ecori xbai sites  (Thermo Fisher)


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    Name:
    EcoRI 10 U µL
    Description:
    5 G ↓A A T T C 3 3 C T T A A ↑G 5 Thermo Scientific EcoRI restriction enzyme recognizes G AATTC sites and cuts best at 37°C in its own unique buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that sent in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    Catalog Number:
    er0271
    Price:
    None
    Category:
    Proteins Enzymes Peptides
    Applications:
    Cloning|Restriction Enzyme Cloning
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    Structured Review

    Thermo Fisher ecori xbai sites
    5 G ↓A A T T C 3 3 C T T A A ↑G 5 Thermo Scientific EcoRI restriction enzyme recognizes G AATTC sites and cuts best at 37°C in its own unique buffer See Reaction Conditions for Restriction Enzymes for a table of enzyme activity conditions for double digestion and heat inactivation for this and other restriction enzymes Note Also available as a FastDigest enzyme for rapid DNA digestion Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes optimized to work in one of the buffers of the Five Buffer System In addition the universal Tango buffer is provided for convenience in double digestions All of the enzymes exhibit 100 activity in the recommended buffer and reaction conditions To ensure consistent performance Thermo Scientific restriction enzyme reaction buffers contain premixed BSA which enhances the stability of many enzymes and binds contaminants that sent in DNA preparations Features• Superior quality stringent quality control and industry leading manufacturing process• Convenient color coded Five Buffer System• Includes universal Tango buffer for double digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificitiesApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern blotting• Restriction fragment length polymorphism RFLP • SNPNote For methylation sensitivity refer to product specifications
    https://www.bioz.com/result/ecori xbai sites/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecori xbai sites - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Expressing:

    Article Title: Plasmodium vivax Ookinete Surface Protein Pvs25 Linked to Cholera Toxin B Subunit Induces Potent Transmission-Blocking Immunity by Intranasal as Well as Subcutaneous Immunization ▿
    Article Snippet: The DNA fragment was amplified by using Vent DNA polymerase (New England Biolabs, Beverly, MA), was purified by using a PCR Purification Kit (Qiagen, Inc., Valencia, CA), and then digested with EcoRI. .. The fragment was subcloned into the SnaBI and EcoRI sites of the P. pastoris expression vector pPIC9K (Life Technologies, Carlsbad, CA) to construct the plasmid pPvs25H, which was designed to express an α-factor signal-Pvs25-hexahistidine fusion protein (see Fig. ). .. P. pastoris recombination was performed according to the manufacturer's instructions (Life Technologies).

    Article Title: Cloning of a Recombinant Plasmid Encoding Thiol-Specific Antioxidant Antigen (TSA) Gene of Leishmania majorand Expression in the Chinese Hamster Ovary Cell Line
    Article Snippet: The PCR product was then cloned into the pTZ57R/T vector and transformed into Escherichia coli (TG1 strain) ( ). .. To subclone TSA into the pcDNA3 plasmid, the gene was cloned with linkers to join it to the HindIII and EcoRI sites of pcDNA3 (Invitrogen, US) to produce the recombinant eukaryotic expression plasmid pcTSA. ..

    Plasmid Preparation:

    Article Title: Plasmodium vivax Ookinete Surface Protein Pvs25 Linked to Cholera Toxin B Subunit Induces Potent Transmission-Blocking Immunity by Intranasal as Well as Subcutaneous Immunization ▿
    Article Snippet: The DNA fragment was amplified by using Vent DNA polymerase (New England Biolabs, Beverly, MA), was purified by using a PCR Purification Kit (Qiagen, Inc., Valencia, CA), and then digested with EcoRI. .. The fragment was subcloned into the SnaBI and EcoRI sites of the P. pastoris expression vector pPIC9K (Life Technologies, Carlsbad, CA) to construct the plasmid pPvs25H, which was designed to express an α-factor signal-Pvs25-hexahistidine fusion protein (see Fig. ). .. P. pastoris recombination was performed according to the manufacturer's instructions (Life Technologies).

    Article Title: Regulation of p53 during senescence in normal human keratinocytes
    Article Snippet: .. To access the accuracy of the end joining activity, we cloned the PCR products from the ECoRI-linearized and ligated pCR2.1-TOPO plasmid into pcDNA3.1/V5-His TOPO plasmid (Invitrogen). .. The resulting ligated products were introduced into TOP10 cells (Invitrogen), and the single colony PCR was performed using the M13 primers (100 clones per samples).

    Article Title: Cloning of a Recombinant Plasmid Encoding Thiol-Specific Antioxidant Antigen (TSA) Gene of Leishmania majorand Expression in the Chinese Hamster Ovary Cell Line
    Article Snippet: The PCR product was then cloned into the pTZ57R/T vector and transformed into Escherichia coli (TG1 strain) ( ). .. To subclone TSA into the pcDNA3 plasmid, the gene was cloned with linkers to join it to the HindIII and EcoRI sites of pcDNA3 (Invitrogen, US) to produce the recombinant eukaryotic expression plasmid pcTSA. ..

    Article Title: Design, Construction and Immunogenicity Assessment of pEGFP-N1-KMP11-GP96 (Fusion) as a DNA Vaccine Candidate against Leishmania major Infection in BALB/c Mice
    Article Snippet: For confirmation, PCR amplifications were performed in these colonies using primers specific for KMP-11and NT-GP96 genes and colonies containing the recombinant plasmid were selected. .. Recombinant plasmids were extracted by Vivantis plasmid extraction kit and digested by Bgl II / EcoRI (for KMP-11), EcoRI / KpnI (for NT-GP96 ) restriction enzymes (Fermentas Co.®) for digestion confirmation. .. Subcloning of KMP-11and NT-GP96 in pEGFP-N1expression vector pJET- KMP-11, pJET- NT-GP96 and pEGFP-N1 were digested by Bgl II / EcoRI (for KMP-11), EcoRI / KpnI (for NT-GP96 ) restriction enzymes and the purified gene fragment of KMP-11and NT-GP96 KMP-11 ligated into digested pEGFPN1expression vector by using of T4 DNA ligase enzyme.

    Article Title: Multicopy blaOXA-58 Gene as a Source of High-Level Resistance to Carbapenems in Acinetobacter baumannii ▿
    Article Snippet: PCR products were ligated into the pCR2.1 vector (Invitrogen, Milan, Italy) and used to transform competent Escherichia coli INVαF′ cells (INVαF′ Chemically Competent Escherichia coli ; Invitrogen, Milan, Italy), and selection of the transformants was performed on LB agar plates containing ampicillin (100 μg/ml). .. SacI libraries were obtained from isolates 183 and 186, and EcoRI libraries were obtained from isolates 183, 186, and 193 in the pZErO-2 vector (Invitrogen, Milan, Italy). .. The libraries were used to transform competent E. coli DH5α cells (MAX Efficiency DH5α Chemically Competent Cells; Invitrogen, Milan, Italy), and selection of the transformants was performed on LB agar plates containing kanamycin (40 μg/ml), ampicillin (20 μg/ml), and 1 mM IPTG (isopropyl-β- d -thiogalactopyranoside).

    Article Title: Proteomics and Transcriptomics of BJAB Cells Expressing the Epstein-Barr Virus Noncoding RNAs EBER1 and EBER2
    Article Snippet: .. pCEP4 vector constructs To introduce more than one copy of the EcoRI-J fragment into the pCEP4 plasmid (Invitrogen), we took advantage of the BglII and HindIII restriction sites up- and downstream, respectively, of the CMV promoter and of a BamHI one downstream the HindIII site as described in . .. To make stable cell lines, we transfected BJAB cells with each of these plasmids by electroporating at 230 V with 10 μg of pCEP4 empty or containing the EcoRI-J fragment, plus a similar amount of a GFP-expressing plasmid (pGFP-MAX) to trace the transfection efficiency as described above before [ ].

    Construct:

    Article Title: Plasmodium vivax Ookinete Surface Protein Pvs25 Linked to Cholera Toxin B Subunit Induces Potent Transmission-Blocking Immunity by Intranasal as Well as Subcutaneous Immunization ▿
    Article Snippet: The DNA fragment was amplified by using Vent DNA polymerase (New England Biolabs, Beverly, MA), was purified by using a PCR Purification Kit (Qiagen, Inc., Valencia, CA), and then digested with EcoRI. .. The fragment was subcloned into the SnaBI and EcoRI sites of the P. pastoris expression vector pPIC9K (Life Technologies, Carlsbad, CA) to construct the plasmid pPvs25H, which was designed to express an α-factor signal-Pvs25-hexahistidine fusion protein (see Fig. ). .. P. pastoris recombination was performed according to the manufacturer's instructions (Life Technologies).

    Article Title: Proteomics and Transcriptomics of BJAB Cells Expressing the Epstein-Barr Virus Noncoding RNAs EBER1 and EBER2
    Article Snippet: .. pCEP4 vector constructs To introduce more than one copy of the EcoRI-J fragment into the pCEP4 plasmid (Invitrogen), we took advantage of the BglII and HindIII restriction sites up- and downstream, respectively, of the CMV promoter and of a BamHI one downstream the HindIII site as described in . .. To make stable cell lines, we transfected BJAB cells with each of these plasmids by electroporating at 230 V with 10 μg of pCEP4 empty or containing the EcoRI-J fragment, plus a similar amount of a GFP-expressing plasmid (pGFP-MAX) to trace the transfection efficiency as described above before [ ].

    Activity Assay:

    Article Title: Regulation of p53 during senescence in normal human keratinocytes
    Article Snippet: .. To access the accuracy of the end joining activity, we cloned the PCR products from the ECoRI-linearized and ligated pCR2.1-TOPO plasmid into pcDNA3.1/V5-His TOPO plasmid (Invitrogen). .. The resulting ligated products were introduced into TOP10 cells (Invitrogen), and the single colony PCR was performed using the M13 primers (100 clones per samples).

    Clone Assay:

    Article Title: Regulation of p53 during senescence in normal human keratinocytes
    Article Snippet: .. To access the accuracy of the end joining activity, we cloned the PCR products from the ECoRI-linearized and ligated pCR2.1-TOPO plasmid into pcDNA3.1/V5-His TOPO plasmid (Invitrogen). .. The resulting ligated products were introduced into TOP10 cells (Invitrogen), and the single colony PCR was performed using the M13 primers (100 clones per samples).

    Article Title: Cloning of a Recombinant Plasmid Encoding Thiol-Specific Antioxidant Antigen (TSA) Gene of Leishmania majorand Expression in the Chinese Hamster Ovary Cell Line
    Article Snippet: The PCR product was then cloned into the pTZ57R/T vector and transformed into Escherichia coli (TG1 strain) ( ). .. To subclone TSA into the pcDNA3 plasmid, the gene was cloned with linkers to join it to the HindIII and EcoRI sites of pcDNA3 (Invitrogen, US) to produce the recombinant eukaryotic expression plasmid pcTSA. ..

    Polymerase Chain Reaction:

    Article Title: Regulation of p53 during senescence in normal human keratinocytes
    Article Snippet: .. To access the accuracy of the end joining activity, we cloned the PCR products from the ECoRI-linearized and ligated pCR2.1-TOPO plasmid into pcDNA3.1/V5-His TOPO plasmid (Invitrogen). .. The resulting ligated products were introduced into TOP10 cells (Invitrogen), and the single colony PCR was performed using the M13 primers (100 clones per samples).

    Recombinant:

    Article Title: Cloning of a Recombinant Plasmid Encoding Thiol-Specific Antioxidant Antigen (TSA) Gene of Leishmania majorand Expression in the Chinese Hamster Ovary Cell Line
    Article Snippet: The PCR product was then cloned into the pTZ57R/T vector and transformed into Escherichia coli (TG1 strain) ( ). .. To subclone TSA into the pcDNA3 plasmid, the gene was cloned with linkers to join it to the HindIII and EcoRI sites of pcDNA3 (Invitrogen, US) to produce the recombinant eukaryotic expression plasmid pcTSA. ..

    Article Title: Design, Construction and Immunogenicity Assessment of pEGFP-N1-KMP11-GP96 (Fusion) as a DNA Vaccine Candidate against Leishmania major Infection in BALB/c Mice
    Article Snippet: For confirmation, PCR amplifications were performed in these colonies using primers specific for KMP-11and NT-GP96 genes and colonies containing the recombinant plasmid were selected. .. Recombinant plasmids were extracted by Vivantis plasmid extraction kit and digested by Bgl II / EcoRI (for KMP-11), EcoRI / KpnI (for NT-GP96 ) restriction enzymes (Fermentas Co.®) for digestion confirmation. .. Subcloning of KMP-11and NT-GP96 in pEGFP-N1expression vector pJET- KMP-11, pJET- NT-GP96 and pEGFP-N1 were digested by Bgl II / EcoRI (for KMP-11), EcoRI / KpnI (for NT-GP96 ) restriction enzymes and the purified gene fragment of KMP-11and NT-GP96 KMP-11 ligated into digested pEGFPN1expression vector by using of T4 DNA ligase enzyme.

    Introduce:

    Article Title: Proteomics and Transcriptomics of BJAB Cells Expressing the Epstein-Barr Virus Noncoding RNAs EBER1 and EBER2
    Article Snippet: .. pCEP4 vector constructs To introduce more than one copy of the EcoRI-J fragment into the pCEP4 plasmid (Invitrogen), we took advantage of the BglII and HindIII restriction sites up- and downstream, respectively, of the CMV promoter and of a BamHI one downstream the HindIII site as described in . .. To make stable cell lines, we transfected BJAB cells with each of these plasmids by electroporating at 230 V with 10 μg of pCEP4 empty or containing the EcoRI-J fragment, plus a similar amount of a GFP-expressing plasmid (pGFP-MAX) to trace the transfection efficiency as described above before [ ].

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  • 99
    Thermo Fisher ecori xbai sites
    Ecori Xbai Sites, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecori xbai sites/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecori xbai sites - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

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