ecorv restriction sites  (New England Biolabs)


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  • 99
    Name:
    FseI
    Description:
    FseI 500 units
    Catalog Number:
    r0588l
    Price:
    302
    Size:
    500 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs ecorv restriction sites
    FseI
    FseI 500 units
    https://www.bioz.com/result/ecorv restriction sites/product/New England Biolabs
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ecorv restriction sites - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Immunization with HIV-1 envelope T20-encoding DNA vaccines elicits cross-clade neutralizing antibody responses
    Article Snippet: .. Minicircle MC-T20 ( ): The nucleotide (nt) sequence for the T20 peptide with a secretion signal (ss) from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant proteins (Immunomedics Inc., Morris Plains, NJ, USA) was cloned into the pCMV-T20 plasmid (see pCMV-EnvABC ) using Fse I and EcoRV restriction sites, by enzymes from New England Biolabs (R0588S and R0195S, NEB). .. PCR was performed to amplify the ss-T20 gene fragment (Eurofins Genomics, DE).

    Purification:

    Article Title: Molecular Characterization of Brucella abortus Chromosome II Recombination
    Article Snippet: .. The cohesive-end generating restriction endonuclease enzymes (REE) Nsi I, Sph I, or Fse I (New England Biolabs) were used to digest 2308 genomic DNA in 50 μl containing 5 μl of 10× REE buffer, 1,200 ng of DNA, 5 U of REE, and 0.5 μl of 10-μg/ml bovine serum albumin (for Fse I digestions only) for 4 h at 37°C and then purified by a nucleotide column (Qiagen). .. Each DNA digest was ligated to the adaptors at room temperature for 5 min in a reaction mixture of 100 μl containing (e.g., for Nsi I digests) 50 μl of Quick ligation buffer, 3.5 μl of primer PAOK-NsiI-12 (20 pM/μl), 7 μl of primer PAOKARA-24 (20 pM/μl) (supplementary Table B), and 5 μl of Quick T4 DNA ligase (New England Biolabs).

    Electrophoresis:

    Article Title: Construction of Gpm6a/ReelinGFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells
    Article Snippet: .. The vector was digested with Not I and Fse I (New England Biolabs), and electrophoresis was performed with agarose gels (Takara, Osaka, Japan). .. The large fragment of GFPCreERT2 was extracted from the gel with a quick gel extraction kit (Qiagen).

    Article Title: senX3-independent contribution of regX3 to Mycobacterium tuberculosis virulence
    Article Snippet: .. Genomic DNA from senX3 ::Tn and regX3 ::Tn complement candidates was digested with FseI and SphI-HF (New England Biolabs), respectively, and electrophoresis was performed on 1% agarose gels. .. Following denaturation and neutralization, the gels were transferred onto positively charged nylon membranes (GE Healthcare).

    Polymerase Chain Reaction:

    Article Title: Designing and Cloning Molecular Constructs to Knock Out N-Acetylglucosamine Phosphatidylinositol De-N-Acetylase (GPI12) Gene in Leishmania major (MRHO/IR/75/ER)
    Article Snippet: .. The PCR fragments were cut by Fse I and Bsr gI enzymes (New England Biolabs, UK). .. The plasmid pGEM-B1 contains sequences of flank areas, which preform PCR by primer M13.

    Sequencing:

    Article Title: Immunization with HIV-1 envelope T20-encoding DNA vaccines elicits cross-clade neutralizing antibody responses
    Article Snippet: .. Minicircle MC-T20 ( ): The nucleotide (nt) sequence for the T20 peptide with a secretion signal (ss) from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant proteins (Immunomedics Inc., Morris Plains, NJ, USA) was cloned into the pCMV-T20 plasmid (see pCMV-EnvABC ) using Fse I and EcoRV restriction sites, by enzymes from New England Biolabs (R0588S and R0195S, NEB). .. PCR was performed to amplify the ss-T20 gene fragment (Eurofins Genomics, DE).

    Recombinant:

    Article Title: Immunization with HIV-1 envelope T20-encoding DNA vaccines elicits cross-clade neutralizing antibody responses
    Article Snippet: .. Minicircle MC-T20 ( ): The nucleotide (nt) sequence for the T20 peptide with a secretion signal (ss) from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant proteins (Immunomedics Inc., Morris Plains, NJ, USA) was cloned into the pCMV-T20 plasmid (see pCMV-EnvABC ) using Fse I and EcoRV restriction sites, by enzymes from New England Biolabs (R0588S and R0195S, NEB). .. PCR was performed to amplify the ss-T20 gene fragment (Eurofins Genomics, DE).

    Plasmid Preparation:

    Article Title: Construction of Gpm6a/ReelinGFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells
    Article Snippet: .. The vector was digested with Not I and Fse I (New England Biolabs), and electrophoresis was performed with agarose gels (Takara, Osaka, Japan). .. The large fragment of GFPCreERT2 was extracted from the gel with a quick gel extraction kit (Qiagen).

    Article Title: Immunization with HIV-1 envelope T20-encoding DNA vaccines elicits cross-clade neutralizing antibody responses
    Article Snippet: .. Minicircle MC-T20 ( ): The nucleotide (nt) sequence for the T20 peptide with a secretion signal (ss) from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant proteins (Immunomedics Inc., Morris Plains, NJ, USA) was cloned into the pCMV-T20 plasmid (see pCMV-EnvABC ) using Fse I and EcoRV restriction sites, by enzymes from New England Biolabs (R0588S and R0195S, NEB). .. PCR was performed to amplify the ss-T20 gene fragment (Eurofins Genomics, DE).

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  • 90
    New England Biolabs ecorv restriction site
    Ecorv Restriction Site, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecorv restriction site/product/New England Biolabs
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ecorv restriction site - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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