ecori mspi  (New England Biolabs)


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  • 99
    Name:
    EcoRI
    Description:
    EcoRI 50 000 units
    Catalog Number:
    r0101l
    Price:
    249
    Size:
    50 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs ecori mspi
    EcoRI
    EcoRI 50 000 units
    https://www.bioz.com/result/ecori mspi/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ecori mspi - by Bioz Stars, 2020-07
    99/100 stars

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    Clone Assay:

    Article Title: Generation of recombinant Orf virus using an enhanced green fluorescent protein reporter gene as a selectable marker
    Article Snippet: .. The products were then cloned into vector pZIPPY-neo/gus [ ], which had been linearized with EcoRI and NotI (New England Biolabs, Ipswich, MA, USA) to generate the novel transfer vector pSPV-EGFP (Figure ). .. The plasmid was propagated in Escherichia coli strain Top10 (Invitrogen, Carlsbad, CA, USA).

    Amplification:

    Article Title: Morphological, Genome and Gene Expression Changes in Newly Induced Autopolyploid Chrysanthemum lavandulifolium (Fisch. ex Trautv.) Makino
    Article Snippet: .. Mthylation Sensitive Amplified Polymorphism Analysis The MSAP technique was applied to the DNA pools from three diploid lines and three tetraploid lines C. lavandulifolium They were digested with either EcoR I and Hpa II or EcoR I and Msp I (NEB) at 37 °C for 12 h. The digested fragments were ligated to 5 pmol EcoR I adaptor and 50 pmol Hpa II/Msp I adaptor by incubation with 4 U T4 DNA polymerase (NEB) at 16 °C for 4 has described for the AFLP method. ..

    Modification:

    Article Title: Increased retention of functional fusions to toxic genes in new two-hybrid libraries of the E. coli strain MG1655 and B. subtilis strain 168 genomes, prepared without passaging through E. coli
    Article Snippet: .. Construction of modified pB42 vectors, and preparation for ligation to insert DNA Plasmid pB42 was digested with XhoI and EcoRI to completion, and precipitated and digested with CIP (NEB). .. This vector was then split into three reactions, where three pairs of oligos were added to form the new multiple cloning sites.

    Ligation:

    Article Title: Increased retention of functional fusions to toxic genes in new two-hybrid libraries of the E. coli strain MG1655 and B. subtilis strain 168 genomes, prepared without passaging through E. coli
    Article Snippet: .. Construction of modified pB42 vectors, and preparation for ligation to insert DNA Plasmid pB42 was digested with XhoI and EcoRI to completion, and precipitated and digested with CIP (NEB). .. This vector was then split into three reactions, where three pairs of oligos were added to form the new multiple cloning sites.

    Polymerase Chain Reaction:

    Article Title: Restriction site detection in repetitive nuclear DNA sequences of Trypanosoma evansi for strain differentiation among different isolates
    Article Snippet: .. EcoRI, Eco91l, HindIII and PstI, for complete digestion with the recommended RE buffers in separate PCR tubes in the following reaction volume: 7 µl nuclease-free water, 10 µl DNA, 2 µl 10× RE buffer, 1 µl (10 U) EcoRI (New England Biolabs)/1 µl (10 U) Eco91l (Fermentas) / 1 µl (10 U) HindIII (Fermentas) / 1 µl (10 U) PstI (Fermentas). ..

    Incubation:

    Article Title: Morphological, Genome and Gene Expression Changes in Newly Induced Autopolyploid Chrysanthemum lavandulifolium (Fisch. ex Trautv.) Makino
    Article Snippet: .. Mthylation Sensitive Amplified Polymorphism Analysis The MSAP technique was applied to the DNA pools from three diploid lines and three tetraploid lines C. lavandulifolium They were digested with either EcoR I and Hpa II or EcoR I and Msp I (NEB) at 37 °C for 12 h. The digested fragments were ligated to 5 pmol EcoR I adaptor and 50 pmol Hpa II/Msp I adaptor by incubation with 4 U T4 DNA polymerase (NEB) at 16 °C for 4 has described for the AFLP method. ..

    Sequencing:

    Article Title: Characterization of untranslated regions of the salmonid alphavirus 3 (SAV3) genome and construction of a SAV3 based replicon
    Article Snippet: .. The authenticity of the plasmid construction was verified by EcoRI, AgeI and AscI (New England Biolabs) digestion (Fig. ) and by sequencing as previously described [ ]. .. This information indicated that eight substitutions were present in the RC coding region compared to the nucleotide sequence of passage 20 of the parental strain SAVH20/03 (Table ).

    Plasmid Preparation:

    Article Title: Deletion of the Clostridium thermocellum recA gene reveals that it is required for thermophilic plasmid replication but not plasmid integration at homologous DNA sequences
    Article Snippet: .. The colonies were picked into 10 mL LB with 50 μg/mL apramycin, and the plasmid DNA was extracted using a Miniprep kit (Qiagen, Valencia, CA, USA) and screened with restriction enzymes EcoRI and AvaI for pJGW92 and NcoI and AvaI for pJGW93 (NEB). .. The National Center for Biotechnology Information (NCBI) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to search for homologous proteins to known RecA, LexA, and DNA Pol V in the Clostridium thermocellum DSM 1313 genome.

    Article Title: Increased retention of functional fusions to toxic genes in new two-hybrid libraries of the E. coli strain MG1655 and B. subtilis strain 168 genomes, prepared without passaging through E. coli
    Article Snippet: .. Construction of modified pB42 vectors, and preparation for ligation to insert DNA Plasmid pB42 was digested with XhoI and EcoRI to completion, and precipitated and digested with CIP (NEB). .. This vector was then split into three reactions, where three pairs of oligos were added to form the new multiple cloning sites.

    Article Title: Generation of recombinant Orf virus using an enhanced green fluorescent protein reporter gene as a selectable marker
    Article Snippet: .. The products were then cloned into vector pZIPPY-neo/gus [ ], which had been linearized with EcoRI and NotI (New England Biolabs, Ipswich, MA, USA) to generate the novel transfer vector pSPV-EGFP (Figure ). .. The plasmid was propagated in Escherichia coli strain Top10 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Characterization of untranslated regions of the salmonid alphavirus 3 (SAV3) genome and construction of a SAV3 based replicon
    Article Snippet: .. The authenticity of the plasmid construction was verified by EcoRI, AgeI and AscI (New England Biolabs) digestion (Fig. ) and by sequencing as previously described [ ]. .. This information indicated that eight substitutions were present in the RC coding region compared to the nucleotide sequence of passage 20 of the parental strain SAVH20/03 (Table ).

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  • 85
    New England Biolabs ecori plus mspi
    Ecori Plus Mspi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecori plus mspi/product/New England Biolabs
    Average 85 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    ecori plus mspi - by Bioz Stars, 2020-07
    85/100 stars
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