ecorv xhoi  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ecorv xhoi
    Ecorv Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecorv xhoi/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecorv xhoi - by Bioz Stars, 2020-04
    86/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Complex splicing control of the human Thrombopoietin gene by intronic G runs
    Article Snippet: .. The PCR product was EcoRV-XhoI cloned in pcDNA expression vector (Invitrogen) so generating the construct IVS2-wt (Figure 1c). .. Subsequently, the constructs carrying disruption of single or multiple G runs were obtained by PCR site directed mutagenesis.

    Amplification:

    Article Title: Complex splicing control of the human Thrombopoietin gene by intronic G runs
    Article Snippet: Fragment A was amplified using primer sense Ex1-EcoRV (5′-attggatatctgcagaattcgccctttta-3′) and primer antisense (5′-cagggtgaacagatgcattctgggaaggctacactcttctcgaca-3′), whereas fragment B was amplified using primer IVS1-s (5′-tgtcgagaagagtgtagccttcccagaatgcatctgttcaccctg-3′) and Ex4–XhoI (5′-acgtctcgagcatctgggttttccattctc-3′). .. The PCR product was EcoRV-XhoI cloned in pcDNA expression vector (Invitrogen) so generating the construct IVS2-wt (Figure 1c).

    Mutagenesis:

    Article Title: Complex splicing control of the human Thrombopoietin gene by intronic G runs
    Article Snippet: The PCR product was EcoRV-XhoI cloned in pcDNA expression vector (Invitrogen) so generating the construct IVS2-wt (Figure 1c). .. Subsequently, the constructs carrying disruption of single or multiple G runs were obtained by PCR site directed mutagenesis.

    Construct:

    Article Title: Complex splicing control of the human Thrombopoietin gene by intronic G runs
    Article Snippet: .. The PCR product was EcoRV-XhoI cloned in pcDNA expression vector (Invitrogen) so generating the construct IVS2-wt (Figure 1c). .. Subsequently, the constructs carrying disruption of single or multiple G runs were obtained by PCR site directed mutagenesis.

    Sequencing:

    Article Title: A Role for Ectophosphatase in Xenobiotic Resistance
    Article Snippet: .. The complete 4-kb cDNA fragment was cut out with StuI (which cleaves in the leader sequence) and SalI (which cuts in the polylinker of pUC12 downstream of the cDNA insert) and inserted into a EcoRV-XhoI–cleaved pcDNA I/Amp vector (Invitrogen, Carlsbad, CA); from this, it was cut out again as a BamHI fragment and finally inserted in the correct orientation into the BamHI site of the yeast expression vector pvt101U ( ). pvt101 containing MDR1 and pvt101 alone were each independently transformed into Saccharomyces cerevisiae INVSC1 (MATα his3- Δ 1 leu2 trp1-289 ura3-52 ), YMR4 (MATα his3-11,15 leu2-3 112ura3 Δ 5 can Res pho5,3 :: ura3 Δ 1 ), YPH499 ( ura3-5 2 leu2- Δ 1 his3 - Δ200 ade2-101och lys2-801a trp1-Δ63 ), and YKKA-7 ( ura3-52 leu2- Δ 1 his3-Δ200 ade2-101och lys2-801a Δpdr5 :: TRP1 ) by a polyethylene glycol–lithium acetate procedure ( ) and selected on uracil dropout medium. .. Yeast were grown at 30°C under conditions of constant selection for uracil auxotrophy.

    Article Title: Complex splicing control of the human Thrombopoietin gene by intronic G runs
    Article Snippet: The PCR product was EcoRV-XhoI cloned in pcDNA expression vector (Invitrogen) so generating the construct IVS2-wt (Figure 1c). .. All constructs were verified by sequence analysis using the CEQ 2000 sequencer machine (Beckman Coulter) to exclude the presence of mutations.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: A Role for Ectophosphatase in Xenobiotic Resistance
    Article Snippet: A 3′ part of the AtPGP1 cDNA that overlapped with the 1.9-kb 5′ part over 200 bp was obtained by RT-PCR with the oligonucleotides 5′-GGCTGCTCGAGTCGCAAATG-3′ (sense strand; sequence corresponds to nucleotide positions 2912 to 2931) and 5′-cgggatCCAAAGTAGTAAGTACTAAGC (antisense strand; sequence corresponds to positions 5822 to 5842 [ ]); the nucleotides shown in lowercase were added to provide a BamHI restriction site. .. The complete 4-kb cDNA fragment was cut out with StuI (which cleaves in the leader sequence) and SalI (which cuts in the polylinker of pUC12 downstream of the cDNA insert) and inserted into a EcoRV-XhoI–cleaved pcDNA I/Amp vector (Invitrogen, Carlsbad, CA); from this, it was cut out again as a BamHI fragment and finally inserted in the correct orientation into the BamHI site of the yeast expression vector pvt101U ( ). pvt101 containing MDR1 and pvt101 alone were each independently transformed into Saccharomyces cerevisiae INVSC1 (MATα his3- Δ 1 leu2 trp1-289 ura3-52 ), YMR4 (MATα his3-11,15 leu2-3 112ura3 Δ 5 can Res pho5,3 :: ura3 Δ 1 ), YPH499 ( ura3-5 2 leu2- Δ 1 his3 - Δ200 ade2-101och lys2-801a trp1-Δ63 ), and YKKA-7 ( ura3-52 leu2- Δ 1 his3-Δ200 ade2-101och lys2-801a Δpdr5 :: TRP1 ) by a polyethylene glycol–lithium acetate procedure ( ) and selected on uracil dropout medium.

    Generated:

    Article Title: Complex splicing control of the human Thrombopoietin gene by intronic G runs
    Article Snippet: In the first round, two overlapping PCR fragments were generated. .. The PCR product was EcoRV-XhoI cloned in pcDNA expression vector (Invitrogen) so generating the construct IVS2-wt (Figure 1c).

    Expressing:

    Article Title: A Role for Ectophosphatase in Xenobiotic Resistance
    Article Snippet: .. The complete 4-kb cDNA fragment was cut out with StuI (which cleaves in the leader sequence) and SalI (which cuts in the polylinker of pUC12 downstream of the cDNA insert) and inserted into a EcoRV-XhoI–cleaved pcDNA I/Amp vector (Invitrogen, Carlsbad, CA); from this, it was cut out again as a BamHI fragment and finally inserted in the correct orientation into the BamHI site of the yeast expression vector pvt101U ( ). pvt101 containing MDR1 and pvt101 alone were each independently transformed into Saccharomyces cerevisiae INVSC1 (MATα his3- Δ 1 leu2 trp1-289 ura3-52 ), YMR4 (MATα his3-11,15 leu2-3 112ura3 Δ 5 can Res pho5,3 :: ura3 Δ 1 ), YPH499 ( ura3-5 2 leu2- Δ 1 his3 - Δ200 ade2-101och lys2-801a trp1-Δ63 ), and YKKA-7 ( ura3-52 leu2- Δ 1 his3-Δ200 ade2-101och lys2-801a Δpdr5 :: TRP1 ) by a polyethylene glycol–lithium acetate procedure ( ) and selected on uracil dropout medium. .. Yeast were grown at 30°C under conditions of constant selection for uracil auxotrophy.

    Article Title: Complex splicing control of the human Thrombopoietin gene by intronic G runs
    Article Snippet: .. The PCR product was EcoRV-XhoI cloned in pcDNA expression vector (Invitrogen) so generating the construct IVS2-wt (Figure 1c). .. Subsequently, the constructs carrying disruption of single or multiple G runs were obtained by PCR site directed mutagenesis.

    Polymerase Chain Reaction:

    Article Title: Complex splicing control of the human Thrombopoietin gene by intronic G runs
    Article Snippet: .. The PCR product was EcoRV-XhoI cloned in pcDNA expression vector (Invitrogen) so generating the construct IVS2-wt (Figure 1c). .. Subsequently, the constructs carrying disruption of single or multiple G runs were obtained by PCR site directed mutagenesis.

    Transformation Assay:

    Article Title: A Role for Ectophosphatase in Xenobiotic Resistance
    Article Snippet: .. The complete 4-kb cDNA fragment was cut out with StuI (which cleaves in the leader sequence) and SalI (which cuts in the polylinker of pUC12 downstream of the cDNA insert) and inserted into a EcoRV-XhoI–cleaved pcDNA I/Amp vector (Invitrogen, Carlsbad, CA); from this, it was cut out again as a BamHI fragment and finally inserted in the correct orientation into the BamHI site of the yeast expression vector pvt101U ( ). pvt101 containing MDR1 and pvt101 alone were each independently transformed into Saccharomyces cerevisiae INVSC1 (MATα his3- Δ 1 leu2 trp1-289 ura3-52 ), YMR4 (MATα his3-11,15 leu2-3 112ura3 Δ 5 can Res pho5,3 :: ura3 Δ 1 ), YPH499 ( ura3-5 2 leu2- Δ 1 his3 - Δ200 ade2-101och lys2-801a trp1-Δ63 ), and YKKA-7 ( ura3-52 leu2- Δ 1 his3-Δ200 ade2-101och lys2-801a Δpdr5 :: TRP1 ) by a polyethylene glycol–lithium acetate procedure ( ) and selected on uracil dropout medium. .. Yeast were grown at 30°C under conditions of constant selection for uracil auxotrophy.

    Plasmid Preparation:

    Article Title: A Role for Ectophosphatase in Xenobiotic Resistance
    Article Snippet: .. The complete 4-kb cDNA fragment was cut out with StuI (which cleaves in the leader sequence) and SalI (which cuts in the polylinker of pUC12 downstream of the cDNA insert) and inserted into a EcoRV-XhoI–cleaved pcDNA I/Amp vector (Invitrogen, Carlsbad, CA); from this, it was cut out again as a BamHI fragment and finally inserted in the correct orientation into the BamHI site of the yeast expression vector pvt101U ( ). pvt101 containing MDR1 and pvt101 alone were each independently transformed into Saccharomyces cerevisiae INVSC1 (MATα his3- Δ 1 leu2 trp1-289 ura3-52 ), YMR4 (MATα his3-11,15 leu2-3 112ura3 Δ 5 can Res pho5,3 :: ura3 Δ 1 ), YPH499 ( ura3-5 2 leu2- Δ 1 his3 - Δ200 ade2-101och lys2-801a trp1-Δ63 ), and YKKA-7 ( ura3-52 leu2- Δ 1 his3-Δ200 ade2-101och lys2-801a Δpdr5 :: TRP1 ) by a polyethylene glycol–lithium acetate procedure ( ) and selected on uracil dropout medium. .. Yeast were grown at 30°C under conditions of constant selection for uracil auxotrophy.

    Article Title: Complex splicing control of the human Thrombopoietin gene by intronic G runs
    Article Snippet: .. The PCR product was EcoRV-XhoI cloned in pcDNA expression vector (Invitrogen) so generating the construct IVS2-wt (Figure 1c). .. Subsequently, the constructs carrying disruption of single or multiple G runs were obtained by PCR site directed mutagenesis.

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    Thermo Fisher ecori xhoi restriction sites
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