edta ferric sodium salt  (Millipore)


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  • 99
    Name:
    Ethylenediaminetetraacetic acid ferric sodium salt
    Description:

    Catalog Number:
    e6760
    Price:
    None
    Applications:
    Used to eliminate enzyme inhibition by traces of heavy metals, and to inhibit enzymes that require divalent cations as cofactors. Iron-EDTA enhances damage due to reactive oxygen species, such as depletion of reduced glutathione, formation of methemoglobin, and lipid peroxidation.
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    Structured Review

    Millipore edta ferric sodium salt
    Ethylenediaminetetraacetic acid ferric sodium salt

    https://www.bioz.com/result/edta ferric sodium salt/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    edta ferric sodium salt - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Improving Combination Osteoporosis Therapy in a Preclinical Model of Heightened Osteoanabolism
    Article Snippet: Paragraph title: Immunohistochemistry ... Subsequently, slides were transferred to 0.2 M acetate buffer (pH 5.0) for 20 minutes at room temperature and then placed in medium containing napthol AS-MX phosphate (0.5 mg/mL; Sigma-Aldrich, St. Louis, MO; N4875) and fast red TR salt (1.1 mg/mL; Sigma-Aldrich; E6760) in acetate buffer for 60 minutes at 37°C before counterstaining with toluidine blue.

    Mutagenesis:

    Article Title: Aequorin variants with improved bioluminescence properties
    Article Snippet: Tris(hydroxymethyl)amino methane (Tris), ethylenediaminetetraacetic acid (EDTA) sodium salt, agar, glucose, magnesium sulfate, magnesium chloride and all other reagents were from Sigma (St Louis, MO, USA). .. QuikChange™ site-directed mutagenesis kit was obtained from Stratagene (La Jolla, CA, USA).

    Centrifugation:

    Article Title: Cloning, Sequencing, and Phenotypic Characterization of the rpoS Gene from Pseudomonas putida KT2440
    Article Snippet: The minimal medium used for growth was either AB ( ) supplemented with 0.01 mM Fe · EDTA (catalog no. E6760; Sigma, St. Louis, Mo.) and 10 mM sodium citrate or modified M9 minimal medium ( ) supplemented with 10 mM sodium citrate. .. Carbon starvation was imposed as described by Givskov et al. , either by harvesting a growing culture (optical density at 450 nm [OD450 ] of 0.4) by centrifugation (preheated rotor and tubes at 30°C) followed by resuspension in preheated carbon-free minimal medium (AB or M9) or by exhaustion of the carbon source in AB or M9 medium supplemented with 1 mM sodium citrate (this condition resulted in starved cultures with an OD450 of 0.4).

    Cell Culture:

    Article Title: Discovery and functional evaluation of ciliary proteins in Tetrahymena thermophila
    Article Snippet: .. Tetrahymena strains (available from the Tetrahymena Stock Center) are routinely cultured in SPPA ( ) medium containing 1% proteose-peptone (Difco 211684), 0.1% yeast extract, 0.2% glucose, 0.003% EDTA ferric sodium salt (Sigma-Aldrich E6760) and 1% antibiotic-antimycotic mix (VWR 12001-712). ..

    Concentration Assay:

    Article Title: Induction of Capsule Growth in Cryptococcus neoformans by Mammalian Serum and CO2
    Article Snippet: .. To supplement the media with iron, EDTA-ferric sodium salt (Sigma, St. Louis, Mo.) was added at the concentration indicated in each case. ..

    Incubation:

    Article Title: Improving Combination Osteoporosis Therapy in a Preclinical Model of Heightened Osteoanabolism
    Article Snippet: After washing with PBS, sections were incubated with VECTASTAIN® ABC Reagent for 30 minutes at room temperature, followed by washing in buffer for 5 minutes. .. Subsequently, slides were transferred to 0.2 M acetate buffer (pH 5.0) for 20 minutes at room temperature and then placed in medium containing napthol AS-MX phosphate (0.5 mg/mL; Sigma-Aldrich, St. Louis, MO; N4875) and fast red TR salt (1.1 mg/mL; Sigma-Aldrich; E6760) in acetate buffer for 60 minutes at 37°C before counterstaining with toluidine blue.

    Article Title: Induction of Capsule Growth in Cryptococcus neoformans by Mammalian Serum and CO2
    Article Snippet: The yeast cells (around 2 × 106 to 4 × 106 ) were placed in six-well plates containing 2 ml of medium and incubated at 37°C in the absence or presence of 10% CO2 . .. To supplement the media with iron, EDTA-ferric sodium salt (Sigma, St. Louis, Mo.) was added at the concentration indicated in each case.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Improving Combination Osteoporosis Therapy in a Preclinical Model of Heightened Osteoanabolism
    Article Snippet: In brief, formalin-fixed, paraffin-embedded sections were deparaffinized, followed by rehydration via a sequential ethanol wash. .. Subsequently, slides were transferred to 0.2 M acetate buffer (pH 5.0) for 20 minutes at room temperature and then placed in medium containing napthol AS-MX phosphate (0.5 mg/mL; Sigma-Aldrich, St. Louis, MO; N4875) and fast red TR salt (1.1 mg/mL; Sigma-Aldrich; E6760) in acetate buffer for 60 minutes at 37°C before counterstaining with toluidine blue.

    Bradford Protein Assay:

    Article Title: Aequorin variants with improved bioluminescence properties
    Article Snippet: Bovine serum albumin (BSA) and Bradford Protein Assay were purchased from BioRad (Hercules, CA, USA). .. Tris(hydroxymethyl)amino methane (Tris), ethylenediaminetetraacetic acid (EDTA) sodium salt, agar, glucose, magnesium sulfate, magnesium chloride and all other reagents were from Sigma (St Louis, MO, USA).

    Modification:

    Article Title: Improving Combination Osteoporosis Therapy in a Preclinical Model of Heightened Osteoanabolism
    Article Snippet: Tartrate-resistant acid phosphatase (TRAP) staining was performed using a modified protocol based on the method of Erlebacher and Derynck ( ). .. Subsequently, slides were transferred to 0.2 M acetate buffer (pH 5.0) for 20 minutes at room temperature and then placed in medium containing napthol AS-MX phosphate (0.5 mg/mL; Sigma-Aldrich, St. Louis, MO; N4875) and fast red TR salt (1.1 mg/mL; Sigma-Aldrich; E6760) in acetate buffer for 60 minutes at 37°C before counterstaining with toluidine blue.

    Article Title: Cloning, Sequencing, and Phenotypic Characterization of the rpoS Gene from Pseudomonas putida KT2440
    Article Snippet: .. The minimal medium used for growth was either AB ( ) supplemented with 0.01 mM Fe · EDTA (catalog no. E6760; Sigma, St. Louis, Mo.) and 10 mM sodium citrate or modified M9 minimal medium ( ) supplemented with 10 mM sodium citrate. .. Alternatively, 1.5% (wt/vol) lactose-supplemented MacConkey agar (Difco) or rich Luria-Bertani (LB) medium ( ) was used.

    Article Title: Induction of Capsule Growth in Cryptococcus neoformans by Mammalian Serum and CO2
    Article Snippet: To study capsule growth, the yeast cells were incubated at 37°C for the time interval indicated in one of the following media: PBS, Sabouraud dextrose broth, or Dulbecco's modified Eagle medium (DME; Life Technologies, Rockville, Md.). .. To supplement the media with iron, EDTA-ferric sodium salt (Sigma, St. Louis, Mo.) was added at the concentration indicated in each case.

    Staining:

    Article Title: Improving Combination Osteoporosis Therapy in a Preclinical Model of Heightened Osteoanabolism
    Article Snippet: Tartrate-resistant acid phosphatase (TRAP) staining was performed using a modified protocol based on the method of Erlebacher and Derynck ( ). .. Subsequently, slides were transferred to 0.2 M acetate buffer (pH 5.0) for 20 minutes at room temperature and then placed in medium containing napthol AS-MX phosphate (0.5 mg/mL; Sigma-Aldrich, St. Louis, MO; N4875) and fast red TR salt (1.1 mg/mL; Sigma-Aldrich; E6760) in acetate buffer for 60 minutes at 37°C before counterstaining with toluidine blue.

    other:

    Article Title: Iron Regulation of the Major Virulence Factors in the AIDS-Associated Pathogen Cryptococcus neoformansIron Regulation and an Opportunistic AIDS-Related Fungal Infection
    Article Snippet: Iron-replete medium (LIM + Fe) contained 100 μM of ethylenediaminetetraacetic acid ferric-sodium salt (FeEDTA; Sigma, St. Louis, Missouri, United States).

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  • 99
    Millipore triton x 100 buffer
    Pretreating hippocampal slices with Trolox rescues Aβ 1–42 -induced GLT-1 Triton X-100 detergent-insolubility and ubiquitination.  A , Representative Western blots show GLT-1 levels in protein lysates first subjected to three serial 1% Triton X-100 extractions to remove Triton X-100-soluble proteins. Aβ 1–42  increased detergent-insoluble GLT-1 levels compared with control slices. Trolox reduced GLT-1 detergent-insolubility to control levels. Prestaining with Sypro confirmed equivalent protein loading among lanes.  B , Aβ 1–42  increased Triton X-100-insoluble GLT-1 (left) and was rescued by Trolox pretreatment (Ctrl, Aβ 1–42 , and Aβ 1–42  pretreated 1 h before and during 30 min Aβ 1–42  treatment;  n  = 9, 50, and 18, respectively;  F (2,74)  = 13.605,  p
    Triton X 100 Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triton x 100 buffer/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    triton x 100 buffer - by Bioz Stars, 2020-03
    99/100 stars
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    Image Search Results


    Pretreating hippocampal slices with Trolox rescues Aβ 1–42 -induced GLT-1 Triton X-100 detergent-insolubility and ubiquitination.  A , Representative Western blots show GLT-1 levels in protein lysates first subjected to three serial 1% Triton X-100 extractions to remove Triton X-100-soluble proteins. Aβ 1–42  increased detergent-insoluble GLT-1 levels compared with control slices. Trolox reduced GLT-1 detergent-insolubility to control levels. Prestaining with Sypro confirmed equivalent protein loading among lanes.  B , Aβ 1–42  increased Triton X-100-insoluble GLT-1 (left) and was rescued by Trolox pretreatment (Ctrl, Aβ 1–42 , and Aβ 1–42  pretreated 1 h before and during 30 min Aβ 1–42  treatment;  n  = 9, 50, and 18, respectively;  F (2,74)  = 13.605,  p

    Journal: The Journal of Neuroscience

    Article Title: Amyloid-?1–42 Slows Clearance of Synaptically Released Glutamate by Mislocalizing Astrocytic GLT-1

    doi: 10.1523/JNEUROSCI.5274-12.2013

    Figure Lengend Snippet: Pretreating hippocampal slices with Trolox rescues Aβ 1–42 -induced GLT-1 Triton X-100 detergent-insolubility and ubiquitination. A , Representative Western blots show GLT-1 levels in protein lysates first subjected to three serial 1% Triton X-100 extractions to remove Triton X-100-soluble proteins. Aβ 1–42 increased detergent-insoluble GLT-1 levels compared with control slices. Trolox reduced GLT-1 detergent-insolubility to control levels. Prestaining with Sypro confirmed equivalent protein loading among lanes. B , Aβ 1–42 increased Triton X-100-insoluble GLT-1 (left) and was rescued by Trolox pretreatment (Ctrl, Aβ 1–42 , and Aβ 1–42 pretreated 1 h before and during 30 min Aβ 1–42 treatment; n = 9, 50, and 18, respectively; F (2,74) = 13.605, p

    Article Snippet: Protein lysates from slices and cultured astrocytes were prepared using 1% Triton X-100 buffer [in m m : 20 Tris, 1 EDTA, 0.5 EGTA, 250 sucrose, Protease Inhibitor Cocktail (EMD Millipore)].

    Techniques: Western Blot