Structured Review

Cell Signaling Technology Inc ecorv
Ecorv, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ecorv/product/Cell Signaling Technology Inc
Average 94 stars, based on 4 article reviews
Price from $9.99 to $1999.99
ecorv - by Bioz Stars, 2020-07
94/100 stars

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Sequencing:

Article Title: Intragenic regulation of SOCS3 isoforms
Article Snippet: .. The DNA sequence coding for SOCS3 cds M12V was amplified from pcDNA3-[SOCS3 cds M12V] using the primers fw: 5′-TATCTGAAGCTTATGGTCACCCACAGCA-3′; rv: 5′-GTCGGCGATATCTGC CCTTTGCGCCC TTTA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 cds M12V]. .. The DNA sequence coding for SOCS3 pre-mRNA was amplified from pcDNA3-[SOCS3 pre-mRNA] using the primers fw: 5′-TATCTGAAGCTTGCGGCTCCGACTTGGA-3′; rv: 5′-ATGAGATATCTCTAGAGTTTTTCATTAA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 pre-mRNA].

Article Title: Intragenic regulation of SOCS3 isoforms
Article Snippet: .. The DNA sequence coding for SOCS3 pre-mRNA was amplified from pcDNA3-[SOCS3 pre-mRNA] using the primers fw: 5′-TATCTGAAGCTTGCGGCTCCGACTTGGA-3′; rv: 5′-ATGAGATATCTCTAGAGTTTTTCATTAA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 pre-mRNA]. .. We were very cautious not to affect the Kozak sequences within the described constructs.

Article Title: Intragenic regulation of SOCS3 isoforms
Article Snippet: .. The DNA sequence coding for SOCS3 cds dN was amplified from pcDNA3-[SOCS3 cds dN] using the primers fw: 5′-TATCTGAAGCTTATGAGCCGCCCCCTGGACA-3′; rv: 5′-GTCGGCGATATCTGCCCTTTGCG CCCTTTA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 cds dN]. .. The DNA sequence coding for SOCS3 cds M12V was amplified from pcDNA3-[SOCS3 cds M12V] using the primers fw: 5′-TATCTGAAGCTTATGGTCACCCACAGCA-3′; rv: 5′-GTCGGCGATATCTGC CCTTTGCGCCC TTTA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 cds M12V].

Article Title: Intragenic regulation of SOCS3 isoforms
Article Snippet: .. SOCS3 coding sequence was amplified from pcDNA3-[SOCS3 cds] using the primers fw: 5′-TATCTGAAGCTTATGGTCACCCACAGCA-3′; rv: 5′-GTCGGCGATATCTGCCCTTTGCGCCCTTTA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 cds]. .. The DNA sequence coding for SOCS3 cds dN was amplified from pcDNA3-[SOCS3 cds dN] using the primers fw: 5′-TATCTGAAGCTTATGAGCCGCCCCCTGGACA-3′; rv: 5′-GTCGGCGATATCTGCCCTTTGCG CCCTTTA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 cds dN].

Clone Assay:

Article Title: Intragenic regulation of SOCS3 isoforms
Article Snippet: .. The DNA sequence coding for SOCS3 cds M12V was amplified from pcDNA3-[SOCS3 cds M12V] using the primers fw: 5′-TATCTGAAGCTTATGGTCACCCACAGCA-3′; rv: 5′-GTCGGCGATATCTGC CCTTTGCGCCC TTTA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 cds M12V]. .. The DNA sequence coding for SOCS3 pre-mRNA was amplified from pcDNA3-[SOCS3 pre-mRNA] using the primers fw: 5′-TATCTGAAGCTTGCGGCTCCGACTTGGA-3′; rv: 5′-ATGAGATATCTCTAGAGTTTTTCATTAA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 pre-mRNA].

Article Title: Intragenic regulation of SOCS3 isoforms
Article Snippet: .. The DNA sequence coding for SOCS3 pre-mRNA was amplified from pcDNA3-[SOCS3 pre-mRNA] using the primers fw: 5′-TATCTGAAGCTTGCGGCTCCGACTTGGA-3′; rv: 5′-ATGAGATATCTCTAGAGTTTTTCATTAA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 pre-mRNA]. .. We were very cautious not to affect the Kozak sequences within the described constructs.

Article Title: Intragenic regulation of SOCS3 isoforms
Article Snippet: .. The DNA sequence coding for SOCS3 cds dN was amplified from pcDNA3-[SOCS3 cds dN] using the primers fw: 5′-TATCTGAAGCTTATGAGCCGCCCCCTGGACA-3′; rv: 5′-GTCGGCGATATCTGCCCTTTGCG CCCTTTA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 cds dN]. .. The DNA sequence coding for SOCS3 cds M12V was amplified from pcDNA3-[SOCS3 cds M12V] using the primers fw: 5′-TATCTGAAGCTTATGGTCACCCACAGCA-3′; rv: 5′-GTCGGCGATATCTGC CCTTTGCGCCC TTTA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 cds M12V].

Article Title: Intragenic regulation of SOCS3 isoforms
Article Snippet: .. SOCS3 coding sequence was amplified from pcDNA3-[SOCS3 cds] using the primers fw: 5′-TATCTGAAGCTTATGGTCACCCACAGCA-3′; rv: 5′-GTCGGCGATATCTGCCCTTTGCGCCCTTTA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 cds]. .. The DNA sequence coding for SOCS3 cds dN was amplified from pcDNA3-[SOCS3 cds dN] using the primers fw: 5′-TATCTGAAGCTTATGAGCCGCCCCCTGGACA-3′; rv: 5′-GTCGGCGATATCTGCCCTTTGCG CCCTTTA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 cds dN].

Amplification:

Article Title: Intragenic regulation of SOCS3 isoforms
Article Snippet: .. The DNA sequence coding for SOCS3 cds M12V was amplified from pcDNA3-[SOCS3 cds M12V] using the primers fw: 5′-TATCTGAAGCTTATGGTCACCCACAGCA-3′; rv: 5′-GTCGGCGATATCTGC CCTTTGCGCCC TTTA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 cds M12V]. .. The DNA sequence coding for SOCS3 pre-mRNA was amplified from pcDNA3-[SOCS3 pre-mRNA] using the primers fw: 5′-TATCTGAAGCTTGCGGCTCCGACTTGGA-3′; rv: 5′-ATGAGATATCTCTAGAGTTTTTCATTAA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 pre-mRNA].

Article Title: Intragenic regulation of SOCS3 isoforms
Article Snippet: .. The DNA sequence coding for SOCS3 pre-mRNA was amplified from pcDNA3-[SOCS3 pre-mRNA] using the primers fw: 5′-TATCTGAAGCTTGCGGCTCCGACTTGGA-3′; rv: 5′-ATGAGATATCTCTAGAGTTTTTCATTAA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 pre-mRNA]. .. We were very cautious not to affect the Kozak sequences within the described constructs.

Article Title: Intragenic regulation of SOCS3 isoforms
Article Snippet: .. The DNA sequence coding for SOCS3 cds dN was amplified from pcDNA3-[SOCS3 cds dN] using the primers fw: 5′-TATCTGAAGCTTATGAGCCGCCCCCTGGACA-3′; rv: 5′-GTCGGCGATATCTGCCCTTTGCG CCCTTTA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 cds dN]. .. The DNA sequence coding for SOCS3 cds M12V was amplified from pcDNA3-[SOCS3 cds M12V] using the primers fw: 5′-TATCTGAAGCTTATGGTCACCCACAGCA-3′; rv: 5′-GTCGGCGATATCTGC CCTTTGCGCCC TTTA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 cds M12V].

Article Title: Intragenic regulation of SOCS3 isoforms
Article Snippet: .. SOCS3 coding sequence was amplified from pcDNA3-[SOCS3 cds] using the primers fw: 5′-TATCTGAAGCTTATGGTCACCCACAGCA-3′; rv: 5′-GTCGGCGATATCTGCCCTTTGCGCCCTTTA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 cds]. .. The DNA sequence coding for SOCS3 cds dN was amplified from pcDNA3-[SOCS3 cds dN] using the primers fw: 5′-TATCTGAAGCTTATGAGCCGCCCCCTGGACA-3′; rv: 5′-GTCGGCGATATCTGCCCTTTGCG CCCTTTA-3′ (Thermo Fisher Scientific) and cloned into pcDNA5/FRT using HindIII and EcoRV (CST) to prepare pcDNA5/FRT-[SOCS3 cds dN].

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    Cell Signaling Technology Inc ecorv
    Ecorv, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecorv/product/Cell Signaling Technology Inc
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ecorv - by Bioz Stars, 2020-07
    94/100 stars
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