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Sarstedt edta microtubes
Edta Microtubes, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/edta microtubes/product/Sarstedt
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
edta microtubes - by Bioz Stars, 2021-07
86/100 stars

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Transferring:

Article Title: On-demand Labeling of SNAP-tagged Viral Protein for Pulse-Chase Imaging, Quench-Pulse-Chase Imaging, and Nanoscopy-based Inspection of Cell Lysates
Article Snippet: Live cell imaging solution (enough to add 2 ml of solution to three 35-mm dishes in Steps 10, 12, and optional Step 13) 2.7 ml Fetal Calf Serum 270 μl HEPES 270 μl Glutamax-I 270 μl MEM Nonessential Amino Acids 270-540 μl ProLong Live Antifade Reagent 22.96-23.22 ml FluoroBrite DMEM The total volume of live cell imaging solution: 27 ml Tissue culture medium containing serum 500 ml Dulbecco's modified Eagle's medium 56.5 ml Fetal Calf Serum 5.6 ml MEM Nonessential amino acids solution 5.6 ml HEPES .. Micropipette tips, serological pipettes, pipette aids, and microtubes for liquid handling TipOne® 1,000 μl XL filter tips (catalog number: S1122-1830) TipOne® 200 μl filter tips (catalog number: S1120-8810) TipOne® 20 μl filter tips (catalog number: S1120-1810) or SARSTEDT pipette tip 10 μl (catalog number: 70.1130.600) Fisherbrand™ 5 ml serological pipets (catalog number: 13-676-10H) Fisherbrand™ 10 ml serological pipets (catalog number: 13-676-10J) Fisherbrand™ 25 ml serological pipets (catalog number: 13-676-10K) Drummond Pipet-Aid XL (catalog number: 4-000-205) Microtube 1.5 ml (SARSTEDT, catalog number: 72.690.001) 15 ml Centrifuge Tubes, Conical, Sterile (Starlab, catalog number: E1415-0200) Glass slide (Academy, catalog number: N/A142) Filter paper (Whatman® , catalog number: 1001025) 24-well plates (CytoOne® , catalog number: CC7682-7524) Clean, sterile 13 mm coverslips (# 1.5) (Academy, catalog number: NPS16/1818) Parafilm® M (Bemis, catalog number: PM-996) Aluminum foil (Caterwrap, catalog number: AKL-300-030M) 10-cm dishes Stable CHIKV cells (see ) Specific reagents for our cell line (for basic tissue culture), which we derived from the hepatoma cell line HuH-7: Dulbecco's modified Eagle's medium (Sigma, catalog number: D6429-500ML) 100% Fetal Calf Serum (Gibco, catalog number: 10500-064) Gibco MEM Non-essential amino acids solution (100x), store at 4 °C up to 24 months from the date of manufacture (Thermo Fisher Scientific, catalog number: 11140050) Gibco HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) buffer (100x), store at 4 °C up to 24 months from the date of manufacture (Thermo Fisher Scientific, catalog number: 15630130) Trypsin EDTA (Sigma, catalog number: T3924-500ML) PBS, Phosphate Buffered Saline (VWR Lifescience, catalog number: E404-100TABS) Antibody for immunolabeling of the SNAP-tagged protein Note: We use a rabbit antibody produced in-house that detects CHIKV nsP3 ( and ) . .. Goat anti-rabbit IgG (H+L) Cross-Absorbed Secondary Antibody, DyLight® 405 (Thermo Fisher Scientific, Invitrogen, catalog number: 35551) Note: The combination of Dylight ® 405, ZsGreen, and TMR-Star provides effective color separation and sensitivity for three-color imaging .

Derivative Assay:

Article Title: On-demand Labeling of SNAP-tagged Viral Protein for Pulse-Chase Imaging, Quench-Pulse-Chase Imaging, and Nanoscopy-based Inspection of Cell Lysates
Article Snippet: Live cell imaging solution (enough to add 2 ml of solution to three 35-mm dishes in Steps 10, 12, and optional Step 13) 2.7 ml Fetal Calf Serum 270 μl HEPES 270 μl Glutamax-I 270 μl MEM Nonessential Amino Acids 270-540 μl ProLong Live Antifade Reagent 22.96-23.22 ml FluoroBrite DMEM The total volume of live cell imaging solution: 27 ml Tissue culture medium containing serum 500 ml Dulbecco's modified Eagle's medium 56.5 ml Fetal Calf Serum 5.6 ml MEM Nonessential amino acids solution 5.6 ml HEPES .. Micropipette tips, serological pipettes, pipette aids, and microtubes for liquid handling TipOne® 1,000 μl XL filter tips (catalog number: S1122-1830) TipOne® 200 μl filter tips (catalog number: S1120-8810) TipOne® 20 μl filter tips (catalog number: S1120-1810) or SARSTEDT pipette tip 10 μl (catalog number: 70.1130.600) Fisherbrand™ 5 ml serological pipets (catalog number: 13-676-10H) Fisherbrand™ 10 ml serological pipets (catalog number: 13-676-10J) Fisherbrand™ 25 ml serological pipets (catalog number: 13-676-10K) Drummond Pipet-Aid XL (catalog number: 4-000-205) Microtube 1.5 ml (SARSTEDT, catalog number: 72.690.001) 15 ml Centrifuge Tubes, Conical, Sterile (Starlab, catalog number: E1415-0200) Glass slide (Academy, catalog number: N/A142) Filter paper (Whatman® , catalog number: 1001025) 24-well plates (CytoOne® , catalog number: CC7682-7524) Clean, sterile 13 mm coverslips (# 1.5) (Academy, catalog number: NPS16/1818) Parafilm® M (Bemis, catalog number: PM-996) Aluminum foil (Caterwrap, catalog number: AKL-300-030M) 10-cm dishes Stable CHIKV cells (see ) Specific reagents for our cell line (for basic tissue culture), which we derived from the hepatoma cell line HuH-7: Dulbecco's modified Eagle's medium (Sigma, catalog number: D6429-500ML) 100% Fetal Calf Serum (Gibco, catalog number: 10500-064) Gibco MEM Non-essential amino acids solution (100x), store at 4 °C up to 24 months from the date of manufacture (Thermo Fisher Scientific, catalog number: 11140050) Gibco HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) buffer (100x), store at 4 °C up to 24 months from the date of manufacture (Thermo Fisher Scientific, catalog number: 15630130) Trypsin EDTA (Sigma, catalog number: T3924-500ML) PBS, Phosphate Buffered Saline (VWR Lifescience, catalog number: E404-100TABS) Antibody for immunolabeling of the SNAP-tagged protein Note: We use a rabbit antibody produced in-house that detects CHIKV nsP3 ( and ) . .. Goat anti-rabbit IgG (H+L) Cross-Absorbed Secondary Antibody, DyLight® 405 (Thermo Fisher Scientific, Invitrogen, catalog number: 35551) Note: The combination of Dylight ® 405, ZsGreen, and TMR-Star provides effective color separation and sensitivity for three-color imaging .

Modification:

Article Title: On-demand Labeling of SNAP-tagged Viral Protein for Pulse-Chase Imaging, Quench-Pulse-Chase Imaging, and Nanoscopy-based Inspection of Cell Lysates
Article Snippet: Live cell imaging solution (enough to add 2 ml of solution to three 35-mm dishes in Steps 10, 12, and optional Step 13) 2.7 ml Fetal Calf Serum 270 μl HEPES 270 μl Glutamax-I 270 μl MEM Nonessential Amino Acids 270-540 μl ProLong Live Antifade Reagent 22.96-23.22 ml FluoroBrite DMEM The total volume of live cell imaging solution: 27 ml Tissue culture medium containing serum 500 ml Dulbecco's modified Eagle's medium 56.5 ml Fetal Calf Serum 5.6 ml MEM Nonessential amino acids solution 5.6 ml HEPES .. Micropipette tips, serological pipettes, pipette aids, and microtubes for liquid handling TipOne® 1,000 μl XL filter tips (catalog number: S1122-1830) TipOne® 200 μl filter tips (catalog number: S1120-8810) TipOne® 20 μl filter tips (catalog number: S1120-1810) or SARSTEDT pipette tip 10 μl (catalog number: 70.1130.600) Fisherbrand™ 5 ml serological pipets (catalog number: 13-676-10H) Fisherbrand™ 10 ml serological pipets (catalog number: 13-676-10J) Fisherbrand™ 25 ml serological pipets (catalog number: 13-676-10K) Drummond Pipet-Aid XL (catalog number: 4-000-205) Microtube 1.5 ml (SARSTEDT, catalog number: 72.690.001) 15 ml Centrifuge Tubes, Conical, Sterile (Starlab, catalog number: E1415-0200) Glass slide (Academy, catalog number: N/A142) Filter paper (Whatman® , catalog number: 1001025) 24-well plates (CytoOne® , catalog number: CC7682-7524) Clean, sterile 13 mm coverslips (# 1.5) (Academy, catalog number: NPS16/1818) Parafilm® M (Bemis, catalog number: PM-996) Aluminum foil (Caterwrap, catalog number: AKL-300-030M) 10-cm dishes Stable CHIKV cells (see ) Specific reagents for our cell line (for basic tissue culture), which we derived from the hepatoma cell line HuH-7: Dulbecco's modified Eagle's medium (Sigma, catalog number: D6429-500ML) 100% Fetal Calf Serum (Gibco, catalog number: 10500-064) Gibco MEM Non-essential amino acids solution (100x), store at 4 °C up to 24 months from the date of manufacture (Thermo Fisher Scientific, catalog number: 11140050) Gibco HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) buffer (100x), store at 4 °C up to 24 months from the date of manufacture (Thermo Fisher Scientific, catalog number: 15630130) Trypsin EDTA (Sigma, catalog number: T3924-500ML) PBS, Phosphate Buffered Saline (VWR Lifescience, catalog number: E404-100TABS) Antibody for immunolabeling of the SNAP-tagged protein Note: We use a rabbit antibody produced in-house that detects CHIKV nsP3 ( and ) . .. Goat anti-rabbit IgG (H+L) Cross-Absorbed Secondary Antibody, DyLight® 405 (Thermo Fisher Scientific, Invitrogen, catalog number: 35551) Note: The combination of Dylight ® 405, ZsGreen, and TMR-Star provides effective color separation and sensitivity for three-color imaging .

Immunolabeling:

Article Title: On-demand Labeling of SNAP-tagged Viral Protein for Pulse-Chase Imaging, Quench-Pulse-Chase Imaging, and Nanoscopy-based Inspection of Cell Lysates
Article Snippet: Live cell imaging solution (enough to add 2 ml of solution to three 35-mm dishes in Steps 10, 12, and optional Step 13) 2.7 ml Fetal Calf Serum 270 μl HEPES 270 μl Glutamax-I 270 μl MEM Nonessential Amino Acids 270-540 μl ProLong Live Antifade Reagent 22.96-23.22 ml FluoroBrite DMEM The total volume of live cell imaging solution: 27 ml Tissue culture medium containing serum 500 ml Dulbecco's modified Eagle's medium 56.5 ml Fetal Calf Serum 5.6 ml MEM Nonessential amino acids solution 5.6 ml HEPES .. Micropipette tips, serological pipettes, pipette aids, and microtubes for liquid handling TipOne® 1,000 μl XL filter tips (catalog number: S1122-1830) TipOne® 200 μl filter tips (catalog number: S1120-8810) TipOne® 20 μl filter tips (catalog number: S1120-1810) or SARSTEDT pipette tip 10 μl (catalog number: 70.1130.600) Fisherbrand™ 5 ml serological pipets (catalog number: 13-676-10H) Fisherbrand™ 10 ml serological pipets (catalog number: 13-676-10J) Fisherbrand™ 25 ml serological pipets (catalog number: 13-676-10K) Drummond Pipet-Aid XL (catalog number: 4-000-205) Microtube 1.5 ml (SARSTEDT, catalog number: 72.690.001) 15 ml Centrifuge Tubes, Conical, Sterile (Starlab, catalog number: E1415-0200) Glass slide (Academy, catalog number: N/A142) Filter paper (Whatman® , catalog number: 1001025) 24-well plates (CytoOne® , catalog number: CC7682-7524) Clean, sterile 13 mm coverslips (# 1.5) (Academy, catalog number: NPS16/1818) Parafilm® M (Bemis, catalog number: PM-996) Aluminum foil (Caterwrap, catalog number: AKL-300-030M) 10-cm dishes Stable CHIKV cells (see ) Specific reagents for our cell line (for basic tissue culture), which we derived from the hepatoma cell line HuH-7: Dulbecco's modified Eagle's medium (Sigma, catalog number: D6429-500ML) 100% Fetal Calf Serum (Gibco, catalog number: 10500-064) Gibco MEM Non-essential amino acids solution (100x), store at 4 °C up to 24 months from the date of manufacture (Thermo Fisher Scientific, catalog number: 11140050) Gibco HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) buffer (100x), store at 4 °C up to 24 months from the date of manufacture (Thermo Fisher Scientific, catalog number: 15630130) Trypsin EDTA (Sigma, catalog number: T3924-500ML) PBS, Phosphate Buffered Saline (VWR Lifescience, catalog number: E404-100TABS) Antibody for immunolabeling of the SNAP-tagged protein Note: We use a rabbit antibody produced in-house that detects CHIKV nsP3 ( and ) . .. Goat anti-rabbit IgG (H+L) Cross-Absorbed Secondary Antibody, DyLight® 405 (Thermo Fisher Scientific, Invitrogen, catalog number: 35551) Note: The combination of Dylight ® 405, ZsGreen, and TMR-Star provides effective color separation and sensitivity for three-color imaging .

Produced:

Article Title: On-demand Labeling of SNAP-tagged Viral Protein for Pulse-Chase Imaging, Quench-Pulse-Chase Imaging, and Nanoscopy-based Inspection of Cell Lysates
Article Snippet: Live cell imaging solution (enough to add 2 ml of solution to three 35-mm dishes in Steps 10, 12, and optional Step 13) 2.7 ml Fetal Calf Serum 270 μl HEPES 270 μl Glutamax-I 270 μl MEM Nonessential Amino Acids 270-540 μl ProLong Live Antifade Reagent 22.96-23.22 ml FluoroBrite DMEM The total volume of live cell imaging solution: 27 ml Tissue culture medium containing serum 500 ml Dulbecco's modified Eagle's medium 56.5 ml Fetal Calf Serum 5.6 ml MEM Nonessential amino acids solution 5.6 ml HEPES .. Micropipette tips, serological pipettes, pipette aids, and microtubes for liquid handling TipOne® 1,000 μl XL filter tips (catalog number: S1122-1830) TipOne® 200 μl filter tips (catalog number: S1120-8810) TipOne® 20 μl filter tips (catalog number: S1120-1810) or SARSTEDT pipette tip 10 μl (catalog number: 70.1130.600) Fisherbrand™ 5 ml serological pipets (catalog number: 13-676-10H) Fisherbrand™ 10 ml serological pipets (catalog number: 13-676-10J) Fisherbrand™ 25 ml serological pipets (catalog number: 13-676-10K) Drummond Pipet-Aid XL (catalog number: 4-000-205) Microtube 1.5 ml (SARSTEDT, catalog number: 72.690.001) 15 ml Centrifuge Tubes, Conical, Sterile (Starlab, catalog number: E1415-0200) Glass slide (Academy, catalog number: N/A142) Filter paper (Whatman® , catalog number: 1001025) 24-well plates (CytoOne® , catalog number: CC7682-7524) Clean, sterile 13 mm coverslips (# 1.5) (Academy, catalog number: NPS16/1818) Parafilm® M (Bemis, catalog number: PM-996) Aluminum foil (Caterwrap, catalog number: AKL-300-030M) 10-cm dishes Stable CHIKV cells (see ) Specific reagents for our cell line (for basic tissue culture), which we derived from the hepatoma cell line HuH-7: Dulbecco's modified Eagle's medium (Sigma, catalog number: D6429-500ML) 100% Fetal Calf Serum (Gibco, catalog number: 10500-064) Gibco MEM Non-essential amino acids solution (100x), store at 4 °C up to 24 months from the date of manufacture (Thermo Fisher Scientific, catalog number: 11140050) Gibco HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) buffer (100x), store at 4 °C up to 24 months from the date of manufacture (Thermo Fisher Scientific, catalog number: 15630130) Trypsin EDTA (Sigma, catalog number: T3924-500ML) PBS, Phosphate Buffered Saline (VWR Lifescience, catalog number: E404-100TABS) Antibody for immunolabeling of the SNAP-tagged protein Note: We use a rabbit antibody produced in-house that detects CHIKV nsP3 ( and ) . .. Goat anti-rabbit IgG (H+L) Cross-Absorbed Secondary Antibody, DyLight® 405 (Thermo Fisher Scientific, Invitrogen, catalog number: 35551) Note: The combination of Dylight ® 405, ZsGreen, and TMR-Star provides effective color separation and sensitivity for three-color imaging .

Isolation:

Article Title: Plasma Exosomes Contribute to Microvascular Damage in Diabetic Retinopathy by Activating the Classical Complement Pathway
Article Snippet: We used ImageJ software to quantify the blots. .. Exosome Isolation Blood was collected from the inferior vena cava into EDTA tubes (Microvette 300; SARSTEDT AG & Co.), separated via centrifugation, and either used immediately or aliquoted into 1.5-mL microtubes and stored at −80°C. .. The ExoQuick system (System Biosciences) was used to purify plasma exosomes according to the manufacturer’s protocol.

Centrifugation:

Article Title: Plasma Exosomes Contribute to Microvascular Damage in Diabetic Retinopathy by Activating the Classical Complement Pathway
Article Snippet: We used ImageJ software to quantify the blots. .. Exosome Isolation Blood was collected from the inferior vena cava into EDTA tubes (Microvette 300; SARSTEDT AG & Co.), separated via centrifugation, and either used immediately or aliquoted into 1.5-mL microtubes and stored at −80°C. .. The ExoQuick system (System Biosciences) was used to purify plasma exosomes according to the manufacturer’s protocol.

Article Title: Bacteroides uniformis combined with fiber amplifies metabolic and immune benefits in obese mice
Article Snippet: .. Blood metabolic parametersBlood from the mandibular vein of 4-hour fasted mice was collected at week 12 in microtubes with K3 EDTA (Sarstedt, Nümbrecht Germany) to determine glycemia, measured as conducted in the OGTT, and triglycerides and cholesterol in plasma (blood centrifugation at 400 g for 15 minutes at 4°C) using a colorimetric kit assay according to manufacturer´s instructions (Química Clínica Aplicada, Tarragona, Spain). .. Plasma from blood collected by cardiac puncture in 4-hour fasted mice at week 17 was used to measure insulin, glucagon-like peptide 1 (GLP-1), peptide YY (PYY) and the cytokine tumor necrosis factor α (TNFα), interleukins IL12, IL10, IL4, and IL22 and interferon ɤ (IFNɤ) using Multiplex Assays Using Luminex® (Milliplex, Merck group, Darmstadt, Germany) according to manufacturer´s instructions.

Mouse Assay:

Article Title: Bacteroides uniformis combined with fiber amplifies metabolic and immune benefits in obese mice
Article Snippet: .. Blood metabolic parametersBlood from the mandibular vein of 4-hour fasted mice was collected at week 12 in microtubes with K3 EDTA (Sarstedt, Nümbrecht Germany) to determine glycemia, measured as conducted in the OGTT, and triglycerides and cholesterol in plasma (blood centrifugation at 400 g for 15 minutes at 4°C) using a colorimetric kit assay according to manufacturer´s instructions (Química Clínica Aplicada, Tarragona, Spain). .. Plasma from blood collected by cardiac puncture in 4-hour fasted mice at week 17 was used to measure insulin, glucagon-like peptide 1 (GLP-1), peptide YY (PYY) and the cytokine tumor necrosis factor α (TNFα), interleukins IL12, IL10, IL4, and IL22 and interferon ɤ (IFNɤ) using Multiplex Assays Using Luminex® (Milliplex, Merck group, Darmstadt, Germany) according to manufacturer´s instructions.

other:

Article Title: SHIP1 Regulates MSC Numbers and Their Osteolineage Commitment by Limiting Induction of the PI3K/Akt/β-Catenin/Id2 Axis
Article Snippet: Peripheral blood samples were harvested in EDTA-coated microtubes (Sarstedt Co.) by submandibular bleeding and analyzed with a HEMAVET 950S Veterinary Hematology Analyzer (Drew Scientific, Inc.) using the mouse species program.

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    Sarstedt edta coated microtubes
    Edta Coated Microtubes, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta coated microtubes/product/Sarstedt
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    edta coated microtubes - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    86
    Sarstedt edta containing microtubes
    Edta Containing Microtubes, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta containing microtubes/product/Sarstedt
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    edta containing microtubes - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

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