ecorv restriction site  (New England Biolabs)


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    Structured Review

    New England Biolabs ecorv restriction site
    Ecorv Restriction Site, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecorv restriction site/product/New England Biolabs
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ecorv restriction site - by Bioz Stars, 2020-08
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Endogenous Enterobacteriaceae underlie variation in susceptibility to Salmonella infection
    Article Snippet: .. The fragment was then cloned into the EcoRV restriction site of the low copy number plasmid pWSK129 to generate the plasmid pBMM17 via Gibson Assembly Master Mix (NEB). ..

    Article Title: Reconstitution of the Human tRNA Splicing Endonuclease Complex: insight into the regulation of pre-tRNA cleavage
    Article Snippet: .. Full-length tRNA genes were synthesized and cloned into pUC19 vectors by Genescript, genes were flanked by a 5’ T7 promoter and a 3’ EcoRV restriction site (sequences are available in ). tRNA containing plasmids were linearized using restriction digestion and tRNAs were in vitro transcribed overnight using the HiScribe T7 transcription kit (NEB) per the manufacturer’s protocol. .. RNA was then either cleaned up using the RNAeasy kit (Qiagen) following DNase digestion (Qiagen) or run on TBE-Urea gels (Novex), visualized by UV backlighting on a TLC plate, and purified from the gel.

    Article Title: Enterovirus Pathogenesis Requires the Host Methyltransferase SETD3
    Article Snippet: .. SETD3 were PCR amplified and subsequently cloned into the EcoRV restriction site of pBIND by Gibson Assembly (New England BioLabs). .. 2AC107A were PCR amplified and subsequently cloned into the EcoRV restriction sites of pACT by Gibson Assembly (New England BioLabs).

    Amplification:

    Article Title: Enterovirus Pathogenesis Requires the Host Methyltransferase SETD3
    Article Snippet: .. SETD3 were PCR amplified and subsequently cloned into the EcoRV restriction site of pBIND by Gibson Assembly (New England BioLabs). .. 2AC107A were PCR amplified and subsequently cloned into the EcoRV restriction sites of pACT by Gibson Assembly (New England BioLabs).

    In Vitro:

    Article Title: Reconstitution of the Human tRNA Splicing Endonuclease Complex: insight into the regulation of pre-tRNA cleavage
    Article Snippet: .. Full-length tRNA genes were synthesized and cloned into pUC19 vectors by Genescript, genes were flanked by a 5’ T7 promoter and a 3’ EcoRV restriction site (sequences are available in ). tRNA containing plasmids were linearized using restriction digestion and tRNAs were in vitro transcribed overnight using the HiScribe T7 transcription kit (NEB) per the manufacturer’s protocol. .. RNA was then either cleaned up using the RNAeasy kit (Qiagen) following DNase digestion (Qiagen) or run on TBE-Urea gels (Novex), visualized by UV backlighting on a TLC plate, and purified from the gel.

    Synthesized:

    Article Title: Reconstitution of the Human tRNA Splicing Endonuclease Complex: insight into the regulation of pre-tRNA cleavage
    Article Snippet: .. Full-length tRNA genes were synthesized and cloned into pUC19 vectors by Genescript, genes were flanked by a 5’ T7 promoter and a 3’ EcoRV restriction site (sequences are available in ). tRNA containing plasmids were linearized using restriction digestion and tRNAs were in vitro transcribed overnight using the HiScribe T7 transcription kit (NEB) per the manufacturer’s protocol. .. RNA was then either cleaned up using the RNAeasy kit (Qiagen) following DNase digestion (Qiagen) or run on TBE-Urea gels (Novex), visualized by UV backlighting on a TLC plate, and purified from the gel.

    Low Copy Number:

    Article Title: Endogenous Enterobacteriaceae underlie variation in susceptibility to Salmonella infection
    Article Snippet: .. The fragment was then cloned into the EcoRV restriction site of the low copy number plasmid pWSK129 to generate the plasmid pBMM17 via Gibson Assembly Master Mix (NEB). ..

    Polymerase Chain Reaction:

    Article Title: Enterovirus Pathogenesis Requires the Host Methyltransferase SETD3
    Article Snippet: .. SETD3 were PCR amplified and subsequently cloned into the EcoRV restriction site of pBIND by Gibson Assembly (New England BioLabs). .. 2AC107A were PCR amplified and subsequently cloned into the EcoRV restriction sites of pACT by Gibson Assembly (New England BioLabs).

    Plasmid Preparation:

    Article Title: Endogenous Enterobacteriaceae underlie variation in susceptibility to Salmonella infection
    Article Snippet: .. The fragment was then cloned into the EcoRV restriction site of the low copy number plasmid pWSK129 to generate the plasmid pBMM17 via Gibson Assembly Master Mix (NEB). ..

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  • 90
    New England Biolabs ecorv restriction site
    Ecorv Restriction Site, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecorv restriction site/product/New England Biolabs
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ecorv restriction site - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    90
    New England Biolabs ecori restriction site
    Ecori Restriction Site, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecori restriction site/product/New England Biolabs
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ecori restriction site - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

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