ecor v nco i digested pet 41  (Millipore)


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    Name:
    EcoR I
    Description:
    EcoR I recognizes the sequence G↓ A ATT C and generates fragments with 5 cohesive termini EcoR I is inhibited if N6 methyladenine occurs at either or both A residues in the recognition site it is also inhibited if 5 methylcytosine occurs at the site indicated EcoR I is an isoschizomer to Rsr I
    Catalog Number:
    ecori-ro
    Price:
    None
    Applications:
    EcoR I from Escherichia coli BS5 has been used to digest bacterial DNA.
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    Structured Review

    Millipore ecor v nco i digested pet 41
    EcoR I recognizes the sequence G↓ A ATT C and generates fragments with 5 cohesive termini EcoR I is inhibited if N6 methyladenine occurs at either or both A residues in the recognition site it is also inhibited if 5 methylcytosine occurs at the site indicated EcoR I is an isoschizomer to Rsr I
    https://www.bioz.com/result/ecor v nco i digested pet 41/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecor v nco i digested pet 41 - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Positron Emission Tomography:

    Article Title: Cloning, Expression, and Purification of a New Antibacterial Substance Gene From Larvae ofMusca domestica(Diptera: Muscidae)
    Article Snippet: .. Construction of Expression Vector and Recombinant Protein Expression The recombinant plasmid pMD18T-AS566 was digested with EcoRI and XhoI enzymes, and ligated into the EcoRI- or XhoI-digested expression vector, pET-30a(+) (Millipore). .. The pET-30a-566 plasmid was transformed intoE. coli BL21 (DE3) cells for the expression of the AS566 protein.

    Agarose Gel Electrophoresis:

    Article Title: A Pseudo-Full Mutation Identified in Fragile X Assay Reveals a Novel Base Change Abolishing an EcoRI Restriction Site
    Article Snippet: .. For the standard Southern analysis, 6 μg of genomic DNA was double-digested with NruI (a methylation-sensitive enzyme, cutting only unmethylated, active X chromosome) and EcoRI (100 U of each enzyme at 37°C for 6 hours), electrophoresed on a 0.7% agarose gel, transferred to a nylon membrane, and hybridized with digoxiginin-labeled probe pFXa1NHE (Chemicon International, Temecula, CA). ..

    Article Title: Origin-independent plasmid replication occurs in vaccinia virus cytoplasmic factories and requires all five known poxvirus replication factors
    Article Snippet: .. Southern blotting DNA (2 μg) was digested with EcoR I and Dpn I or Mbo I, resolved on a 0.8% agarose gel, and transferred to Immobilon-Ny+ (Millipore) transfer membrane. .. Southern blotting was carried out as described by Maniatis [ ].

    Southern Blot:

    Article Title: Origin-independent plasmid replication occurs in vaccinia virus cytoplasmic factories and requires all five known poxvirus replication factors
    Article Snippet: .. Southern blotting DNA (2 μg) was digested with EcoR I and Dpn I or Mbo I, resolved on a 0.8% agarose gel, and transferred to Immobilon-Ny+ (Millipore) transfer membrane. .. Southern blotting was carried out as described by Maniatis [ ].

    Methylation:

    Article Title: A Pseudo-Full Mutation Identified in Fragile X Assay Reveals a Novel Base Change Abolishing an EcoRI Restriction Site
    Article Snippet: .. For the standard Southern analysis, 6 μg of genomic DNA was double-digested with NruI (a methylation-sensitive enzyme, cutting only unmethylated, active X chromosome) and EcoRI (100 U of each enzyme at 37°C for 6 hours), electrophoresed on a 0.7% agarose gel, transferred to a nylon membrane, and hybridized with digoxiginin-labeled probe pFXa1NHE (Chemicon International, Temecula, CA). ..

    Construct:

    Article Title: Use of the Plant Defense Protein Osmotin To Identify Fusarium oxysporum Genes That Control Cell Wall Properties ▿ Genes That Control Cell Wall Properties ▿ ‡
    Article Snippet: .. To construct p for3 ::HYG, a 0.7-kb fragment containing the 3′ part of the FOR3 ORF (+3205 bp to +3941 bp) was amplified from F. oxysporum genomic DNA by PCR using the primer pair 5′-ATCGAGTCTTGCCGACGAT-3′/5′-TGAGATCCGTCTTCAGGATC-3′ and inserted into the EcoRV site of the pSTblue-1 vector (Novagen, Madison, WI). .. After sequencing, to establish the fidelity of the PCR and the direction of the insert, a 1.4-kb SalI fragment of plasmid pCB1003 (Fungal Genetics Stock Center) containing a hygromycin resistance gene cassette was inserted into the SalI site on this plasmid, yielding a construct that contained the hygromycin gene cassette fused to the 5′ end of the FOR3 fragment (+3205 bp to +3941 bp).

    FLAG-tag:

    Article Title: A set of vectors for introduction of antibiotic resistance genes by in vitro Cre-mediated recombination
    Article Snippet: .. Target vectors In order to prepare the pT-FLAG vector, we digested phrGFP vector (Stratagene) by NheI and EcoRI and replaced the GFP ORF by the oligonucleotide duplex encoding the FLAG-tag (oligo1: 5'-CTAGCCCATGGATTACAAAGACGATGACGATAAACCTAGCTTCG; oligo2: 5'-AATTCGAAGCTAGGTTTATCGTCATCGTCTTTGTAATCCATGGG) (Sigma, St. Louis, MO, USA). .. Then LoxP-site coupled to ampicillin resistance gene was isolated from pT-FLAG plasmid by BspHI digestion and cloned into phRL-TK (Promega, Madison, WI, USA) or pBlueScriptII (Stratagene) digested by BspHI.

    Amplification:

    Article Title: Use of the Plant Defense Protein Osmotin To Identify Fusarium oxysporum Genes That Control Cell Wall Properties ▿ Genes That Control Cell Wall Properties ▿ ‡
    Article Snippet: .. To construct p for3 ::HYG, a 0.7-kb fragment containing the 3′ part of the FOR3 ORF (+3205 bp to +3941 bp) was amplified from F. oxysporum genomic DNA by PCR using the primer pair 5′-ATCGAGTCTTGCCGACGAT-3′/5′-TGAGATCCGTCTTCAGGATC-3′ and inserted into the EcoRV site of the pSTblue-1 vector (Novagen, Madison, WI). .. After sequencing, to establish the fidelity of the PCR and the direction of the insert, a 1.4-kb SalI fragment of plasmid pCB1003 (Fungal Genetics Stock Center) containing a hygromycin resistance gene cassette was inserted into the SalI site on this plasmid, yielding a construct that contained the hygromycin gene cassette fused to the 5′ end of the FOR3 fragment (+3205 bp to +3941 bp).

    DNA Sequencing:

    Article Title: Identification and characterization of duck plague virus glycoprotein C gene and gene product
    Article Snippet: .. After verified by PCR, restriction analysis and DNA sequencing (TaKaRa), the gC gene fragment, which was obtained by digestion of pMD18-T/gC with EcoRI and XhoI, was ligated into prokaryotic vector pET32a(+) (Novagen) (Figure ), which was digested previously with the same restriction enzymes. .. The recombinant plasmid, named pET32a-gC, was confirmed by PCR, restriction enzyme digestion and DNA sequencing (TaKaRa).

    Expressing:

    Article Title: Cloning, Expression, and Purification of a New Antibacterial Substance Gene From Larvae ofMusca domestica(Diptera: Muscidae)
    Article Snippet: .. Construction of Expression Vector and Recombinant Protein Expression The recombinant plasmid pMD18T-AS566 was digested with EcoRI and XhoI enzymes, and ligated into the EcoRI- or XhoI-digested expression vector, pET-30a(+) (Millipore). .. The pET-30a-566 plasmid was transformed intoE. coli BL21 (DE3) cells for the expression of the AS566 protein.

    Polymerase Chain Reaction:

    Article Title: Use of the Plant Defense Protein Osmotin To Identify Fusarium oxysporum Genes That Control Cell Wall Properties ▿ Genes That Control Cell Wall Properties ▿ ‡
    Article Snippet: .. To construct p for3 ::HYG, a 0.7-kb fragment containing the 3′ part of the FOR3 ORF (+3205 bp to +3941 bp) was amplified from F. oxysporum genomic DNA by PCR using the primer pair 5′-ATCGAGTCTTGCCGACGAT-3′/5′-TGAGATCCGTCTTCAGGATC-3′ and inserted into the EcoRV site of the pSTblue-1 vector (Novagen, Madison, WI). .. After sequencing, to establish the fidelity of the PCR and the direction of the insert, a 1.4-kb SalI fragment of plasmid pCB1003 (Fungal Genetics Stock Center) containing a hygromycin resistance gene cassette was inserted into the SalI site on this plasmid, yielding a construct that contained the hygromycin gene cassette fused to the 5′ end of the FOR3 fragment (+3205 bp to +3941 bp).

    Article Title: Identification and characterization of duck plague virus glycoprotein C gene and gene product
    Article Snippet: .. After verified by PCR, restriction analysis and DNA sequencing (TaKaRa), the gC gene fragment, which was obtained by digestion of pMD18-T/gC with EcoRI and XhoI, was ligated into prokaryotic vector pET32a(+) (Novagen) (Figure ), which was digested previously with the same restriction enzymes. .. The recombinant plasmid, named pET32a-gC, was confirmed by PCR, restriction enzyme digestion and DNA sequencing (TaKaRa).

    Recombinant:

    Article Title: Cloning, Expression, and Purification of a New Antibacterial Substance Gene From Larvae ofMusca domestica(Diptera: Muscidae)
    Article Snippet: .. Construction of Expression Vector and Recombinant Protein Expression The recombinant plasmid pMD18T-AS566 was digested with EcoRI and XhoI enzymes, and ligated into the EcoRI- or XhoI-digested expression vector, pET-30a(+) (Millipore). .. The pET-30a-566 plasmid was transformed intoE. coli BL21 (DE3) cells for the expression of the AS566 protein.

    Plasmid Preparation:

    Article Title: A set of vectors for introduction of antibiotic resistance genes by in vitro Cre-mediated recombination
    Article Snippet: .. Target vectors In order to prepare the pT-FLAG vector, we digested phrGFP vector (Stratagene) by NheI and EcoRI and replaced the GFP ORF by the oligonucleotide duplex encoding the FLAG-tag (oligo1: 5'-CTAGCCCATGGATTACAAAGACGATGACGATAAACCTAGCTTCG; oligo2: 5'-AATTCGAAGCTAGGTTTATCGTCATCGTCTTTGTAATCCATGGG) (Sigma, St. Louis, MO, USA). .. Then LoxP-site coupled to ampicillin resistance gene was isolated from pT-FLAG plasmid by BspHI digestion and cloned into phRL-TK (Promega, Madison, WI, USA) or pBlueScriptII (Stratagene) digested by BspHI.

    Article Title: Cloning, Expression, and Purification of a New Antibacterial Substance Gene From Larvae ofMusca domestica(Diptera: Muscidae)
    Article Snippet: .. Construction of Expression Vector and Recombinant Protein Expression The recombinant plasmid pMD18T-AS566 was digested with EcoRI and XhoI enzymes, and ligated into the EcoRI- or XhoI-digested expression vector, pET-30a(+) (Millipore). .. The pET-30a-566 plasmid was transformed intoE. coli BL21 (DE3) cells for the expression of the AS566 protein.

    Article Title: Use of the Plant Defense Protein Osmotin To Identify Fusarium oxysporum Genes That Control Cell Wall Properties ▿ Genes That Control Cell Wall Properties ▿ ‡
    Article Snippet: .. To construct p for3 ::HYG, a 0.7-kb fragment containing the 3′ part of the FOR3 ORF (+3205 bp to +3941 bp) was amplified from F. oxysporum genomic DNA by PCR using the primer pair 5′-ATCGAGTCTTGCCGACGAT-3′/5′-TGAGATCCGTCTTCAGGATC-3′ and inserted into the EcoRV site of the pSTblue-1 vector (Novagen, Madison, WI). .. After sequencing, to establish the fidelity of the PCR and the direction of the insert, a 1.4-kb SalI fragment of plasmid pCB1003 (Fungal Genetics Stock Center) containing a hygromycin resistance gene cassette was inserted into the SalI site on this plasmid, yielding a construct that contained the hygromycin gene cassette fused to the 5′ end of the FOR3 fragment (+3205 bp to +3941 bp).

    Article Title: Identification and characterization of duck plague virus glycoprotein C gene and gene product
    Article Snippet: .. After verified by PCR, restriction analysis and DNA sequencing (TaKaRa), the gC gene fragment, which was obtained by digestion of pMD18-T/gC with EcoRI and XhoI, was ligated into prokaryotic vector pET32a(+) (Novagen) (Figure ), which was digested previously with the same restriction enzymes. .. The recombinant plasmid, named pET32a-gC, was confirmed by PCR, restriction enzyme digestion and DNA sequencing (TaKaRa).

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  • 99
    Millipore ecor v nco i digested pet 41
    Ecor V Nco I Digested Pet 41, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor v nco i digested pet 41/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ecor v nco i digested pet 41 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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