ecorv restriction sites  (New England Biolabs)


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  • 99
    Name:
    EcoRV
    Description:
    EcoRV 20 000 units
    Catalog Number:
    R0195L
    Price:
    249
    Category:
    Restriction Enzymes
    Size:
    20 000 units
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    Structured Review

    New England Biolabs ecorv restriction sites
    EcoRV
    EcoRV 20 000 units
    https://www.bioz.com/result/ecorv restriction sites/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecorv restriction sites - by Bioz Stars, 2021-07
    99/100 stars

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    Related Articles

    Southern Blot:

    Article Title: Medicago truncatula contains a second gene encoding a plastid located glutamine synthetase exclusively expressed in developing seeds
    Article Snippet: All Nucleic acids were quantified using a Nanodrop spectrophotometer (Thermo scientific). .. Southern blot analysis 20 μg of genomic M. truncatula DNA and 5μg of BAC mth2-53e90 DNA were digested with EcoRV , NcoI and BglII (New England Biolabs, UK). .. DNA fragments were separated on a 0.8% agarose gel and blotted onto Amersham Hybond™-N+ Nylon membranes (GE healthcare).

    BAC Assay:

    Article Title: Medicago truncatula contains a second gene encoding a plastid located glutamine synthetase exclusively expressed in developing seeds
    Article Snippet: All Nucleic acids were quantified using a Nanodrop spectrophotometer (Thermo scientific). .. Southern blot analysis 20 μg of genomic M. truncatula DNA and 5μg of BAC mth2-53e90 DNA were digested with EcoRV , NcoI and BglII (New England Biolabs, UK). .. DNA fragments were separated on a 0.8% agarose gel and blotted onto Amersham Hybond™-N+ Nylon membranes (GE healthcare).

    Purification:

    Article Title: Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase
    Article Snippet: The extracted genomic DNA was used as a PCR template to specifically amplify a 2-kb genomic region covering the entire donor, using primers p3 and p4. .. A portion (1 μg) of each purified amplicon was digested with 10 U EcoRV in NEBuffer 3.1 at 37°C for 3 h, for the purpose of distinguishing the edited and unedited amplicon by gel electrophoresis. ..

    Amplification:

    Article Title: Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase
    Article Snippet: The extracted genomic DNA was used as a PCR template to specifically amplify a 2-kb genomic region covering the entire donor, using primers p3 and p4. .. A portion (1 μg) of each purified amplicon was digested with 10 U EcoRV in NEBuffer 3.1 at 37°C for 3 h, for the purpose of distinguishing the edited and unedited amplicon by gel electrophoresis. ..

    Nucleic Acid Electrophoresis:

    Article Title: Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase
    Article Snippet: The extracted genomic DNA was used as a PCR template to specifically amplify a 2-kb genomic region covering the entire donor, using primers p3 and p4. .. A portion (1 μg) of each purified amplicon was digested with 10 U EcoRV in NEBuffer 3.1 at 37°C for 3 h, for the purpose of distinguishing the edited and unedited amplicon by gel electrophoresis. ..

    Sequencing:

    Article Title: Inferring the annual migration patterns of fall armyworm (Lepidoptera: Noctuidae) in the United States from mitochondrial haplotypes
    Article Snippet: Amplification of the COI region used the primer pair COI-893F (5′-CACGAGCATATTTTACATCWGCA-3′) and COI-1303R (5′-CAGGATAGTCAGAATATCGACG-3′) to produce a 410-bp fragment (Integrated DNA Technologies, Coralville, IA). .. Strain identification and DNA sequence analysis In each PCR, reaction mix (30 µL) was added 5 units of the restriction enzyme EcoRV (New England Biolabs) and 4 µL of the manufacturer recommended 10× restriction enzyme buffer (final volume taken to 40 µL with water). .. Restriction digests were incubated at 37°C 1–3 h. For each reaction, 6 µL of 6× gel loading buffer was added and the entire sample run on a 1.8% agarose horizontal gel containing GelRed per manufacturer's instructions (Biotium, Hayward, CA) in 0.5× Tris-borate buffer (TBE, 45 mM Tris base, 45 mM boric acid, 1 mM EDTA pH 8.0).

    Polymerase Chain Reaction:

    Article Title: Inferring the annual migration patterns of fall armyworm (Lepidoptera: Noctuidae) in the United States from mitochondrial haplotypes
    Article Snippet: Amplification of the COI region used the primer pair COI-893F (5′-CACGAGCATATTTTACATCWGCA-3′) and COI-1303R (5′-CAGGATAGTCAGAATATCGACG-3′) to produce a 410-bp fragment (Integrated DNA Technologies, Coralville, IA). .. Strain identification and DNA sequence analysis In each PCR, reaction mix (30 µL) was added 5 units of the restriction enzyme EcoRV (New England Biolabs) and 4 µL of the manufacturer recommended 10× restriction enzyme buffer (final volume taken to 40 µL with water). .. Restriction digests were incubated at 37°C 1–3 h. For each reaction, 6 µL of 6× gel loading buffer was added and the entire sample run on a 1.8% agarose horizontal gel containing GelRed per manufacturer's instructions (Biotium, Hayward, CA) in 0.5× Tris-borate buffer (TBE, 45 mM Tris base, 45 mM boric acid, 1 mM EDTA pH 8.0).

    Isolation:

    Article Title: Regulated Expression of the Beta2-Toxin Gene (cpb2) in Clostridium perfringens Type A Isolates from Horses with Gastrointestinal Diseases
    Article Snippet: A 318-bp digoxigenin (DIG)-labeled cpb2 -specific DNA probe was prepared by using a two-step PCR amplification method as previously described ( ). .. Isolated C. perfringens DNA samples, prepared as previously described ( , ), were digested with EcoRV or HpaI (New England Biolabs), separated by electrophoresis on 1% agarose gels, and Southern transferred. .. The blots were hybridized with the DIG-labeled cpb2 probe, which was then detected using a DIG chemiluminescence detection system utilizing CSPD [disodium 3-(4-methoxyspiro{1,2-dioxetane-3,2′-(5-chloro)tricyclo[3,3.1.13.7]decane}-4-yl)phenyl phosphate] ready-to-use substrate (Roche) as described earlier ( ).

    Electrophoresis:

    Article Title: Regulated Expression of the Beta2-Toxin Gene (cpb2) in Clostridium perfringens Type A Isolates from Horses with Gastrointestinal Diseases
    Article Snippet: A 318-bp digoxigenin (DIG)-labeled cpb2 -specific DNA probe was prepared by using a two-step PCR amplification method as previously described ( ). .. Isolated C. perfringens DNA samples, prepared as previously described ( , ), were digested with EcoRV or HpaI (New England Biolabs), separated by electrophoresis on 1% agarose gels, and Southern transferred. .. The blots were hybridized with the DIG-labeled cpb2 probe, which was then detected using a DIG chemiluminescence detection system utilizing CSPD [disodium 3-(4-methoxyspiro{1,2-dioxetane-3,2′-(5-chloro)tricyclo[3,3.1.13.7]decane}-4-yl)phenyl phosphate] ready-to-use substrate (Roche) as described earlier ( ).

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  • 99
    New England Biolabs ecorv restriction sites
    Ecorv Restriction Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecorv restriction sites/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecorv restriction sites - by Bioz Stars, 2021-07
    99/100 stars
      Buy from Supplier

    86
    New England Biolabs ecorv restriction site
    Ecorv Restriction Site, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecorv restriction site/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecorv restriction site - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

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