ecor v sal i  (Millipore)

 
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    Name:
    EcoR I
    Description:
    EcoR I recognizes the sequence G↓ A ATT C and generates fragments with 5 cohesive termini EcoR I is inhibited if N6 methyladenine occurs at either or both A residues in the recognition site it is also inhibited if 5 methylcytosine occurs at the site indicated EcoR I is an isoschizomer to Rsr I
    Catalog Number:
    ECORI-RO
    Price:
    None
    Applications:
    EcoR I from Escherichia coli BS5 has been used to digest bacterial DNA.
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    Structured Review

    Millipore ecor v sal i
    EcoR I recognizes the sequence G↓ A ATT C and generates fragments with 5 cohesive termini EcoR I is inhibited if N6 methyladenine occurs at either or both A residues in the recognition site it is also inhibited if 5 methylcytosine occurs at the site indicated EcoR I is an isoschizomer to Rsr I
    https://www.bioz.com/result/ecor v sal i/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecor v sal i - by Bioz Stars, 2021-07
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Gene Silencing by RNA Interference in the White Rot Fungus Phanerochaete chrysosporium ▿
    Article Snippet: A 642-bp fragment corresponding to the MnSOD1 gene's 3′ untranslated region was amplified by PCR from genomic DNA with primers AM65 and AM59 to add a BamHI and an EcoRI restriction site, respectively (Table ). .. The PCR fragments were digested and ligated into the PstI and EcoRI sites of plasmid pBar3.8, containing the selectable marker gene bar for resistance to the herbicide phosphinothricin (PPT; Sigma-Aldrich, Rehovot, Israel) ( ). .. A new plasmid, designated pMSC, was obtained (Fig. ).

    Article Title: Identification and characterization of duck plague virus glycoprotein C gene and gene product
    Article Snippet: The amplified PCR product was cloned into a T/A cloning vector pMD18-T (TaKaRa), generating a recombinant cloning plasmid pMD18-T/gC (Figure ). .. After verified by PCR, restriction analysis and DNA sequencing (TaKaRa), the gC gene fragment, which was obtained by digestion of pMD18-T/gC with EcoRI and XhoI, was ligated into prokaryotic vector pET32a(+) (Novagen) (Figure ), which was digested previously with the same restriction enzymes. .. The recombinant plasmid, named pET32a-gC, was confirmed by PCR, restriction enzyme digestion and DNA sequencing (TaKaRa).

    Article Title: A Novel Gene, Encoding 6-Hydroxy-3-Succinoylpyridine Hydroxylase, Involved in Nicotine Degradation by Pseudomonas putida Strain S16 ▿ Strain S16 ▿ ‡
    Article Snippet: .. The PCR product of hsp was digested with EcoRI and XhoI and ligated into pET-27b(+) (Novagen). .. Transformed E. coli BL21(DE3) cells with hsp were incubated in 500-ml flasks\chatn with 100 ml of LB medium containing 100 mg of kanamycin liter−1 until an optical density at 600 nm of 0.5 was reached.

    Plasmid Preparation:

    Article Title: Gene Silencing by RNA Interference in the White Rot Fungus Phanerochaete chrysosporium ▿
    Article Snippet: A 642-bp fragment corresponding to the MnSOD1 gene's 3′ untranslated region was amplified by PCR from genomic DNA with primers AM65 and AM59 to add a BamHI and an EcoRI restriction site, respectively (Table ). .. The PCR fragments were digested and ligated into the PstI and EcoRI sites of plasmid pBar3.8, containing the selectable marker gene bar for resistance to the herbicide phosphinothricin (PPT; Sigma-Aldrich, Rehovot, Israel) ( ). .. A new plasmid, designated pMSC, was obtained (Fig. ).

    Article Title: Identification and characterization of duck plague virus glycoprotein C gene and gene product
    Article Snippet: The amplified PCR product was cloned into a T/A cloning vector pMD18-T (TaKaRa), generating a recombinant cloning plasmid pMD18-T/gC (Figure ). .. After verified by PCR, restriction analysis and DNA sequencing (TaKaRa), the gC gene fragment, which was obtained by digestion of pMD18-T/gC with EcoRI and XhoI, was ligated into prokaryotic vector pET32a(+) (Novagen) (Figure ), which was digested previously with the same restriction enzymes. .. The recombinant plasmid, named pET32a-gC, was confirmed by PCR, restriction enzyme digestion and DNA sequencing (TaKaRa).

    Article Title: Cloning, Expression, and Purification of a New Antibacterial Substance Gene From Larvae ofMusca domestica(Diptera: Muscidae)
    Article Snippet: The generated sequences were analyzed for similarity with other known sequences using the BLAST programs at the National Center for Biotechnology Information ( www.ncbi.nlm.gov/blast ). .. Construction of Expression Vector and Recombinant Protein Expression The recombinant plasmid pMD18T-AS566 was digested with EcoRI and XhoI enzymes, and ligated into the EcoRI- or XhoI-digested expression vector, pET-30a(+) (Millipore). .. The pET-30a-566 plasmid was transformed intoE. coli BL21 (DE3) cells for the expression of the AS566 protein.

    Marker:

    Article Title: Gene Silencing by RNA Interference in the White Rot Fungus Phanerochaete chrysosporium ▿
    Article Snippet: A 642-bp fragment corresponding to the MnSOD1 gene's 3′ untranslated region was amplified by PCR from genomic DNA with primers AM65 and AM59 to add a BamHI and an EcoRI restriction site, respectively (Table ). .. The PCR fragments were digested and ligated into the PstI and EcoRI sites of plasmid pBar3.8, containing the selectable marker gene bar for resistance to the herbicide phosphinothricin (PPT; Sigma-Aldrich, Rehovot, Israel) ( ). .. A new plasmid, designated pMSC, was obtained (Fig. ).

    DNA Sequencing:

    Article Title: Identification and characterization of duck plague virus glycoprotein C gene and gene product
    Article Snippet: The amplified PCR product was cloned into a T/A cloning vector pMD18-T (TaKaRa), generating a recombinant cloning plasmid pMD18-T/gC (Figure ). .. After verified by PCR, restriction analysis and DNA sequencing (TaKaRa), the gC gene fragment, which was obtained by digestion of pMD18-T/gC with EcoRI and XhoI, was ligated into prokaryotic vector pET32a(+) (Novagen) (Figure ), which was digested previously with the same restriction enzymes. .. The recombinant plasmid, named pET32a-gC, was confirmed by PCR, restriction enzyme digestion and DNA sequencing (TaKaRa).

    Expressing:

    Article Title: Cloning, Expression, and Purification of a New Antibacterial Substance Gene From Larvae ofMusca domestica(Diptera: Muscidae)
    Article Snippet: The generated sequences were analyzed for similarity with other known sequences using the BLAST programs at the National Center for Biotechnology Information ( www.ncbi.nlm.gov/blast ). .. Construction of Expression Vector and Recombinant Protein Expression The recombinant plasmid pMD18T-AS566 was digested with EcoRI and XhoI enzymes, and ligated into the EcoRI- or XhoI-digested expression vector, pET-30a(+) (Millipore). .. The pET-30a-566 plasmid was transformed intoE. coli BL21 (DE3) cells for the expression of the AS566 protein.

    Recombinant:

    Article Title: Cloning, Expression, and Purification of a New Antibacterial Substance Gene From Larvae ofMusca domestica(Diptera: Muscidae)
    Article Snippet: The generated sequences were analyzed for similarity with other known sequences using the BLAST programs at the National Center for Biotechnology Information ( www.ncbi.nlm.gov/blast ). .. Construction of Expression Vector and Recombinant Protein Expression The recombinant plasmid pMD18T-AS566 was digested with EcoRI and XhoI enzymes, and ligated into the EcoRI- or XhoI-digested expression vector, pET-30a(+) (Millipore). .. The pET-30a-566 plasmid was transformed intoE. coli BL21 (DE3) cells for the expression of the AS566 protein.

    Positron Emission Tomography:

    Article Title: Cloning, Expression, and Purification of a New Antibacterial Substance Gene From Larvae ofMusca domestica(Diptera: Muscidae)
    Article Snippet: The generated sequences were analyzed for similarity with other known sequences using the BLAST programs at the National Center for Biotechnology Information ( www.ncbi.nlm.gov/blast ). .. Construction of Expression Vector and Recombinant Protein Expression The recombinant plasmid pMD18T-AS566 was digested with EcoRI and XhoI enzymes, and ligated into the EcoRI- or XhoI-digested expression vector, pET-30a(+) (Millipore). .. The pET-30a-566 plasmid was transformed intoE. coli BL21 (DE3) cells for the expression of the AS566 protein.

    Article Title: A Novel Gene, Encoding 6-Hydroxy-3-Succinoylpyridine Hydroxylase, Involved in Nicotine Degradation by Pseudomonas putida Strain S16 ▿ Strain S16 ▿ ‡
    Article Snippet: .. The PCR product of hsp was digested with EcoRI and XhoI and ligated into pET-27b(+) (Novagen). .. Transformed E. coli BL21(DE3) cells with hsp were incubated in 500-ml flasks\chatn with 100 ml of LB medium containing 100 mg of kanamycin liter−1 until an optical density at 600 nm of 0.5 was reached.

    Methylation:

    Article Title: A Pseudo-Full Mutation Identified in Fragile X Assay Reveals a Novel Base Change Abolishing an EcoRI Restriction Site
    Article Snippet: The PCR products were fractionated on a 2% agarose gel and detected with ethidium bromide using UV illumination (Quantity One; Bio-Rad, Hercules, CA). .. For the standard Southern analysis, 6 μg of genomic DNA was double-digested with NruI (a methylation-sensitive enzyme, cutting only unmethylated, active X chromosome) and EcoRI (100 U of each enzyme at 37°C for 6 hours), electrophoresed on a 0.7% agarose gel, transferred to a nylon membrane, and hybridized with digoxiginin-labeled probe pFXa1NHE (Chemicon International, Temecula, CA). ..

    Agarose Gel Electrophoresis:

    Article Title: A Pseudo-Full Mutation Identified in Fragile X Assay Reveals a Novel Base Change Abolishing an EcoRI Restriction Site
    Article Snippet: The PCR products were fractionated on a 2% agarose gel and detected with ethidium bromide using UV illumination (Quantity One; Bio-Rad, Hercules, CA). .. For the standard Southern analysis, 6 μg of genomic DNA was double-digested with NruI (a methylation-sensitive enzyme, cutting only unmethylated, active X chromosome) and EcoRI (100 U of each enzyme at 37°C for 6 hours), electrophoresed on a 0.7% agarose gel, transferred to a nylon membrane, and hybridized with digoxiginin-labeled probe pFXa1NHE (Chemicon International, Temecula, CA). ..

    Southern Blot:

    Article Title: Conditional Cripto overexpression in satellite cells promotes myogenic commitment and enhances early regeneration
    Article Snippet: After germline transmission, heterozygous Tg:DsRedloxP/loxP Cripto-eGFP(A) and Tg:DsRedloxP/loxP Cripto-eGFP(B) mice colonies were maintained by crossing with wild-type FVB mice and named Tg:Cripto(A) and Tg:Cripto(B) , respectively. .. For Southern Blot analysis, 20 ug of genomic DNA was prepared from tail biopsies, digested with EcoRI and blotted on Immobilion-Ny+ (Millipore). .. The 32 P-labeled probe was a PCR amplified DNA fragment spanning the eGFP insert (546 bp) from the pDsRedloxP/loxP Cripto vector.

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  • 99
    Millipore ecor v sal i
    Ecor V Sal I, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor v sal i/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecor v sal i - by Bioz Stars, 2021-07
    99/100 stars
      Buy from Supplier

    93
    Millipore ecor v site
    Ecor V Site, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor v site/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecor v site - by Bioz Stars, 2021-07
    93/100 stars
      Buy from Supplier

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