, Sigma-Aldrich) in PBS for 10 min. Grids were incubated in primary antibody solution (1:20 dilution in PBS) at 4°C overnight. .. The ultrasections were decalcified with 4% EDTA solution for 20 min. Decalcified sections were washed twice using Milli-Q water, twice with PBS, and were treated with a blocking agent containing 2.5% bovine serum albumin (BSA, Sigma-Aldrich) in PBS for 10 min. Grids were incubated in primary antibody solution (1:20 dilution in PBS) at 4°C overnight.

    other:

    Article Title: Etidronate from Medicine to Endodontics: effects of different irrigation regimes on root dentin roughness
    Article Snippet: The 17% EDTA solution (Sigma Aldrich) was prepared as previously described in other study .

    Transmission Assay:

    Article Title: Anatomically-specific intratubular and interstitial biominerals in the human renal medullo-papillary complex
    Article Snippet: Paragraph title: Polarized and fluorescent microscopy techniques, and immunohistochemistry on tissue sections of the medullo-papillary complex for noncollagenous protein localization using light and transmission electron microscopy techniques ... The ultrasections were decalcified with 4% EDTA solution for 20 min. Decalcified sections were washed twice using Milli-Q water, twice with PBS, and were treated with a blocking agent containing 2.5% bovine serum albumin (BSA, Sigma-Aldrich) in PBS for 10 min. Grids were incubated in primary antibody solution (1:20 dilution in PBS) at 4°C overnight.

    Injection:

    Article Title: Development of a blood oxygenation phantom for photoacoustic tomography combined with online pO2 detection and flow spectrometry
    Article Snippet: Prior to the experiment, ethylenediaminetetraacetic acid anticoagulant (9002-07-7, Sigma-Aldrich) was added to the fresh blood, which was kept in a refrigerator under 4°C for no more than 72 hours. .. The sodium hydrosulfite was dissolved in phosphate-buffered saline (PBS) ∼ 0.03 % w / v for injection into the flow circuit.

    Nucleic Acid Electrophoresis:

    Article Title: Anti-malarial Activities of Two Soil Actinomycete Isolates from Sabah via Inhibition of Glycogen Synthase Kinase 3β
    Article Snippet: On day 4 post-infection, liver organs were harvested, homogenised and proteins were extracted using a protein extraction buffer containing 50 mM TrisHCl (Sigma Aldrich, Missouri, USA), 150 mM NaCl, 1% Triton X-100 (Merck, New Jersey, USA), phosphatase and protease inhibitors (1 mM ethylenediaminetetraacetic acid [EDTA], 1 mM ethylene glycol tetraacetic acid [EGTA], 0.5 mM Na3 VO4 , 0.5 mM phenylmethylsulfonyl fluoride [PMSF], 1 μg/mL aprotinin, 5 μg/mL leupeptin and 1 mM NaF; all procured from Sigma Aldrich, Missouri, USA) ( ). .. Protein samples were separated using 12% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) ( ).

    Fluorescence:

    Article Title: Anticancer activity of paclitaxel-loaded keratin nanoparticles in two-dimensional and perfused three-dimensional breast cancer models
    Article Snippet: Briefly 8×104 cells were plated in a six-well culture plate (TPP, Trasadingen, Switzerland) and incubated with PTX, PTX-F35, and PTX-F35 and loaded in KER-NPs (KER-NPs-PTX-F35) ([PTX-F35] =5 µg/mL) for 2, 4, 6, and 24 h. Cells were detached after each incubation period using a 0.05% trypsin–0.02% EDTA solution (Sigma-Aldrich, Co., St Louis, MO, USA) and re-suspended in 300 µL of PBS (Sigma-Aldrich, Co.). .. Intracellular fluorescence is expressed as integrated median fluorescence intensity (iMFI) ratio.

    Article Title: Effects of Eleutherococcus Extract Mixture on Endochondral Bone Formation in Rats
    Article Snippet: The dissected tibias were fixed with 4% para-formaldehyde, and then decalcificated in 50 mM ethylene diaminetetra acetic acid solution (Sigma). .. The daily rate of endochondral bone formation was calculated by measuring the length between the epiphyseal end line of the GP and the proximal endpoint of the fluorescence line, and dividing the distance by 3.

    Article Title: Effects of Huang Bai (Phellodendri Cortex) on bone growth and pubertal development in adolescent female rats
    Article Snippet: Fixed tibias were decalcified by immersion in 50 mM ethylene diamine tetra acetic acid solution (Sigma-Aldrich) for 2 d. Decalcified bones were dehydrated by immersion in 30% sucrose (Sigma-Aldrich) for 1 day. .. Fixed tibias were decalcified by immersion in 50 mM ethylene diamine tetra acetic acid solution (Sigma-Aldrich) for 2 d. Decalcified bones were dehydrated by immersion in 30% sucrose (Sigma-Aldrich) for 1 day.

    Multiple Displacement Amplification:

    Article Title: Anticancer activity of paclitaxel-loaded keratin nanoparticles in two-dimensional and perfused three-dimensional breast cancer models
    Article Snippet: Evaluation of cytotoxicity of albumin and KER-NPs In order to evaluate if our delivery systems, ie, albumin nanoparticles (HSA-NPs) and keratin nanoparticles (KER-NPs), were safe as delivery NPs, MCF-7 and MDA MB 231 cells were incubated with increasing concentrations of compounds. .. Briefly 8×104 cells were plated in a six-well culture plate (TPP, Trasadingen, Switzerland) and incubated with PTX, PTX-F35, and PTX-F35 and loaded in KER-NPs (KER-NPs-PTX-F35) ([PTX-F35] =5 µg/mL) for 2, 4, 6, and 24 h. Cells were detached after each incubation period using a 0.05% trypsin–0.02% EDTA solution (Sigma-Aldrich, Co., St Louis, MO, USA) and re-suspended in 300 µL of PBS (Sigma-Aldrich, Co.).

    Flow Cytometry:

    Article Title: Anticancer activity of paclitaxel-loaded keratin nanoparticles in two-dimensional and perfused three-dimensional breast cancer models
    Article Snippet: PTX labeled with a thiophene-based fluorescent dye (PTX-F35) cellular uptake was carried out on C6 flow cytometry equipment (Accuri Cytometers, Milan, Italy). .. Briefly 8×104 cells were plated in a six-well culture plate (TPP, Trasadingen, Switzerland) and incubated with PTX, PTX-F35, and PTX-F35 and loaded in KER-NPs (KER-NPs-PTX-F35) ([PTX-F35] =5 µg/mL) for 2, 4, 6, and 24 h. Cells were detached after each incubation period using a 0.05% trypsin–0.02% EDTA solution (Sigma-Aldrich, Co., St Louis, MO, USA) and re-suspended in 300 µL of PBS (Sigma-Aldrich, Co.).

    Microscopy:

    Article Title: Effects of Eleutherococcus Extract Mixture on Endochondral Bone Formation in Rats
    Article Snippet: The dissected tibias were fixed with 4% para-formaldehyde, and then decalcificated in 50 mM ethylene diaminetetra acetic acid solution (Sigma). .. Fluorescence line was observed by a fluorescence microscope (BX50, Olympus, Tokyo, Japan) and the distance was calculated with Image J (NIH, Bethesda, MD, USA).

    Article Title: Anatomically-specific intratubular and interstitial biominerals in the human renal medullo-papillary complex
    Article Snippet: Paragraph title: Polarized and fluorescent microscopy techniques, and immunohistochemistry on tissue sections of the medullo-papillary complex for noncollagenous protein localization using light and transmission electron microscopy techniques ... The ultrasections were decalcified with 4% EDTA solution for 20 min. Decalcified sections were washed twice using Milli-Q water, twice with PBS, and were treated with a blocking agent containing 2.5% bovine serum albumin (BSA, Sigma-Aldrich) in PBS for 10 min. Grids were incubated in primary antibody solution (1:20 dilution in PBS) at 4°C overnight.

    Article Title: Effects of Huang Bai (Phellodendri Cortex) on bone growth and pubertal development in adolescent female rats
    Article Snippet: Fixed tibias were decalcified by immersion in 50 mM ethylene diamine tetra acetic acid solution (Sigma-Aldrich) for 2 d. Decalcified bones were dehydrated by immersion in 30% sucrose (Sigma-Aldrich) for 1 day. .. Fixed tibias were decalcified by immersion in 50 mM ethylene diamine tetra acetic acid solution (Sigma-Aldrich) for 2 d. Decalcified bones were dehydrated by immersion in 30% sucrose (Sigma-Aldrich) for 1 day.

    Mouse Assay:

    Article Title: Anti-malarial Activities of Two Soil Actinomycete Isolates from Sabah via Inhibition of Glycogen Synthase Kinase 3β
    Article Snippet: To determine the phosphorylation state of GSK3β in samples, infected mice were administered with effective (chemo-suppressive) dosages of each test sample, lithium chloride or chloroquine for four consecutive days. .. On day 4 post-infection, liver organs were harvested, homogenised and proteins were extracted using a protein extraction buffer containing 50 mM TrisHCl (Sigma Aldrich, Missouri, USA), 150 mM NaCl, 1% Triton X-100 (Merck, New Jersey, USA), phosphatase and protease inhibitors (1 mM ethylenediaminetetraacetic acid [EDTA], 1 mM ethylene glycol tetraacetic acid [EGTA], 0.5 mM Na3 VO4 , 0.5 mM phenylmethylsulfonyl fluoride [PMSF], 1 μg/mL aprotinin, 5 μg/mL leupeptin and 1 mM NaF; all procured from Sigma Aldrich, Missouri, USA) ( ).

    Protein Extraction:

    Article Title: Anti-malarial Activities of Two Soil Actinomycete Isolates from Sabah via Inhibition of Glycogen Synthase Kinase 3β
    Article Snippet: .. On day 4 post-infection, liver organs were harvested, homogenised and proteins were extracted using a protein extraction buffer containing 50 mM TrisHCl (Sigma Aldrich, Missouri, USA), 150 mM NaCl, 1% Triton X-100 (Merck, New Jersey, USA), phosphatase and protease inhibitors (1 mM ethylenediaminetetraacetic acid [EDTA], 1 mM ethylene glycol tetraacetic acid [EGTA], 0.5 mM Na3 VO4 , 0.5 mM phenylmethylsulfonyl fluoride [PMSF], 1 μg/mL aprotinin, 5 μg/mL leupeptin and 1 mM NaF; all procured from Sigma Aldrich, Missouri, USA) ( ). .. Protein concentrations were measured using the Bradford method described by with bovine serum albumin (BSA, Sigma Aldrich, Missouri, USA) as a standard.

    Labeling:

    Article Title: Anticancer activity of paclitaxel-loaded keratin nanoparticles in two-dimensional and perfused three-dimensional breast cancer models
    Article Snippet: PTX labeled with a thiophene-based fluorescent dye (PTX-F35) cellular uptake was carried out on C6 flow cytometry equipment (Accuri Cytometers, Milan, Italy). .. Briefly 8×104 cells were plated in a six-well culture plate (TPP, Trasadingen, Switzerland) and incubated with PTX, PTX-F35, and PTX-F35 and loaded in KER-NPs (KER-NPs-PTX-F35) ([PTX-F35] =5 µg/mL) for 2, 4, 6, and 24 h. Cells were detached after each incubation period using a 0.05% trypsin–0.02% EDTA solution (Sigma-Aldrich, Co., St Louis, MO, USA) and re-suspended in 300 µL of PBS (Sigma-Aldrich, Co.).

    Article Title: Anatomically-specific intratubular and interstitial biominerals in the human renal medullo-papillary complex
    Article Snippet: Immunolocalization of NCPs was performed through immunogold labeling of thin sections on nickel grids using polyclonal antibodies [ ]. .. The ultrasections were decalcified with 4% EDTA solution for 20 min. Decalcified sections were washed twice using Milli-Q water, twice with PBS, and were treated with a blocking agent containing 2.5% bovine serum albumin (BSA, Sigma-Aldrich) in PBS for 10 min. Grids were incubated in primary antibody solution (1:20 dilution in PBS) at 4°C overnight.

    Purification:

    Article Title: A heparin-modified thermoresponsive surface with heparin-binding epidermal growth factor-like growth factor for maintaining hepatic functions in vitro and harvesting hepatocyte sheets
    Article Snippet: 2.1 Chemicals and materials N -Isopropylacrylamide (IPAAm) was obtained from Kohjin (Tokyo, Japan) and purified by recrystallization from n -hexane. .. Heparin sodium salt from porcine intestinal mucosa (grade I-A, 180 USP units/mg), Dulbecco's modified Eagle's medium (DMEM), Dulbecco's phosphate buffered saline (PBS), and trypsin/ethylenediaminetetraacetic acid (EDTA) solution were obtained from Sigma–Aldrich (St. Louis, MO, USA).

    SDS Page:

    Article Title: Anti-malarial Activities of Two Soil Actinomycete Isolates from Sabah via Inhibition of Glycogen Synthase Kinase 3β
    Article Snippet: On day 4 post-infection, liver organs were harvested, homogenised and proteins were extracted using a protein extraction buffer containing 50 mM TrisHCl (Sigma Aldrich, Missouri, USA), 150 mM NaCl, 1% Triton X-100 (Merck, New Jersey, USA), phosphatase and protease inhibitors (1 mM ethylenediaminetetraacetic acid [EDTA], 1 mM ethylene glycol tetraacetic acid [EGTA], 0.5 mM Na3 VO4 , 0.5 mM phenylmethylsulfonyl fluoride [PMSF], 1 μg/mL aprotinin, 5 μg/mL leupeptin and 1 mM NaF; all procured from Sigma Aldrich, Missouri, USA) ( ). .. Protein samples were separated using 12% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) ( ).

    Software:

    Article Title: Effects of Huang Bai (Phellodendri Cortex) on bone growth and pubertal development in adolescent female rats
    Article Snippet: Fixed tibias were decalcified by immersion in 50 mM ethylene diamine tetra acetic acid solution (Sigma-Aldrich) for 2 d. Decalcified bones were dehydrated by immersion in 30% sucrose (Sigma-Aldrich) for 1 day. .. The longitudinal bone length between the fluorescent line formed by tetracycline and the epiphyseal end line of the growth plate was measured by two blinded investigators (SHL and YSK) using Image J software (National Institutes of Health; MD, USA).

    Cell Attachment Assay:

    Article Title: The Concomitant Expression of Human Endogenous Retroviruses and Embryonic Genes in Cancer Cells under Microenvironmental Changes is a Potential Target for Antiretroviral Drugs
    Article Snippet: All cell lines were maintained at 37 °C in a humidified 5% CO2 atmosphere and passaged twice weekly after detachment with trypsin (0.05%) and EDTA solution (0.02%) in PBS (Sigma). .. Specifically, cells were cultured for 18 h in SM to ensure cell attachment; afterward, cell were washed with PBS and cultivated in SM or X-VIVO for 72 h. Cells were than detached and back cultured with the same fresh media for further 24 h.

    Cell Counting:

    Article Title: Murine Norovirus: Propagation, Quantification and Genetic Manipulation
    Article Snippet: .. Day 4 bone marrow culture for the derivation of macrophages (Support Protocol 5) Lentiviral vectors (Support Protocol 6) PBS/EDTA solution (e.g., Sigma, cat no. E8008) Sterile cell lifter (e.g., Fisher Scientific, cat. no. 08 100 240) Macrophage complete medium (see recipe) Additional reagents and equipment for cell counting (e.g., hemacytometer), centrifugation and cell culture (e.g., biosafety cabinet and 37°C/5% CO2 incubator) On Day 4 of bone marrow culture (Support Protocol 5), gently swirl the plate a couple of times to detach any loosely bound materials and remove the unbound with culture medium. ..

    Fractionation:

    Article Title: The Challenge of Producing Skin Test Antigens with Minimal Resources Suitable for Human Application against a Neglected Tropical Disease; LeprosySafety and Efficacy Assessment of Two New Leprosy Skin Test Antigens: Randomized Double Blind Clinical Study
    Article Snippet: Paragraph title: Tissue Fractionation ... Tissue sections ranging from 19 g to 36.5 g were homogenized with 10 mM disodium ethylenediaminetetraacetic acid (EDTA, Sigma, St. Louis, Mo.), pH 8.0 at 3 ml/g of tissue.

    Staining:

    Article Title: Anatomically-specific intratubular and interstitial biominerals in the human renal medullo-papillary complex
    Article Snippet: Afterwards, the sections were stained using secondary antibody kits (Vectastain Elite ABC, Vector Laboratories, Inc., Burlingame, CA) and DAB substrate kit (ImmPACT DAB peroxidase substrate, Vector Laboratories, Inc.). .. The ultrasections were decalcified with 4% EDTA solution for 20 min. Decalcified sections were washed twice using Milli-Q water, twice with PBS, and were treated with a blocking agent containing 2.5% bovine serum albumin (BSA, Sigma-Aldrich) in PBS for 10 min. Grids were incubated in primary antibody solution (1:20 dilution in PBS) at 4°C overnight.

    Hood:

    Article Title: Murine Norovirus: Propagation, Quantification and Genetic Manipulation
    Article Snippet: .. Day 4 bone marrow culture for the derivation of macrophages (Support Protocol 5) Lentiviral vectors (Support Protocol 6) PBS/EDTA solution (e.g., Sigma, cat no. E8008) Sterile cell lifter (e.g., Fisher Scientific, cat. no. 08 100 240) Macrophage complete medium (see recipe) Additional reagents and equipment for cell counting (e.g., hemacytometer), centrifugation and cell culture (e.g., biosafety cabinet and 37°C/5% CO2 incubator) On Day 4 of bone marrow culture (Support Protocol 5), gently swirl the plate a couple of times to detach any loosely bound materials and remove the unbound with culture medium. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore tris edta te buffer
    Tris Edta Te Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris edta te buffer/product/Millipore
    Average 99 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    tris edta te buffer - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    86
    Millipore phosphate buffered edta solution
    Phosphate Buffered Edta Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered edta solution/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered edta solution - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    Image Search Results