ecor v  (Millipore)


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  • 93
    Name:
    EcoR V
    Description:
    EcoR V recognizes the sequence G AT↓ATC and generates fragments with blunt ends EcoR V is inhibited by the presence of N6 methyladenine at the site indicated on the recognition sequence EcoR V is not inhibited by the presence of 5 methylcytosine at the indicated site or 5 hydroxymethylcytosine EcoR V is an isoschizomer of Eco32 I
    Catalog Number:
    ecorv-ro
    Price:
    None
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    Structured Review

    Millipore ecor v
    EcoR V recognizes the sequence G AT↓ATC and generates fragments with blunt ends EcoR V is inhibited by the presence of N6 methyladenine at the site indicated on the recognition sequence EcoR V is not inhibited by the presence of 5 methylcytosine at the indicated site or 5 hydroxymethylcytosine EcoR V is an isoschizomer of Eco32 I
    https://www.bioz.com/result/ecor v/product/Millipore
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ecor v - by Bioz Stars, 2020-07
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Identification of HOXD4 Mutations in Spinal Extradural Arachnoid Cyst
    Article Snippet: .. Luciferase assay of the recombinant HOXD4 mutations We cloned the cDNA of the wild-type HOXD4 into the Xba I and EcoR V sites of the pFLAG-CMV-4 expression vector (Sigma Aldrich). .. We introduced the two HOXD4 mutations by site-directed mutagenesis using PrimeSTAR Mutagenesis Basal Kit (Takara).

    Article Title: Robust Therapeutic Efficacy of Matrix Metalloproteinase-2-Cleavable Fas-1-RGD Peptide Complex in Chronic Inflammatory Arthritis
    Article Snippet: .. Complementary DNA (cDNA) of the 4th fas-1 truncated for H1 and H2 sequences of murine βig-h3 (designated MFK00) were generated by polymerase chain reaction (PCR) and cloned into the EcoR V and Xho I sites of the pET-29b(+) plasmid (Novagen, Madison, WI). .. In order to produce a peptide complex consisting of MFK00 and RGD linked by the MMP-2 substrate (Gly-Pro-Leu-Gly-Val-Arg-Gly; GPLGVRG), cDNA of the RGD peptide and GPLGVRG was cloned into the EcoR I, Sal I, and XhoI sites of the pET-29b(+) plasmid.

    Amplification:

    Article Title: Cathepsin E Is a Marker of Gastric Differentiation and Signet-Ring Cell Carcinoma of Stomach: A Novel Suggestion on Gastric Tumorigenesis
    Article Snippet: .. The amplified fragment was inserted into the EcoR V sites of pT7Blue-T vector (Novagen, Darmstadt, Germany) to generate pT7Blue-Sox2. .. After double digestion with BamH I and Sal I, the obtained fragment was inserted into the BamH I/Xho I sites of pMXs-IRES-puro to generate pMXs-Sox2-IRES-puro.

    Article Title: Activation of site-specific DNA integration in human cells by a single chain integration host factor
    Article Snippet: .. The CAGGS promoter was amplified with Caggs-5-EcoR I, 5′-ccggaattctcttcttcttcgtattccttctct-3′; and Caggs-3-Nhe I, 5′-ctagctagcaggcagcgtcgcagcgactcc-3′, using pTriEx-1 as template (Novagen). .. PCR fragments were cut with NheI–BamHI or EcoRI–NheI, respectively, and cloned into the EcoRI–BamHI sites of pGEM7+ (Promega).

    Ligation:

    Article Title: A var Gene Upstream Element Controls Protein Synthesis at the Level of Translation Initiation in Plasmodium falciparum
    Article Snippet: .. This result suggested the presence of a novel EcoR V site upstream of a single copy of hdhfr-gfp (highlighted in purple). i, integration event; p, plasmid fragment. (C) Ligation-mediated PCR. gDNA from WR-selected 3D7/pBKmin C parasites was digested with EcoR V and Nco I and ligated into EcoR V/Nco I-digested pET-41 (EMD Biosciences). .. To amplify EcoR V/Nco I restriction fragments containing the hdhfr coding sequence, a primary PCR reaction was performed using T7 and hdhfr _R1 (R1) as forward and reverse primers, respectively.

    Generated:

    Article Title: Robust Therapeutic Efficacy of Matrix Metalloproteinase-2-Cleavable Fas-1-RGD Peptide Complex in Chronic Inflammatory Arthritis
    Article Snippet: .. Complementary DNA (cDNA) of the 4th fas-1 truncated for H1 and H2 sequences of murine βig-h3 (designated MFK00) were generated by polymerase chain reaction (PCR) and cloned into the EcoR V and Xho I sites of the pET-29b(+) plasmid (Novagen, Madison, WI). .. In order to produce a peptide complex consisting of MFK00 and RGD linked by the MMP-2 substrate (Gly-Pro-Leu-Gly-Val-Arg-Gly; GPLGVRG), cDNA of the RGD peptide and GPLGVRG was cloned into the EcoR I, Sal I, and XhoI sites of the pET-29b(+) plasmid.

    Luciferase:

    Article Title: Identification of HOXD4 Mutations in Spinal Extradural Arachnoid Cyst
    Article Snippet: .. Luciferase assay of the recombinant HOXD4 mutations We cloned the cDNA of the wild-type HOXD4 into the Xba I and EcoR V sites of the pFLAG-CMV-4 expression vector (Sigma Aldrich). .. We introduced the two HOXD4 mutations by site-directed mutagenesis using PrimeSTAR Mutagenesis Basal Kit (Takara).

    Expressing:

    Article Title: Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 ?-glucosidase
    Article Snippet: .. The Gateway Conversion cassette A was ligated into the EcoR V site of pET32a (+) (Novagen, Madison, WI) according to the Invitrogen Gateway Conversion Kit directions, to create the pET32a (+)/DEST Gateway expression vector. .. The cDNA encoding the mature protein of the Os4bglu12 was PCR amplified using cDNA cloned as the template with the Os4bglu12matNcoIf (5'-CACCATGGCCTACAATAGCGCCGGCGAG-3') and Os4bglu12stopr (5'-ATCATTTCAGGAGGAACTTCTTG-3') primers and Pfu DNA polymerase to introduce a directional cloning site at the 5' end.

    Article Title: Identification of HOXD4 Mutations in Spinal Extradural Arachnoid Cyst
    Article Snippet: .. Luciferase assay of the recombinant HOXD4 mutations We cloned the cDNA of the wild-type HOXD4 into the Xba I and EcoR V sites of the pFLAG-CMV-4 expression vector (Sigma Aldrich). .. We introduced the two HOXD4 mutations by site-directed mutagenesis using PrimeSTAR Mutagenesis Basal Kit (Takara).

    Polymerase Chain Reaction:

    Article Title: Crystal Structure of StnA for the Biosynthesis of Antitumor Drug Streptonigrin Reveals a Unique Substrate Binding Mode
    Article Snippet: .. The PCR product was ligated into the pBluescript II SK (+) (Stratagene) vector and digested with EcoR V. The recombinant plasmid was digested with BamH I and Xho I to generate a 1.1 kb fragment, and inserted into the corresponding site of pET28a (Novagen) vector with both N- and C-terminal His-tag. .. The StnA point mutants were constructed by the whole-plasmid PCR and Dpn I digestion method, and were verified by plasmid sequencing.

    Article Title: Robust Therapeutic Efficacy of Matrix Metalloproteinase-2-Cleavable Fas-1-RGD Peptide Complex in Chronic Inflammatory Arthritis
    Article Snippet: .. Complementary DNA (cDNA) of the 4th fas-1 truncated for H1 and H2 sequences of murine βig-h3 (designated MFK00) were generated by polymerase chain reaction (PCR) and cloned into the EcoR V and Xho I sites of the pET-29b(+) plasmid (Novagen, Madison, WI). .. In order to produce a peptide complex consisting of MFK00 and RGD linked by the MMP-2 substrate (Gly-Pro-Leu-Gly-Val-Arg-Gly; GPLGVRG), cDNA of the RGD peptide and GPLGVRG was cloned into the EcoR I, Sal I, and XhoI sites of the pET-29b(+) plasmid.

    Article Title: A var Gene Upstream Element Controls Protein Synthesis at the Level of Translation Initiation in Plasmodium falciparum
    Article Snippet: .. This result suggested the presence of a novel EcoR V site upstream of a single copy of hdhfr-gfp (highlighted in purple). i, integration event; p, plasmid fragment. (C) Ligation-mediated PCR. gDNA from WR-selected 3D7/pBKmin C parasites was digested with EcoR V and Nco I and ligated into EcoR V/Nco I-digested pET-41 (EMD Biosciences). .. To amplify EcoR V/Nco I restriction fragments containing the hdhfr coding sequence, a primary PCR reaction was performed using T7 and hdhfr _R1 (R1) as forward and reverse primers, respectively.

    Recombinant:

    Article Title: Identification of HOXD4 Mutations in Spinal Extradural Arachnoid Cyst
    Article Snippet: .. Luciferase assay of the recombinant HOXD4 mutations We cloned the cDNA of the wild-type HOXD4 into the Xba I and EcoR V sites of the pFLAG-CMV-4 expression vector (Sigma Aldrich). .. We introduced the two HOXD4 mutations by site-directed mutagenesis using PrimeSTAR Mutagenesis Basal Kit (Takara).

    Article Title: Crystal Structure of StnA for the Biosynthesis of Antitumor Drug Streptonigrin Reveals a Unique Substrate Binding Mode
    Article Snippet: .. The PCR product was ligated into the pBluescript II SK (+) (Stratagene) vector and digested with EcoR V. The recombinant plasmid was digested with BamH I and Xho I to generate a 1.1 kb fragment, and inserted into the corresponding site of pET28a (Novagen) vector with both N- and C-terminal His-tag. .. The StnA point mutants were constructed by the whole-plasmid PCR and Dpn I digestion method, and were verified by plasmid sequencing.

    Positron Emission Tomography:

    Article Title: Robust Therapeutic Efficacy of Matrix Metalloproteinase-2-Cleavable Fas-1-RGD Peptide Complex in Chronic Inflammatory Arthritis
    Article Snippet: .. Complementary DNA (cDNA) of the 4th fas-1 truncated for H1 and H2 sequences of murine βig-h3 (designated MFK00) were generated by polymerase chain reaction (PCR) and cloned into the EcoR V and Xho I sites of the pET-29b(+) plasmid (Novagen, Madison, WI). .. In order to produce a peptide complex consisting of MFK00 and RGD linked by the MMP-2 substrate (Gly-Pro-Leu-Gly-Val-Arg-Gly; GPLGVRG), cDNA of the RGD peptide and GPLGVRG was cloned into the EcoR I, Sal I, and XhoI sites of the pET-29b(+) plasmid.

    Article Title: A var Gene Upstream Element Controls Protein Synthesis at the Level of Translation Initiation in Plasmodium falciparum
    Article Snippet: .. This result suggested the presence of a novel EcoR V site upstream of a single copy of hdhfr-gfp (highlighted in purple). i, integration event; p, plasmid fragment. (C) Ligation-mediated PCR. gDNA from WR-selected 3D7/pBKmin C parasites was digested with EcoR V and Nco I and ligated into EcoR V/Nco I-digested pET-41 (EMD Biosciences). .. To amplify EcoR V/Nco I restriction fragments containing the hdhfr coding sequence, a primary PCR reaction was performed using T7 and hdhfr _R1 (R1) as forward and reverse primers, respectively.

    Plasmid Preparation:

    Article Title: Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 ?-glucosidase
    Article Snippet: .. The Gateway Conversion cassette A was ligated into the EcoR V site of pET32a (+) (Novagen, Madison, WI) according to the Invitrogen Gateway Conversion Kit directions, to create the pET32a (+)/DEST Gateway expression vector. .. The cDNA encoding the mature protein of the Os4bglu12 was PCR amplified using cDNA cloned as the template with the Os4bglu12matNcoIf (5'-CACCATGGCCTACAATAGCGCCGGCGAG-3') and Os4bglu12stopr (5'-ATCATTTCAGGAGGAACTTCTTG-3') primers and Pfu DNA polymerase to introduce a directional cloning site at the 5' end.

    Article Title: Identification of HOXD4 Mutations in Spinal Extradural Arachnoid Cyst
    Article Snippet: .. Luciferase assay of the recombinant HOXD4 mutations We cloned the cDNA of the wild-type HOXD4 into the Xba I and EcoR V sites of the pFLAG-CMV-4 expression vector (Sigma Aldrich). .. We introduced the two HOXD4 mutations by site-directed mutagenesis using PrimeSTAR Mutagenesis Basal Kit (Takara).

    Article Title: Cathepsin E Is a Marker of Gastric Differentiation and Signet-Ring Cell Carcinoma of Stomach: A Novel Suggestion on Gastric Tumorigenesis
    Article Snippet: .. The amplified fragment was inserted into the EcoR V sites of pT7Blue-T vector (Novagen, Darmstadt, Germany) to generate pT7Blue-Sox2. .. After double digestion with BamH I and Sal I, the obtained fragment was inserted into the BamH I/Xho I sites of pMXs-IRES-puro to generate pMXs-Sox2-IRES-puro.

    Article Title: Crystal Structure of StnA for the Biosynthesis of Antitumor Drug Streptonigrin Reveals a Unique Substrate Binding Mode
    Article Snippet: .. The PCR product was ligated into the pBluescript II SK (+) (Stratagene) vector and digested with EcoR V. The recombinant plasmid was digested with BamH I and Xho I to generate a 1.1 kb fragment, and inserted into the corresponding site of pET28a (Novagen) vector with both N- and C-terminal His-tag. .. The StnA point mutants were constructed by the whole-plasmid PCR and Dpn I digestion method, and were verified by plasmid sequencing.

    Article Title: Robust Therapeutic Efficacy of Matrix Metalloproteinase-2-Cleavable Fas-1-RGD Peptide Complex in Chronic Inflammatory Arthritis
    Article Snippet: .. Complementary DNA (cDNA) of the 4th fas-1 truncated for H1 and H2 sequences of murine βig-h3 (designated MFK00) were generated by polymerase chain reaction (PCR) and cloned into the EcoR V and Xho I sites of the pET-29b(+) plasmid (Novagen, Madison, WI). .. In order to produce a peptide complex consisting of MFK00 and RGD linked by the MMP-2 substrate (Gly-Pro-Leu-Gly-Val-Arg-Gly; GPLGVRG), cDNA of the RGD peptide and GPLGVRG was cloned into the EcoR I, Sal I, and XhoI sites of the pET-29b(+) plasmid.

    Article Title: A var Gene Upstream Element Controls Protein Synthesis at the Level of Translation Initiation in Plasmodium falciparum
    Article Snippet: .. This result suggested the presence of a novel EcoR V site upstream of a single copy of hdhfr-gfp (highlighted in purple). i, integration event; p, plasmid fragment. (C) Ligation-mediated PCR. gDNA from WR-selected 3D7/pBKmin C parasites was digested with EcoR V and Nco I and ligated into EcoR V/Nco I-digested pET-41 (EMD Biosciences). .. To amplify EcoR V/Nco I restriction fragments containing the hdhfr coding sequence, a primary PCR reaction was performed using T7 and hdhfr _R1 (R1) as forward and reverse primers, respectively.

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  • 93
    Millipore ecor v
    Ecor V, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor v/product/Millipore
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    ecor v - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    85
    Millipore ecor v digested pbluescript sk plasmid
    Ecor V Digested Pbluescript Sk Plasmid, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor v digested pbluescript sk plasmid/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecor v digested pbluescript sk plasmid - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    98
    Millipore ecor v nco i digested pet 41
    Ecor V Nco I Digested Pet 41, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor v nco i digested pet 41/product/Millipore
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecor v nco i digested pet 41 - by Bioz Stars, 2020-07
    98/100 stars
      Buy from Supplier

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