edta mmei  (New England Biolabs)


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    Structured Review

    New England Biolabs edta mmei
    Edta Mmei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta mmei/product/New England Biolabs
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    edta mmei - by Bioz Stars, 2020-02
    79/100 stars

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    Related Articles

    Marker:

    Article Title: DNase-seq: a high-resolution technique for mapping active gene regulatory elements across the genome from mammalian cells
    Article Snippet: Baker) DNA ladder (25 bp) (Invitrogen) DNA Polymerase Buffer (1X; 10 mM Tris-Cl, pH 8.0, 50 mM NaCl, 10 mM MgCl2 , 1 mM dithiothreitol) DNase I recombinant, RNase free (10 U/μL) and incubation Buffer (10X) (Roche Diagnostics) dNTP, 10 mM (Roche Diagnostics) Dynal Streptavidin beads (Invitrogen Dynal M-280) EDTA (50 mM, pH 8.0) Ethanol (70% and 100%) Ethidium bromide(10 mg/ml) Glycogen (20 mg/mL) (Roche Diagnostics) InCert low melt agarose (1%, melted in sterile 50 mM EDTA pH 8.0 and stored at 4 °C) (Lonza) LIDS Buffer (10 mM Tris-Cl, pH 8.0,1% Lauryl sulfate lithium salt (Sigma), 100 mM EDTA) MmeI (2 U/μL, New England Biolabs) NaOAc (3 M, pH 5.3) NaOH (0.15 M) NEB Buffer 2 (10X; New England Biolabs) NEB Buffer 4 (10X; New England Biolabs) Igepal CA-630 (Sigma) Phenol (Invitrogen) Phenol: chloroform: Isoamyl Alcohol (25:24:1, Invitrogen) Phosphatase alkaline, shrimp (SAP; 1 U/μL, Roche Diagnostics) Phosphate Buffered Saline without Mg2+ /Ca2+ (1X, pH 7.2, GIBCO) Phusion DNA Polymerase (2 U/μL, Finnzymes) and Phusion HF reaction Buffer (5X) RSB Buffer (10 mM Tris-Cl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 ) S-adenosylmethionine (SAM; 500 μM, New England Biolabs) Spin-X filter (Fisher) T4 DNA Polymerase (New England Biolabs) TBE buffer (0.5X; 40 mM Tris-Cl, pH 8.3, 45 mM Boric Acid, 1 mM EDTA) TE Buffer (1X; 10 mM Tris-Cl, pH 8.0, 1 mM EDTA) Tris-HCl buffer (1X, pH8.0) T4 Ligase (5 U/μL) and ligation buffer (10X) (Roche Diagnostics) Trypan blue (Gibco) Ultrapure™ Agarose (Invitrogen) Ultrapure™ L.M.P. .. Agarose (Invitrogen) Yeast Chromosome PFG molecular weight marker (New England Biolabs)

    Incubation:

    Article Title: DNase-seq: a high-resolution technique for mapping active gene regulatory elements across the genome from mammalian cells
    Article Snippet: .. Baker) DNA ladder (25 bp) (Invitrogen) DNA Polymerase Buffer (1X; 10 mM Tris-Cl, pH 8.0, 50 mM NaCl, 10 mM MgCl2 , 1 mM dithiothreitol) DNase I recombinant, RNase free (10 U/μL) and incubation Buffer (10X) (Roche Diagnostics) dNTP, 10 mM (Roche Diagnostics) Dynal Streptavidin beads (Invitrogen Dynal M-280) EDTA (50 mM, pH 8.0) Ethanol (70% and 100%) Ethidium bromide(10 mg/ml) Glycogen (20 mg/mL) (Roche Diagnostics) InCert low melt agarose (1%, melted in sterile 50 mM EDTA pH 8.0 and stored at 4 °C) (Lonza) LIDS Buffer (10 mM Tris-Cl, pH 8.0,1% Lauryl sulfate lithium salt (Sigma), 100 mM EDTA) MmeI (2 U/μL, New England Biolabs) NaOAc (3 M, pH 5.3) NaOH (0.15 M) NEB Buffer 2 (10X; New England Biolabs) NEB Buffer 4 (10X; New England Biolabs) Igepal CA-630 (Sigma) Phenol (Invitrogen) Phenol: chloroform: Isoamyl Alcohol (25:24:1, Invitrogen) Phosphatase alkaline, shrimp (SAP; 1 U/μL, Roche Diagnostics) Phosphate Buffered Saline without Mg2+ /Ca2+ (1X, pH 7.2, GIBCO) Phusion DNA Polymerase (2 U/μL, Finnzymes) and Phusion HF reaction Buffer (5X) RSB Buffer (10 mM Tris-Cl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 ) S-adenosylmethionine (SAM; 500 μM, New England Biolabs) Spin-X filter (Fisher) T4 DNA Polymerase (New England Biolabs) TBE buffer (0.5X; 40 mM Tris-Cl, pH 8.3, 45 mM Boric Acid, 1 mM EDTA) TE Buffer (1X; 10 mM Tris-Cl, pH 8.0, 1 mM EDTA) Tris-HCl buffer (1X, pH8.0) T4 Ligase (5 U/μL) and ligation buffer (10X) (Roche Diagnostics) Trypan blue (Gibco) Ultrapure™ Agarose (Invitrogen) Ultrapure™ L.M.P. .. Agarose (Invitrogen) Yeast Chromosome PFG molecular weight marker (New England Biolabs)

    Recombinant:

    Article Title: DNase-seq: a high-resolution technique for mapping active gene regulatory elements across the genome from mammalian cells
    Article Snippet: .. Baker) DNA ladder (25 bp) (Invitrogen) DNA Polymerase Buffer (1X; 10 mM Tris-Cl, pH 8.0, 50 mM NaCl, 10 mM MgCl2 , 1 mM dithiothreitol) DNase I recombinant, RNase free (10 U/μL) and incubation Buffer (10X) (Roche Diagnostics) dNTP, 10 mM (Roche Diagnostics) Dynal Streptavidin beads (Invitrogen Dynal M-280) EDTA (50 mM, pH 8.0) Ethanol (70% and 100%) Ethidium bromide(10 mg/ml) Glycogen (20 mg/mL) (Roche Diagnostics) InCert low melt agarose (1%, melted in sterile 50 mM EDTA pH 8.0 and stored at 4 °C) (Lonza) LIDS Buffer (10 mM Tris-Cl, pH 8.0,1% Lauryl sulfate lithium salt (Sigma), 100 mM EDTA) MmeI (2 U/μL, New England Biolabs) NaOAc (3 M, pH 5.3) NaOH (0.15 M) NEB Buffer 2 (10X; New England Biolabs) NEB Buffer 4 (10X; New England Biolabs) Igepal CA-630 (Sigma) Phenol (Invitrogen) Phenol: chloroform: Isoamyl Alcohol (25:24:1, Invitrogen) Phosphatase alkaline, shrimp (SAP; 1 U/μL, Roche Diagnostics) Phosphate Buffered Saline without Mg2+ /Ca2+ (1X, pH 7.2, GIBCO) Phusion DNA Polymerase (2 U/μL, Finnzymes) and Phusion HF reaction Buffer (5X) RSB Buffer (10 mM Tris-Cl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 ) S-adenosylmethionine (SAM; 500 μM, New England Biolabs) Spin-X filter (Fisher) T4 DNA Polymerase (New England Biolabs) TBE buffer (0.5X; 40 mM Tris-Cl, pH 8.3, 45 mM Boric Acid, 1 mM EDTA) TE Buffer (1X; 10 mM Tris-Cl, pH 8.0, 1 mM EDTA) Tris-HCl buffer (1X, pH8.0) T4 Ligase (5 U/μL) and ligation buffer (10X) (Roche Diagnostics) Trypan blue (Gibco) Ultrapure™ Agarose (Invitrogen) Ultrapure™ L.M.P. .. Agarose (Invitrogen) Yeast Chromosome PFG molecular weight marker (New England Biolabs)

    Molecular Weight:

    Article Title: DNase-seq: a high-resolution technique for mapping active gene regulatory elements across the genome from mammalian cells
    Article Snippet: Baker) DNA ladder (25 bp) (Invitrogen) DNA Polymerase Buffer (1X; 10 mM Tris-Cl, pH 8.0, 50 mM NaCl, 10 mM MgCl2 , 1 mM dithiothreitol) DNase I recombinant, RNase free (10 U/μL) and incubation Buffer (10X) (Roche Diagnostics) dNTP, 10 mM (Roche Diagnostics) Dynal Streptavidin beads (Invitrogen Dynal M-280) EDTA (50 mM, pH 8.0) Ethanol (70% and 100%) Ethidium bromide(10 mg/ml) Glycogen (20 mg/mL) (Roche Diagnostics) InCert low melt agarose (1%, melted in sterile 50 mM EDTA pH 8.0 and stored at 4 °C) (Lonza) LIDS Buffer (10 mM Tris-Cl, pH 8.0,1% Lauryl sulfate lithium salt (Sigma), 100 mM EDTA) MmeI (2 U/μL, New England Biolabs) NaOAc (3 M, pH 5.3) NaOH (0.15 M) NEB Buffer 2 (10X; New England Biolabs) NEB Buffer 4 (10X; New England Biolabs) Igepal CA-630 (Sigma) Phenol (Invitrogen) Phenol: chloroform: Isoamyl Alcohol (25:24:1, Invitrogen) Phosphatase alkaline, shrimp (SAP; 1 U/μL, Roche Diagnostics) Phosphate Buffered Saline without Mg2+ /Ca2+ (1X, pH 7.2, GIBCO) Phusion DNA Polymerase (2 U/μL, Finnzymes) and Phusion HF reaction Buffer (5X) RSB Buffer (10 mM Tris-Cl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 ) S-adenosylmethionine (SAM; 500 μM, New England Biolabs) Spin-X filter (Fisher) T4 DNA Polymerase (New England Biolabs) TBE buffer (0.5X; 40 mM Tris-Cl, pH 8.3, 45 mM Boric Acid, 1 mM EDTA) TE Buffer (1X; 10 mM Tris-Cl, pH 8.0, 1 mM EDTA) Tris-HCl buffer (1X, pH8.0) T4 Ligase (5 U/μL) and ligation buffer (10X) (Roche Diagnostics) Trypan blue (Gibco) Ultrapure™ Agarose (Invitrogen) Ultrapure™ L.M.P. .. Agarose (Invitrogen) Yeast Chromosome PFG molecular weight marker (New England Biolabs)

    Ligation:

    Article Title: DNase-seq: a high-resolution technique for mapping active gene regulatory elements across the genome from mammalian cells
    Article Snippet: .. Baker) DNA ladder (25 bp) (Invitrogen) DNA Polymerase Buffer (1X; 10 mM Tris-Cl, pH 8.0, 50 mM NaCl, 10 mM MgCl2 , 1 mM dithiothreitol) DNase I recombinant, RNase free (10 U/μL) and incubation Buffer (10X) (Roche Diagnostics) dNTP, 10 mM (Roche Diagnostics) Dynal Streptavidin beads (Invitrogen Dynal M-280) EDTA (50 mM, pH 8.0) Ethanol (70% and 100%) Ethidium bromide(10 mg/ml) Glycogen (20 mg/mL) (Roche Diagnostics) InCert low melt agarose (1%, melted in sterile 50 mM EDTA pH 8.0 and stored at 4 °C) (Lonza) LIDS Buffer (10 mM Tris-Cl, pH 8.0,1% Lauryl sulfate lithium salt (Sigma), 100 mM EDTA) MmeI (2 U/μL, New England Biolabs) NaOAc (3 M, pH 5.3) NaOH (0.15 M) NEB Buffer 2 (10X; New England Biolabs) NEB Buffer 4 (10X; New England Biolabs) Igepal CA-630 (Sigma) Phenol (Invitrogen) Phenol: chloroform: Isoamyl Alcohol (25:24:1, Invitrogen) Phosphatase alkaline, shrimp (SAP; 1 U/μL, Roche Diagnostics) Phosphate Buffered Saline without Mg2+ /Ca2+ (1X, pH 7.2, GIBCO) Phusion DNA Polymerase (2 U/μL, Finnzymes) and Phusion HF reaction Buffer (5X) RSB Buffer (10 mM Tris-Cl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 ) S-adenosylmethionine (SAM; 500 μM, New England Biolabs) Spin-X filter (Fisher) T4 DNA Polymerase (New England Biolabs) TBE buffer (0.5X; 40 mM Tris-Cl, pH 8.3, 45 mM Boric Acid, 1 mM EDTA) TE Buffer (1X; 10 mM Tris-Cl, pH 8.0, 1 mM EDTA) Tris-HCl buffer (1X, pH8.0) T4 Ligase (5 U/μL) and ligation buffer (10X) (Roche Diagnostics) Trypan blue (Gibco) Ultrapure™ Agarose (Invitrogen) Ultrapure™ L.M.P. .. Agarose (Invitrogen) Yeast Chromosome PFG molecular weight marker (New England Biolabs)

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  • Team
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  • Bioz Stars
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  • 79
    New England Biolabs edta mmei
    Edta Mmei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta mmei/product/New England Biolabs
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    edta mmei - by Bioz Stars, 2020-02
    79/100 stars
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