ecori xhoi digested pcr3 1  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ecori xhoi digested pcr3 1
    Ecori Xhoi Digested Pcr3 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecori xhoi digested pcr3 1/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecori xhoi digested pcr3 1 - by Bioz Stars, 2020-04
    91/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Altered stoichiometry and nuclear delocalization of NonO and PSF promote cellular senescence
    Article Snippet: Plasmids pCR3.1-HA-NonO was constructed by excising full-length HA-NonO from pBS-HA-NonO1.4 vector with EcoRI/XhoI, and ligating it to EcoRI/XhoI digested pCR3.1 (Invitrogen). pBI-NonO2.4 was generated by excising full length NonO from p5.7NonO with XhoI and ligating it to SalI digested pBI (Clontech). .. To construct GFP-NonO and all the other mutants, NonO fragments were generated by PCR using a 5′ primer containing a XhoI restriction site and a 3′ primer containing an EcoRI restriction site and template p5.7NonO.

    Clone Assay:

    Article Title: Altered stoichiometry and nuclear delocalization of NonO and PSF promote cellular senescence
    Article Snippet: Plasmids pCR3.1-HA-NonO was constructed by excising full-length HA-NonO from pBS-HA-NonO1.4 vector with EcoRI/XhoI, and ligating it to EcoRI/XhoI digested pCR3.1 (Invitrogen). pBI-NonO2.4 was generated by excising full length NonO from p5.7NonO with XhoI and ligating it to SalI digested pBI (Clontech). .. GFP-NonO and deletion mutants were created by cloning a NonO cDNA into the pEGFP-C1 vector (Clontech).

    Generated:

    Article Title: Altered stoichiometry and nuclear delocalization of NonO and PSF promote cellular senescence
    Article Snippet: .. Plasmids pCR3.1-HA-NonO was constructed by excising full-length HA-NonO from pBS-HA-NonO1.4 vector with EcoRI/XhoI, and ligating it to EcoRI/XhoI digested pCR3.1 (Invitrogen). pBI-NonO2.4 was generated by excising full length NonO from p5.7NonO with XhoI and ligating it to SalI digested pBI (Clontech). .. GFP-NonO and deletion mutants were created by cloning a NonO cDNA into the pEGFP-C1 vector (Clontech).

    Construct:

    Article Title: Altered stoichiometry and nuclear delocalization of NonO and PSF promote cellular senescence
    Article Snippet: .. Plasmids pCR3.1-HA-NonO was constructed by excising full-length HA-NonO from pBS-HA-NonO1.4 vector with EcoRI/XhoI, and ligating it to EcoRI/XhoI digested pCR3.1 (Invitrogen). pBI-NonO2.4 was generated by excising full length NonO from p5.7NonO with XhoI and ligating it to SalI digested pBI (Clontech). .. GFP-NonO and deletion mutants were created by cloning a NonO cDNA into the pEGFP-C1 vector (Clontech).

    Plasmid Preparation:

    Article Title: Altered stoichiometry and nuclear delocalization of NonO and PSF promote cellular senescence
    Article Snippet: .. Plasmids pCR3.1-HA-NonO was constructed by excising full-length HA-NonO from pBS-HA-NonO1.4 vector with EcoRI/XhoI, and ligating it to EcoRI/XhoI digested pCR3.1 (Invitrogen). pBI-NonO2.4 was generated by excising full length NonO from p5.7NonO with XhoI and ligating it to SalI digested pBI (Clontech). .. GFP-NonO and deletion mutants were created by cloning a NonO cDNA into the pEGFP-C1 vector (Clontech).